Q15005 (SPCS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase complex subunit 2
EC=3.4.-.-

Alternative name(s):
Microsomal signal peptidase 25 kDa subunit
Short name=SPase 25 kDa subunit

Gene names

Name:	SPCS2
Synonyms:	KIAA0102, SPC25

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum By similarity.
Subunit structure	Component of the microsomal signal peptidase complex which consists of five members: SEC11A, SEC11C, SPCS1, SPCS2 and SPCS3 By similarity.
Subcellular location	Microsome membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the SPCS2 family.
Sequence caution	The sequence AAH08063.3 differs from that shown. Reason: Erroneous initiation. The sequence AAH70276.2 differs from that shown. Reason: Erroneous initiation. The sequence AAH82231.2 differs from that shown. Reason: Erroneous initiation. The sequence BAA03492.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of insulin secretion Traceable author statement. Source: Reactome signal peptide processing Inferred from electronic annotation. Source: InterPro translation Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW signal peptidase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 226	225	Signal peptidase complex subunit 2		PRO_0000221158
Regions
	Topological domain	2 – 86	85	Cytoplasmic Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Topological domain	108 – 111	4	Lumenal Potential
	Transmembrane	112 – 132	21	Helical; Potential
	Topological domain	133 – 226	94	Cytoplasmic Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.4
	Modified residue	169	1	N6-acetyllysine Ref.6
	Modified residue	191	1	N6-acetyllysine Ref.6
Experimental info
	Sequence conflict	64	1	A → V in AAH64957. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q15005 [UniParc]. Last modified May 30, 2000. Version 3. Checksum: C1EDF687E9F7A57A	FASTA	226	25,003
	10 20 30 40 50 60 MAAAAVQGGR SGGSGGCSGA GGASNCGTGS GRSGLLDKWK IDDKPVKIDK WDGSAVKNSL 70 80 90 100 110 120 DDSAKKVLLE KYKYVENFGL IDGRLTICTI SCFFAIVALI WDYMHPFPES KPVLALCVIS 130 140 150 160 170 180 YFVMMGILTI YTSYKEKSIF LVAHRKDPTG MDPDDIWQLS SSLKRFDDKY TLKLTFISGR 190 200 210 220 TKQQREAEFT KSIAKFFDHS GTLVMDAYEP EISRLHDSLA IERKIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Pancreas, Skin and Testis.
[2]	"5'-end of human signal peptidase 25kDa subunit mRNA." Hartmann E. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-37.
[3]	"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-226. Tissue: Bone marrow.
[4]	Bienvenut W.V., Lao L., Ryan K.M. Submitted (MAY-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 174-180 AND 196-214, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 104-226.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-169 AND LYS-191, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC008063 mRNA. Translation: AAH08063.3. Different initiation. BC064957 mRNA. Translation: AAH64957.1. BC070276 mRNA. Translation: AAH70276.2. Different initiation. BC082231 mRNA. Translation: AAH82231.2. Different initiation. BC106066 mRNA. Translation: AAI06067.1. L38950 mRNA. Translation: AAA60992.1. D14658 mRNA. Translation: BAA03492.1. Different initiation. CR542233 mRNA. Translation: CAG47029.1. CR542243 mRNA. Translation: CAG47039.1.
IPI	IPI00472939.
RefSeq	NP_055567.2. NM_014752.2.
UniGene	Hs.282700. Hs.719927.
3D structure databases
ProteinModelPortal	Q15005.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47276N.
IntAct	Q15005. 5 interactions.
MINT	MINT-1382200.
STRING	9606.ENSP00000263672.
PTM databases
PhosphoSite	Q15005.
Polymorphism databases
DMDM	6648110.
Proteomic databases
PaxDb	Q15005.
PRIDE	Q15005.
Protocols and materials databases
DNASU	9789.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263672; ENSP00000263672; ENSG00000118363.
GeneID	9789.
KEGG	hsa:9789.
UCSC	uc001ovu.2. human.
Organism-specific databases
CTD	9789.
GeneCards	GC11P074660.
H-InvDB	HIX0028705.
HGNC	HGNC:28962. SPCS2.
HPA	HPA013386.
neXtProt	NX_Q15005.
PharmGKB	PA128394559.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG150008.
HOVERGEN	HBG093979.
InParanoid	Q15005.
KO	K12947.
OrthoDB	EOG4RV2SD.
Enzyme and pathway databases
BRENDA	3.4.21.89. 2681.
Reactome	REACT_111217. Metabolism. REACT_116125. Disease. REACT_17015. Metabolism of proteins. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q15005.
Bgee	Q15005.
CleanEx	HS_SPC25. HS_SPCS2.
Genevestigator	Q15005.
Family and domain databases
InterPro	IPR009582. SigPept_cplx_su2. [Graphical view]
Pfam	PF06703. SPC25. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9789.
NextBio	36862.

Entry information

Entry name

SPCS2_HUMAN

Accession

Primary (citable) accession number: Q15005
Secondary accession number(s): Q15507 , Q3KQT0, Q641R4, Q6FG65, Q6IRX0, Q6P1P4, Q96HU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 30, 2000
Last modified:	May 29, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.