Reviewed, UniProtKB/Swiss-Prot Q15014 (MO4L2_HUMAN)

Names and origin

Protein names

Recommended name:
    Mortality factor 4-like protein 2
Alternative name(s):
    MORF-related gene X protein
    Transcription factor-like protein MRGX
    Protein MSL3-2

Gene names

Name:	MORF4L2
Synonyms:	KIAA0026, MRGX

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.
Subunit structure	Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. The NuA4 complex interacts with MYC and the adenovirus E1A protein. MORF4L1 may also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. Component of the MSIN3A histone deacetylase complex, which includes SIN3A, HDAC2, ARID4B, MORF4L1, RBBP4/RbAp48, and RBBP7/RbAp46. MORF4L2 interacts with MRFAP1 and RB1. MORF4L2 may also interact with one or more as yet undefined members of the TLE (transducin-like enhancer of split) family of transcriptional repressors. Ref.10 Ref.11
Subcellular location	Nucleus.
Sequence similarities	Belongs to the MRG family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Growth regulation Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell growth Non-traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Ref.11 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDR2	Q01850	4	EBI-399257,EBI-1181367
HDAC1	Q13547	1	EBI-399257,EBI-301834
MPDU1	O75352	1	EBI-399257,EBI-1046501
MRFAP1	Q9Y605	2	EBI-399257,EBI-995714
PITPNA	Q00169	1	EBI-399257,EBI-1042490
RB1	P06400	1	EBI-399257,EBI-491274

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Mortality factor 4-like protein 2		PRO_0000088768
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.12
	Modified residue	262	1	Phosphotyrosine By similarity
	Modified residue	267	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	132 – 136	5	Missing: Abrogates both transcriptional activation and repression by MORF4L2.
	Mutagenesis	263	1	L → A: Abrogates both transcriptional activation and repression by MORF4L2. Ref.11

Sequences

	Sequence		Length	Mass (Da)
	Q15014-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C9EFF517C76A565D	FASTA	288	32,308
	10 20 30 40 50 60 MSSRKQGSQP RGQQSAEEEN FKKPTRSNMQ RSKMRGASSG KKTAGPQQKN LEPALPGRWG 70 80 90 100 110 120 GRSAENPPSG SVRKTRKNKQ KTPGNGDGGS TSEAPQPPRK KRARADPTVE SEEAFKNRME 130 140 150 160 170 180 VKVKIPEELK PWLVEDWDLV TRQKQLFQLP AKKNVDAILE EYANCKKSQG NVDNKEYAVN 190 200 210 220 230 240 EVVAGIKEYF NVMLGTQLLY KFERPQYAEI LLAHPDAPMS QVYGAPHLLR LFVRIGAMLA 250 260 270 280 YTPLDEKSLA LLLGYLHDFL KYLAKNSASL FTASDYKVAS AEYHRKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes." Bertram M.J., Berube N.G., Hang-Swanson X., Ran Q., Leung J.K., Bryce S., Spurgers K., Bick R.J., Baldini A., Ning Y., Clark L.J., Parkinson E.K., Barrett J.C., Smith J.R., Pereira-Smith O.M. Mol. Cell. Biol. 19:1479-1485(1999) [PubMed: 9891081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Two human homologs of the Drosophila dosage compensation gene msl-3 are located on the X chromosome." D'Esposito M., Cocchia M., Matarazzo M.R., Macmillan S., Mazzarella R. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed: 7584026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Placenta.
[8]	"Mortality factor related gene X 102 5'." Myokai F., Oyama M. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.
[9]	"Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex." Cai Y., Jin J., Tomomori-Sato C., Sato S., Sorokina I., Parmely T.J., Conaway R.C., Conaway J.W. J. Biol. Chem. 278:42733-42736(2003) [PubMed: 12963728] [Abstract] Cited for: PROTEIN SEQUENCE OF 12-22; 82-99; 105-116; 154-166; 176-187; 188-201; 235-247 AND 266-277, IDENTIFICATION IN NUA4 COMPLEX.
[10]	"Role for the mortality factors MORF4, MRGX, and MRG15 in transcriptional repression via associations with Pf1, mSin3A, and transducin-like enhancer of Split." Yochum G.S., Ayer D.E. Mol. Cell. Biol. 22:7868-7876(2002) [PubMed: 12391155] [Abstract] Cited for: INTERACTION WITH SIN3A AND TLE FAMILY MEMBERS.
[11]	"MRGX is a novel transcriptional regulator that exhibits activation or repression of the B-myb promoter in a cell type-dependent manner." Tominaga K., Leung J.K., Rookard P., Echigo J., Smith J.R., Pereira-Smith O.M. J. Biol. Chem. 278:49618-49624(2003) [PubMed: 14506250] [Abstract] Cited for: INTERACTION WITH MRFAP1 AND RB1, MUTAGENESIS OF 132-TRP--ASP-136 AND LEU-263.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, MASS SPECTROMETRY.
[13]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF100620 mRNA. Translation: AAD29873.1. AF167174 mRNA. Translation: AAF80855.1. D14812 mRNA. Translation: BAA03553.1. AK056012 mRNA. Translation: BAG51604.1. AL049610 Genomic DNA. Translation: CAB55701.1. CH471190 Genomic DNA. Translation: EAW54698.1. BC056899 mRNA. Translation: AAH56899.1. BC093013 mRNA. Translation: AAH93013.1. AB050778 mRNA. Translation: BAC22659.1.
IPI	IPI00014174.
RefSeq	NP_001135890.1. NP_001135891.1. NP_001135892.1. NP_001135893.1. NP_001135894.1. NP_001135895.1. NP_001135896.1. NP_001135897.1. NP_001135898.1. NP_001135899.1. NP_001135900.1. NP_001135901.1. NP_001135902.1. NP_001135903.1. NP_001135904.1. NP_036418.1.
UniGene	Hs.326387 Hs.441076 Hs.597035
3D structure databases
SMR	Q15014. Positions 120-286.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15014. 11 interactions.
STRING	Q15014.
PTM databases
PhosphoSite	Q15014.
Proteomic databases
PRIDE	Q15014.
Genome annotation databases
Ensembl	ENST00000340782; ENSP00000341966; ENSG00000123562; Homo sapiens. [Genome view] ENST00000360458; ENSP00000353643; ENSG00000123562; Homo sapiens. [Genome view] ENST00000372619; ENSP00000361702; ENSG00000123562; Homo sapiens. [Genome view] ENST00000422154; ENSP00000394417; ENSG00000123562; Homo sapiens. [Genome view] ENST00000423833; ENSP00000416120; ENSG00000123562; Homo sapiens. [Genome view] ENST00000428596; ENSP00000400366; ENSG00000123562; Homo sapiens. [Genome view] ENST00000433176; ENSP00000415476; ENSG00000123562; Homo sapiens. [Genome view] ENST00000438718; ENSP00000400654; ENSG00000123562; Homo sapiens. [Genome view] ENST00000441076; ENSP00000391969; ENSG00000123562; Homo sapiens. [Genome view] ENST00000448047; ENSP00000412096; ENSG00000123562; Homo sapiens. [Genome view] ENST00000451301; ENSP00000410532; ENSG00000123562; Homo sapiens. [Genome view]
GeneID	9643.
KEGG	hsa:9643.
UCSC	uc004ekw.1. human.
Organism-specific databases
CTD	9643.
GeneCards	GC0XM102817.
H-InvDB	HIX0016954.
HGNC	HGNC:16849. MORF4L2.
MIM	300409. gene.
PharmGKB	PA134925837.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11427.
HOGENOM	HBG396389.
HOVERGEN	Q15014.
InParanoid	Q15014.
OMA	EDNFKKP.
OrthoDB	EOG915JZQ.
PhylomeDB	Q15014.
Gene expression databases
ArrayExpress	Q15014.
Bgee	Q15014.
CleanEx	HS_MORF4L2.
Genevestigator	Q15014.
GermOnline	ENSG00000123562. Homo sapiens.
Family and domain databases
InterPro	IPR008676. MRG. [Graphical view]
PANTHER	PTHR10880. MRG. 1 hit.
Pfam	PF05712. MRG. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	36195.
SOURCE	Search...

Entry information

Entry name

MO4L2_HUMAN

Accession

Primary (citable) accession number: Q15014
Secondary accession number(s): B3KP92, Q567V0, Q8J026

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.