Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-1,4-N-acetylglucosaminyltransferase

Gene

A4gnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. Necessary for the synthesis of type III mucin which is specifically produced in the stomach, duodenum, and pancreatic duct. May protect against inflammation-associated gastric adenocarcinoma.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • acetylglucosaminyltransferase activity Source: MGI

GO - Biological processi

  • glycoprotein biosynthetic process Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • protein O-linked glycosylation Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,4-N-acetylglucosaminyltransferase1 Publication (EC:2.4.1.-Curated)
Short name:
Alpha4GnT1 Publication
Gene namesi
Name:A4gntImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2143261. A4gnt.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicCurated4
Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini26 – 341LumenalCuratedAdd BLAST316

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Viable with no gross defects. Gastric mucosa cells and duodenal Brunner's glands have an altered O-linked glycosylation profile with complete loss of terminal alpha-1,4-linked N-acetylglucosamine residues (alpha-GlcNAc). Animals develop spontaneous gastric adenocarcinomas, with all individuals affected by 60 weeks of age. Hyperplasia of surface mucous cells and pyloric gland cells is observed as early as 5 weeks of age, associated with increased inflammatory responses in the gastric mucosa.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004398551 – 341Alpha-1,4-N-acetylglucosaminyltransferaseAdd BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi100N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ14BT6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037953.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045629.

Structurei

3D structure databases

SMRiQ14BT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi168 – 170DXD motifBy similarity3

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 32 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
KOiK08251.
OMAiFLNPQRF.
OrthoDBiEOG091G08S2.
TreeFamiTF324053.

Family and domain databases

InterProiView protein in InterPro
IPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q14BT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKEIYLSLS LVLVFACGLL YQLTMRSQCF FACLPPFSFP QGLDGLLRSG
60 70 80 90 100
RSIMFIETSE RVEPPPMVSC AVESAAKIYP EQPIIFFMKG LRDSVQLTSN
110 120 130 140 150
TSYPAFSLLS AINNVFFVPL DMERLFKDTP LFSWYTKVNS STEKHWLHVS
160 170 180 190 200
SDAARLAIIW KYGGIYMDTD VISLQPIPEE NFLAAQGSRH SSNGVFGFLP
210 220 230 240 250
HHPFLWACME NFVEHYDSTI WGNQGPQLMT RMLRVWCRLK DFHGLGDLKC
260 270 280 290 300
LNISFLHPQR FYPIPYPQWK RYYQVWDKEP SFNESYALHL WNYMNKEGKT
310 320 330 340
VVRGSKTLVE NLYQKHCPKT YRVLIQGAEG TVSKKPGTGS R
Length:341
Mass (Da):39,276
Last modified:August 22, 2006 - v1
Checksum:i17733C8FAFC37A44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC156497 Genomic DNA. No translation available.
AC157949 Genomic DNA. No translation available.
CH466560 Genomic DNA. Translation: EDL21015.1.
BC115614 mRNA. Translation: AAI15615.1.
BC115615 mRNA. Translation: AAI15616.1.
RefSeqiNP_001070892.1. NM_001077424.2.
XP_006511309.1. XM_006511246.3.
UniGeneiMm.297753.

Genome annotation databases

EnsembliENSMUST00000042553; ENSMUSP00000045629; ENSMUSG00000037953.
GeneIDi333424.
KEGGimmu:333424.
UCSCiuc009rej.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC156497 Genomic DNA. No translation available.
AC157949 Genomic DNA. No translation available.
CH466560 Genomic DNA. Translation: EDL21015.1.
BC115614 mRNA. Translation: AAI15615.1.
BC115615 mRNA. Translation: AAI15616.1.
RefSeqiNP_001070892.1. NM_001077424.2.
XP_006511309.1. XM_006511246.3.
UniGeneiMm.297753.

3D structure databases

SMRiQ14BT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045629.

Protein family/group databases

CAZyiGT32. Glycosyltransferase Family 32.

Proteomic databases

MaxQBiQ14BT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042553; ENSMUSP00000045629; ENSMUSG00000037953.
GeneIDi333424.
KEGGimmu:333424.
UCSCiuc009rej.2. mouse.

Organism-specific databases

CTDi51146.
MGIiMGI:2143261. A4gnt.

Phylogenomic databases

eggNOGiKOG1928. Eukaryota.
ENOG4111PD8. LUCA.
GeneTreeiENSGT00510000047981.
HOGENOMiHOG000254772.
HOVERGENiHBG106704.
KOiK08251.
OMAiFLNPQRF.
OrthoDBiEOG091G08S2.
TreeFamiTF324053.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037953.

Family and domain databases

InterProiView protein in InterPro
IPR007652. A1-4-GlycosylTfrase_dom.
IPR007577. GlycoTrfase_DXD_sugar-bd_CS.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF04572. Gb3_synth. 1 hit.
PF04488. Gly_transf_sug. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA4GCT_MOUSE
AccessioniPrimary (citable) accession number: Q14BT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
Last sequence update: August 22, 2006
Last modified: May 10, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.