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Protein

Lipid A export ATP-binding/permease protein MsbA

Gene

msbA

Organism
Francisella tularensis subsp. tularensis (strain FSC 198)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi404 – 411ATPUniRule annotation8

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
Biological processLipid transport, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A export ATP-binding/permease protein MsbAUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:msbAUniRule annotation
Ordered Locus Names:FTF0109
OrganismiFrancisella tularensis subsp. tularensis (strain FSC 198)
Taxonomic identifieri393115 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei47 – 67HelicalUniRule annotationAdd BLAST21
Transmembranei88 – 108HelicalUniRule annotationAdd BLAST21
Transmembranei167 – 187HelicalUniRule annotationAdd BLAST21
Transmembranei190 – 210HelicalUniRule annotationAdd BLAST21
Transmembranei279 – 299HelicalUniRule annotationAdd BLAST21
Transmembranei305 – 325HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002716281 – 609Lipid A export ATP-binding/permease protein MsbAAdd BLAST609

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1609
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi372 – 381Combined sources10
Beta strandi384 – 394Combined sources11
Beta strandi399 – 403Combined sources5
Helixi410 – 417Combined sources8
Beta strandi424 – 430Combined sources7
Helixi435 – 437Combined sources3
Helixi440 – 445Combined sources6
Beta strandi447 – 452Combined sources6
Beta strandi458 – 460Combined sources3
Helixi461 – 466Combined sources6
Helixi475 – 484Combined sources10
Helixi488 – 492Combined sources5
Helixi497 – 499Combined sources3
Helixi504 – 506Combined sources3
Helixi511 – 525Combined sources15
Beta strandi528 – 533Combined sources6
Helixi552 – 556Combined sources5
Beta strandi558 – 563Combined sources6
Helixi567 – 572Combined sources6
Beta strandi574 – 580Combined sources7
Beta strandi583 – 588Combined sources6
Helixi590 – 595Combined sources6
Helixi599 – 605Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DGXX-ray1.73A357-609[»]
ProteinModelPortaliQ14JW6.
SMRiQ14JW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 340ABC transmembrane type-1UniRule annotationAdd BLAST295
Domaini372 – 606ABC transporterUniRule annotationAdd BLAST235

Domaini

In MsbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK11085.
OMAiSPLMEFL.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011917. ABC_transpr_lipidA.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR02203. MsbA_lipidA. 1 hit.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51239. MSBA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q14JW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANMIDKIDL KSQGSSNLSG EMTNHQKVGT LYKRLLLQVK HLWHFLLLAA
60 70 80 90 100
IGSIFFSAAD ASMIYLINPI LNYGFGPGGG ITKQSATILM LMGVGMVGLL
110 120 130 140 150
ALRSVGSFVS QYFIGSLGQK VVYKFRKDIY KRLMDLPASF FDKHSTGQII
160 170 180 190 200
SRLLYNVDQV IEATSTAIIT VVQDGTFVIG LIVVMFVSSW QLSLFLIVVG
210 220 230 240 250
PFLGLFISII NKKFRNLSRN TQSSMGNVTH TAEETIRNYK EIRIFGAQQK
260 270 280 290 300
QQNKFFKNLD YTYSQQIRTI ALDALTSPVI QIIASLVLAF SLFTIAIFGT
310 320 330 340 350
NEGDGSSWLT AGSFASFFAA AAAILKPIKN LTKVNVVIQK AVAATEDIFY
360 370 380 390 400
ILDYPAEKET GSKELAKVDG NVTIKDLSFA FGEHKVLSGV SVDIKAGQTV
410 420 430 440 450
AFVGKSGSGK TTLTSIISRF YTQHEGEILL DGVDTRELTL ENLRSHLSIV
460 470 480 490 500
SQNVHLFDDT VYNNIAFGLS REVSEEEVID ALKRANAYEF VQELSDGINT
510 520 530 540 550
NIGNNGSKLS GGQRQRISIA RALLKNAPVL IFDEATSALD NESERVVQQA
560 570 580 590 600
LESLTKSCTT IVIAHRLSTV ENADKIVVMD GGRVVESGKH QELLEQGGLY

TRLYQSGLQ
Length:609
Mass (Da):66,772
Last modified:August 22, 2006 - v1
Checksum:i996449AD1EAC7166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286280 Genomic DNA. Translation: CAL08125.1.
RefSeqiWP_003019850.1. NC_008245.1.

Genome annotation databases

EnsemblBacteriaiCAL08125; CAL08125; FTF0109.
KEGGiftf:FTF0109.

Similar proteinsi

Entry informationi

Entry nameiMSBA_FRAT1
AccessioniPrimary (citable) accession number: Q14JW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 22, 2006
Last modified: June 7, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families