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Protein

LIM and SH3 domain protein 1

Gene

LASP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).By similarity

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • ion transmembrane transporter activity Source: UniProtKB
  • SH3/SH2 adaptor activity Source: ProtInc
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000002834-MONOMER.
SIGNORiQ14847.

Names & Taxonomyi

Protein namesi
Recommended name:
LIM and SH3 domain protein 1
Short name:
LASP-1
Alternative name(s):
Metastatic lymph node gene 50 protein
Short name:
MLN 50
Gene namesi
Name:LASP1
Synonyms:MLN50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:6513. LASP1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cortical actin cytoskeleton Source: UniProtKB
  • cytoplasm Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi3927.
OpenTargetsiENSG00000002834.
PharmGKBiPA30298.

Polymorphism and mutation databases

DMDMi3122342.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757611 – 261LIM and SH3 domain protein 1Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei42N6-acetyllysineCombined sources1
Modified residuei68PhosphothreonineCombined sources1
Modified residuei75N6-methyllysineCombined sources1
Modified residuei99PhosphoserineCombined sources1
Modified residuei104PhosphothreonineCombined sources1
Modified residuei112N6-succinyllysineBy similarity1
Modified residuei118PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ14847.
MaxQBiQ14847.
PaxDbiQ14847.
PeptideAtlasiQ14847.
PRIDEiQ14847.

2D gel databases

OGPiQ14847.
SWISS-2DPAGEQ14847.

PTM databases

iPTMnetiQ14847.
PhosphoSitePlusiQ14847.
SwissPalmiQ14847.

Expressioni

Gene expression databases

BgeeiENSG00000002834.
CleanExiHS_LASP1.
ExpressionAtlasiQ14847. baseline and differential.
GenevisibleiQ14847. HS.

Organism-specific databases

HPAiCAB022049.
HPA012072.

Interactioni

Subunit structurei

Interacts with F-actin (By similarity). Interacts with ANKRD54 (By similarity). Interacts with KBTBD10.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGEF15D3DTR73EBI-742828,EBI-10176602
FXR2P511163EBI-742828,EBI-740459
GOLGA2Q083793EBI-742828,EBI-618309
KRTAP4-2Q9BYR53EBI-742828,EBI-10172511
LPPQ930523EBI-742828,EBI-718388
LZTS2Q9BRK43EBI-742828,EBI-741037
MDFIQ997503EBI-742828,EBI-724076
PSMA3P257883EBI-742828,EBI-348380
RBPMSQ93062-34EBI-9088686,EBI-740343
RELQ048643EBI-742828,EBI-307352
RHOXF2Q9BQY43EBI-742828,EBI-372094
SEPT3Q9UH033EBI-742828,EBI-727037
SH2D2AQ9NP312EBI-742828,EBI-490630
SPRY2O435973EBI-742828,EBI-742487
TCF4P158843EBI-742828,EBI-533224
THAP1Q9NVV93EBI-742828,EBI-741515
TJP2Q9UDY29EBI-742828,EBI-1042602
TRIM27P143733EBI-742828,EBI-719493
TRIP13Q156457EBI-742828,EBI-358993
ZBTB9Q96C003EBI-742828,EBI-395708
ZC2HC1AQ96GY03EBI-742828,EBI-5458880
ZDHHC17Q8IUH53EBI-9088686,EBI-524753
ZYXQ159425EBI-742828,EBI-444225

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi110120. 64 interactors.
IntActiQ14847. 50 interactors.
MINTiMINT-5001286.
STRINGi9606.ENSP00000325240.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi207 – 211Combined sources5
Beta strandi228 – 236Combined sources9
Beta strandi239 – 244Combined sources6
Turni245 – 248Combined sources4
Beta strandi249 – 254Combined sources6
Helixi255 – 257Combined sources3
Beta strandi258 – 260Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I35X-ray1.40A202-261[»]
ProteinModelPortaliQ14847.
SMRiQ14847.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14847.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 56LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST52
Repeati61 – 95Nebulin 1Add BLAST35
Repeati97 – 131Nebulin 2Add BLAST35
Domaini202 – 261SH3PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi201 – 204Poly-Gly4

Sequence similaritiesi

Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation
Contains 2 nebulin repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1702. Eukaryota.
ENOG4111GQ8. LUCA.
GeneTreeiENSGT00530000062924.
HOGENOMiHOG000006616.
HOVERGENiHBG054636.
InParanoidiQ14847.
OMAiYGYKEPA.
OrthoDBiEOG091G0U2O.
PhylomeDBiQ14847.
TreeFamiTF319104.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14847-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPNCARCGK IVYPTEKVNC LDKFWHKACF HCETCKMTLN MKNYKGYEKK
60 70 80 90 100
PYCNAHYPKQ SFTMVADTPE NLRLKQQSEL QSQVRYKEEF EKNKGKGFSV
110 120 130 140 150
VADTPELQRI KKTQDQISNI KYHEEFEKSR MGPSGGEGME PERRDSQDGS
160 170 180 190 200
SYRRPLEQQQ PHHIPTSAPV YQQPQQQPVA QSYGGYKEPA APVSIQRSAP
210 220 230 240 250
GGGGKRYRAV YDYSAADEDE VSFQDGDTIV NVQQIDDGWM YGTVERTGDT
260
GMLPANYVEA I
Length:261
Mass (Da):29,717
Last modified:January 1, 1998 - v2
Checksum:i3B89B988605B3639
GO
Isoform 2 (identifier: Q14847-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-261: GGGGKRYRAV...MLPANYVEAI → ICLQHIPRHR...LTYPHIPGLG

Note: No experimental confirmation available.
Show »
Length:323
Mass (Da):36,014
Checksum:iB6A28D1D97AB2C69
GO
Isoform 3 (identifier: Q14847-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: MNPNCARCGK...LKQQSELQSQ → MLPLRDLQDDTEHEELQGLREEALLQR

Note: No experimental confirmation available.
Show »
Length:205
Mass (Da):23,181
Checksum:i4CD6D2622F74CCF4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79E → R in AAH12460 (PubMed:15489334).Curated1
Sequence conflicti210V → A in AAH12460 (PubMed:15489334).Curated1
Sequence conflicti220E → A in AAH12460 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546111 – 83MNPNC…ELQSQ → MLPLRDLQDDTEHEELQGLR EEALLQR in isoform 3. 1 PublicationAdd BLAST83
Alternative sequenceiVSP_016554201 – 261GGGGK…YVEAI → ICLQHIPRHRIRPGRDPSIL QCLCFLKPATACDSYPSSSF FCQLKPSSATSAGSLLWQAS PLIDFLVFSLDGTGMGLSGG GRGPWGRAGMGDLLACGPHL PLCSLPSHPPAQLLTYPHIP GLG in isoform 2. 1 PublicationAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82456 mRNA. Translation: CAA57833.1.
AK294704 mRNA. Translation: BAG57861.1.
AC006441 Genomic DNA. No translation available.
AC110749 Genomic DNA. No translation available.
BC007560 mRNA. Translation: AAH07560.1.
BC012460 mRNA. Translation: AAH12460.1.
CCDSiCCDS11331.1. [Q14847-1]
CCDS62164.1. [Q14847-3]
PIRiS68234.
RefSeqiNP_001258537.1. NM_001271608.1. [Q14847-3]
NP_006139.1. NM_006148.3. [Q14847-1]
UniGeneiHs.741156.

Genome annotation databases

EnsembliENST00000318008; ENSP00000325240; ENSG00000002834. [Q14847-1]
ENST00000433206; ENSP00000401048; ENSG00000002834. [Q14847-3]
ENST00000435347; ENSP00000392853; ENSG00000002834. [Q14847-1]
GeneIDi3927.
KEGGihsa:3927.
UCSCiuc002hra.3. human. [Q14847-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82456 mRNA. Translation: CAA57833.1.
AK294704 mRNA. Translation: BAG57861.1.
AC006441 Genomic DNA. No translation available.
AC110749 Genomic DNA. No translation available.
BC007560 mRNA. Translation: AAH07560.1.
BC012460 mRNA. Translation: AAH12460.1.
CCDSiCCDS11331.1. [Q14847-1]
CCDS62164.1. [Q14847-3]
PIRiS68234.
RefSeqiNP_001258537.1. NM_001271608.1. [Q14847-3]
NP_006139.1. NM_006148.3. [Q14847-1]
UniGeneiHs.741156.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I35X-ray1.40A202-261[»]
ProteinModelPortaliQ14847.
SMRiQ14847.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110120. 64 interactors.
IntActiQ14847. 50 interactors.
MINTiMINT-5001286.
STRINGi9606.ENSP00000325240.

PTM databases

iPTMnetiQ14847.
PhosphoSitePlusiQ14847.
SwissPalmiQ14847.

Polymorphism and mutation databases

DMDMi3122342.

2D gel databases

OGPiQ14847.
SWISS-2DPAGEQ14847.

Proteomic databases

EPDiQ14847.
MaxQBiQ14847.
PaxDbiQ14847.
PeptideAtlasiQ14847.
PRIDEiQ14847.

Protocols and materials databases

DNASUi3927.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318008; ENSP00000325240; ENSG00000002834. [Q14847-1]
ENST00000433206; ENSP00000401048; ENSG00000002834. [Q14847-3]
ENST00000435347; ENSP00000392853; ENSG00000002834. [Q14847-1]
GeneIDi3927.
KEGGihsa:3927.
UCSCiuc002hra.3. human. [Q14847-1]

Organism-specific databases

CTDi3927.
DisGeNETi3927.
GeneCardsiLASP1.
HGNCiHGNC:6513. LASP1.
HPAiCAB022049.
HPA012072.
MIMi602920. gene.
neXtProtiNX_Q14847.
OpenTargetsiENSG00000002834.
PharmGKBiPA30298.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1702. Eukaryota.
ENOG4111GQ8. LUCA.
GeneTreeiENSGT00530000062924.
HOGENOMiHOG000006616.
HOVERGENiHBG054636.
InParanoidiQ14847.
OMAiYGYKEPA.
OrthoDBiEOG091G0U2O.
PhylomeDBiQ14847.
TreeFamiTF319104.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000002834-MONOMER.
SIGNORiQ14847.

Miscellaneous databases

ChiTaRSiLASP1. human.
EvolutionaryTraceiQ14847.
GeneWikiiLASP1.
GenomeRNAii3927.
PROiQ14847.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000002834.
CleanExiHS_LASP1.
ExpressionAtlasiQ14847. baseline and differential.
GenevisibleiQ14847. HS.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLASP1_HUMAN
AccessioniPrimary (citable) accession number: Q14847
Secondary accession number(s): B4DGQ0, Q96ED2, Q96IG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.