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Protein

Eukaryotic peptide chain release factor GTP-binding subunit ERF3B

Gene

Gspt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei220Interacts with GTP/GDPBy similarity1
Sitei354Interacts with GTP/GDPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi214 – 221GTPBy similarity8
Nucleotide bindingi291 – 295GTPBy similarity5
Nucleotide bindingi353 – 356GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Nonsense-mediated mRNA decay, Protein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-72764 Eukaryotic Translation Termination
R-MMU-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3B
Short name:
Eukaryotic peptide chain release factor subunit 3b
Short name:
eRF3b
Alternative name(s):
G1 to S phase transition protein 2 homolog
Gene namesi
Name:Gspt2
Synonyms:Erf3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1316727 Gspt2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003272571 – 632Eukaryotic peptide chain release factor GTP-binding subunit ERF3BAdd BLAST632

Proteomic databases

MaxQBiQ149F3
PaxDbiQ149F3
PeptideAtlasiQ149F3
PRIDEiQ149F3

PTM databases

iPTMnetiQ149F3
PhosphoSitePlusiQ149F3
SwissPalmiQ149F3

Expressioni

Tissue specificityi

Highly expressed in brain. Moderately expressed in spleen and lung. Weakly expressed in heart, liver and kidney. Expression during the cell-cycle progression is constant.1 Publication

Developmental stagei

Barely detectable at E15, increased after birth to reach a maximum at P15.1 Publication

Gene expression databases

BgeeiENSMUSG00000071723
ExpressionAtlasiQ149F3 baseline and differential
GenevisibleiQ149F3 MM

Interactioni

Subunit structurei

Interacts with ETF1. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with UPF1 and PABPC1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ149F3, 2 interactors
MINTiQ149F3
STRINGi10090.ENSMUSP00000109523

Structurei

3D structure databases

ProteinModelPortaliQ149F3
SMRiQ149F3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 429tr-type GPROSITE-ProRule annotationAdd BLAST225

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221G1PROSITE-ProRule annotation8
Regioni270 – 274G2PROSITE-ProRule annotation5
Regioni291 – 294G3PROSITE-ProRule annotation4
Regioni353 – 356G4PROSITE-ProRule annotation4
Regioni395 – 397G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0459 Eukaryota
COG5256 LUCA
GeneTreeiENSGT00620000087924
HOGENOMiHOG000229291
HOVERGENiHBG000179
InParanoidiQ149F3
KOiK03267
OMAiWEHSKEV
OrthoDBiEOG091G0637
TreeFamiTF300566

Family and domain databases

InterProiView protein in InterPro
IPR009818 Ataxin-2_C
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PF07145 PAM2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q149F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLGSSSDSA PDCWDQVDME APGSAPSGDG IAPAAMAAAE AAEAEAQRKH
60 70 80 90 100
LSLAFSSQLN IHAKPFVPSV SAAEFVPSFL PGSAQPPAPT ASSCDETCIG
110 120 130 140 150
GAGEPEGKRM EWGAPVEPSK DGPLVSWEGS SSVVTMELSE PVVENGEVEM
160 170 180 190 200
ALEESWELKE VSEAKPEASL GDAGPPEESV KEVMEEKEEV RKSKSVSIPS
210 220 230 240 250
GAPKKEHVNV VFIGHVDAGK STIGGQIMFL TGMVDRRTLE KYEREAKEKN
260 270 280 290 300
RETWYLSWAL DTNQEERDKG KTVEVGRAYF ETEKKHFTIL DAPGHKSFVP
310 320 330 340 350
NMIGGASQAD LAVLVISARK GEFETGFEKG GQTREHAMLA KTAGVKYLIV
360 370 380 390 400
LINKMDDPTV DWSSERYEEC KEKLVPFLKK VGFSPKKDIH FMPCSGLTGA
410 420 430 440 450
NIKEQSDFCP WYTGLPFIPY LDSLPNFNRS IDGPIRLPIV DKYKDMGTVV
460 470 480 490 500
LGKLESGSIF KGQQLVMMPN KHSVEVLGIV SDDAETDFVA PGENLKIRLK
510 520 530 540 550
GIEEEEILPG FILCEPSNLC HSGRTFDVQI VIIEHKSIIC PGYNAVLHIH
560 570 580 590 600
TCIEEVEITA LISLVDKKSG EKSKTRPRFV KQDQVCIARL RTAGTICLET
610 620 630
FKDFPQMGRF TLRDEGKTIA IGKVLKLVPE KD
Length:632
Mass (Da):69,147
Last modified:August 22, 2006 - v1
Checksum:i9783F5C867D8EB13
GO

Sequence cautioni

The sequence BAA32527 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB31621 differs from that shown. Reason: Frameshift at position 591.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21A → G in BAA32527 (PubMed:9712840).Curated1
Sequence conflicti84A → P in BAA32527 (PubMed:9712840).Curated1
Sequence conflicti197S → A in BAA32527 (PubMed:9712840).Curated1
Sequence conflicti262T → K in BAB31621 (PubMed:16141072).Curated1
Sequence conflicti510G → A in BAB31621 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019241 mRNA Translation: BAB31621.1 Frameshift.
AL645466 Genomic DNA No translation available.
BC117825 mRNA Translation: AAI17826.1
BC117826 mRNA Translation: AAI17827.1
AB003503 mRNA Translation: BAA32527.1 Different initiation.
CCDSiCCDS41064.1
RefSeqiNP_032205.2, NM_008179.2
UniGeneiMm.20826

Genome annotation databases

EnsembliENSMUST00000096368; ENSMUSP00000109523; ENSMUSG00000071723
GeneIDi14853
KEGGimmu:14853
UCSCiuc009tto.1 mouse

Similar proteinsi

Entry informationi

Entry nameiERF3B_MOUSE
AccessioniPrimary (citable) accession number: Q149F3
Secondary accession number(s): O88180, Q9CY91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: August 22, 2006
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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