Q15013 (MD2BP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAD2L1-binding protein

Alternative name(s):
Caught by MAD2 protein

Gene names

Name:	MAD2L1BP
Synonyms:	CMT2, KIAA0110

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. Ref.6 Ref.8
Subunit structure	Interacts with MAD2L1. Ref.6 Ref.8
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle. Note: During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle. Ref.6 Ref.8
Developmental stage	During the cell cycle, levels increase and then remain constant until late mitosis after which they drop. Ref.6
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.7
Sequence similarities	Belongs to the MAD2L1BP family.
Sequence caution	The sequence BAG59665.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitotic cell cycle checkpoint Inferred from mutant phenotype Ref.8. Source: UniProtKB regulation of exit from mitosis Inferred from mutant phenotype Ref.6. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay. Source: UniProtKB spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.8. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAD2L1	Q13257	4	EBI-712181,EBI-78203
TRIP13	Q15645	5	EBI-712181,EBI-358993

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 274

274

MAD2L1-binding protein

PRO_0000096310

Regions

Region

45 – 78

34

Interaction with MAD2L1

Amino acid modifications

Modified residue

29

1

Phosphoserine Ref.7

Modified residue

39

1

Phosphothreonine Ref.7

Experimental info

Mutagenesis

83

1

Q → A: Loss of interaction with MAD2L1 and disruption of ability to overcome spindle checkpoint-dependent mitotic arrest; when associated with A-191. Ref.8

Mutagenesis

191

1

F → A: Loss of interaction with MAD2L1 and disruption of ability to overcome spindle checkpoint-dependent mitotic arrest; when associated with A-83. Ref.8

Sequence conflict

133

1

S → G in BAG59665. Ref.2

Sequence conflict

274

1

E → D in CAG33174. Ref.3

Secondary structure

1

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274

Helix Strand Turn

Details...

Beta strand

55 – 59

5

Helix

67 – 83

17

Beta strand

86 – 89

4

Helix

91 – 94

4

Helix

126 – 144

19

Beta strand

149 – 156

8

Beta strand

158 – 160

3

Beta strand

162 – 168

7

Helix

183 – 196

14

Turn

197 – 200

4

Beta strand

210 – 218

9

Beta strand

224 – 229

6

Beta strand

239 – 246

8

Turn

254 – 259

6

Beta strand

260 – 264

5

Sequences

	Sequence		Length	Mass (Da)
	Q15013 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5C49FBE1F456105C	FASTA	274	31,052
	10 20 30 40 50 60 MAAPEAEVLS SAAVPDLEWY EKSEETHASQ IELLETSSTQ EPLNASEAFC PRDCMVPVVF 70 80 90 100 110 120 PGPVSQEGCC QFTCELLKHI MYQRQQLPLP YEQLKHFYRK PSPQAEEMLK KKPRATTEVS 130 140 150 160 170 180 SRKCQQALAE LESVLSHLED FFARTLVPRV LILLGGNALS PKEFYELDLS LLAPYSVDQS 190 200 210 220 230 240 LSTAACLRRL FRAIFMADAF SELQAPPLMG TVVMAQGHRN CGEDWFRPKL NYRVPSRGHK 250 260 270 LTVTLSCGRP SIRTTAWEDY IWFQAPVTFK GFRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed: 7788527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"Identification of a MAD2-binding protein, CMT2, and its role in mitosis." Habu T., Kim S.H., Weinstein J., Matsumoto T. EMBO J. 21:6419-6428(2002) [PubMed: 12456649] [Abstract] Cited for: POSSIBLE FUNCTION, INTERACTION WITH MAD2L1, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29 AND THR-39, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"p31comet blocks Mad2 activation through structural mimicry." Yang M., Li B., Tomchick D.R., Machius M., Rizo J., Yu H., Luo X. Cell 131:744-755(2007) [PubMed: 18022368] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 36-274 IN COMPLEX WITH MAD2L1, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MAD2L1, MUTAGENESIS OF GLN-83 AND PHE-191.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D14811 mRNA. Translation: BAA03552.1.
AK297172 mRNA. Translation: BAG59665.1. Different initiation.
CR456893 mRNA. Translation: CAG33174.1.
AL136131 Genomic DNA. Translation: CAC19507.1.
BC002904 mRNA. Translation: AAH02904.1.

IPI

IPI00014173.

RefSeq

NP_001003690.1. NM_001003690.1.
NP_055443.1. NM_014628.2.

UniGene

Hs.122346.
Hs.715485.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QYF	X-ray	2.30	B/D	36-274	[»]

ProteinModelPortal

Q15013.

SMR

Q15013. Positions 54-273.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15013. 11 interactions.

MINT

MINT-1368732.

STRING

Q15013.

PTM databases

PhosphoSite

Q15013.

Polymorphism databases

DMDM

3123048.

Proteomic databases

PRIDE

Q15013.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000372171; ENSP00000361244; ENSG00000124688.
ENST00000426254; ENSP00000409010; ENSG00000124688.

GeneID

9587.

KEGG

hsa:9587.

UCSC

uc003ovv.1. human.

Organism-specific databases

CTD

9587.

GeneCards

GC06P043597.

HGNC

HGNC:21059. MAD2L1BP.

neXtProt

NX_Q15013.

PharmGKB

PA134911020.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08208.

GeneTree

ENSGT00390000003812.

HOVERGEN

HBG052442.

PhylomeDB

Q15013.

Gene expression databases

ArrayExpress

Q15013.

Bgee

Q15013.

CleanEx

HS_MAD2L1BP.

Genevestigator

Q15013.

GermOnline

ENSG00000124688. Homo sapiens.

Family and domain databases

InterPro

IPR009511. MAD1/Cdc20-bound-Mad2-bd.
[Graphical view]

Pfam

PF06581. p31comet. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

35973.

Entry information

Entry name

MD2BP_HUMAN

Accession

Primary (citable) accession number: Q15013
Secondary accession number(s): B4DLV3, Q6IBB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.