Q14HS3 (FMT_FRAT1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	FTF0925

Organism

Francisella tularensis subsp. tularensis (strain FSC 198) [Complete proteome] [HAMAP]

Taxonomic identifier

393115 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. tularensis

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the Fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000020063
Regions
	Region	113 – 116	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q14HS3 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: D8B28F8E3E107BA4	FASTA	313	35,325
	10 20 30 40 50 60 MKKLNIIFAG TPDISAQVLK DLYKSQHNIQ AVLTQPDRAK GRGKKVQFSP VKEVALANHT 70 80 90 100 110 120 PVFQPLSFKK NPEVLEQIKQ LKPDVIVVIA YGIIVPQEFL DIPRYGCLNI HVSLLPKWRG 130 140 150 160 170 180 AAPIQRAIQA GDTKTGVCIM QMDAGLDTGD ILNTLEIEIQ ETDTSQTLHD KFAKLSIKPL 190 200 210 220 230 240 LETLEKIEII KPEPQQGEPT YAHKITKQEG LIDFTKSAWQ ISCHIRAFTP WPGAYFILDD 250 260 270 280 290 300 EAIKVGEFEI LYQNTDNRKA GTIIDIYRSG FDIATSDKII RFRQLQFPNK KMLNIVDILN 310 GKDLDKYIGY KLG

References

[1]

"Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4." Chaudhuri R.R., Ren C.-P., Desmond L., Vincent G.A., Silman N.J., Brehm J.K., Elmore M.J., Hudson M.J., Forsman M., Isherwood K.E., Gurycova D., Minton N.P., Titball R.W., Pallen M.J., Vipond R. PLoS ONE 2:E352-E352(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FSC 198.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286280 Genomic DNA. Translation: CAL08941.1.
RefSeq	YP_667053.1. NC_008245.1.
3D structure databases
ProteinModelPortal	Q14HS3.
ModBase	Search...
Protein-protein interaction databases
STRING	393115.FTF0925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL08941; CAL08941; FTF0925.
GeneID	4199412.
KEGG	ftf:FTF0925.
PATRIC	17960782. VBIFraTul133500_1056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	CLSK934754.
Enzyme and pathway databases
BioCyc	FTUL393115:GJUT-943-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_FRAT1

Accession

Primary (citable) accession number: Q14HS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program