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Protein

Pre-mRNA-splicing regulator WTAP

Gene

WTAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).6 Publications

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • gene expression Source: Reactome
  • mRNA methylation Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing regulator WTAP
Alternative name(s):
Female-lethal(2)D homolog
Short name:
hFL(2)D
WT1-associated protein
Wilms tumor 1-associating protein
Gene namesi
Name:WTAP
Synonyms:KIAA0105
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:16846. WTAP.

Subcellular locationi

GO - Cellular componenti

  • MIS complex Source: UniProtKB
  • nuclear membrane Source: UniProtKB
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134864847.

Polymorphism and mutation databases

BioMutaiWTAP.
DMDMi47117889.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Pre-mRNA-splicing regulator WTAPPRO_0000065983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources1 Publication
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei305 – 3051PhosphoserineCombined sources
Modified residuei306 – 3061PhosphoserineCombined sources
Modified residuei341 – 3411PhosphoserineBy similarity
Modified residuei350 – 3501PhosphothreonineBy similarity
Modified residuei388 – 3881PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ15007.
MaxQBiQ15007.
PaxDbiQ15007.
PeptideAtlasiQ15007.
PRIDEiQ15007.

PTM databases

iPTMnetiQ15007.
PhosphoSiteiQ15007.
SwissPalmiQ15007.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Inductioni

In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.

Gene expression databases

BgeeiENSG00000146457.
CleanExiHS_WTAP.
ExpressionAtlasiQ15007. baseline and differential.
GenevisibleiQ15007. HS.

Organism-specific databases

HPAiHPA010549.
HPA010550.

Interactioni

Subunit structurei

Component of the WMM complex, a N6-methyltransferase complex composed of WTAP, METTL3 and METTL14 (PubMed:24407421, PubMed:24981863, PubMed:24316715). Interacts with WT1 (PubMed:11001926, PubMed:17095724). Component of the WTAP complex composed of WTAP, ZC3H13, CBLL1, KIAA1429, RBM15, BCLAF1 and THRAP3 (PubMed:24100041).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IKZF1Q134223EBI-751647,EBI-745305
VPS52Q8N1B43EBI-751647,EBI-2799833

Protein-protein interaction databases

BioGridi114957. 22 interactions.
DIPiDIP-57185N.
IntActiQ15007. 10 interactions.
MINTiMINT-1445519.
STRINGi9606.ENSP00000351141.

Structurei

3D structure databases

ProteinModelPortaliQ15007.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fl(2)d family.Curated

Phylogenomic databases

eggNOGiKOG2991. Eukaryota.
ENOG410XNW7. LUCA.
GeneTreeiENSGT00390000013931.
HOGENOMiHOG000112606.
HOVERGENiHBG104906.
InParanoidiQ15007.
OMAiPGNGNKA.
OrthoDBiEOG091G0PNP.
PhylomeDBiQ15007.
TreeFamiTF325869.

Family and domain databases

InterProiIPR029732. WTAP.
[Graphical view]
PANTHERiPTHR15217:SF0. PTHR15217:SF0. 1 hit.
PfamiPF17098. Wtap. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15007-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNEEPLPKK VRLSETDFKV MARDELILRW KQYEAYVQAL EGKYTDLNSN
60 70 80 90 100
DVTGLRESEE KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV
110 120 130 140 150
QQPSVAQLRS TMVDPAINLF FLKMKGELEQ TKDKLEQAQN ELSAWKFTPD
160 170 180 190 200
SQTGKKLMAK CRMLIQENQE LGRQLSQGRI AQLEAELALQ KKYSEELKSS
210 220 230 240 250
QDELNDFIIQ LDEEVEGMQS TILVLQQQLK ETRQQLAQYQ QQQSQASAPS
260 270 280 290 300
TSRTTASEPV EQSEATSKDC SRLTNGPSNG SSSRQRTSGS GFHREGNTTE
310 320 330 340 350
DDFPSSPGNG NKSSNSSEER TGRGGSGYVN QLSAGYESVD SPTGSENSLT
360 370 380 390
HQSNDTDSSH DPQEEKAVSG KGNRTVGSRH VQNGLDSSVN VQGSVL
Length:396
Mass (Da):44,244
Last modified:May 10, 2004 - v2
Checksum:iB4F81B3000F77A37
GO
Isoform 2 (identifier: Q15007-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-151: S → R
     152-396: Missing.

Show »
Length:151
Mass (Da):17,801
Checksum:i70C549E73369A233
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti84 – 841E → D.
Corresponds to variant rs35059844 [ dbSNP | Ensembl ].
VAR_036854

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei151 – 1511S → R in isoform 2. 3 PublicationsVSP_010278
Alternative sequencei152 – 396245Missing in isoform 2. 3 PublicationsVSP_010279Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374416 mRNA. Translation: AAK54764.1.
D14661 mRNA. Translation: BAA03495.1.
AK127822 mRNA. Translation: BAG54579.1.
AL135914 Genomic DNA. Translation: CAI21848.1.
AL135914 Genomic DNA. Translation: CAI21849.1.
CH471051 Genomic DNA. Translation: EAW47622.1.
CH471051 Genomic DNA. Translation: EAW47624.1.
CH471051 Genomic DNA. Translation: EAW47625.1.
BC000383 mRNA. Translation: AAH00383.1.
BC004432 mRNA. Translation: AAH04432.1.
BC069192 mRNA. Translation: AAH69192.1.
AJ276706 mRNA. Translation: CAC10188.1.
AL583911 mRNA. Translation: CAC29495.1.
CCDSiCCDS5266.1. [Q15007-1]
CCDS5267.1. [Q15007-2]
RefSeqiNP_001257460.1. NM_001270531.1. [Q15007-1]
NP_001257461.1. NM_001270532.1.
NP_001257462.1. NM_001270533.1.
NP_004897.2. NM_004906.4. [Q15007-1]
NP_690596.1. NM_152857.2. [Q15007-2]
NP_690597.1. NM_152858.2. [Q15007-2]
UniGeneiHs.446091.

Genome annotation databases

EnsembliENST00000337387; ENSP00000336911; ENSG00000146457. [Q15007-2]
ENST00000358372; ENSP00000351141; ENSG00000146457. [Q15007-1]
ENST00000621533; ENSP00000479438; ENSG00000146457. [Q15007-1]
GeneIDi9589.
KEGGihsa:9589.
UCSCiuc003qsl.6. human. [Q15007-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374416 mRNA. Translation: AAK54764.1.
D14661 mRNA. Translation: BAA03495.1.
AK127822 mRNA. Translation: BAG54579.1.
AL135914 Genomic DNA. Translation: CAI21848.1.
AL135914 Genomic DNA. Translation: CAI21849.1.
CH471051 Genomic DNA. Translation: EAW47622.1.
CH471051 Genomic DNA. Translation: EAW47624.1.
CH471051 Genomic DNA. Translation: EAW47625.1.
BC000383 mRNA. Translation: AAH00383.1.
BC004432 mRNA. Translation: AAH04432.1.
BC069192 mRNA. Translation: AAH69192.1.
AJ276706 mRNA. Translation: CAC10188.1.
AL583911 mRNA. Translation: CAC29495.1.
CCDSiCCDS5266.1. [Q15007-1]
CCDS5267.1. [Q15007-2]
RefSeqiNP_001257460.1. NM_001270531.1. [Q15007-1]
NP_001257461.1. NM_001270532.1.
NP_001257462.1. NM_001270533.1.
NP_004897.2. NM_004906.4. [Q15007-1]
NP_690596.1. NM_152857.2. [Q15007-2]
NP_690597.1. NM_152858.2. [Q15007-2]
UniGeneiHs.446091.

3D structure databases

ProteinModelPortaliQ15007.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114957. 22 interactions.
DIPiDIP-57185N.
IntActiQ15007. 10 interactions.
MINTiMINT-1445519.
STRINGi9606.ENSP00000351141.

PTM databases

iPTMnetiQ15007.
PhosphoSiteiQ15007.
SwissPalmiQ15007.

Polymorphism and mutation databases

BioMutaiWTAP.
DMDMi47117889.

Proteomic databases

EPDiQ15007.
MaxQBiQ15007.
PaxDbiQ15007.
PeptideAtlasiQ15007.
PRIDEiQ15007.

Protocols and materials databases

DNASUi9589.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337387; ENSP00000336911; ENSG00000146457. [Q15007-2]
ENST00000358372; ENSP00000351141; ENSG00000146457. [Q15007-1]
ENST00000621533; ENSP00000479438; ENSG00000146457. [Q15007-1]
GeneIDi9589.
KEGGihsa:9589.
UCSCiuc003qsl.6. human. [Q15007-1]

Organism-specific databases

CTDi9589.
GeneCardsiWTAP.
HGNCiHGNC:16846. WTAP.
HPAiHPA010549.
HPA010550.
MIMi605442. gene.
neXtProtiNX_Q15007.
PharmGKBiPA134864847.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2991. Eukaryota.
ENOG410XNW7. LUCA.
GeneTreeiENSGT00390000013931.
HOGENOMiHOG000112606.
HOVERGENiHBG104906.
InParanoidiQ15007.
OMAiPGNGNKA.
OrthoDBiEOG091G0PNP.
PhylomeDBiQ15007.
TreeFamiTF325869.

Enzyme and pathway databases

ReactomeiR-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiWTAP. human.
GeneWikiiWTAP_(gene).
GenomeRNAii9589.
PROiQ15007.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000146457.
CleanExiHS_WTAP.
ExpressionAtlasiQ15007. baseline and differential.
GenevisibleiQ15007. HS.

Family and domain databases

InterProiIPR029732. WTAP.
[Graphical view]
PANTHERiPTHR15217:SF0. PTHR15217:SF0. 1 hit.
PfamiPF17098. Wtap. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFL2D_HUMAN
AccessioniPrimary (citable) accession number: Q15007
Secondary accession number(s): Q5TCL8
, Q5TCL9, Q96T28, Q9BYJ7, Q9H4E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 10, 2004
Last modified: September 7, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.