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Protein

Nucleolar and coiled-body phosphoprotein 1

Gene

NOLC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).3 Publications

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GTP binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • RNA binding Source: UniProtKB
  • RNA polymerase I core binding Source: UniProtKB

GO - Biological processi

  • cell cycle Source: ProtInc
  • mitotic cell cycle Source: ProtInc
  • neural crest cell development Source: UniProtKB
  • neural crest formation Source: UniProtKB
  • nucleolus organization Source: Ensembl
  • regulation of translation Source: UniProtKB
  • rRNA processing Source: ProtInc

Keywordsi

LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

SIGNORiQ14978.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar and coiled-body phosphoprotein 1Curated
Alternative name(s):
140 kDa nucleolar phosphoprotein1 Publication
Short name:
Nopp1401 Publication
Hepatitis C virus NS5A-transactivated protein 13
Short name:
HCV NS5A-transactivated protein 13
Nucleolar 130 kDa protein1 Publication
Nucleolar phosphoprotein p1301 Publication
Gene namesi
Name:NOLC1Imported
Synonyms:KIAA00351 Publication, NS5ATP13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:15608. NOLC1.

Subcellular locationi

  • Nucleusnucleolus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.1 Publication

GO - Cellular componenti

  • Cajal body Source: Ensembl
  • cytoplasm Source: ProtInc
  • nucleolus Source: HPA

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9221.
MalaCardsiNOLC1.
OpenTargetsiENSG00000166197.
PharmGKBiPA31679.

Polymorphism and mutation databases

BioMutaiNOLC1.
DMDMi145559503.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969421 – 699Nucleolar and coiled-body phosphoprotein 1Add BLAST699

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-acetyllysineCombined sources1
Cross-linki67Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei87PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei188PhosphothreonineCombined sources1
Cross-linki193Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei362PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1
Modified residuei366PhosphoserineCombined sources1
Modified residuei397PhosphoserineCombined sources1
Modified residuei415N6-acetyllysine; alternateCombined sources1
Cross-linki415Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Modified residuei456PhosphoserineCombined sources1
Modified residuei508PhosphoserineCombined sources1
Modified residuei538PhosphoserineCombined sources1
Modified residuei563PhosphoserineCombined sources1
Cross-linki572Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Modified residuei580PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Cross-linki604Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei607PhosphothreonineCombined sources1
Modified residuei610PhosphothreonineCombined sources1
Modified residuei622PhosphoserineCombined sources1
Modified residuei643PhosphoserineCombined sources1
Modified residuei663N6-acetyllysineCombined sources1
Modified residuei683Omega-N-methylarginineCombined sources1
Modified residuei686PhosphoserineCombined sources1
Modified residuei698PhosphoserineCombined sources1

Post-translational modificationi

Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. NOLC1 is one of the mostly phosphorylated proteins in the cell.1 Publication
Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14978.
MaxQBiQ14978.
PaxDbiQ14978.
PeptideAtlasiQ14978.
PRIDEiQ14978.

PTM databases

iPTMnetiQ14978.
PhosphoSitePlusiQ14978.
SwissPalmiQ14978.

Expressioni

Gene expression databases

BgeeiENSG00000166197.
CleanExiHS_NOLC1.
ExpressionAtlasiQ14978. baseline and differential.
GenevisibleiQ14978. HS.

Organism-specific databases

HPAiCAB032654.
HPA037366.
HPA050388.

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with TCOF1 following monoubiquitination (PubMed:26399832). Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II (PubMed:10567578). Interacts with DKC1/NAP57, NOP58 and fibrillarin (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • RNA polymerase I core binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114654. 123 interactors.
IntActiQ14978. 46 interactors.
MINTiMINT-111465.
STRINGi9606.ENSP00000359024.

Structurei

3D structure databases

ProteinModelPortaliQ14978.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 42LisHPROSITE-ProRule annotationAdd BLAST33
Repeati84 – 95Acidic serine cluster 1Add BLAST12
Repeati125 – 136Acidic serine cluster 2Add BLAST12
Repeati167 – 178Acidic serine cluster 3Add BLAST12
Repeati221 – 232Acidic serine cluster 4Add BLAST12
Repeati264 – 275Acidic serine cluster 5Add BLAST12
Repeati325 – 336Acidic serine cluster 6Add BLAST12
Repeati363 – 375Acidic serine cluster 7Add BLAST13
Repeati425 – 436Acidic serine cluster 8Add BLAST12
Repeati470 – 481Acidic serine cluster 9Add BLAST12
Repeati519 – 529Acidic serine cluster 10Add BLAST11
Repeati555 – 566Acidic serine cluster 11Add BLAST12

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 56611 X 12 AA approximate repeats of an acidic serine clusterAdd BLAST483
Regioni204 – 382Interaction with RPA194Add BLAST179

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 82Nuclear localization signalSequence analysisAdd BLAST15
Motifi384 – 587Nuclear localization signalSequence analysisAdd BLAST204
Motifi601 – 617Nuclear localization signalSequence analysisAdd BLAST17

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2992. Eukaryota.
ENOG4111U5P. LUCA.
GeneTreeiENSGT00730000111092.
HOGENOMiHOG000113823.
HOVERGENiHBG075370.
InParanoidiQ14978.
OMAiFPKRKKG.
OrthoDBiEOG091G127U.
PhylomeDBiQ14978.
TreeFamiTF341730.

Family and domain databases

InterProiView protein in InterPro
IPR006594. LisH.
IPR007718. SRP40_C.
PfamiView protein in Pfam
PF05022. SRP40_C. 1 hit.
PROSITEiView protein in PROSITE
PS50896. LISH. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q14978-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADAGIRRVV PSDLYPLVLG FLRDNQLSEV ANKFAKATGA TQQDANASSL
60 70 80 90 100
LDIYSFWLKS AKVPERKLQA NGPVAKKAKK KASSSDSEDS SEEEEEVQGP
110 120 130 140 150
PAKKAAVPAK RVGLPPGKAA AKASESSSSE ESSDDDDEED QKKQPVQKGV
160 170 180 190 200
KPQAKAAKAP PKKAKSSDSD SDSSSEDEPP KNQKPKITPV TVKAQTKAPP
210 220 230 240 250
KPARAAPKIA NGKAASSSSS SSSSSSSDDS EEEKAAATPK KTVPKKQVVA
260 270 280 290 300
KAPVKAATTP TRKSSSSEDS SSDEEEEQKK PMKNKPGPYS SVPPPSAPPP
310 320 330 340 350
KKSLGTQPPK KAVEKQQPVE SSEDSSDESD SSSEEEKKPP TKAVVSKATT
360 370 380 390 400
KPPPAKKAAE SSSDSSDSDS SEDDEAPSKP AGTTKNSSNK PAVTTKSPAV
410 420 430 440 450
KPAAAPKQPV GGGQKLLTRK ADSSSSEEES SSSEEEKTKK MVATTKPKAT
460 470 480 490 500
AKAALSLPAK QAPQGSRDSS SDSDSSSSEE EEEKTSKSAV KKKPQKVAGG
510 520 530 540 550
AAPSKPASAK KGKAESSNSS SSDDSSEEEE EKLKGKGSPR PQAPKANGTS
560 570 580 590 600
ALTAQNGKAA KNSEEEEEEK KKAAVVVSKS GSLKKRKQNE AAKEAETPQA
610 620 630 640 650
KKIKLQTPNT FPKRKKGEKR ASSPFRRVRE EEIEVDSRVA DNSFDAKRGA
660 670 680 690
AGDWGERANQ VLKFTKGKSF RHEKTKKKRG SYRGGSISVQ VNSIKFDSE
Length:699
Mass (Da):73,603
Last modified:April 17, 2007 - v2
Checksum:i7C5C4F25677E2E61
GO
Isoform Beta (identifier: Q14978-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-241: K → KVWTITSVRAE

Show »
Length:709
Mass (Da):74,747
Checksum:i5A02742A231255AB
GO
Isoform 3 (identifier: Q14978-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-59: LK → LNR

Show »
Length:700
Mass (Da):73,745
Checksum:i5F8699563FBA24A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3D → A in BAA04803 (PubMed:7584026).Curated1
Sequence conflicti133S → R in CAA84063 (PubMed:7657714).Curated1
Sequence conflicti291 – 292SV → YA in CAA84063 (PubMed:7657714).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031677412G → V. Corresponds to variant dbSNP:rs11191224Ensembl.1
Natural variantiVAR_031678456S → P1 PublicationCorresponds to variant dbSNP:rs1049455Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03541558 – 59LK → LNR in isoform 3. 2 Publications2
Alternative sequenceiVSP_004338241K → KVWTITSVRAE in isoform Beta. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34289 mRNA. Translation: CAA84063.1.
AY820769 mRNA. Translation: AAV67777.1.
AL500527 Genomic DNA. Translation: CAH72220.1.
CH471066 Genomic DNA. Translation: EAW49714.1.
CH471066 Genomic DNA. Translation: EAW49715.1.
BC001883 mRNA. Translation: AAH01883.1.
D21262 mRNA. Translation: BAA04803.1.
CCDSiCCDS65925.1. [Q14978-3]
CCDS65926.1. [Q14978-2]
CCDS7530.1. [Q14978-1]
PIRiI38073.
RefSeqiNP_001271317.1. NM_001284388.1. [Q14978-2]
NP_001271318.1. NM_001284389.1. [Q14978-3]
NP_004732.2. NM_004741.4. [Q14978-1]
UniGeneiHs.523238.

Genome annotation databases

EnsembliENST00000405356; ENSP00000385410; ENSG00000166197. [Q14978-2]
ENST00000488254; ENSP00000475080; ENSG00000166197. [Q14978-3]
ENST00000605788; ENSP00000474710; ENSG00000166197. [Q14978-1]
GeneIDi9221.
KEGGihsa:9221.
UCSCiuc001kuo.4. human. [Q14978-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNOLC1_HUMAN
AccessioniPrimary (citable) accession number: Q14978
Secondary accession number(s): Q15030, Q5VV70, Q9BUV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: April 17, 2007
Last modified: July 5, 2017
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot