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Protein

S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Gene

queA

Organism
Francisella tularensis subsp. tularensis (strain FSC 198)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciFTUL393115:GJUT-1233-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomeraseUniRule annotation (EC:2.4.99.17UniRule annotation)
Alternative name(s):
Queuosine biosynthesis protein QueAUniRule annotation
Gene namesi
Name:queAUniRule annotation
Ordered Locus Names:FTF1215c
OrganismiFrancisella tularensis subsp. tularensis (strain FSC 198)
Taxonomic identifieri393115 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340S-adenosylmethionine:tRNA ribosyltransferase-isomerasePRO_1000015215Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ14H21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the QueA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000004402.
KOiK07568.
OMAiYGDAMFL.
OrthoDBiEOG6X6RBB.

Family and domain databases

HAMAPiMF_00113. QueA.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q14H21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFMKTDDFDY KLPEELIASY PLENRDASRL LKLNKQTGEI ADYKFTDFID
60 70 80 90 100
FINPGDLLVF NNSKVMLARL YGSKTTGAKL EYLIERIKTP KLFETHIKAN
110 120 130 140 150
RSPAIGSEIY VEDTLAKVLD KDGGMYLLEI QGDKDIYQLM EEFGHIPLPP
160 170 180 190 200
YMKRDDEEFD AERYQTVYAQ DLGSVAAPTA GLHFSKELMQ QIKDKGVDIA
210 220 230 240 250
YITLHVGSGT FKPVQVDDVE SHKMHAEVIS VPVEVCQKIR QTKENGGRVI
260 270 280 290 300
AIGTTSVRSL ETAGQNGQIE PYQGETDIFL YPGKKFNVVD AMITNFHLPK
310 320 330 340
STLIMLVSAF ADKEKIIKAY EHAIAERYRF FSYGDAMFIF
Length:340
Mass (Da):38,577
Last modified:August 22, 2006 - v1
Checksum:i37A14EED0F84FC72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286280 Genomic DNA. Translation: CAL09231.1.

Genome annotation databases

EnsemblBacteriaiCAL09231; CAL09231; FTF1215c.
KEGGiftf:FTF1215c.
PATRICi17961489. VBIFraTul133500_1407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286280 Genomic DNA. Translation: CAL09231.1.

3D structure databases

ProteinModelPortaliQ14H21.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL09231; CAL09231; FTF1215c.
KEGGiftf:FTF1215c.
PATRICi17961489. VBIFraTul133500_1407.

Phylogenomic databases

HOGENOMiHOG000004402.
KOiK07568.
OMAiYGDAMFL.
OrthoDBiEOG6X6RBB.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciFTUL393115:GJUT-1233-MONOMER.

Family and domain databases

HAMAPiMF_00113. QueA.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4."
    Chaudhuri R.R., Ren C.-P., Desmond L., Vincent G.A., Silman N.J., Brehm J.K., Elmore M.J., Hudson M.J., Forsman M., Isherwood K.E., Gurycova D., Minton N.P., Titball R.W., Pallen M.J., Vipond R.
    PLoS ONE 2:E352-E352(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FSC 198.

Entry informationi

Entry nameiQUEA_FRAT1
AccessioniPrimary (citable) accession number: Q14H21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: November 11, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.