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Protein

Sodium/hydrogen exchanger 5

Gene

SLC9A5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction (By similarity).By similarity

GO - Molecular functioni

  • sodium:proton antiporter activity Source: ProtInc

GO - Biological processi

  • ion transport Source: Reactome
  • regulation of pH Source: InterPro
  • sodium ion transmembrane transport Source: GOC
  • transmembrane transport Source: Reactome
  • transport Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_19314. Sodium/Proton exchangers.

Protein family/group databases

TCDBi2.A.36.1.16. the monovalent cation:proton antiporter-1 (cpa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 5
Alternative name(s):
Na(+)/H(+) exchanger 5
Short name:
NHE-5
Solute carrier family 9 member 5
Gene namesi
Name:SLC9A5
Synonyms:NHE5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:11078. SLC9A5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei46 – 6621HelicalSequence AnalysisAdd
BLAST
Transmembranei74 – 9421HelicalSequence AnalysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence AnalysisAdd
BLAST
Transmembranei135 – 15521HelicalSequence AnalysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST
Transmembranei247 – 26721HelicalSequence AnalysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence AnalysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence AnalysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence AnalysisAdd
BLAST
Transmembranei399 – 41921HelicalSequence AnalysisAdd
BLAST
Transmembranei429 – 44921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35934.

Polymorphism and mutation databases

BioMutaiSLC9A5.
DMDMi12644417.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 896896Sodium/hydrogen exchanger 5PRO_0000052360Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Phosphorylated (Possible).

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ14940.
PRIDEiQ14940.

PTM databases

PhosphoSiteiQ14940.

Expressioni

Tissue specificityi

Expressed in brain, testis, spleen, and skeletal muscle.

Gene expression databases

BgeeiQ14940.
CleanExiHS_SLC9A5.
ExpressionAtlasiQ14940. baseline and differential.
GenevisibleiQ14940. HS.

Organism-specific databases

HPAiHPA041004.
HPA041329.

Interactioni

Subunit structurei

Interacts with CHP1 and CHP2 (By similarity). Interacts with ARRB2.By similarity1 Publication

Protein-protein interaction databases

BioGridi112442. 5 interactions.
IntActiQ14940. 1 interaction.
STRINGi9606.ENSP00000299798.

Structurei

3D structure databases

ProteinModelPortaliQ14940.
SMRiQ14940. Positions 68-454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiQ14940.
KOiK14723.
OMAiATNGKPP.
OrthoDBiEOG7KQ20Z.
PhylomeDBiQ14940.
TreeFamiTF317212.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018410. Na/H_exchanger_3/5.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01087. NAHEXCHNGR3.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q14940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRAALSLLA LPLAGAAEEP TQKPESPGEP PPGLELFRWQ WHEVEAPYLV
60 70 80 90 100
ALWILVASLA KIVFHLSRKV TSLVPESCLL ILLGLVLGGI VLAVAKKAEY
110 120 130 140 150
QLEPGTFFLF LLPPIVLDSG YFMPSRLFFD NLGAILTYAV VGTLWNAFTT
160 170 180 190 200
GAALWGLQQA GLVAPRVQAG LLDFLLFGSL ISAVDPVAVL AVFEEVHVNE
210 220 230 240 250
TLFIIVFGES LLNDAVTVVL YKVCNSFVEM GSANVQATDY LKGVASLFVV
260 270 280 290 300
SLGGAAVGLV FAFLLALTTR FTKRVRIIEP LLVFLLAYAA YLTAEMASLS
310 320 330 340 350
AILAVTMCGL GCKKYVEANI SHKSRTTVKY TMKTLASCAE TVIFMLLGIS
360 370 380 390 400
AVDSSKWAWD SGLVLGTLIF ILFFRALGVV LQTWVLNQFR LVPLDKIDQV
410 420 430 440 450
VMSYGGLRGA VAFALVILLD RTKVPAKDYF VATTIVVVFF TVIVQGLTIK
460 470 480 490 500
PLVKWLKVKR SEHHKPTLNQ ELHEHTFDHI LAAVEDVVGH HGYHYWRDRW
510 520 530 540 550
EQFDKKYLSQ LLMRRSAYRI RDQIWDVYYR LNIRDAISFV DQGGHVLSST
560 570 580 590 600
GLTLPSMPSR NSVAETSVTN LLRESGSGAC LDLQVIDTVR SGRDREDAVM
610 620 630 640 650
HHLLCGGLYK PRRRYKASCS RHFISEDAQE RQDKEVFQQN MKRRLESFKS
660 670 680 690 700
TKHNICFTKS KPRPRKTGRR KKDGVANAEA TNGKHRGLGF QDTAAVILTV
710 720 730 740 750
ESEEEEEESD SSETEKEDDE GIIFVARATS EVLQEGKVSG SLEVCPSPRI
760 770 780 790 800
IPPSPTCAEK ELPWKSGQGD LAVYVSSETT KIVPVDMQTG WNQSISSLES
810 820 830 840 850
LASPPCNQAP ILTCLPPHPR GTEEPQVPLH LPSDPRSSFA FPPSLAKAGR
860 870 880 890
SRSESSADLP QQQELQPLMG HKDHTHLSPG TATSHWCIQF NRGSRL
Length:896
Mass (Da):99,011
Last modified:December 1, 2000 - v2
Checksum:iB9D234BFE0922269
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021L → V in AAA87678 (PubMed:7759094).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111173 mRNA. Translation: AAC98696.1.
CH471092 Genomic DNA. Translation: EAW83114.1.
BC142671 mRNA. Translation: AAI42672.1.
U08607 Genomic DNA. Translation: AAA87678.1.
CCDSiCCDS42178.1.
RefSeqiNP_004585.1. NM_004594.2.
UniGeneiHs.439650.

Genome annotation databases

EnsembliENST00000299798; ENSP00000299798; ENSG00000135740.
GeneIDi6553.
KEGGihsa:6553.
UCSCiuc002esm.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111173 mRNA. Translation: AAC98696.1.
CH471092 Genomic DNA. Translation: EAW83114.1.
BC142671 mRNA. Translation: AAI42672.1.
U08607 Genomic DNA. Translation: AAA87678.1.
CCDSiCCDS42178.1.
RefSeqiNP_004585.1. NM_004594.2.
UniGeneiHs.439650.

3D structure databases

ProteinModelPortaliQ14940.
SMRiQ14940. Positions 68-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112442. 5 interactions.
IntActiQ14940. 1 interaction.
STRINGi9606.ENSP00000299798.

Chemistry

BindingDBiQ14940.
ChEMBLiCHEMBL3058.

Protein family/group databases

TCDBi2.A.36.1.16. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSiteiQ14940.

Polymorphism and mutation databases

BioMutaiSLC9A5.
DMDMi12644417.

Proteomic databases

PaxDbiQ14940.
PRIDEiQ14940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299798; ENSP00000299798; ENSG00000135740.
GeneIDi6553.
KEGGihsa:6553.
UCSCiuc002esm.3. human.

Organism-specific databases

CTDi6553.
GeneCardsiGC16P067282.
HGNCiHGNC:11078. SLC9A5.
HPAiHPA041004.
HPA041329.
MIMi600477. gene.
neXtProtiNX_Q14940.
PharmGKBiPA35934.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiQ14940.
KOiK14723.
OMAiATNGKPP.
OrthoDBiEOG7KQ20Z.
PhylomeDBiQ14940.
TreeFamiTF317212.

Enzyme and pathway databases

ReactomeiREACT_19314. Sodium/Proton exchangers.

Miscellaneous databases

GeneWikiiSLC9A5.
GenomeRNAii6553.
NextBioi25499.
PROiQ14940.
SOURCEiSearch...

Gene expression databases

BgeeiQ14940.
CleanExiHS_SLC9A5.
ExpressionAtlasiQ14940. baseline and differential.
GenevisibleiQ14940. HS.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR018410. Na/H_exchanger_3/5.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01087. NAHEXCHNGR3.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, genomic organization, and functional expression of Na+/H+ exchanger isoform 5 (NHE5) from human brain."
    Baird N.R., Orlowski J., Szabo E.Z., Zaun H.C., Schultheis P.J., Menon A.G., Shull G.E.
    J. Biol. Chem. 274:4377-4382(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Molecular cloning and physical and genetic mapping of a novel human Na+/H+ exchanger (NHE5/SLC9A5) to chromosome 16q22.1."
    Klanke C.A., Su Y.R., Callen D.F., Wang Z., Meneton P., Baird N., Kandasamy R.A., Orlowski J., Otterud B.E., Leppert M., Shull G.E., Menon A.G.
    Genomics 25:615-622(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-218.
  5. "beta-Arrestins bind and decrease cell-surface abundance of the Na+/H+ exchanger NHE5 isoform."
    Szabo E.Z., Numata M., Lukashova V., Iannuzzi P., Orlowski J.
    Proc. Natl. Acad. Sci. U.S.A. 102:2790-2795(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARRB2.

Entry informationi

Entry nameiSL9A5_HUMAN
AccessioniPrimary (citable) accession number: Q14940
Secondary accession number(s): A5PKY7, Q9Y626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: June 24, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

The number, localization and denomination of hydrophobic domains in the Na+/H+ exchangers vary among authors.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.