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Q14BI7 (TDRD9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative ATP-dependent RNA helicase TDRD9

EC=3.6.4.13
Alternative name(s):
Tudor domain-containing protein 9
Gene names
Name:Tdrd9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1383 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process. Ref.4

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with piRNA-associated proteins PIWIL1 and PIWIL4. Ref.4 Ref.5

Subcellular location

Cytoplasm. Nucleus. Note: Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies. Ref.4 Ref.6

Tissue specificity

Predominantly expressed in reproductive organs. Detected in mitotic spermatogonia, meiotic spermatocytes (predominantly at the pachytene stage), haploid spermatids in the testis, and in growing oocytes in the ovary (at protein level). Ref.4

Disruption phenotype

Mice are viable but show male sterility with chromosome synapsis failure. In fetal testes, LINE-1 (L1) transposable elements derepression and an aberrant piRNA profile in prospermatogonia, followed by cognate DNA demethylation are observed. Ref.4

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 Tudor domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q14BI7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q14BI7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-401: Missing.
Isoform 3 (identifier: Q14BI7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-772: Missing.
     773-813: PKTTVVLKHI...KSIVFDGAKA → MDIGTKCTSQ...SEESCGVPRA
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13831383Putative ATP-dependent RNA helicase TDRD9
PRO_0000333814

Regions

Domain144 – 310167Helicase ATP-binding
Domain378 – 545168Helicase C-terminal
Domain945 – 100561Tudor
Nucleotide binding157 – 1648ATP By similarity
Motif256 – 2594DEAH box

Natural variations

Alternative sequence1 – 772772Missing in isoform 3.
VSP_033553
Alternative sequence1 – 401401Missing in isoform 2.
VSP_033554
Alternative sequence773 – 81341PKTTV…DGAKA → MDIGTKCTSQVAAGVTAWHL WRRSGPGRLADSEESCGVPR A in isoform 3.
VSP_033555

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 2, 2010. Version 3.
Checksum: 55946F4F639B5777

FASTA1,383155,980
        10         20         30         40         50         60 
MLRKLTVDQI NDWFTIGKTV TNVELLGLPP AFPAEAPREE VQRSEEVPNE DPTAQAQVPV 

        70         80         90        100        110        120 
KATAPARPAS TSGRSLSQRS SEMEYINKYR QLEEQELDIY GQDQPPSGPG LRSPLAKLSN 

       130        140        150        160        170        180 
VACIPETTYK YPDLPINRCK EEVISLIESN SVVIIHGATG SGKSTQLPQY VLDHYTQRSA 

       190        200        210        220        230        240 
FCNIVVTQPR KIGASSIARW ISKERSWTLG GLVGYQVGLE KIATEDTRLI YMTTGVLLQK 

       250        260        270        280        290        300 
IVSAKSLMEF THIFIDEVHE RTEEMDFLLL VVRKLLRTNS RFVKVVLMSA TINCKQFADY 

       310        320        330        340        350        360 
FAVPVQNKMN PAYVFEVEGK PHAIEEYYLN DLGHIYHSGL PYRLEEPVIT KDVYEVAVSL 

       370        380        390        400        410        420 
IQMFDDLDMK ESGNKTWSGA QFVSERSSVL VFLPGLGEIN YMHELLTNMI HKRLQVYPLH 

       430        440        450        460        470        480 
SSVTLEEQNN VFLSPVPGYR KIILSTNIAE SSVTVPDVKY VIDFCLTRTL VCDEDTNYQS 

       490        500        510        520        530        540 
LRLSWASKTS CDQRKGRAGR VSKGYCYRLI PRDFWDSAIP DHVVPEMLRC PLGSTILKVK 

       550        560        570        580        590        600 
LLDMGEPRAL LATALSPPSL SDIERTILLL KEVGALAVSG QREDENPHDG ELTFLGRVLA 

       610        620        630        640        650        660 
QLPVSQQLGK LVVLGHVFGC LDECLIIAAA LSLKNFFTMP FRQHLDGYRN KVHFSGSSRS 

       670        680        690        700        710        720 
DCLALVEAFR AWQACRQRGE LRRPKDELDW GRLNYIQIKR IREVAELYEE LKNRISQFNM 

       730        740        750        760        770        780 
FVGPHHPVLD QEYPYKQRFI LQVVLAGAFY PNYFTFGQPD EEMAVRELAG KDPKTTVVLK 

       790        800        810        820        830        840 
HIPPYGFLYY KQLQSLFRQC GQVKSIVFDG AKAFVEFSRN PTERFKTLPA VNLAVKMSQL 

       850        860        870        880        890        900 
KVSLELSVHA AEEIEGKVQG GSVSKLRNTR VNVDFQKQTV DPMQVSFNTL DRPRTVADLL 

       910        920        930        940        950        960 
LTIDVTEVVE VGHFWGYRID ERNAELLKQL TAEINRLELV PLPIHPHPDL VCLAPFTDYN 

       970        980        990       1000       1010       1020 
KESYFRAQIL YVSGNSAEVF FVDYGNRSHV DLDLLREIPC QFLELPFQAL EFKICKMRPS 

      1030       1040       1050       1060       1070       1080 
AKSLICGEHW SGGAHGRFAA LVGGCPLLVK VFSIVHSVLH VDVYRYSGAQ DAVNVRDVLI 

      1090       1100       1110       1120       1130       1140 
REGYAELAEE SYESKQSYEV LKGFFAKSVD TMPDGSVSSP LKDDEKHLLR ILLESFASNR 

      1150       1160       1170       1180       1190       1200 
LGAPNCKAVL HGPFNPYELK CHSLTRISKF RCVWIEKESI NSVVISDSPA DLHQRMLVAA 

      1210       1220       1230       1240       1250       1260 
SLSVNETGST MLLRETSLMP HIPGLPALLS MLFAPVMELR VDREGKCYTG VLCGLGWNSA 

      1270       1280       1290       1300       1310       1320 
TEAPILPEHD IELAFDVRLN VEDIVEINIL RAAINKLVCD GPNGSKYLGP ERIAQLQENA 

      1330       1340       1350       1360       1370       1380 
RQKLLGLFCR LKPREKITPQ WHEKPYEWNQ VDPRLIMEQA EPEGSPGKST SLYQLHTPVV 


LSP 

« Hide

Isoform 2 [UniParc].

Checksum: 0F9C78208DD4792F
Show »

FASTA982110,899
Isoform 3 [UniParc].

Checksum: 43784340033630A0
Show »

FASTA61168,306

References

« Hide 'large scale' references
[1]"Regulation of retroelement expression and genome dna methylation through conserved TDRD9/SPN-E function in the germline."
Shoji M., Tanaka T., Kitamura K., Hosokawa M., Kato Y., Kondoh G., Okawa K., Sasaki H., Chuma S., Nakatsuji N.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Testis.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
[4]"The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline."
Shoji M., Tanaka T., Hosokawa M., Reuter M., Stark A., Kato Y., Kondoh G., Okawa K., Chujo T., Suzuki T., Hata K., Martin S.L., Noce T., Kuramochi-Miyagawa S., Nakano T., Sasaki H., Pillai R.S., Nakatsuji N., Chuma S.
Dev. Cell 17:775-787(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH PIWIL4.
[5]"Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PIWIL1 AND PIWIL4.
[6]"Cytoplasmic compartmentalization of the fetal piRNA pathway in mice."
Aravin A.A., van der Heijden G.W., Castaneda J., Vagin V.V., Hannon G.J., Bortvin A.
PLoS Genet. 5:E1000764-E1000764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB362563 mRNA. Translation: BAG15992.1.
AC112520 Genomic DNA. No translation available.
AC132623 Genomic DNA. No translation available.
BC115831 mRNA. Translation: AAI15832.1.
BC116656 mRNA. Translation: AAI16657.1.
RefSeqNP_083332.1. NM_029056.1.
XP_006516384.1. XM_006516321.1.
UniGeneMm.60648.

3D structure databases

ProteinModelPortalQ14BI7.
SMRQ14BI7. Positions 119-788, 918-1082.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ14BI7.

Proteomic databases

PaxDbQ14BI7.
PRIDEQ14BI7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000079009; ENSMUSP00000078022; ENSMUSG00000054003. [Q14BI7-1]
GeneID74691.
KEGGmmu:74691.
UCSCuc007pei.1. mouse. [Q14BI7-1]
uc007pej.1. mouse. [Q14BI7-3]

Organism-specific databases

CTD122402.
MGIMGI:1921941. Tdrd9.

Phylogenomic databases

eggNOGCOG1643.
GeneTreeENSGT00740000115350.
HOGENOMHOG000047965.
HOVERGENHBG072266.
InParanoidB1Q3J8.
OMAINDWFTI.
OrthoDBEOG7FV3PF.
PhylomeDBQ14BI7.
TreeFamTF324869.

Gene expression databases

BgeeQ14BI7.
GenevestigatorQ14BI7.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio341402.
PROQ14BI7.
SOURCESearch...

Entry information

Entry nameTDRD9_MOUSE
AccessionPrimary (citable) accession number: Q14BI7
Secondary accession number(s): B1Q3J8, Q14AW6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 2, 2010
Last modified: April 16, 2014
This is version 68 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot