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Protein

Signal peptidase complex subunit 2

Gene

SPCS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.By similarity

GO - Molecular functioni

  1. peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. gene expression Source: Reactome
  3. regulation of insulin secretion Source: Reactome
  4. signal peptide processing Source: InterPro
  5. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  6. translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.21.89. 2681.
ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_19189. Synthesis, secretion, and deacylation of Ghrelin.
REACT_23824. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase complex subunit 2 (EC:3.4.-.-)
Alternative name(s):
Microsomal signal peptidase 25 kDa subunit
Short name:
SPase 25 kDa subunit
Gene namesi
Name:SPCS2
Synonyms:KIAA0102, SPC25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:28962. SPCS2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 8685CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei87 – 10721HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 1114LumenalSequence Analysis
Transmembranei112 – 13221HelicalSequence AnalysisAdd
BLAST
Topological domaini133 – 22694CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
  3. signal peptidase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 226225Signal peptidase complex subunit 2PRO_0000221158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine3 Publications
Modified residuei169 – 1691N6-acetyllysine1 Publication
Modified residuei191 – 1911N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15005.
PaxDbiQ15005.
PRIDEiQ15005.

PTM databases

PhosphoSiteiQ15005.

Expressioni

Gene expression databases

BgeeiQ15005.
CleanExiHS_SPC25.
HS_SPCS2.
ExpressionAtlasiQ15005. baseline and differential.
GenevestigatoriQ15005.

Organism-specific databases

HPAiHPA013386.

Interactioni

Subunit structurei

Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3.By similarity

Protein-protein interaction databases

BioGridi115133. 24 interactions.
DIPiDIP-47276N.
IntActiQ15005. 6 interactions.
MINTiMINT-1382200.
STRINGi9606.ENSP00000263672.

Structurei

3D structure databases

ProteinModelPortaliQ15005.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SPCS2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150008.
GeneTreeiENSGT00440000038181.
HOVERGENiHBG093979.
InParanoidiQ15005.
KOiK12947.
OrthoDBiEOG7X6M1N.
PhylomeDBiQ15005.
TreeFamiTF314545.

Family and domain databases

InterProiIPR009582. SigPept_cplx_su2.
[Graphical view]
PfamiPF06703. SPC25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15005-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAVQGGR SGGSGGCSGA GGASNCGTGS GRSGLLDKWK IDDKPVKIDK
60 70 80 90 100
WDGSAVKNSL DDSAKKVLLE KYKYVENFGL IDGRLTICTI SCFFAIVALI
110 120 130 140 150
WDYMHPFPES KPVLALCVIS YFVMMGILTI YTSYKEKSIF LVAHRKDPTG
160 170 180 190 200
MDPDDIWQLS SSLKRFDDKY TLKLTFISGR TKQQREAEFT KSIAKFFDHS
210 220
GTLVMDAYEP EISRLHDSLA IERKIK
Length:226
Mass (Da):25,003
Last modified:May 30, 2000 - v3
Checksum:iC1EDF687E9F7A57A
GO

Sequence cautioni

The sequence AAH08063.3 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH70276.2 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH82231.2 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA03492.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641A → V in AAH64957 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC008063 mRNA. Translation: AAH08063.3. Different initiation.
BC064957 mRNA. Translation: AAH64957.1.
BC070276 mRNA. Translation: AAH70276.2. Different initiation.
BC082231 mRNA. Translation: AAH82231.2. Different initiation.
BC106066 mRNA. Translation: AAI06067.1.
L38950 mRNA. Translation: AAA60992.1.
D14658 mRNA. Translation: BAA03492.1. Different initiation.
CR542233 mRNA. Translation: CAG47029.1.
CR542243 mRNA. Translation: CAG47039.1.
CCDSiCCDS44681.1.
RefSeqiNP_055567.2. NM_014752.2.
UniGeneiHs.282700.
Hs.719927.

Genome annotation databases

EnsembliENST00000263672; ENSP00000263672; ENSG00000118363.
GeneIDi9789.
KEGGihsa:9789.
UCSCiuc001ovu.2. human.

Polymorphism databases

DMDMi6648110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC008063 mRNA. Translation: AAH08063.3. Different initiation.
BC064957 mRNA. Translation: AAH64957.1.
BC070276 mRNA. Translation: AAH70276.2. Different initiation.
BC082231 mRNA. Translation: AAH82231.2. Different initiation.
BC106066 mRNA. Translation: AAI06067.1.
L38950 mRNA. Translation: AAA60992.1.
D14658 mRNA. Translation: BAA03492.1. Different initiation.
CR542233 mRNA. Translation: CAG47029.1.
CR542243 mRNA. Translation: CAG47039.1.
CCDSiCCDS44681.1.
RefSeqiNP_055567.2. NM_014752.2.
UniGeneiHs.282700.
Hs.719927.

3D structure databases

ProteinModelPortaliQ15005.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115133. 24 interactions.
DIPiDIP-47276N.
IntActiQ15005. 6 interactions.
MINTiMINT-1382200.
STRINGi9606.ENSP00000263672.

PTM databases

PhosphoSiteiQ15005.

Polymorphism databases

DMDMi6648110.

Proteomic databases

MaxQBiQ15005.
PaxDbiQ15005.
PRIDEiQ15005.

Protocols and materials databases

DNASUi9789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263672; ENSP00000263672; ENSG00000118363.
GeneIDi9789.
KEGGihsa:9789.
UCSCiuc001ovu.2. human.

Organism-specific databases

CTDi9789.
GeneCardsiGC11P074660.
H-InvDBHIX0028705.
HGNCiHGNC:28962. SPCS2.
HPAiHPA013386.
neXtProtiNX_Q15005.
PharmGKBiPA128394559.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG150008.
GeneTreeiENSGT00440000038181.
HOVERGENiHBG093979.
InParanoidiQ15005.
KOiK12947.
OrthoDBiEOG7X6M1N.
PhylomeDBiQ15005.
TreeFamiTF314545.

Enzyme and pathway databases

BRENDAi3.4.21.89. 2681.
ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_19189. Synthesis, secretion, and deacylation of Ghrelin.
REACT_23824. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

Miscellaneous databases

ChiTaRSiSPCS2. human.
GenomeRNAii9789.
NextBioi36862.
PROiQ15005.

Gene expression databases

BgeeiQ15005.
CleanExiHS_SPC25.
HS_SPCS2.
ExpressionAtlasiQ15005. baseline and differential.
GenevestigatoriQ15005.

Family and domain databases

InterProiIPR009582. SigPept_cplx_su2.
[Graphical view]
PfamiPF06703. SPC25. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung, Pancreas, Skin and Testis.
  2. "5'-end of human signal peptidase 25kDa subunit mRNA."
    Hartmann E.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
  3. "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1."
    Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N.
    DNA Res. 2:37-43(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-226.
    Tissue: Bone marrow.
  4. Bienvenut W.V., Lao L., Ryan K.M.
    Submitted (MAY-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-10; 174-180 AND 196-214, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Cervix carcinoma.
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 104-226.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-169 AND LYS-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSPCS2_HUMAN
AccessioniPrimary (citable) accession number: Q15005
Secondary accession number(s): Q15507
, Q3KQT0, Q641R4, Q6FG65, Q6IRX0, Q6P1P4, Q96HU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: March 4, 2015
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.