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Protein

TRAF3-interacting protein 1

Gene

Traf3ip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an inhibitory role on IL13 signaling by binding to IL13RA1. Involved in suppression of IL13-induced STAT6 phosphorylation, transcriptional activity and DNA-binding. Recruits TRAF3 and DISC1 to the microtubules (By similarity). Involved in epithelial morphogenesis and in the regulation of microtubule cytoskeleton organization. Is a negative regulator of microtubule stability, acting through the control of MAP4 levels (PubMed:26487268). Involved in ciliogenesis (PubMed:21945076).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • cilium assembly Source: MGI
  • embryonic camera-type eye development Source: MGI
  • embryonic digit morphogenesis Source: MGI
  • embryonic heart tube development Source: MGI
  • intraciliary transport Source: MGI
  • kidney development Source: UniProtKB
  • morphogenesis of a polarized epithelium Source: UniProtKB
  • negative regulation of defense response to virus Source: CACAO
  • negative regulation of interferon-beta production Source: MGI
  • negative regulation of protein complex assembly Source: MGI
  • negative regulation of protein phosphorylation Source: MGI
  • negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of type I interferon production Source: MGI
  • negative regulation of tyrosine phosphorylation of Stat6 protein Source: MGI
  • neural tube patterning Source: MGI
  • post-anal tail morphogenesis Source: MGI
  • regulation of DNA binding Source: MGI
  • regulation of microtubule cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
TRAF3-interacting protein 1
Alternative name(s):
Intraflagellar transport protein 54 homolog
Microtubule-interacting protein associated with TRAF3
Short name:
MIP-T3
Gene namesi
Name:Traf3ip1
Synonyms:Ift54, Mipt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1921269. Traf3ip1.

Subcellular locationi

GO - Cellular componenti

  • axoneme Source: MGI
  • centrosome Source: MGI
  • ciliary basal body Source: MGI
  • ciliary base Source: UniProtKB
  • ciliary tip Source: UniProtKB
  • ciliary transition zone Source: UniProtKB
  • cilium Source: MGI
  • cytosol Source: MGI
  • intraciliary transport particle B Source: UniProtKB
  • microtubule cytoskeleton Source: MGI
  • primary cilium Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Homozygous mutant mice are not viable and have neural development defects, abnormal neural tube patterning, polydactyly, cardiac edema, and variable microphthalmia. Mutant cells have a marked reduction in the number of cilia.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi453 – 4531I → R: Decreased interaction with MAP4. No effect on interaction with IFT20.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625TRAF3-interacting protein 1PRO_0000299545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei316 – 3161PhosphoserineCombined sources
Modified residuei409 – 4091PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ149C2.
PaxDbiQ149C2.
PRIDEiQ149C2.

PTM databases

iPTMnetiQ149C2.

Expressioni

Gene expression databases

BgeeiQ149C2.
ExpressionAtlasiQ149C2. baseline and differential.
GenevisibleiQ149C2. MM.

Interactioni

Subunit structurei

Interacts with IL13RA1. Binds to microtubules, TRAF3 and DISC1 (By similarity). Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT88. Interacts with MAP4 (PubMed:26487268).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042391.

Structurei

Secondary structure

1
625
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Helixi24 – 285Combined sources
Helixi32 – 4615Combined sources
Turni48 – 514Combined sources
Turni55 – 584Combined sources
Helixi60 – 623Combined sources
Helixi66 – 8419Combined sources
Helixi92 – 965Combined sources
Helixi101 – 11616Combined sources
Helixi122 – 1309Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FMUX-ray1.59A/B/C/D1-133[»]
ProteinModelPortaliQ149C2.
SMRiQ149C2. Positions 1-133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 322322Abolishes microtubules binding when missingBy similarityAdd
BLAST
Regioni229 – 625397DISC1-interaction domainBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili472 – 600129Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi207 – 29690Arg-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRAF3IP1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3809. Eukaryota.
ENOG410ZHUT. LUCA.
GeneTreeiENSGT00720000108822.
HOGENOMiHOG000007159.
HOVERGENiHBG101388.
InParanoidiQ149C2.
KOiK19680.
OrthoDBiEOG7BKCV3.
PhylomeDBiQ149C2.
TreeFamiTF315473.

Family and domain databases

InterProiIPR018799. TRAF3IP1.
[Graphical view]
PANTHERiPTHR31363. PTHR31363. 2 hits.

Sequencei

Sequence statusi: Complete.

Q149C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAAVVRRTQ EALGKVIRRP PLTEKLLNKP PFRYLHDIIT EVIRITGFMK
60 70 80 90 100
GLYTDAEMKS ENVKDKDAKI SFLQKAIDVV MMVSGEPLAA KPARIVAGHE
110 120 130 140 150
PERTNELLQL IGKCCLSKLS SDEAVKRVLA GDKGDSRGRA QRTSKAQEPN
160 170 180 190 200
NKSGKEEESR IHKEDKRSSE AKERSASAEH KQKEELKEDS KPREKERDKE
210 220 230 240 250
KAKEADRDRH RDPDRDRNRD GEREKARARA KDRDRNNRDR DREAERDRER
260 270 280 290 300
DRRSEGGKEK ERVKDRDRDR DKGRDRERRK SKNGEHTRDP DREKSRDADK
310 320 330 340 350
PEKKSSSSGE ISRKLSDGSF KDVKAEMEAD ISVGASRSST LKPSKRRSKH
360 370 380 390 400
SLEGDSPSDA EVEAGPAGQD KPEVMENAEV PSELPSSLRR IPRPGSARPA
410 420 430 440 450
PPRVKRQEST ETLVVDRSGS GKTVSSVIID SQNSDNEDDE QFVVEAAPQL
460 470 480 490 500
SEIADIDMVP SGELEDEEKH GGLVKKILET KKDYEKLQQS LKPGEKERSL
510 520 530 540 550
IFESAWKKEK DIVSKEIEKL RVSIQTLCKS ALPLGKIMDY IQEDVDAMQN
560 570 580 590 600
ELQLWHSENR QHAEALSQEQ SITDSAVEPL KAELSELEQQ IRDQQDKICA
610 620
VKANILKNEE KIQKMVHSIN LSSRR
Length:625
Mass (Da):71,037
Last modified:September 11, 2007 - v2
Checksum:i2790471DAE271F7D
GO

Sequence cautioni

The sequence AAH46538.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521L → F in AAH46538 (PubMed:15489334).Curated
Sequence conflicti313 – 3131R → K in AAI17869 (PubMed:15489334).Curated
Sequence conflicti371 – 3711K → E in BAB29364 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY613437 mRNA. Translation: AAT72918.1.
BC046538 mRNA. Translation: AAH46538.1. Sequence problems.
BC117868 mRNA. Translation: AAI17869.1.
AK014457 mRNA. Translation: BAB29364.1.
CCDSiCCDS35664.1.
RefSeqiNP_082994.1. NM_028718.2.
UniGeneiMm.318430.
Mm.491399.

Genome annotation databases

EnsembliENSMUST00000047242; ENSMUSP00000042391; ENSMUSG00000034292.
GeneIDi74019.
KEGGimmu:74019.
UCSCiuc007cax.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY613437 mRNA. Translation: AAT72918.1.
BC046538 mRNA. Translation: AAH46538.1. Sequence problems.
BC117868 mRNA. Translation: AAI17869.1.
AK014457 mRNA. Translation: BAB29364.1.
CCDSiCCDS35664.1.
RefSeqiNP_082994.1. NM_028718.2.
UniGeneiMm.318430.
Mm.491399.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FMUX-ray1.59A/B/C/D1-133[»]
ProteinModelPortaliQ149C2.
SMRiQ149C2. Positions 1-133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042391.

PTM databases

iPTMnetiQ149C2.

Proteomic databases

MaxQBiQ149C2.
PaxDbiQ149C2.
PRIDEiQ149C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047242; ENSMUSP00000042391; ENSMUSG00000034292.
GeneIDi74019.
KEGGimmu:74019.
UCSCiuc007cax.2. mouse.

Organism-specific databases

CTDi26146.
MGIiMGI:1921269. Traf3ip1.

Phylogenomic databases

eggNOGiKOG3809. Eukaryota.
ENOG410ZHUT. LUCA.
GeneTreeiENSGT00720000108822.
HOGENOMiHOG000007159.
HOVERGENiHBG101388.
InParanoidiQ149C2.
KOiK19680.
OrthoDBiEOG7BKCV3.
PhylomeDBiQ149C2.
TreeFamiTF315473.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiTraf3ip1. mouse.
PROiQ149C2.
SOURCEiSearch...

Gene expression databases

BgeeiQ149C2.
ExpressionAtlasiQ149C2. baseline and differential.
GenevisibleiQ149C2. MM.

Family and domain databases

InterProiIPR018799. TRAF3IP1.
[Graphical view]
PANTHERiPTHR31363. PTHR31363. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mus musculus microtubule-interacting protein that associates with TRAF3."
    Ma B., He C., Lee C., Elias J.A.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRI.
    Tissue: Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 142-625.
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. Cited for: IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH IFT88, SUBCELLULAR LOCATION.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316 AND SER-409, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Testis.
  6. "Mutations in Traf3ip1 reveal defects in ciliogenesis, embryonic development, and altered cell size regulation."
    Berbari N.F., Kin N.W., Sharma N., Michaud E.J., Kesterson R.A., Yoder B.K.
    Dev. Biol. 360:66-76(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
  7. Cited for: FUNCTION, INTERACTION WITH MAP4, MUTAGENESIS OF ILE-453.

Entry informationi

Entry nameiMIPT3_MOUSE
AccessioniPrimary (citable) accession number: Q149C2
Secondary accession number(s): Q538H5, Q80VQ3, Q9CS98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 8, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.