Reviewed,
UniProtKB/Swiss-Prot Q14934 (NFAC4_HUMAN)
Last modified
November 25, 2008.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nuclear factor of activated T-cells, cytoplasmic 4 Short name=NF-ATc4 Short name=NFATc4 Alternative name(s): T-cell transcription factor NFAT3 Short name=NF-AT3 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 902 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4 By similarity. |
| Subunit structure | Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. |
| Subcellular location | Cytoplasm. Nucleus. Note= Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC play a key role in the gene transcription. |
| Tissue specificity | Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus. |
| Domain | Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors By similarity. |
| Post-translational modification | Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin By similarity. |
| Sequence similarities | Contains 1 RHD (Rel-like) domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Cytoplasm Nucleus |
| Coding sequence diversity | Polymorphism |
| Domain | Repeat |
| Ligand | DNA-binding |
| Molecular function | Activator |
| PTM | Phosphoprotein |
| Technical term | 3D-structure |
Gene Ontology (GO) | |
| Biological process | inflammatory response Ref.1 Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependentInferred from electronic annotation. Source: InterPro transcription from RNA polymerase II promoter Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nucleusInferred from electronic annotation. Source: InterPro |
| Molecular function | transcription coactivator activity Ref.1 Traceable author statement. Source: ProtInc transcription factor activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 902 | 902 | Nuclear factor of activated T-cells, cytoplasmic 4 | PRO_0000205182 | |||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||
| Repeat | 213 – 229 | 17 | SP 1 | ||||||||||||||||||||||||
| Repeat | 277 – 293 | 17 | SP 2; approximate | ||||||||||||||||||||||||
| Domain | 401 – 582 | 182 | RHD | ||||||||||||||||||||||||
| DNA binding | 430 – 437 | 8 | |||||||||||||||||||||||||
| Region | 114 – 119 | 6 | Calcineurin-binding | ||||||||||||||||||||||||
| Region | 213 – 293 | 81 | 2 approximate SP repeats | ||||||||||||||||||||||||
| Motif | 268 – 270 | 3 | Nuclear localization signal | ||||||||||||||||||||||||
| Motif | 672 – 674 | 3 | Nuclear localization signal | ||||||||||||||||||||||||
| Compositional bias | 62 – 69 | 8 | Poly-Pro | ||||||||||||||||||||||||
| Compositional bias | 297 – 304 | 8 | Poly-Pro | ||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||
| Modified residue | 272 | 1 | Phosphoserine | ||||||||||||||||||||||||
| Modified residue | 289 | 1 | Phosphoserine | ||||||||||||||||||||||||
| Modified residue | 334 | 1 | Phosphoserine | ||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||
| Natural variant | 160 | 1 | G → A: dbSNP rs2229309. | VAR_046985 | |||||||||||||||||||||||
| Natural variant | 246 | 1 | S → N: dbSNP rs2228231. | VAR_046986 | |||||||||||||||||||||||
| Natural variant | 800 | 1 | S → P: dbSNP rs7149586. | VAR_046987 | |||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||
| Beta strand | 587 – 594 | 8 | |||||||||||||||||||||||||
| Beta strand | 602 – 610 | 9 | |||||||||||||||||||||||||
| Beta strand | 616 – 620 | 5 | |||||||||||||||||||||||||
| Turn | 638 – 640 | 3 | |||||||||||||||||||||||||
| Beta strand | 645 – 649 | 5 | |||||||||||||||||||||||||
| Beta strand | 662 – 669 | 8 | |||||||||||||||||||||||||
| Beta strand | 671 – 673 | 3 | |||||||||||||||||||||||||
| Beta strand | 679 – 684 | 6 | |||||||||||||||||||||||||
| Beta strand | 687 – 689 | 3 | |||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins." Hoey T., Sun Y.-L., Williamson K., Xu X. Immunity 2:461-472(1995) [PubMed: 7749981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ALA-160 AND PRO-800. Tissue: T-cell. |
| [2] | "Alternative splicing and expression of human and mouse NFAT genes." Vihma H., Pruunsild P., Timmusk T. Genomics 0:0-0(2008) [PubMed: 18675896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary. |
| [4] | "Generic signals and specific outcomes: signaling through Ca2+, calcineurin, and NF-AT." Crabtree G.R. Cell 96:611-614(1999) [PubMed: 10089876] [Abstract] Cited for: REVIEW. |
| [5] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272 AND SER-334, MASS SPECTROMETRY. |
| [7] | "Solution structure of the TIG domain from human nuclear factor of activated T-cells, cytoplasmic 4." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 585-691. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L41066 mRNA. Translation: AAA79175.1. EU887641 mRNA. Translation: ACG55661.1. BC053855 mRNA. Translation: AAH53855.1. | |||||||||||||
| UniGene | Hs.77810 | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q14934. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSG00000100968. Homo sapiens. [Contig view] | ||||||||||||
| KEGG | hsa:4776. | ||||||||||||
Organism-specific databases | |||||||||||||
| HGNC | HGNC:7778. NFATC4. | ||||||||||||
| MIM | 602699. gene. | ||||||||||||
| PharmGKB | PA31584. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
| GeneCards | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q14934. | ||||||||||||
| HOVERGEN | Q14934. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q14934. | ||||||||||||
| CleanEx | HS_NFATC4. | ||||||||||||
| GermOnline | ENSG00000100968. Homo sapiens. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR013783. Ig-like_fold. IPR002909. IPT_TIG_rcpt. IPR000451. NF_Rel_dor. IPR008366. NFAT. IPR011539. RHD. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:2.60.40.10. Ig-like_fold. 1 hit. G3DSA:2.60.40.340. RHD. 1 hit. | ||||||||||||
| PANTHER | PTHR12533. NFAT. 1 hit. | ||||||||||||
| Pfam | PF00554. RHD. 1 hit. PF01833. TIG. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR01789. NUCFACTORATC. | ||||||||||||
| SMART | SM00429. IPT. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS01204. REL_1. False negative. PS50254. REL_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | NFAC4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q14934 Secondary accession number(s): Q7Z598 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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