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Protein

DnaJ homolog subfamily B member 14

Gene

Dnajb14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a co-chaperone with HSPA8 and promotes the degradation of misfolded transmembrane proteins in the ER-associated degradation (ERAD) pathway.By similarity

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 14
Gene namesi
Name:Dnajb14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917854. Dnajb14.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 244244CytoplasmicSequence analysisAdd
BLAST
Transmembranei245 – 26521HelicalSequence analysisAdd
BLAST
Topological domaini266 – 379114LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379DnaJ homolog subfamily B member 14PRO_0000281479Add
BLAST

Proteomic databases

MaxQBiQ149L6.
PaxDbiQ149L6.
PeptideAtlasiQ149L6.
PRIDEiQ149L6.

PTM databases

iPTMnetiQ149L6.
PhosphoSiteiQ149L6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000074212.
CleanExiMM_DNAJB14.
ExpressionAtlasiQ149L6. baseline and differential.
GenevisibleiQ149L6. MM.

Interactioni

Subunit structurei

Interacts (via J domain) with HSPA8/Hsc70.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087641.

Structurei

3D structure databases

ProteinModelPortaliQ149L6.
SMRiQ149L6. Positions 107-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 17265JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00840000129767.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ149L6.
KOiK09520.
OMAiKKAELMT.
OrthoDBiEOG091G0CE9.
PhylomeDBiQ149L6.
TreeFamiTF105145.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q149L6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGNRDEAEK CVQIAREALS AGNRDKAQRF LQKAEKLYPL PAARALLEII
60 70 80 90 100
MKNGSTAGSS THCRKPPGSS DQSKPSCGKD GTSGAGEGGK VYTKDQVEGV
110 120 130 140 150
LSINKCKNYY EVLGVTKDAG DEDLKKAYRK LALKFHPDKN HAPGATDAFK
160 170 180 190 200
KIGNAYAVLS NPEKRKQYDL TGSEEQACNH QNNGRFNFHR GCEADITPED
210 220 230 240 250
LFNIFFGGGF PSGSVHSFSN GRAAYSHQHQ HRHSGHEREE ERADGGFSVF
260 270 280 290 300
IQLMPIIVLI LVSLLSQLMV SNPPYSLYPR SGSGQTIKMQ TENLGVVYYV
310 320 330 340 350
SKDFKSEYKG TLLQKVEKSV EEDYVTNIRN NCWKERQQKT DMQYAAKVYR
360 370
DEQLRRKADA LSMENCKELE RLTSLYKGG
Length:379
Mass (Da):42,326
Last modified:August 22, 2006 - v1
Checksum:i83C79C1C51A18D82
GO
Isoform 2 (identifier: Q149L6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):36,717
Checksum:i09A8B5C65EF1631F
GO
Isoform 3 (identifier: Q149L6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: KIGNAYAV → SKVTYDTS
     159-379: Missing.

Note: No experimental confirmation available.
Show »
Length:158
Mass (Da):17,053
Checksum:iC2D64C45BBF4FED8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5050Missing in isoform 2. 1 PublicationVSP_024008Add
BLAST
Alternative sequencei151 – 1588KIGNAYAV → SKVTYDTS in isoform 3. 1 PublicationVSP_024009
Alternative sequencei159 – 379221Missing in isoform 3. 1 PublicationVSP_024010Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139489 mRNA. Translation: BAE24035.1.
AK160961 mRNA. Translation: BAE36117.1.
BC117717 mRNA. Translation: AAI17718.1.
BC117718 mRNA. Translation: AAI17719.1.
CCDSiCCDS51077.1. [Q149L6-1]
RefSeqiNP_001028327.1. NM_001033155.1. [Q149L6-1]
XP_006502118.1. XM_006502055.2. [Q149L6-2]
UniGeneiMm.256488.
Mm.392179.
Mm.392730.

Genome annotation databases

EnsembliENSMUST00000090178; ENSMUSP00000087641; ENSMUSG00000074212. [Q149L6-1]
GeneIDi70604.
KEGGimmu:70604.
UCSCiuc008rmq.2. mouse. [Q149L6-3]
uc008rmr.2. mouse. [Q149L6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139489 mRNA. Translation: BAE24035.1.
AK160961 mRNA. Translation: BAE36117.1.
BC117717 mRNA. Translation: AAI17718.1.
BC117718 mRNA. Translation: AAI17719.1.
CCDSiCCDS51077.1. [Q149L6-1]
RefSeqiNP_001028327.1. NM_001033155.1. [Q149L6-1]
XP_006502118.1. XM_006502055.2. [Q149L6-2]
UniGeneiMm.256488.
Mm.392179.
Mm.392730.

3D structure databases

ProteinModelPortaliQ149L6.
SMRiQ149L6. Positions 107-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087641.

PTM databases

iPTMnetiQ149L6.
PhosphoSiteiQ149L6.

Proteomic databases

MaxQBiQ149L6.
PaxDbiQ149L6.
PeptideAtlasiQ149L6.
PRIDEiQ149L6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090178; ENSMUSP00000087641; ENSMUSG00000074212. [Q149L6-1]
GeneIDi70604.
KEGGimmu:70604.
UCSCiuc008rmq.2. mouse. [Q149L6-3]
uc008rmr.2. mouse. [Q149L6-1]

Organism-specific databases

CTDi79982.
MGIiMGI:1917854. Dnajb14.

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00840000129767.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ149L6.
KOiK09520.
OMAiKKAELMT.
OrthoDBiEOG091G0CE9.
PhylomeDBiQ149L6.
TreeFamiTF105145.

Miscellaneous databases

PROiQ149L6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074212.
CleanExiMM_DNAJB14.
ExpressionAtlasiQ149L6. baseline and differential.
GenevisibleiQ149L6. MM.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDJB14_MOUSE
AccessioniPrimary (citable) accession number: Q149L6
Secondary accession number(s): Q3TU54, Q3UTE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: August 22, 2006
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.