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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus HKU1 (isolate N2) (HCoV-HKU1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341NucleophileBy similarity
Active sitei299 – 2991Charge relay systemBy similarity
Active sitei302 – 3021Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2
OrganismiHuman coronavirus HKU1 (isolate N2) (HCoV-HKU1)
Taxonomic identifieri443240 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006551 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini14 – 360347Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence AnalysisAdd
BLAST
Topological domaini382 – 3854IntravirionSequence Analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313Sequence AnalysisAdd
BLAST
Chaini14 – 385372Hemagglutinin-esterasePRO_0000297762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 59By similarity
Glycosylationi83 – 831N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi107 ↔ 155By similarity
Glycosylationi110 – 1101N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi171 – 1711N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi183 ↔ 249By similarity
Disulfide bondi191 ↔ 222By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi251 – 2511N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi280 ↔ 285By similarity
Glycosylationi289 – 2891N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi317 – 3171N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi320 ↔ 344By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ14EB1.
SMRiQ14EB1. Positions 15-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 121121Esterase domain first partBy similarityAdd
BLAST
Regioni122 – 239118Receptor bindingBy similarityAdd
BLAST
Regioni240 – 352113Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q14EB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIIFLFFNF CYGFNEPLNV VSHLNHDWFL FGDSRSDCNH INNLKIKNYG
60 70 80 90 100
YLDIHPSLCN NGKISSSAGD SIFKSYHFTR FYNYTGEGDQ IIFYEGVNFN
110 120 130 140 150
PHHRFKCFFN GSNDVWIFNK VRFYRALYSN MALFRYLTFV DILYNFSFSI
160 170 180 190 200
KANICNSNIL SLNNPIFIST NYSKDVYFTL SGCSLYLVPL CLFKSNFSQY
210 220 230 240 250
YYNMDTGFAY GYSNFVSSDL DCTYISLKPG SYKIFSTGFV LSIPTKALCF
260 270 280 290 300
NKSKQFVPVQ VVDSRWNNLR ASDTSLSDAC QLPYCYFRNS SGNYVGKYDI
310 320 330 340 350
NHGDNGFTSI LSGLLYNVSC ISYYGSFLYD NFTSIWPRFS FGNCPTSAYI
360 370 380
KLNCFYDPLP IILQGILLFL ALLFIVFLLF LVYHG
Length:385
Mass (Da):44,466
Last modified:August 22, 2006 - v1
Checksum:iD015CC6DA1E9F6B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY884001 Genomic RNA. Translation: AAX76520.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY884001 Genomic RNA. Translation: AAX76520.1.

3D structure databases

ProteinModelPortaliQ14EB1.
SMRiQ14EB1. Positions 15-348.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Comparative analysis of 22 coronavirus HKU1 genomes reveals a novel genotype and evidence of natural recombination in coronavirus HKU1."
    Woo P.C.Y., Lau S.K.P., Yip C.C.Y., Huang Y., Tsoi H.-W., Chan K.-H., Yuen K.-Y.
    J. Virol. 80:7136-7145(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVHN2
AccessioniPrimary (citable) accession number: Q14EB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: August 22, 2006
Last modified: April 1, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Isolate N2 belongs to genotype B.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.