Q14GH2 (PPNK_FRAT1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	FTF1432c

Organism

Francisella tularensis subsp. tularensis (strain FSC 198) [Complete proteome] [HAMAP]

Taxonomic identifier

393115 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. tularensis

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_1000005406

Sequences

	Sequence		Length	Mass (Da)
	Q14GH2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 7E8919F049F3C457	FASTA	296	32,506
	10 20 30 40 50 60 MTFKYHKVAI VGKHYKKEVS QMVETLYAYL QQQGLEIIIE NDTAADTSLV NVAIASLKEI 70 80 90 100 110 120 ALRCDVAIVV GGDGNFLKAS RLLALYSNIP VIGINKGKLG FLTTLAADDN ALKNDLYAIL 130 140 150 160 170 180 KGDSSVTKMS MLKYRVDNNL RAPLEASIAL NEIAITASRG LMFGLKVFID GRYAFDQRGD 190 200 210 220 230 240 GLIVSTPTGS TAHAMSAGGP ILNPNQNSVV LVPICSHSLN SRPLVISDES VIDIYITDYN 250 260 270 280 290 DPESVLSIDG RHDTILKAHQ KVTIQKARKK VTVLHTKDYN YYDTLREKLG WSKVLF

References

[1]

"Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4." Chaudhuri R.R., Ren C.-P., Desmond L., Vincent G.A., Silman N.J., Brehm J.K., Elmore M.J., Hudson M.J., Forsman M., Isherwood K.E., Gurycova D., Minton N.P., Titball R.W., Pallen M.J., Vipond R. PLoS ONE 2:E352-E352(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FSC 198.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286280 Genomic DNA. Translation: CAL09448.1.
RefSeq	YP_667505.1. NC_008245.1.
3D structure databases
ProteinModelPortal	Q14GH2.
ModBase	Search...
Protein-protein interaction databases
STRING	393115.FTF1432c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL09448; CAL09448; FTF1432c.
GeneID	4199935.
KEGG	ftf:FTF1432c.
PATRIC	17962011. VBIFraTul133500_1649.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000227221.
KO	K00858.
OMA	LLWGESP.
ProtClustDB	CLSK935030.
Enzyme and pathway databases
BioCyc	FTUL393115:GJUT-1467-MONOMER.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_FRAT1

Accession

Primary (citable) accession number: Q14GH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program