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Q14BI7

- TDRD9_MOUSE

UniProt

Q14BI7 - TDRD9_MOUSE

Protein

Putative ATP-dependent RNA helicase TDRD9

Gene

Tdrd9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 3 (02 Mar 2010)
      Previous versions | rss
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    Functioni

    Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi157 – 1648ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. nucleic acid binding Source: InterPro
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. DNA methylation involved in gamete generation Source: UniProtKB
    3. fertilization Source: UniProtKB
    4. gene silencing by RNA Source: UniProtKB-KW
    5. male meiosis Source: UniProtKB
    6. multicellular organismal development Source: UniProtKB-KW
    7. piRNA metabolic process Source: UniProtKB
    8. spermatogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Helicase, Hydrolase

    Keywords - Biological processi

    Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ATP-dependent RNA helicase TDRD9 (EC:3.6.4.13)
    Alternative name(s):
    Tudor domain-containing protein 9
    Gene namesi
    Name:Tdrd9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1921941. Tdrd9.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. piP-body Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable but show male sterility with chromosome synapsis failure. In fetal testes, LINE-1 (L1) transposable elements derepression and an aberrant piRNA profile in prospermatogonia, followed by cognate DNA demethylation are observed.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13831383Putative ATP-dependent RNA helicase TDRD9PRO_0000333814Add
    BLAST

    Proteomic databases

    PaxDbiQ14BI7.
    PRIDEiQ14BI7.

    PTM databases

    PhosphoSiteiQ14BI7.

    Expressioni

    Tissue specificityi

    Predominantly expressed in reproductive organs. Detected in mitotic spermatogonia, meiotic spermatocytes (predominantly at the pachytene stage), haploid spermatids in the testis, and in growing oocytes in the ovary (at protein level).1 Publication

    Gene expression databases

    BgeeiQ14BI7.
    GenevestigatoriQ14BI7.

    Interactioni

    Subunit structurei

    Interacts with piRNA-associated proteins PIWIL1 and PIWIL4.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ14BI7.
    SMRiQ14BI7. Positions 126-720, 918-1082.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini144 – 310167Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini378 – 545168Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini945 – 100561TudorPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi256 – 2594DEAH box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 Tudor domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    GeneTreeiENSGT00740000115350.
    HOGENOMiHOG000047965.
    HOVERGENiHBG072266.
    InParanoidiB1Q3J8.
    OMAiINDWFTI.
    OrthoDBiEOG7FV3PF.
    PhylomeDBiQ14BI7.
    TreeFamiTF324869.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR002999. Tudor.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00567. TUDOR. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00333. TUDOR. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50304. TUDOR. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q14BI7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLRKLTVDQI NDWFTIGKTV TNVELLGLPP AFPAEAPREE VQRSEEVPNE     50
    DPTAQAQVPV KATAPARPAS TSGRSLSQRS SEMEYINKYR QLEEQELDIY 100
    GQDQPPSGPG LRSPLAKLSN VACIPETTYK YPDLPINRCK EEVISLIESN 150
    SVVIIHGATG SGKSTQLPQY VLDHYTQRSA FCNIVVTQPR KIGASSIARW 200
    ISKERSWTLG GLVGYQVGLE KIATEDTRLI YMTTGVLLQK IVSAKSLMEF 250
    THIFIDEVHE RTEEMDFLLL VVRKLLRTNS RFVKVVLMSA TINCKQFADY 300
    FAVPVQNKMN PAYVFEVEGK PHAIEEYYLN DLGHIYHSGL PYRLEEPVIT 350
    KDVYEVAVSL IQMFDDLDMK ESGNKTWSGA QFVSERSSVL VFLPGLGEIN 400
    YMHELLTNMI HKRLQVYPLH SSVTLEEQNN VFLSPVPGYR KIILSTNIAE 450
    SSVTVPDVKY VIDFCLTRTL VCDEDTNYQS LRLSWASKTS CDQRKGRAGR 500
    VSKGYCYRLI PRDFWDSAIP DHVVPEMLRC PLGSTILKVK LLDMGEPRAL 550
    LATALSPPSL SDIERTILLL KEVGALAVSG QREDENPHDG ELTFLGRVLA 600
    QLPVSQQLGK LVVLGHVFGC LDECLIIAAA LSLKNFFTMP FRQHLDGYRN 650
    KVHFSGSSRS DCLALVEAFR AWQACRQRGE LRRPKDELDW GRLNYIQIKR 700
    IREVAELYEE LKNRISQFNM FVGPHHPVLD QEYPYKQRFI LQVVLAGAFY 750
    PNYFTFGQPD EEMAVRELAG KDPKTTVVLK HIPPYGFLYY KQLQSLFRQC 800
    GQVKSIVFDG AKAFVEFSRN PTERFKTLPA VNLAVKMSQL KVSLELSVHA 850
    AEEIEGKVQG GSVSKLRNTR VNVDFQKQTV DPMQVSFNTL DRPRTVADLL 900
    LTIDVTEVVE VGHFWGYRID ERNAELLKQL TAEINRLELV PLPIHPHPDL 950
    VCLAPFTDYN KESYFRAQIL YVSGNSAEVF FVDYGNRSHV DLDLLREIPC 1000
    QFLELPFQAL EFKICKMRPS AKSLICGEHW SGGAHGRFAA LVGGCPLLVK 1050
    VFSIVHSVLH VDVYRYSGAQ DAVNVRDVLI REGYAELAEE SYESKQSYEV 1100
    LKGFFAKSVD TMPDGSVSSP LKDDEKHLLR ILLESFASNR LGAPNCKAVL 1150
    HGPFNPYELK CHSLTRISKF RCVWIEKESI NSVVISDSPA DLHQRMLVAA 1200
    SLSVNETGST MLLRETSLMP HIPGLPALLS MLFAPVMELR VDREGKCYTG 1250
    VLCGLGWNSA TEAPILPEHD IELAFDVRLN VEDIVEINIL RAAINKLVCD 1300
    GPNGSKYLGP ERIAQLQENA RQKLLGLFCR LKPREKITPQ WHEKPYEWNQ 1350
    VDPRLIMEQA EPEGSPGKST SLYQLHTPVV LSP 1383
    Length:1,383
    Mass (Da):155,980
    Last modified:March 2, 2010 - v3
    Checksum:i55946F4F639B5777
    GO
    Isoform 2 (identifier: Q14BI7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-401: Missing.

    Show »
    Length:982
    Mass (Da):110,899
    Checksum:i0F9C78208DD4792F
    GO
    Isoform 3 (identifier: Q14BI7-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-772: Missing.
         773-813: PKTTVVLKHI...KSIVFDGAKA → MDIGTKCTSQ...SEESCGVPRA

    Note: No experimental confirmation available.

    Show »
    Length:611
    Mass (Da):68,306
    Checksum:i43784340033630A0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 772772Missing in isoform 3. 1 PublicationVSP_033553Add
    BLAST
    Alternative sequencei1 – 401401Missing in isoform 2. 1 PublicationVSP_033554Add
    BLAST
    Alternative sequencei773 – 81341PKTTV…DGAKA → MDIGTKCTSQVAAGVTAWHL WRRSGPGRLADSEESCGVPR A in isoform 3. 1 PublicationVSP_033555Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB362563 mRNA. Translation: BAG15992.1.
    AC112520 Genomic DNA. No translation available.
    AC132623 Genomic DNA. No translation available.
    BC115831 mRNA. Translation: AAI15832.1.
    BC116656 mRNA. Translation: AAI16657.1.
    CCDSiCCDS49184.1. [Q14BI7-1]
    RefSeqiNP_083332.1. NM_029056.1. [Q14BI7-1]
    XP_006516384.1. XM_006516321.1. [Q14BI7-2]
    UniGeneiMm.60648.

    Genome annotation databases

    EnsembliENSMUST00000079009; ENSMUSP00000078022; ENSMUSG00000054003. [Q14BI7-1]
    GeneIDi74691.
    KEGGimmu:74691.
    UCSCiuc007pei.1. mouse. [Q14BI7-1]
    uc007pej.1. mouse. [Q14BI7-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB362563 mRNA. Translation: BAG15992.1 .
    AC112520 Genomic DNA. No translation available.
    AC132623 Genomic DNA. No translation available.
    BC115831 mRNA. Translation: AAI15832.1 .
    BC116656 mRNA. Translation: AAI16657.1 .
    CCDSi CCDS49184.1. [Q14BI7-1 ]
    RefSeqi NP_083332.1. NM_029056.1. [Q14BI7-1 ]
    XP_006516384.1. XM_006516321.1. [Q14BI7-2 ]
    UniGenei Mm.60648.

    3D structure databases

    ProteinModelPortali Q14BI7.
    SMRi Q14BI7. Positions 126-720, 918-1082.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q14BI7.

    Proteomic databases

    PaxDbi Q14BI7.
    PRIDEi Q14BI7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000079009 ; ENSMUSP00000078022 ; ENSMUSG00000054003 . [Q14BI7-1 ]
    GeneIDi 74691.
    KEGGi mmu:74691.
    UCSCi uc007pei.1. mouse. [Q14BI7-1 ]
    uc007pej.1. mouse. [Q14BI7-3 ]

    Organism-specific databases

    CTDi 122402.
    MGIi MGI:1921941. Tdrd9.

    Phylogenomic databases

    eggNOGi COG1643.
    GeneTreei ENSGT00740000115350.
    HOGENOMi HOG000047965.
    HOVERGENi HBG072266.
    InParanoidi B1Q3J8.
    OMAi INDWFTI.
    OrthoDBi EOG7FV3PF.
    PhylomeDBi Q14BI7.
    TreeFami TF324869.

    Miscellaneous databases

    NextBioi 341402.
    PROi Q14BI7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q14BI7.
    Genevestigatori Q14BI7.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR002999. Tudor.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00567. TUDOR. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00333. TUDOR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50304. TUDOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of retroelement expression and genome dna methylation through conserved TDRD9/SPN-E function in the germline."
      Shoji M., Tanaka T., Kitamura K., Hosokawa M., Kato Y., Kondoh G., Okawa K., Sasaki H., Chuma S., Nakatsuji N.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    4. "The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline."
      Shoji M., Tanaka T., Hosokawa M., Reuter M., Stark A., Kato Y., Kondoh G., Okawa K., Chujo T., Suzuki T., Hata K., Martin S.L., Noce T., Kuramochi-Miyagawa S., Nakano T., Sasaki H., Pillai R.S., Nakatsuji N., Chuma S.
      Dev. Cell 17:775-787(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH PIWIL4.
    5. "Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
      Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
      Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIWIL1 AND PIWIL4.
    6. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTDRD9_MOUSE
    AccessioniPrimary (citable) accession number: Q14BI7
    Secondary accession number(s): B1Q3J8, Q14AW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 2, 2010
    Last modified: October 1, 2014
    This is version 73 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3