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Protein

DnaJ homolog subfamily B member 14

Gene

Dnajb14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 14Imported
Gene namesi
Name:Dnajb14Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917854. Dnajb14.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
  • Nucleus membrane By similarity; Single-pass membrane protein Sequence analysis

  • Note: Localizes to the endoplasmic reticulum membrane. When overexpressed, forms membranous structures in the nucleus.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 244CytoplasmicSequence analysisAdd BLAST244
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 379LumenalSequence analysisAdd BLAST114

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002814791 – 379DnaJ homolog subfamily B member 14Add BLAST379

Proteomic databases

MaxQBiQ149L6.
PaxDbiQ149L6.
PeptideAtlasiQ149L6.
PRIDEiQ149L6.

PTM databases

iPTMnetiQ149L6.
PhosphoSitePlusiQ149L6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000074212.
CleanExiMM_DNAJB14.
ExpressionAtlasiQ149L6. baseline and differential.
GenevisibleiQ149L6. MM.

Interactioni

Subunit structurei

Interacts (via J domain) with HSPA8/Hsc70. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087641.

Structurei

3D structure databases

ProteinModelPortaliQ149L6.
SMRiQ149L6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 172JPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00840000129767.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ149L6.
KOiK09520.
OMAiMENCKEL.
OrthoDBiEOG091G0CE9.
PhylomeDBiQ149L6.
TreeFamiTF105145.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q149L6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGNRDEAEK CVQIAREALS AGNRDKAQRF LQKAEKLYPL PAARALLEII
60 70 80 90 100
MKNGSTAGSS THCRKPPGSS DQSKPSCGKD GTSGAGEGGK VYTKDQVEGV
110 120 130 140 150
LSINKCKNYY EVLGVTKDAG DEDLKKAYRK LALKFHPDKN HAPGATDAFK
160 170 180 190 200
KIGNAYAVLS NPEKRKQYDL TGSEEQACNH QNNGRFNFHR GCEADITPED
210 220 230 240 250
LFNIFFGGGF PSGSVHSFSN GRAAYSHQHQ HRHSGHEREE ERADGGFSVF
260 270 280 290 300
IQLMPIIVLI LVSLLSQLMV SNPPYSLYPR SGSGQTIKMQ TENLGVVYYV
310 320 330 340 350
SKDFKSEYKG TLLQKVEKSV EEDYVTNIRN NCWKERQQKT DMQYAAKVYR
360 370
DEQLRRKADA LSMENCKELE RLTSLYKGG
Length:379
Mass (Da):42,326
Last modified:August 22, 2006 - v1
Checksum:i83C79C1C51A18D82
GO
Isoform 2 (identifier: Q149L6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Note: No experimental confirmation available.
Show »
Length:329
Mass (Da):36,717
Checksum:i09A8B5C65EF1631F
GO
Isoform 3 (identifier: Q149L6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-158: KIGNAYAV → SKVTYDTS
     159-379: Missing.

Note: No experimental confirmation available.
Show »
Length:158
Mass (Da):17,053
Checksum:iC2D64C45BBF4FED8
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0240081 – 50Missing in isoform 2. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_024009151 – 158KIGNAYAV → SKVTYDTS in isoform 3. 1 Publication8
Alternative sequenceiVSP_024010159 – 379Missing in isoform 3. 1 PublicationAdd BLAST221

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139489 mRNA. Translation: BAE24035.1.
AK160961 mRNA. Translation: BAE36117.1.
BC117717 mRNA. Translation: AAI17718.1.
BC117718 mRNA. Translation: AAI17719.1.
CCDSiCCDS51077.1. [Q149L6-1]
RefSeqiNP_001028327.1. NM_001033155.1. [Q149L6-1]
XP_006502118.1. XM_006502055.2. [Q149L6-2]
UniGeneiMm.256488.
Mm.392179.
Mm.392730.

Genome annotation databases

EnsembliENSMUST00000090178; ENSMUSP00000087641; ENSMUSG00000074212. [Q149L6-1]
GeneIDi70604.
KEGGimmu:70604.
UCSCiuc008rmq.2. mouse. [Q149L6-3]
uc008rmr.2. mouse. [Q149L6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDJB14_MOUSE
AccessioniPrimary (citable) accession number: Q149L6
Secondary accession number(s): Q3TU54, Q3UTE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: August 22, 2006
Last modified: June 7, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families