Reviewed, UniProtKB/Swiss-Prot Q14CX7 (MDM20_HUMAN)

Names and origin

Protein names

Recommended name:
    N-terminal acetyltransferase B complex subunit MDM20
      Short name=NatB complex subunit MDM20
Alternative name(s):
    Mitochondrial distribution and morphology protein 20
    p120

Gene names

Name:	MDM20
Synonyms:	C12orf30, NAP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression. Ref.6
Subunit structure	Component of the N-terminal acetyltransferase B (NatB) complex, which is composed of NAT5 and MDM20.
Subcellular location	Cytoplasm Ref.6.
Sequence similarities	Belongs to the MDM20 family. Contains 4 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat TPR repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK2	P24941	1	EBI-1048503,EBI-375096

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q14CX7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14CX7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     847-859: TISVILWVSSYCE → VSFCSLPKRHCCS
     860-972: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 972	972	N-terminal acetyltransferase B complex subunit MDM20		PRO_0000294337
Regions
	Repeat	11 – 44	34	TPR 1
	Repeat	45 – 78	34	TPR 2
	Repeat	79 – 112	34	TPR 3
	Repeat	114 – 146	33	TPR 4
	Compositional bias	871 – 877	7	Poly-Lys
Amino acid modifications
	Modified residue	691	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	847 – 859	13	TISVI…SSYCE → VSFCSLPKRHCCS in isoform 2.		VSP_026629
	Alternative sequence	860 – 972	113	Missing in isoform 2.		VSP_026630
	Natural variant	426	1	L → F: dbSNP rs16941860.		VAR_054099
	Natural variant	789	1	S → R in a breast cancer sample; somatic mutation. Ref.7		VAR_035872
	Natural variant	876	1	K → R: dbSNP rs12231744.		VAR_033156
	Natural variant	915	1	L → I: dbSNP rs12298022.		VAR_054100
Experimental info
	Sequence conflict	202	1	I → T in BAB14432. Ref.4
	Sequence conflict	466	1	Y → S in BAB14432. Ref.4
	Sequence conflict	672	1	S → T in BAB14432. Ref.4
	Sequence conflict	817	1	S → G in CAE46062. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 232B8FD14447DDD6	FASTA	972	112,292
	10 20 30 40 50 60 MATRGHVQDP NDRRLRPIYD YLDNGNNKMA IQQADKLLKK HKDLHCAKVL KAIGLQRTGK 70 80 90 100 110 120 QEEAFTLAQE VAALEPTDDN SLQALTILYR EMHRPELVTK LYEAAVKKVP NSEEYHSHLF 130 140 150 160 170 180 MAYARVGEYK KMQQAGMALY KIVPKNPYYF WSVMSLIMQS ISAQDENLSK TMFLPLAERM 190 200 210 220 230 240 VEKMVKEDKI EAEAEVELYY MILERLGKYQ EALDVIRGKL GEKLTSEIQS RENKCMAMYK 250 260 270 280 290 300 KLSRWPECNA LSRRLLLKNS DDWQFYLTYF DSVFRLIEEA WSPPAEGEHS LEGEVHYSAE 310 320 330 340 350 360 KAVKFIEDRI TEESKSSRHL RGPHLAKLEL IRRLRSQGCN DEYKLGDPEE LMFQYFKKFG 370 380 390 400 410 420 DKPCCFTDLK VFVDLLPATQ CTKFINQLLG VVPLSTPTED KLALPADIRA LQQHLCVVQL 430 440 450 460 470 480 TRLLGLYHTM DKNQKLSVVR ELMLRYQHGL EFGKTCLKTE LQFSDYYCLL AVHALIDVWR 490 500 510 520 530 540 ETGDETTVWQ ALTLLEEGLT HSPSNAQFKL LLVRIYCMLG AFEPVVDLYS SLDAKHIQHD 550 560 570 580 590 600 TIGYLLTRYA ESLGQYAAAS QSCNFALRFF HSNQKDTSEY IIQAYKYGAF EKIPEFIAFR 610 620 630 640 650 660 NRLNNSLHFA QVRTERMLLD LLLEANISTS LAESIKSMNL RPEEDDIPWE DLRDNRDLNV 670 680 690 700 710 720 FFSWDPKDRD VSEEHKKLSL EEETLWLRIR SLTLRLISGL PSLNHPVEPK NSEKTAENGV 730 740 750 760 770 780 SSRIDILRLL LQQLEATLET GKRFIEKDIQ YPFLGPVPTR MGGFFNSGCS QCQISSFYLV 790 800 810 820 830 840 NDIYELDTSG LEDTMEIQER IENSFKSLLD QLKDVFSKCK GDLLEVKDGN LKTHPTLLEN 850 860 870 880 890 900 LVFFVETISV ILWVSSYCES VLRPYKLNLQ KKKKKKKETS IIMPPVFTSF QDYVTGLQTL 910 920 930 940 950 960 ISNVVDHIKG LETHLIALKL EELILEDTSL SPEERKFSKT VQGKVQSSYL HSLLEMGELL 970 KKRLETTKKL KI
	Isoform 2. Checksum: 5918C0A1EB63A438	FASTA	859	99,269
	10 20 30 40 50 60 MATRGHVQDP NDRRLRPIYD YLDNGNNKMA IQQADKLLKK HKDLHCAKVL KAIGLQRTGK 70 80 90 100 110 120 QEEAFTLAQE VAALEPTDDN SLQALTILYR EMHRPELVTK LYEAAVKKVP NSEEYHSHLF 130 140 150 160 170 180 MAYARVGEYK KMQQAGMALY KIVPKNPYYF WSVMSLIMQS ISAQDENLSK TMFLPLAERM 190 200 210 220 230 240 VEKMVKEDKI EAEAEVELYY MILERLGKYQ EALDVIRGKL GEKLTSEIQS RENKCMAMYK 250 260 270 280 290 300 KLSRWPECNA LSRRLLLKNS DDWQFYLTYF DSVFRLIEEA WSPPAEGEHS LEGEVHYSAE 310 320 330 340 350 360 KAVKFIEDRI TEESKSSRHL RGPHLAKLEL IRRLRSQGCN DEYKLGDPEE LMFQYFKKFG 370 380 390 400 410 420 DKPCCFTDLK VFVDLLPATQ CTKFINQLLG VVPLSTPTED KLALPADIRA LQQHLCVVQL 430 440 450 460 470 480 TRLLGLYHTM DKNQKLSVVR ELMLRYQHGL EFGKTCLKTE LQFSDYYCLL AVHALIDVWR 490 500 510 520 530 540 ETGDETTVWQ ALTLLEEGLT HSPSNAQFKL LLVRIYCMLG AFEPVVDLYS SLDAKHIQHD 550 560 570 580 590 600 TIGYLLTRYA ESLGQYAAAS QSCNFALRFF HSNQKDTSEY IIQAYKYGAF EKIPEFIAFR 610 620 630 640 650 660 NRLNNSLHFA QVRTERMLLD LLLEANISTS LAESIKSMNL RPEEDDIPWE DLRDNRDLNV 670 680 690 700 710 720 FFSWDPKDRD VSEEHKKLSL EEETLWLRIR SLTLRLISGL PSLNHPVEPK NSEKTAENGV 730 740 750 760 770 780 SSRIDILRLL LQQLEATLET GKRFIEKDIQ YPFLGPVPTR MGGFFNSGCS QCQISSFYLV 790 800 810 820 830 840 NDIYELDTSG LEDTMEIQER IENSFKSLLD QLKDVFSKCK GDLLEVKDGN LKTHPTLLEN 850 LVFFVEVSFC SLPKRHCCS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"P120 which associates with nascent polypeptide chain." Hotokezaka H., Wiedmann M. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterine endothelium.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 100-972.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Identification of the human N(alpha)-acetyltransferase complex B (hNatB): a complex important for cell-cycle progression." Starheim K.K., Arnesen T., Gromyko D., Ryningen A., Varhaug J.E., Lillehaug J.R. Biochem. J. 415:325-331(2008) [PubMed: 18570629] [Abstract] Cited for: FUNCTION, INTERACTION WITH NAT5, SUBCELLULAR LOCATION.
[7]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-789.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB054990 mRNA. Translation: BAC80174.1. BX641140 mRNA. Translation: CAE46062.1. Different initiation. BC034357 mRNA. Translation: AAH34357.1. BC113585 mRNA. Translation: AAI13586.1. BC113587 mRNA. Translation: AAI13588.1. AK023151 mRNA. Translation: BAB14432.1. Different initiation.
IPI	IPI00025890. IPI00852843.
RefSeq	NP_079229.2.
UniGene	Hs.530941
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14CX7. 1 interaction.
STRING	Q14CX7.
Proteomic databases
PRIDE	Q14CX7.
Genome annotation databases
Ensembl	ENST00000261745; ENSP00000261745; ENSG00000111300; Homo sapiens. [Genome view]
GeneID	80018.
KEGG	hsa:80018.
UCSC	uc001ttm.1. human. uc009zvz.1. human.
Organism-specific databases
CTD	80018.
GeneCards	GC12M110948.
HGNC	HGNC:25783. C12orf30.
MIM	612755. gene.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09198.
HOVERGEN	Q14CX7.
InParanoid	Q14CX7.
OMA	DTIGYLL.
OrthoDB	EOG97M4HW.
PhylomeDB	Q14CX7.
Gene expression databases
ArrayExpress	Q14CX7.
Bgee	Q14CX7.
CleanEx	HS_C12orf30.
Genevestigator	Q14CX7.
Family and domain databases
InterPro	IPR019183. N-acetylTrfase_B_cplx_non-cat. IPR013026. TPR-contain. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF09797. NatB_MDM20. 1 hit. [Graphical view]
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	70134.
SOURCE	Search...

Entry information

Entry name

MDM20_HUMAN

Accession

Primary (citable) accession number: Q14CX7
Secondary accession number(s): A0JLU7 , Q6MZH1, Q7Z4N6, Q9H911

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	August 22, 2006
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.