Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q14CN2 (CLCA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-activated chloride channel regulator 4
Alternative name(s):
Calcium-activated chloride channel family member 4
Short name=hCLCA4
Calcium-activated chloride channel protein 2
Short name=CaCC-2
Short name=hCaCC-2
Gene names
Name:CLCA4
Synonyms:CaCC2
ORF Names:UNQ562/PRO1124
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in mediating calcium-activated chloride conductance.

Subcellular location

Cell membrane; Single-pass membrane protein By similarity. Apical cell membrane By similarity. Secreted By similarity. Note: The C-terminus 30 kDa form is anchored to the membrane By similarity. The N-terminus 110 kDa form is released from the membrane triggered by an unknown stimulus By similarity.

Tissue specificity

Primarily expressed in the digestive tract, mainly in colon. Detected in smaller amounts in brain, urogenital organs, testis, and salivary and mammary glands. Highly expressed in the epithelial layer and submucosal gland of the inferior turbinate mucosa. Lower levels in the epithelial layer of nasal polyp. Ref.1 Ref.5 Ref.6

Induction

Down-regulated in oral tongue squamous cell carcinomas. Ref.8

Post-translational modification

Processed at a monobasic residue to yield a larger N-terminal and a smaller C-terminal cleavage products of 90 to 110 and 30 to 35 kDa, respectively By similarity.

Sequence similarities

Belongs to the CLCR family.

Contains 1 VWFA domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 919898Calcium-activated chloride channel regulator 4
PRO_0000345389
Chain22 – ?Calcium-activated chloride channel regulator 4, 110 kDa formPRO_0000345390
Chain? – 919Calcium-activated chloride channel regulator 4, 30 kDa formPRO_0000345391

Regions

Transmembrane895 – 91521Helical; Potential
Domain306 – 476171VWFA

Amino acid modifications

Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation5421N-linked (GlcNAc...) Potential
Glycosylation5881N-linked (GlcNAc...) Potential
Glycosylation6281N-linked (GlcNAc...) Potential
Glycosylation8111N-linked (GlcNAc...) Ref.7
Glycosylation8321N-linked (GlcNAc...) Potential
Glycosylation8371N-linked (GlcNAc...) Potential
Glycosylation8521N-linked (GlcNAc...) Potential

Natural variations

Natural variant431P → S.
Corresponds to variant rs2231580 [ dbSNP | Ensembl ].
VAR_045816
Natural variant4431D → V.
Corresponds to variant rs2839932 [ dbSNP | Ensembl ].
VAR_045817
Natural variant4491M → L.
Corresponds to variant rs1011048 [ dbSNP | Ensembl ].
VAR_045818
Natural variant8101V → L. Ref.4
Corresponds to variant rs2231604 [ dbSNP | Ensembl ].
VAR_045819
Natural variant877 – 8782Missing.
VAR_045820

Experimental info

Sequence conflict3031S → R in AAD48398. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q14CN2 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: 47C0DF125A319810

FASTA919101,283
        10         20         30         40         50         60 
MGLFRGFVFL LVLCLLHQSN TSFIKLNNNG FEDIVIVIDP SVPEDEKIIE QIEDMVTTAS 

        70         80         90        100        110        120 
TYLFEATEKR FFFKNVSILI PENWKENPQY KRPKHENHKH ADVIVAPPTL PGRDEPYTKQ 

       130        140        150        160        170        180 
FTECGEKGEY IHFTPDLLLG KKQNEYGPPG KLFVHEWAHL RWGVFDEYNE DQPFYRAKSK 

       190        200        210        220        230        240 
KIEATRCSAG ISGRNRVYKC QGGSCLSRAC RIDSTTKLYG KDCQFFPDKV QTEKASIMFM 

       250        260        270        280        290        300 
QSIDSVVEFC NEKTHNQEAP SLQNIKCNFR STWEVISNSE DFKNTIPMVT PPPPPVFSLL 

       310        320        330        340        350        360 
KISQRIVCLV LDKSGSMGGK DRLNRMNQAA KHFLLQTVEN GSWVGMVHFD STATIVNKLI 

       370        380        390        400        410        420 
QIKSSDERNT LMAGLPTYPL GGTSICSGIK YAFQVIGELH SQLDGSEVLL LTDGEDNTAS 

       430        440        450        460        470        480 
SCIDEVKQSG AIVHFIALGR AADEAVIEMS KITGGSHFYV SDEAQNNGLI DAFGALTSGN 

       490        500        510        520        530        540 
TDLSQKSLQL ESKGLTLNSN AWMNDTVIID STVGKDTFFL ITWNSLPPSI SLWDPSGTIM 

       550        560        570        580        590        600 
ENFTVDATSK MAYLSIPGTA KVGTWAYNLQ AKANPETLTI TVTSRAANSS VPPITVNAKM 

       610        620        630        640        650        660 
NKDVNSFPSP MIVYAEILQG YVPVLGANVT AFIESQNGHT EVLELLDNGA GADSFKNDGV 

       670        680        690        700        710        720 
YSRYFTAYTE NGRYSLKVRA HGGANTARLK LRPPLNRAAY IPGWVVNGEI EANPPRPEID 

       730        740        750        760        770        780 
EDTQTTLEDF SRTASGGAFV VSQVPSLPLP DQYPPSQITD LDATVHEDKI ILTWTAPGDN 

       790        800        810        820        830        840 
FDVGKVQRYI IRISASILDL RDSFDDALQV NTTDLSPKEA NSKESFAFKP ENISEENATH 

       850        860        870        880        890        900 
IFIAIKSIDK SNLTSKVSNI AQVTLFIPQA NPDDIDPTPT PTPTPTPDKS HNSGVNISTL 

       910 
VLSVIGSVVI VNFILSTTI 

« Hide

References

« Hide 'large scale' references
[1]"Identification of three novel members of the calcium-dependent chloride channel (CaCC) family predominantly expressed in the digestive tract and trachea."
Agnel M., Vermat T., Culouscou J.-M.
FEBS Lett. 455:295-301(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT 877-PRO-THR-878 DEL, TISSUE SPECIFICITY.
Tissue: Colon.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-810 AND 877-PRO-THR-878 DEL.
[5]"The CLCA gene locus as a modulator of the gastrointestinal basic defect in cystic fibrosis."
Ritzka M., Stanke F., Jansen S., Gruber A.D., Pusch L., Woelfl S., Veeze H.J., Halley D.J., Tummler B.
Hum. Genet. 115:483-491(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps."
Lee S.H., Park J.H., Jung H.H., Lee S.H., Oh J.W., Lee H.M., Jun H.S., Cho W.J., Lee J.Y.
Acta Oto-Laryngol. 125:745-752(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-811.
Tissue: Saliva.
[8]"Transcriptomic dissection of tongue squamous cell carcinoma."
Ye H., Yu T., Temam S., Ziober B.L., Wang J., Schwartz J.L., Mao L., Wong D.T., Zhou X.
BMC Genomics 9:69-69(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF127035 mRNA. Translation: AAD48398.1.
AY358470 mRNA. Translation: AAQ88834.1.
AL122002 Genomic DNA. Translation: CAI22170.1.
BC113687 mRNA. Translation: AAI13688.1.
BC113689 mRNA. Translation: AAI13690.1.
RefSeqNP_036260.2. NM_012128.3.
UniGeneHs.567422.

3D structure databases

ProteinModelPortalQ14CN2.
SMRQ14CN2. Positions 303-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000359594.

Chemistry

ChEMBLCHEMBL2364708.

Protein family/group databases

MEROPSM87.002.
TCDB1.A.13.1.2. the epithelial chloride channel (e-clc) family.

Polymorphism databases

DMDM205831469.

Proteomic databases

PaxDbQ14CN2.
PRIDEQ14CN2.

Protocols and materials databases

DNASU22802.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370563; ENSP00000359594; ENSG00000016602.
GeneID22802.
KEGGhsa:22802.
UCSCuc009wcs.3. human.

Organism-specific databases

CTD22802.
GeneCardsGC01P087012.
H-InvDBHIX0000752.
HGNCHGNC:2018. CLCA4.
HPAHPA017045.
neXtProtNX_Q14CN2.
Orphanet586. Cystic fibrosis.
PharmGKBPA26545.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42884.
HOGENOMHOG000015107.
HOVERGENHBG005560.
KOK05030.
OMAPWMNDTV.
OrthoDBEOG78WKR1.
PhylomeDBQ14CN2.
TreeFamTF328396.

Gene expression databases

ArrayExpressQ14CN2.
BgeeQ14CN2.
CleanExHS_CLCA4.
GenevestigatorQ14CN2.

Family and domain databases

Gene3D3.40.50.410. 1 hit.
InterProIPR004727. CaCC_prot.
IPR013642. Cl_channel_Ca.
IPR015394. DUF1973.
IPR002035. VWF_A.
[Graphical view]
PfamPF08434. CLCA_N. 1 hit.
PF09315. DUF1973. 1 hit.
[Graphical view]
SMARTSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
TIGRFAMsTIGR00868. hCaCC. 1 hit.
PROSITEPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCLCA4.
GenomeRNAi22802.
NextBio43156.
PROQ14CN2.

Entry information

Entry nameCLCA4_HUMAN
AccessionPrimary (citable) accession number: Q14CN2
Secondary accession number(s): Q6UX81, Q9UNF7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: April 16, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM