Q14B80 (KCNC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily C member 2

Alternative name(s):
Voltage-gated potassium channel Kv3.2

Gene names

Name:

Kcnc2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Channel properties are modulated by subunit assembly. Ref.3
Subunit structure	The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits that can associate with modulating accessory subunits. Interacts with KCNC1, KCNE1, KCNE2 and KCNE3 By similarity. Ref.2
Subcellular location	Membrane; Multi-pass membrane protein Ref.2.
Tissue specificity	Detected in hippocampus. Ref.2
Developmental stage	Not detectable in hippocampus from newborns. Detected at low levels in developing hippocampus from day 7 to 12. Levels are much increased after 14 days. Expressed at high and stable levels in hippocampus after 21 days (at protein level). Ref.2
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Sequence similarities	Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.2/KCNC2 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein homooligomerization Inferred from electronic annotation. Source: InterPro regulation of action potential Inferred from mutant phenotype Ref.3. Source: MGI
Cellular_component	voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	Potassium voltage-gated channel subfamily C member 2		PRO_0000310417
Regions
	Topological domain	1 – 233	233	Cytoplasmic Potential
	Transmembrane	234 – 254	21	Helical; Name=Segment S1; Potential
	Transmembrane	287 – 307	21	Helical; Name=Segment S2; Potential
	Topological domain	308 – 317	10	Cytoplasmic Potential
	Transmembrane	318 – 338	21	Helical; Name=Segment S3; Potential
	Transmembrane	350 – 372	23	Helical; Voltage-sensor; Name=Segment S4; Potential
	Topological domain	373 – 385	13	Cytoplasmic Potential
	Transmembrane	386 – 406	21	Helical; Name=Segment S5; Potential
	Transmembrane	457 – 477	21	Helical; Name=Segment S6; Potential
	Topological domain	478 – 642	165	Cytoplasmic Potential
	Motif	441 – 446	6	Selectivity filter By similarity
	Compositional bias	56 – 103	48	Gly/Pro-rich (insert)
Amino acid modifications
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q14B80 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 2B9B6D29A40A80E3	FASTA	642	70,503
	10 20 30 40 50 60 MGKIESNERV ILNVGGTRHE TYRSTLKTLP GTRLALLASS EPQGDCLTAA GDKLQPLPPP 70 80 90 100 110 120 LSPPPRPPPL SPVPSGCFEG GAGNCSSHGG NGGNGGSDHP GGGREFFFDR HPGVFAYVLN 130 140 150 160 170 180 YYRTGKLHCP ADVCGPLFEE ELAFWGIDET DVEPCCWMTY RQHRDAEEAL DIFETPDLIG 190 200 210 220 230 240 GDPGDDEDLA AKRLGIEDAA GLGGPDGKSG RWRKLQPRMW ALFEDPYSSR AARFIAFASL 250 260 270 280 290 300 FFILVSITTF CLETHEAFNI VKNKTEPVIN GTSPVLQYEI ETDPALTYVE GVCVVWFTFE 310 320 330 340 350 360 FLVRIVFSPN KLEFIKNLLN IIDFVAILPF YLEVGLSGLS SKAAKDVLGF LRVVRFVRIL 370 380 390 400 410 420 RIFKLTRHFV GLRVLGHTLR ASTNEFLLLI IFLALGVLIF ATMIYYAERV GAQPNDPSAS 430 440 450 460 470 480 EHTQFKNIPI GFWWAVVTMT TLGYGDMYPQ TWSGMLVGAL CALAGVLTIA MPVPVIVNNF 490 500 510 520 530 540 GMYYSLAMAK QKLPRKRKKH IPPAPLASSP TFCKTELNMA CNSTQSDTCL GKENRLLEHN 550 560 570 580 590 600 RSVLSGDDST GSEPPLSPPE RLPIRRSSTR DKNRRGETCF LLTTGDYTCA SDGGIRKGYE 610 620 630 640 KSRSLNNIAG LAGNALRLSP VTSPYNSPCP LRRSRSPIPS IL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Developmental expression of potassium-channel subunit Kv3.2 within subpopulations of mouse hippocampal inhibitory interneurons." Tansey E.P., Chow A., Rudy B., McBain C.J. Hippocampus 12:137-148(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INTERACTION WITH KCNC1, TISSUE SPECIFICITY.
[3]	"Differential regulation of action potential firing in adult murine thalamocortical neurons by Kv3.2, Kv1, and SK potassium and N-type calcium channels." Kasten M.R., Rudy B., Anderson M.P. J. Physiol. (Lond.) 584:565-582(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC116289 mRNA. Translation: AAI16290.1. BC116290 mRNA. Translation: AAI16291.1.
IPI	IPI00457867.
UniGene	Mm.336242.
3D structure databases
ProteinModelPortal	Q14B80.
SMR	Q14B80. Positions 105-484.
ModBase	Search...
PTM databases
PhosphoSite	Q14B80.
Proteomic databases
PaxDb	Q14B80.
PRIDE	Q14B80.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	uc007hao.1. mouse.
Organism-specific databases
MGI	MGI:96668. Kcnc2.
Phylogenomic databases
eggNOG	COG1226.
HOGENOM	HOG000231012.
HOVERGEN	HBG105862.
InParanoid	Q14B80.
OrthoDB	EOG4JQ3X8.
Gene expression databases
Genevestigator	Q14B80.
Family and domain databases
Gene3D	3.30.710.10. 2 hits.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003974. K_chnl_volt-dep_Kv3. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 2 hits. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01491. KVCHANNEL. PR01498. SHAWCHANNEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	KCNC2. mouse.
SOURCE	Search...

Entry information

Entry name

KCNC2_MOUSE

Accession

Primary (citable) accession number: Q14B80

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program