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Protein

Pre-mRNA-splicing regulator WTAP

Gene

WTAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).6 Publications

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • mRNA methylation Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW

Keywordsi

Biological processCell cycle, mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing regulator WTAP
Alternative name(s):
Female-lethal(2)D homolog
Short name:
hFL(2)D
WT1-associated protein
Wilms tumor 1-associating protein
Gene namesi
Name:WTAP
Synonyms:KIAA0105
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000146457.14
HGNCiHGNC:16846 WTAP
MIMi605442 gene
neXtProtiNX_Q15007

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9589
OpenTargetsiENSG00000146457
PharmGKBiPA134864847

Polymorphism and mutation databases

BioMutaiWTAP
DMDMi47117889

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659831 – 396Pre-mRNA-splicing regulator WTAPAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei14PhosphoserineCombined sources1
Modified residuei305PhosphoserineCombined sources1
Modified residuei306PhosphoserineCombined sources1
Modified residuei341PhosphoserineBy similarity1
Modified residuei350PhosphothreonineBy similarity1
Modified residuei388PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ15007
MaxQBiQ15007
PaxDbiQ15007
PeptideAtlasiQ15007
PRIDEiQ15007

PTM databases

iPTMnetiQ15007
PhosphoSitePlusiQ15007
SwissPalmiQ15007

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Inductioni

In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.

Gene expression databases

BgeeiENSG00000146457
CleanExiHS_WTAP
ExpressionAtlasiQ15007 baseline and differential
GenevisibleiQ15007 HS

Organism-specific databases

HPAiHPA010549
HPA010550

Interactioni

Subunit structurei

Component of the WMM complex, a N6-methyltransferase complex composed of WTAP, METTL3 and METTL14 (PubMed:24407421, PubMed:24981863, PubMed:24316715). Interacts with WT1 (PubMed:11001926, PubMed:17095724). Component of the WTAP complex composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (PubMed:24100041).6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114957, 25 interactors
CORUMiQ15007
DIPiDIP-57185N
IntActiQ15007, 21 interactors
MINTiQ15007
STRINGi9606.ENSP00000351141

Structurei

3D structure databases

ProteinModelPortaliQ15007
SMRiQ15007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fl(2)d family.Curated

Phylogenomic databases

eggNOGiKOG2991 Eukaryota
ENOG410XNW7 LUCA
GeneTreeiENSGT00390000013931
HOGENOMiHOG000112606
HOVERGENiHBG104906
InParanoidiQ15007
OMAiPGNGNKA
OrthoDBiEOG091G0PNP
PhylomeDBiQ15007
TreeFamiTF325869

Family and domain databases

InterProiView protein in InterPro
IPR033757 WTAP
IPR029732 WTAP/Mum2
PANTHERiPTHR15217 PTHR15217, 1 hit
PfamiView protein in Pfam
PF17098 Wtap, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15007-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNEEPLPKK VRLSETDFKV MARDELILRW KQYEAYVQAL EGKYTDLNSN
60 70 80 90 100
DVTGLRESEE KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV
110 120 130 140 150
QQPSVAQLRS TMVDPAINLF FLKMKGELEQ TKDKLEQAQN ELSAWKFTPD
160 170 180 190 200
SQTGKKLMAK CRMLIQENQE LGRQLSQGRI AQLEAELALQ KKYSEELKSS
210 220 230 240 250
QDELNDFIIQ LDEEVEGMQS TILVLQQQLK ETRQQLAQYQ QQQSQASAPS
260 270 280 290 300
TSRTTASEPV EQSEATSKDC SRLTNGPSNG SSSRQRTSGS GFHREGNTTE
310 320 330 340 350
DDFPSSPGNG NKSSNSSEER TGRGGSGYVN QLSAGYESVD SPTGSENSLT
360 370 380 390
HQSNDTDSSH DPQEEKAVSG KGNRTVGSRH VQNGLDSSVN VQGSVL
Length:396
Mass (Da):44,244
Last modified:May 10, 2004 - v2
Checksum:iB4F81B3000F77A37
GO
Isoform 2 (identifier: Q15007-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-151: S → R
     152-396: Missing.

Show »
Length:151
Mass (Da):17,801
Checksum:i70C549E73369A233
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03685484E → D. Corresponds to variant dbSNP:rs35059844Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010278151S → R in isoform 2. 3 Publications1
Alternative sequenceiVSP_010279152 – 396Missing in isoform 2. 3 PublicationsAdd BLAST245

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374416 mRNA Translation: AAK54764.1
D14661 mRNA Translation: BAA03495.1
AK127822 mRNA Translation: BAG54579.1
AL135914 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW47622.1
CH471051 Genomic DNA Translation: EAW47624.1
CH471051 Genomic DNA Translation: EAW47625.1
BC000383 mRNA Translation: AAH00383.1
BC004432 mRNA Translation: AAH04432.1
BC069192 mRNA Translation: AAH69192.1
AJ276706 mRNA Translation: CAC10188.1
AL583911 mRNA Translation: CAC29495.1
CCDSiCCDS5266.1 [Q15007-1]
CCDS5267.1 [Q15007-2]
RefSeqiNP_001257460.1, NM_001270531.1 [Q15007-1]
NP_001257461.1, NM_001270532.1
NP_001257462.1, NM_001270533.1
NP_004897.2, NM_004906.4 [Q15007-1]
NP_690596.1, NM_152857.2 [Q15007-2]
NP_690597.1, NM_152858.2 [Q15007-2]
UniGeneiHs.446091

Genome annotation databases

EnsembliENST00000337387; ENSP00000336911; ENSG00000146457 [Q15007-2]
ENST00000358372; ENSP00000351141; ENSG00000146457 [Q15007-1]
ENST00000621533; ENSP00000479438; ENSG00000146457 [Q15007-1]
GeneIDi9589
KEGGihsa:9589
UCSCiuc003qsl.6 human [Q15007-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiFL2D_HUMAN
AccessioniPrimary (citable) accession number: Q15007
Secondary accession number(s): Q5TCL8
, Q5TCL9, Q96T28, Q9BYJ7, Q9H4E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 10, 2004
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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