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Q15005 (SPCS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal peptidase complex subunit 2

EC=3.4.-.-
Alternative name(s):
Microsomal signal peptidase 25 kDa subunit
Short name=SPase 25 kDa subunit
Gene names
Name:SPCS2
Synonyms:KIAA0102, SPC25
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum By similarity.

Subunit structure

Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3 By similarity.

Subcellular location

Microsome membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the SPCS2 family.

Sequence caution

The sequence AAH08063.3 differs from that shown. Reason: Erroneous initiation.

The sequence AAH70276.2 differs from that shown. Reason: Erroneous initiation.

The sequence AAH82231.2 differs from that shown. Reason: Erroneous initiation.

The sequence BAA03492.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 226225Signal peptidase complex subunit 2
PRO_0000221158

Regions

Topological domain2 – 8685Cytoplasmic Potential
Transmembrane87 – 10721Helical; Potential
Topological domain108 – 1114Lumenal Potential
Transmembrane112 – 13221Helical; Potential
Topological domain133 – 22694Cytoplasmic Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.4 Ref.6 Ref.9
Modified residue1691N6-acetyllysine Ref.7
Modified residue1911N6-acetyllysine Ref.7

Experimental info

Sequence conflict641A → V in AAH64957. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q15005 [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: C1EDF687E9F7A57A

FASTA22625,003
        10         20         30         40         50         60 
MAAAAVQGGR SGGSGGCSGA GGASNCGTGS GRSGLLDKWK IDDKPVKIDK WDGSAVKNSL 

        70         80         90        100        110        120 
DDSAKKVLLE KYKYVENFGL IDGRLTICTI SCFFAIVALI WDYMHPFPES KPVLALCVIS 

       130        140        150        160        170        180 
YFVMMGILTI YTSYKEKSIF LVAHRKDPTG MDPDDIWQLS SSLKRFDDKY TLKLTFISGR 

       190        200        210        220 
TKQQREAEFT KSIAKFFDHS GTLVMDAYEP EISRLHDSLA IERKIK 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung, Pancreas, Skin and Testis.
[2]"5'-end of human signal peptidase 25kDa subunit mRNA."
Hartmann E.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
[3]"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1."
Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N.
DNA Res. 2:37-43(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-226.
Tissue: Bone marrow.
[4]Bienvenut W.V., Lao L., Ryan K.M.
Submitted (MAY-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-10; 174-180 AND 196-214, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 104-226.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-169 AND LYS-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC008063 mRNA. Translation: AAH08063.3. Different initiation.
BC064957 mRNA. Translation: AAH64957.1.
BC070276 mRNA. Translation: AAH70276.2. Different initiation.
BC082231 mRNA. Translation: AAH82231.2. Different initiation.
BC106066 mRNA. Translation: AAI06067.1.
L38950 mRNA. Translation: AAA60992.1.
D14658 mRNA. Translation: BAA03492.1. Different initiation.
CR542233 mRNA. Translation: CAG47029.1.
CR542243 mRNA. Translation: CAG47039.1.
CCDSCCDS44681.1.
RefSeqNP_055567.2. NM_014752.2.
UniGeneHs.282700.
Hs.719927.

3D structure databases

ProteinModelPortalQ15005.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115133. 13 interactions.
DIPDIP-47276N.
IntActQ15005. 5 interactions.
MINTMINT-1382200.
STRING9606.ENSP00000263672.

PTM databases

PhosphoSiteQ15005.

Polymorphism databases

DMDM6648110.

Proteomic databases

MaxQBQ15005.
PaxDbQ15005.
PRIDEQ15005.

Protocols and materials databases

DNASU9789.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263672; ENSP00000263672; ENSG00000118363.
GeneID9789.
KEGGhsa:9789.
UCSCuc001ovu.2. human.

Organism-specific databases

CTD9789.
GeneCardsGC11P074660.
H-InvDBHIX0028705.
HGNCHGNC:28962. SPCS2.
HPAHPA013386.
neXtProtNX_Q15005.
PharmGKBPA128394559.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG150008.
HOVERGENHBG093979.
InParanoidQ15005.
KOK12947.
OrthoDBEOG7X6M1N.
PhylomeDBQ15005.
TreeFamTF314545.

Enzyme and pathway databases

BRENDA3.4.21.89. 2681.
ReactomeREACT_17015. Metabolism of proteins.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ15005.
BgeeQ15005.
CleanExHS_SPC25.
HS_SPCS2.
GenevestigatorQ15005.

Family and domain databases

InterProIPR009582. SigPept_cplx_su2.
[Graphical view]
PfamPF06703. SPC25. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi9789.
NextBio36862.
PROQ15005.

Entry information

Entry nameSPCS2_HUMAN
AccessionPrimary (citable) accession number: Q15005
Secondary accession number(s): Q15507 expand/collapse secondary AC list , Q3KQT0, Q641R4, Q6FG65, Q6IRX0, Q6P1P4, Q96HU9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM