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Q15005

- SPCS2_HUMAN

UniProt

Q15005 - SPCS2_HUMAN

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Protein
Signal peptidase complex subunit 2
Gene
SPCS2, KIAA0102, SPC25
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum By similarity.

GO - Molecular functioni

  1. peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  2. cellular protein metabolic process Source: Reactome
  3. gene expression Source: Reactome
  4. regulation of insulin secretion Source: Reactome
  5. signal peptide processing Source: InterPro
  6. translation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.21.89. 2681.
ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_19189. Synthesis, secretion, and deacylation of Ghrelin.
REACT_23824. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase complex subunit 2 (EC:3.4.-.-)
Alternative name(s):
Microsomal signal peptidase 25 kDa subunit
Short name:
SPase 25 kDa subunit
Gene namesi
Name:SPCS2
Synonyms:KIAA0102, SPC25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:28962. SPCS2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 8685Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei87 – 10721Helical; Reviewed prediction
Add
BLAST
Topological domaini108 – 1114Lumenal Reviewed prediction
Transmembranei112 – 13221Helical; Reviewed prediction
Add
BLAST
Topological domaini133 – 22694Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
  3. signal peptidase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394559.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 226225Signal peptidase complex subunit 2
PRO_0000221158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine3 Publications
Modified residuei169 – 1691N6-acetyllysine1 Publication
Modified residuei191 – 1911N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15005.
PaxDbiQ15005.
PRIDEiQ15005.

PTM databases

PhosphoSiteiQ15005.

Expressioni

Gene expression databases

ArrayExpressiQ15005.
BgeeiQ15005.
CleanExiHS_SPC25.
HS_SPCS2.
GenevestigatoriQ15005.

Organism-specific databases

HPAiHPA013386.

Interactioni

Subunit structurei

Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3 By similarity.

Protein-protein interaction databases

BioGridi115133. 13 interactions.
DIPiDIP-47276N.
IntActiQ15005. 5 interactions.
MINTiMINT-1382200.
STRINGi9606.ENSP00000263672.

Structurei

3D structure databases

ProteinModelPortaliQ15005.

Family & Domainsi

Sequence similaritiesi

Belongs to the SPCS2 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150008.
HOVERGENiHBG093979.
InParanoidiQ15005.
KOiK12947.
OrthoDBiEOG7X6M1N.
PhylomeDBiQ15005.
TreeFamiTF314545.

Family and domain databases

InterProiIPR009582. SigPept_cplx_su2.
[Graphical view]
PfamiPF06703. SPC25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15005-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAAVQGGR SGGSGGCSGA GGASNCGTGS GRSGLLDKWK IDDKPVKIDK    50
WDGSAVKNSL DDSAKKVLLE KYKYVENFGL IDGRLTICTI SCFFAIVALI 100
WDYMHPFPES KPVLALCVIS YFVMMGILTI YTSYKEKSIF LVAHRKDPTG 150
MDPDDIWQLS SSLKRFDDKY TLKLTFISGR TKQQREAEFT KSIAKFFDHS 200
GTLVMDAYEP EISRLHDSLA IERKIK 226
Length:226
Mass (Da):25,003
Last modified:May 30, 2000 - v3
Checksum:iC1EDF687E9F7A57A
GO

Sequence cautioni

The sequence AAH08063.3 differs from that shown. Reason: Erroneous initiation.
The sequence AAH70276.2 differs from that shown. Reason: Erroneous initiation.
The sequence AAH82231.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAA03492.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641A → V in AAH64957. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC008063 mRNA. Translation: AAH08063.3. Different initiation.
BC064957 mRNA. Translation: AAH64957.1.
BC070276 mRNA. Translation: AAH70276.2. Different initiation.
BC082231 mRNA. Translation: AAH82231.2. Different initiation.
BC106066 mRNA. Translation: AAI06067.1.
L38950 mRNA. Translation: AAA60992.1.
D14658 mRNA. Translation: BAA03492.1. Different initiation.
CR542233 mRNA. Translation: CAG47029.1.
CR542243 mRNA. Translation: CAG47039.1.
CCDSiCCDS44681.1.
RefSeqiNP_055567.2. NM_014752.2.
UniGeneiHs.282700.
Hs.719927.

Genome annotation databases

EnsembliENST00000263672; ENSP00000263672; ENSG00000118363.
GeneIDi9789.
KEGGihsa:9789.
UCSCiuc001ovu.2. human.

Polymorphism databases

DMDMi6648110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC008063 mRNA. Translation: AAH08063.3 . Different initiation.
BC064957 mRNA. Translation: AAH64957.1 .
BC070276 mRNA. Translation: AAH70276.2 . Different initiation.
BC082231 mRNA. Translation: AAH82231.2 . Different initiation.
BC106066 mRNA. Translation: AAI06067.1 .
L38950 mRNA. Translation: AAA60992.1 .
D14658 mRNA. Translation: BAA03492.1 . Different initiation.
CR542233 mRNA. Translation: CAG47029.1 .
CR542243 mRNA. Translation: CAG47039.1 .
CCDSi CCDS44681.1.
RefSeqi NP_055567.2. NM_014752.2.
UniGenei Hs.282700.
Hs.719927.

3D structure databases

ProteinModelPortali Q15005.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115133. 13 interactions.
DIPi DIP-47276N.
IntActi Q15005. 5 interactions.
MINTi MINT-1382200.
STRINGi 9606.ENSP00000263672.

PTM databases

PhosphoSitei Q15005.

Polymorphism databases

DMDMi 6648110.

Proteomic databases

MaxQBi Q15005.
PaxDbi Q15005.
PRIDEi Q15005.

Protocols and materials databases

DNASUi 9789.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263672 ; ENSP00000263672 ; ENSG00000118363 .
GeneIDi 9789.
KEGGi hsa:9789.
UCSCi uc001ovu.2. human.

Organism-specific databases

CTDi 9789.
GeneCardsi GC11P074660.
H-InvDB HIX0028705.
HGNCi HGNC:28962. SPCS2.
HPAi HPA013386.
neXtProti NX_Q15005.
PharmGKBi PA128394559.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG150008.
HOVERGENi HBG093979.
InParanoidi Q15005.
KOi K12947.
OrthoDBi EOG7X6M1N.
PhylomeDBi Q15005.
TreeFami TF314545.

Enzyme and pathway databases

BRENDAi 3.4.21.89. 2681.
Reactomei REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_19189. Synthesis, secretion, and deacylation of Ghrelin.
REACT_23824. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

Miscellaneous databases

GenomeRNAii 9789.
NextBioi 36862.
PROi Q15005.

Gene expression databases

ArrayExpressi Q15005.
Bgeei Q15005.
CleanExi HS_SPC25.
HS_SPCS2.
Genevestigatori Q15005.

Family and domain databases

InterProi IPR009582. SigPept_cplx_su2.
[Graphical view ]
Pfami PF06703. SPC25. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung, Pancreas, Skin and Testis.
  2. "5'-end of human signal peptidase 25kDa subunit mRNA."
    Hartmann E.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
  3. "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1."
    Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N.
    DNA Res. 2:37-43(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-226.
    Tissue: Bone marrow.
  4. Bienvenut W.V., Lao L., Ryan K.M.
    Submitted (MAY-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-10; 174-180 AND 196-214, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Cervix carcinoma.
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 104-226.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-169 AND LYS-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPCS2_HUMAN
AccessioniPrimary (citable) accession number: Q15005
Secondary accession number(s): Q15507
, Q3KQT0, Q641R4, Q6FG65, Q6IRX0, Q6P1P4, Q96HU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: September 3, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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