Q14FX6 (TPMT_FRAT1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	FTF1661

Organism

Francisella tularensis subsp. tularensis (strain FSC 198) [Complete proteome] [HAMAP]

Taxonomic identifier

393115 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047203
Sites
	Binding site	16	1	S-adenosyl-L-methionine By similarity
	Binding site	51	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	72	1	S-adenosyl-L-methionine By similarity
	Binding site	131	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q14FX6 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 640FFDD07F5E920C	FASTA	226	26,386
	10 20 30 40 50 60 MNKLETNNNQ YWLDRWQNDD VGFCQESPNE FLVKHFSKLN INDSSVCLIP MCGCSIDMLF 70 80 90 100 110 120 FLSKGVKVIG IELSEKAVLS FFSQNTINYE VIHGNDYKLY KGDDIEIYVA DIFNLPKIAN 130 140 150 160 170 180 NLPVFDIWYD RGAYIALPND LRTNYAKMML EVCSNNTQIL LLVMEHDKKS QTPPYSVTQA 190 200 210 220 ELIKNFSAKI KFELIDSKQR DNIPDYRKAE GMTEQYYTTY LRKKQY

References

[1]

"Genome sequencing shows that European isolates of Francisella tularensis subspecies tularensis are almost identical to US laboratory strain Schu S4." Chaudhuri R.R., Ren C.-P., Desmond L., Vincent G.A., Silman N.J., Brehm J.K., Elmore M.J., Hudson M.J., Forsman M., Isherwood K.E., Gurycova D., Minton N.P., Titball R.W., Pallen M.J., Vipond R. PLoS ONE 2:E352-E352(2007) [PubMed: 17406676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FSC 198.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286280 Genomic DNA. Translation: CAL09677.1.
RefSeq	YP_667702.1. NC_008245.1.
3D structure databases
ProteinModelPortal	Q14FX6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q14FX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4199147.
GenomeReviews	Gene locus FTF1661 in contig AM286280_GR.
KEGG	ftf:FTF1661.
PATRIC	17962576. VBIFraTul133500_1924.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG482849.
OMA	VDAIYDR.
ProtClustDB	PRK13256.
Enzyme and pathway databases
BioCyc	FTUL393115:FTF1661-MONOMER.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_FRAT1

Accession

Primary (citable) accession number: Q14FX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program