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Q14BI7

- TDRD9_MOUSE

UniProt

Q14BI7 - TDRD9_MOUSE

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Protein
Putative ATP-dependent RNA helicase TDRD9
Gene
Tdrd9
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with PIWIL4 and the piP-bodies suggests a participation in the secondary piRNAs metabolic process.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi157 – 1648ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. nucleic acid binding Source: InterPro
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. DNA methylation involved in gamete generation Source: UniProtKB
  2. cell differentiation Source: UniProtKB-KW
  3. fertilization Source: UniProtKB
  4. gene silencing by RNA Source: UniProtKB-KW
  5. male meiosis Source: UniProtKB
  6. multicellular organismal development Source: UniProtKB-KW
  7. piRNA metabolic process Source: UniProtKB
  8. spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase TDRD9 (EC:3.6.4.13)
Alternative name(s):
Tudor domain-containing protein 9
Gene namesi
Name:Tdrd9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1921941. Tdrd9.

Subcellular locationi

Cytoplasm. Nucleus
Note: Component of the nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. PIWIL2 is required for its localization to piP-bodies.2 Publications

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. piP-body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable but show male sterility with chromosome synapsis failure. In fetal testes, LINE-1 (L1) transposable elements derepression and an aberrant piRNA profile in prospermatogonia, followed by cognate DNA demethylation are observed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13831383Putative ATP-dependent RNA helicase TDRD9
PRO_0000333814Add
BLAST

Proteomic databases

PaxDbiQ14BI7.
PRIDEiQ14BI7.

PTM databases

PhosphoSiteiQ14BI7.

Expressioni

Tissue specificityi

Predominantly expressed in reproductive organs. Detected in mitotic spermatogonia, meiotic spermatocytes (predominantly at the pachytene stage), haploid spermatids in the testis, and in growing oocytes in the ovary (at protein level).1 Publication

Gene expression databases

BgeeiQ14BI7.
GenevestigatoriQ14BI7.

Interactioni

Subunit structurei

Interacts with piRNA-associated proteins PIWIL1 and PIWIL4.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ14BI7.
SMRiQ14BI7. Positions 126-720, 918-1082.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini144 – 310167Helicase ATP-binding
Add
BLAST
Domaini378 – 545168Helicase C-terminal
Add
BLAST
Domaini945 – 100561Tudor
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi256 – 2594DEAH box

Sequence similaritiesi

Contains 1 Tudor domain.

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00740000115350.
HOGENOMiHOG000047965.
HOVERGENiHBG072266.
InParanoidiB1Q3J8.
OMAiINDWFTI.
OrthoDBiEOG7FV3PF.
PhylomeDBiQ14BI7.
TreeFamiTF324869.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q14BI7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLRKLTVDQI NDWFTIGKTV TNVELLGLPP AFPAEAPREE VQRSEEVPNE     50
DPTAQAQVPV KATAPARPAS TSGRSLSQRS SEMEYINKYR QLEEQELDIY 100
GQDQPPSGPG LRSPLAKLSN VACIPETTYK YPDLPINRCK EEVISLIESN 150
SVVIIHGATG SGKSTQLPQY VLDHYTQRSA FCNIVVTQPR KIGASSIARW 200
ISKERSWTLG GLVGYQVGLE KIATEDTRLI YMTTGVLLQK IVSAKSLMEF 250
THIFIDEVHE RTEEMDFLLL VVRKLLRTNS RFVKVVLMSA TINCKQFADY 300
FAVPVQNKMN PAYVFEVEGK PHAIEEYYLN DLGHIYHSGL PYRLEEPVIT 350
KDVYEVAVSL IQMFDDLDMK ESGNKTWSGA QFVSERSSVL VFLPGLGEIN 400
YMHELLTNMI HKRLQVYPLH SSVTLEEQNN VFLSPVPGYR KIILSTNIAE 450
SSVTVPDVKY VIDFCLTRTL VCDEDTNYQS LRLSWASKTS CDQRKGRAGR 500
VSKGYCYRLI PRDFWDSAIP DHVVPEMLRC PLGSTILKVK LLDMGEPRAL 550
LATALSPPSL SDIERTILLL KEVGALAVSG QREDENPHDG ELTFLGRVLA 600
QLPVSQQLGK LVVLGHVFGC LDECLIIAAA LSLKNFFTMP FRQHLDGYRN 650
KVHFSGSSRS DCLALVEAFR AWQACRQRGE LRRPKDELDW GRLNYIQIKR 700
IREVAELYEE LKNRISQFNM FVGPHHPVLD QEYPYKQRFI LQVVLAGAFY 750
PNYFTFGQPD EEMAVRELAG KDPKTTVVLK HIPPYGFLYY KQLQSLFRQC 800
GQVKSIVFDG AKAFVEFSRN PTERFKTLPA VNLAVKMSQL KVSLELSVHA 850
AEEIEGKVQG GSVSKLRNTR VNVDFQKQTV DPMQVSFNTL DRPRTVADLL 900
LTIDVTEVVE VGHFWGYRID ERNAELLKQL TAEINRLELV PLPIHPHPDL 950
VCLAPFTDYN KESYFRAQIL YVSGNSAEVF FVDYGNRSHV DLDLLREIPC 1000
QFLELPFQAL EFKICKMRPS AKSLICGEHW SGGAHGRFAA LVGGCPLLVK 1050
VFSIVHSVLH VDVYRYSGAQ DAVNVRDVLI REGYAELAEE SYESKQSYEV 1100
LKGFFAKSVD TMPDGSVSSP LKDDEKHLLR ILLESFASNR LGAPNCKAVL 1150
HGPFNPYELK CHSLTRISKF RCVWIEKESI NSVVISDSPA DLHQRMLVAA 1200
SLSVNETGST MLLRETSLMP HIPGLPALLS MLFAPVMELR VDREGKCYTG 1250
VLCGLGWNSA TEAPILPEHD IELAFDVRLN VEDIVEINIL RAAINKLVCD 1300
GPNGSKYLGP ERIAQLQENA RQKLLGLFCR LKPREKITPQ WHEKPYEWNQ 1350
VDPRLIMEQA EPEGSPGKST SLYQLHTPVV LSP 1383
Length:1,383
Mass (Da):155,980
Last modified:March 2, 2010 - v3
Checksum:i55946F4F639B5777
GO
Isoform 2 (identifier: Q14BI7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-401: Missing.

Show »
Length:982
Mass (Da):110,899
Checksum:i0F9C78208DD4792F
GO
Isoform 3 (identifier: Q14BI7-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-772: Missing.
     773-813: PKTTVVLKHI...KSIVFDGAKA → MDIGTKCTSQ...SEESCGVPRA

Note: No experimental confirmation available.

Show »
Length:611
Mass (Da):68,306
Checksum:i43784340033630A0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 772772Missing in isoform 3.
VSP_033553Add
BLAST
Alternative sequencei1 – 401401Missing in isoform 2.
VSP_033554Add
BLAST
Alternative sequencei773 – 81341PKTTV…DGAKA → MDIGTKCTSQVAAGVTAWHL WRRSGPGRLADSEESCGVPR A in isoform 3.
VSP_033555Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB362563 mRNA. Translation: BAG15992.1.
AC112520 Genomic DNA. No translation available.
AC132623 Genomic DNA. No translation available.
BC115831 mRNA. Translation: AAI15832.1.
BC116656 mRNA. Translation: AAI16657.1.
CCDSiCCDS49184.1. [Q14BI7-1]
RefSeqiNP_083332.1. NM_029056.1. [Q14BI7-1]
XP_006516384.1. XM_006516321.1. [Q14BI7-2]
UniGeneiMm.60648.

Genome annotation databases

EnsembliENSMUST00000079009; ENSMUSP00000078022; ENSMUSG00000054003. [Q14BI7-1]
GeneIDi74691.
KEGGimmu:74691.
UCSCiuc007pei.1. mouse. [Q14BI7-1]
uc007pej.1. mouse. [Q14BI7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB362563 mRNA. Translation: BAG15992.1 .
AC112520 Genomic DNA. No translation available.
AC132623 Genomic DNA. No translation available.
BC115831 mRNA. Translation: AAI15832.1 .
BC116656 mRNA. Translation: AAI16657.1 .
CCDSi CCDS49184.1. [Q14BI7-1 ]
RefSeqi NP_083332.1. NM_029056.1. [Q14BI7-1 ]
XP_006516384.1. XM_006516321.1. [Q14BI7-2 ]
UniGenei Mm.60648.

3D structure databases

ProteinModelPortali Q14BI7.
SMRi Q14BI7. Positions 126-720, 918-1082.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q14BI7.

Proteomic databases

PaxDbi Q14BI7.
PRIDEi Q14BI7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000079009 ; ENSMUSP00000078022 ; ENSMUSG00000054003 . [Q14BI7-1 ]
GeneIDi 74691.
KEGGi mmu:74691.
UCSCi uc007pei.1. mouse. [Q14BI7-1 ]
uc007pej.1. mouse. [Q14BI7-3 ]

Organism-specific databases

CTDi 122402.
MGIi MGI:1921941. Tdrd9.

Phylogenomic databases

eggNOGi COG1643.
GeneTreei ENSGT00740000115350.
HOGENOMi HOG000047965.
HOVERGENi HBG072266.
InParanoidi B1Q3J8.
OMAi INDWFTI.
OrthoDBi EOG7FV3PF.
PhylomeDBi Q14BI7.
TreeFami TF324869.

Miscellaneous databases

NextBioi 341402.
PROi Q14BI7.
SOURCEi Search...

Gene expression databases

Bgeei Q14BI7.
Genevestigatori Q14BI7.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002999. Tudor.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00567. TUDOR. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00333. TUDOR. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50304. TUDOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of retroelement expression and genome dna methylation through conserved TDRD9/SPN-E function in the germline."
    Shoji M., Tanaka T., Kitamura K., Hosokawa M., Kato Y., Kondoh G., Okawa K., Sasaki H., Chuma S., Nakatsuji N.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
  4. "The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline."
    Shoji M., Tanaka T., Hosokawa M., Reuter M., Stark A., Kato Y., Kondoh G., Okawa K., Chujo T., Suzuki T., Hata K., Martin S.L., Noce T., Kuramochi-Miyagawa S., Nakano T., Sasaki H., Pillai R.S., Nakatsuji N., Chuma S.
    Dev. Cell 17:775-787(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH PIWIL4.
  5. "Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
    Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
    Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIWIL1 AND PIWIL4.
  6. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTDRD9_MOUSE
AccessioniPrimary (citable) accession number: Q14BI7
Secondary accession number(s): B1Q3J8, Q14AW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 2, 2010
Last modified: September 3, 2014
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi