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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Francisella tularensis subsp. tularensis (strain FSC 198)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Metal bindingi66MagnesiumUniRule annotation1
Metal bindingi138MagnesiumUniRule annotation1
Metal bindingi141MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:FTF0656
OrganismiFrancisella tularensis subsp. tularensis (strain FSC 198)
Taxonomic identifieri393115 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003324181 – 171Crossover junction endodeoxyribonuclease RuvCAdd BLAST171

Proteomic databases

PRIDEiQ14IH0

Structurei

3D structure databases

ProteinModelPortaliQ14IH0
SMRiQ14IH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000012181
KOiK01159

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

Q14IH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVILGIDPGS RITGFGVIKV QDNKIYYVAS GCIRITEITT PKRLKQIADG
60 70 80 90 100
ITQIINIYAP TEAAIEQIFM FQNPMGAIKL GQARGVAMCT LAINNLEVSE
110 120 130 140 150
YSAKQIKQAV VGTGGAAKSQ VQHMVQSLLG LSKKPPEDAA DALAIAICHY
160 170
HSSKSLAKIS GASRVSQKRI K
Length:171
Mass (Da):18,297
Last modified:April 29, 2008 - v2
Checksum:iB0F8E840F4C22DAD
GO

Sequence cautioni

The sequence CAL08672 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286280 Genomic DNA Translation: CAL08672.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiCAL08672; CAL08672; FTF0656
KEGGiftf:FTF0656

Similar proteinsi

Entry informationi

Entry nameiRUVC_FRAT1
AccessioniPrimary (citable) accession number: Q14IH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 73 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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