Q148G2 (G6PC3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-6-phosphatase 3
Short name=G-6-Pase 3
Short name=G6Pase 3
EC=3.1.3.9

Gene names

Name:

G6PC3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function By similarity.
Catalytic activity	D-glucose 6-phosphate + H2O = D-glucose + phosphate.
Enzyme regulation	Inhibited by vanadate By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-6-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Glucose-6-phosphatase 3		PRO_0000334511
Regions
	Topological domain	1 – 24	24	Lumenal Potential
	Transmembrane	25 – 45	21	Helical; Potential
	Topological domain	46 – 54	9	Cytoplasmic Potential
	Transmembrane	55 – 75	21	Helical; Potential
	Topological domain	76 – 108	33	Lumenal Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Topological domain	130 – 140	11	Cytoplasmic Potential
	Transmembrane	141 – 162	22	Helical; Potential
	Topological domain	163 – 167	5	Lumenal Potential
	Transmembrane	168 – 186	19	Helical; Potential
	Topological domain	187 – 197	11	Cytoplasmic Potential
	Transmembrane	198 – 218	21	Helical; Potential
	Topological domain	219 – 254	36	Lumenal Potential
	Transmembrane	255 – 273	19	Helical; Potential
	Topological domain	274 – 283	10	Cytoplasmic Potential
	Transmembrane	284 – 304	21	Helical; Potential
	Topological domain	305 – 307	3	Lumenal Potential
	Transmembrane	308 – 328	21	Helical; Potential
	Topological domain	329 – 346	18	Cytoplasmic Potential
Sites
	Active site	114	1	Proton donor Potential
	Active site	167	1	Nucleophile By similarity
	Binding site	79	1	Substrate Potential
	Binding site	161	1	Substrate Potential
Experimental info
	Sequence conflict	239	1	E → G in AAP40635. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q148G2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: EBFB51839598E363	FASTA	346	38,742
	10 20 30 40 50 60 MESTLGAGIA MAEALQNQLP WLENVWLWVT FLGDPKSLFL FYFPAAYYAS RRVGIAVLWI 70 80 90 100 110 120 SLITEWLNLV FKWFLFGDRP FWWVHESGYY SQAPAQVHQF PSSCETGPGS PSGHCMITGA 130 140 150 160 170 180 ALWPIMTAVS SQMATRAHSR WVRVIPSLAY CTFLLAVGLS RVFLLAHFPH QVLAGLITGA 190 200 210 220 230 240 VLGWLMTPQV PMERELSFYG LTSLALLLGA SLIYWTLFTL GLDLSWSINL ASKWCERPEW 250 260 270 280 290 300 VHLDSRPFAS LSRDSGAALG LGIALHSPCY AQVRRAHLGY GQKLVCLVLA MGLLGPLNWL 310 320 330 340 GYPPQISLFY IFNFLKYTLW PCLVLALVPW LVHMFSAQEA PPIRSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Clottes E., Mounier R., Rossignol F., Bonnefont J., Guionie O., Burchell A. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal cerebellum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY279358 mRNA. Translation: AAP40635.1. BC118357 mRNA. Translation: AAI18358.1.
IPI	IPI00696280.
RefSeq	NP_899208.2. NM_183364.2.
UniGene	Bt.23154.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q148G2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000021621; ENSBTAP00000021621; ENSBTAG00000016253.
GeneID	369023.
KEGG	bta:369023.
Organism-specific databases
CTD	92579.
Phylogenomic databases
eggNOG	maNOG13025.
GeneTree	ENSGT00510000046465.
HOVERGEN	HBG003560.
InParanoid	Q148G2.
OMA	TFLGDPK.
OrthoDB	EOG441QCC.
PhylomeDB	Q148G2.
Family and domain databases
InterPro	IPR016275. Glucose-6-phosphatase. IPR016118. P_Acid_Pase/Cl_peroxidase_N. IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Gene3D	G3DSA:1.20.144.10. P_Acid_Pase/Cl_peroxidase_N. 1 hit.
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
PIRSF	PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
SUPFAM	SSF48317. AcPase_VanPerase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G6PC3_BOVIN

Accession

Primary (citable) accession number: Q148G2
Secondary accession number(s): Q7YSF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 22, 2006
Last modified:	September 21, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program