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Protein

E3 ubiquitin-protein ligase RNF133

Gene

Rnf133

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has E3 ubiquitin-protein ligase activity.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri256 – 297RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

  • protein autoubiquitination Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF133 (EC:2.3.2.27)
Alternative name(s):
Goliath-related E3 ubiquitin-protein ligase 2
RING finger protein 133
RING-type E3 ubiquitin transferase RNF133Curated
Gene namesi
Name:Rnf133
Synonyms:Greul2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2677436. Rnf133.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003807511 – 382E3 ubiquitin-protein ligase RNF133Add BLAST382

Post-translational modificationi

Auto-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ14B02.

PTM databases

iPTMnetiQ14B02.
PhosphoSitePlusiQ14B02.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Developmental stagei

Expression begins in the testis at day 21 and increases dramatically from day 28 and thereafter.1 Publication

Gene expression databases

BgeeiENSMUSG00000051956.
GenevisibleiQ14B02. MM.

Structurei

3D structure databases

ProteinModelPortaliQ14B02.
SMRiQ14B02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 167PAAdd BLAST103

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri256 – 297RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000119057.
HOGENOMiHOG000231432.
InParanoidiQ14B02.
KOiK15702.
OMAiMNISFHV.
OrthoDBiEOG091G08OR.
TreeFamiTF317486.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR003137. PA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14B02-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPLQTSTWQ NQAPSFWLLR FSFIWLVSQK CCTASAVWTA YMNISFHVGN
60 70 80 90 100
RMLSELGETG VFGRSSILKR VAGVVVPPEG KIQNACDPNT TFILPRNKEP
110 120 130 140 150
WIALIERGGC AFTQKIKVAS EHGARGVIIY NFPGTGNQVF PMSHQAFEDI
160 170 180 190 200
VVVMIGNIKG MEILHLIRKG VHVTVMVEVG RKHVIWLNHY FVSFMIVTTA
210 220 230 240 250
TLAYFTFYHI RRLWVARIEN RRWKRLTREL KKAFGQLQVR VLKEGDEEVN
260 270 280 290 300
PNADSCVICF EAYKPNEIVR ILTCKHFFHK NCIDPWILAH GTCPMCKCDI
310 320 330 340 350
LKALGIQMDI EDGTDSLQVL MSNELPGTLS PVEEETNYEL PPARTSSKVT
360 370 380
HVQEHPTSSA NAGSQPPEAE ETSHPSHGQQ VL
Length:382
Mass (Da):43,102
Last modified:August 22, 2006 - v1
Checksum:i3EC4A20E7B5CF4D0
GO
Isoform 2 (identifier: Q14B02-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-202: Missing.

Show »
Length:339
Mass (Da):38,143
Checksum:i5248DFA7C8EE1ABF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182K → E in AAN75222 (PubMed:12435366).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037829160 – 202Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028082 mRNA. Translation: BAC25740.1.
CH466533 Genomic DNA. Translation: EDL13834.1.
BC116423 mRNA. Translation: AAI16424.1.
BC116424 mRNA. Translation: AAI16425.1.
AY155441 mRNA. Translation: AAN75222.1.
CCDSiCCDS19938.1. [Q14B02-2]
RefSeqiNP_937894.1. NM_198251.2. [Q14B02-2]
UniGeneiMm.436547.

Genome annotation databases

EnsembliENSMUST00000063548; ENSMUSP00000066906; ENSMUSG00000051956. [Q14B02-2]
ENSMUST00000115354; ENSMUSP00000111011; ENSMUSG00000051956. [Q14B02-1]
GeneIDi386611.
KEGGimmu:386611.
UCSCiuc009bbk.1. mouse. [Q14B02-2]
uc029vtq.1. mouse. [Q14B02-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRN133_MOUSE
AccessioniPrimary (citable) accession number: Q14B02
Secondary accession number(s): Q8C1F1, Q8CGR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: August 30, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways