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Protein

Kinesin-like protein KIF22

Gene

KIF22

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed:25743205).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 134ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: ProtInc
  • microtubule motor activity Source: ProtInc

GO - Biological processi

  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  • DNA repair Source: InterPro
  • metaphase plate congression Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • mitotic metaphase plate congression Source: UniProtKB
  • mitotic nuclear division Source: ProtInc
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
  • sister chromatid cohesion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000079616-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.
SignaLinkiQ14807.
SIGNORiQ14807.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF22
Alternative name(s):
Kinesin-like DNA-binding protein
Kinesin-like protein 4
Gene namesi
Name:KIF22
Synonyms:KID, KNSL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:6391. KIF22.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: Ensembl
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • focal adhesion Source: HPA
  • kinesin complex Source: GO_Central
  • kinetochore Source: ProtInc
  • microtubule Source: UniProtKB-KW
  • mitotic spindle Source: UniProtKB
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Involvement in diseasei

Spondyloepimetaphyseal dysplasia with joint laxity, 2 (SEMDJL2)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA bone disease characterized by short stature, distinctive midface retrusion, progressive knee malalignment (genu valgum and/or varum), generalized ligamentous laxity, and mild spinal deformity. Intellectual development is not impaired. Radiographic characteristics include significantly retarded epiphyseal ossification that evolves into epiphyseal dysplasia and precocious osteoarthritis, metaphyseal irregularities and vertical striations, constricted femoral neck, slender metacarpals and metatarsals, and mild thoracolumbar kyphosis or scoliosis with normal or mild platyspondyly. The most distinctive features for differential diagnosis of SEMDJL2 are the slender metacarpals and phalanges and the progressive degeneration of carpal bones; however, these 2 features are evident only in older children and young adults. The soft consistency of cartilage in the airways leads to laryngotracheomalacia with proneness to respiratory obstruction and inspiratory stridor in infancy and childhood.
See also OMIM:603546
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_067345148P → L in SEMDJL2. 2 PublicationsCorresponds to variant rs193922921dbSNPEnsembl.1
Natural variantiVAR_067346148P → S in SEMDJL2. 1 PublicationCorresponds to variant rs193922920dbSNPEnsembl.1
Natural variantiVAR_067347149R → L in SEMDJL2. 1 PublicationCorresponds to variant rs193922922dbSNPEnsembl.1
Natural variantiVAR_067348149R → Q in SEMDJL2. 2 PublicationsCorresponds to variant rs193922922dbSNPEnsembl.1

Keywords - Diseasei

Disease mutation, Dwarfism

Organism-specific databases

DisGeNETi3835.
MalaCardsiKIF22.
MIMi603546. phenotype.
OpenTargetsiENSG00000079616.
Orphaneti93360. Spondyloepimetaphyseal dysplasia with multiple dislocations.
PharmGKBiPA30180.

Chemistry databases

ChEMBLiCHEMBL5470.

Polymorphism and mutation databases

BioMutaiKIF22.
DMDMi19863381.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001254331 – 665Kinesin-like protein KIF22Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei412PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1
Modified residuei452PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1
Modified residuei581PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14807.
MaxQBiQ14807.
PaxDbiQ14807.
PeptideAtlasiQ14807.
PRIDEiQ14807.

PTM databases

iPTMnetiQ14807.
PhosphoSitePlusiQ14807.

Expressioni

Tissue specificityi

Expressed in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes.1 Publication

Gene expression databases

BgeeiENSG00000079616.
CleanExiHS_KIF22.
ExpressionAtlasiQ14807. baseline and differential.
GenevisibleiQ14807. HS.

Organism-specific databases

HPAiHPA041076.

Interactioni

Subunit structurei

Interacts with FAM83D (PubMed:18485706). Interacts with SIAH1 (PubMed:11146551).2 Publications

Protein-protein interaction databases

BioGridi110033. 59 interactors.
IntActiQ14807. 46 interactors.
MINTiMINT-156095.
STRINGi9606.ENSP00000160827.

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi45 – 50Combined sources6
Beta strandi85 – 89Combined sources5
Beta strandi91 – 94Combined sources4
Helixi100 – 107Combined sources8
Helixi109 – 111Combined sources3
Helixi112 – 115Combined sources4
Turni116 – 118Combined sources3
Beta strandi121 – 127Combined sources7
Helixi133 – 137Combined sources5
Beta strandi141 – 144Combined sources4
Helixi146 – 161Combined sources16
Beta strandi167 – 180Combined sources14
Beta strandi183 – 189Combined sources7
Helixi218 – 228Combined sources11
Helixi243 – 245Combined sources3
Beta strandi246 – 260Combined sources15
Beta strandi265 – 274Combined sources10
Helixi299 – 312Combined sources14
Helixi320 – 322Combined sources3
Helixi324 – 328Combined sources5
Turni329 – 331Combined sources3
Beta strandi332 – 334Combined sources3
Beta strandi338 – 345Combined sources8
Helixi349 – 351Combined sources3
Helixi352 – 362Combined sources11
Beta strandi364 – 369Combined sources6
Turni577 – 579Combined sources3
Helixi582 – 598Combined sources17
Helixi601 – 606Combined sources6
Helixi612 – 625Combined sources14
Helixi631 – 636Combined sources6
Helixi642 – 658Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EDUNMR-A570-660[»]
3BFNX-ray2.30A40-400[»]
ProteinModelPortaliQ14807.
SMRiQ14807.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14807.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 368Kinesin motorPROSITE-ProRule annotationAdd BLAST326

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili465 – 508Sequence analysisAdd BLAST44

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0242. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOGENOMiHOG000007569.
HOVERGENiHBG052252.
InParanoidiQ14807.
KOiK10403.
OMAiAGQRCGP.
OrthoDBiEOG091G0FFB.
PhylomeDBiQ14807.
TreeFamiTF105233.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR010994. RuvA_2-like.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00278. HhH1. 2 hits.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14807-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGGSTQQR RREMAAASAA AISGAGRCRL SKIGATRRPP PARVRVAVRL
60 70 80 90 100
RPFVDGTAGA SDPPCVRGMD SCSLEIANWR NHQETLKYQF DAFYGERSTQ
110 120 130 140 150
QDIYAGSVQP ILRHLLEGQN ASVLAYGPTG AGKTHTMLGS PEQPGVIPRA
160 170 180 190 200
LMDLLQLTRE EGAEGRPWAL SVTMSYLEIY QEKVLDLLDP ASGDLVIRED
210 220 230 240 250
CRGNILIPGL SQKPISSFAD FERHFLPASR NRTVGATRLN QRSSRSHAVL
260 270 280 290 300
LVKVDQRERL APFRQREGKL YLIDLAGSED NRRTGNKGLR LKESGAINTS
310 320 330 340 350
LFVLGKVVDA LNQGLPRVPY RDSKLTRLLQ DSLGGSAHSI LIANIAPERR
360 370 380 390 400
FYLDTVSALN FAARSKEVIN RPFTNESLQP HALGPVKLSQ KELLGPPEAK
410 420 430 440 450
RARGPEEEEI GSPEPMAAPA SASQKLSPLQ KLSSMDPAML ERLLSLDRLL
460 470 480 490 500
ASQGSQGAPL LSTPKRERMV LMKTVEEKDL EIERLKTKQK ELEAKMLAQK
510 520 530 540 550
AEEKENHCPT MLRPLSHRTV TGAKPLKKAV VMPLQLIQEQ AASPNAEIHI
560 570 580 590 600
LKNKGRKRKL ESLDALEPEE KAEDCWELQI SPELLAHGRQ KILDLLNEGS
610 620 630 640 650
ARDLRSLQRI GPKKAQLIVG WRELHGPFSQ VEDLERVEGI TGKQMESFLK
660
ANILGLAAGQ RCGAS
Length:665
Mass (Da):73,262
Last modified:September 26, 2001 - v5
Checksum:iC6C0AC96741DD387
GO
Isoform 2 (identifier: Q14807-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: No experimental confirmation available.
Show »
Length:597
Mass (Da):66,307
Checksum:i4B6D0F60A2426335
GO

Sequence cautioni

The sequence AAC08709 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence EAW80007 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24Missing in BAA33063 (PubMed:9790757).Curated1
Sequence conflicti122S → KV in BAA33063 (PubMed:9790757).Curated1
Sequence conflicti135 – 169HTMLG…GRPWA → THAGQPRATWGDPAGSHGPP AAHKGGGCRGPAMG (PubMed:9790757).CuratedAdd BLAST35
Sequence conflicti216S → N in BAD97151 (Ref. 5) Curated1
Sequence conflicti270L → P in BAG35167 (PubMed:14702039).Curated1
Sequence conflicti303V → A in BAA33063 (PubMed:9790757).Curated1
Sequence conflicti381H → R in BAD97151 (Ref. 5) Curated1
Sequence conflicti418 – 456APASA…SQGSQ → SSSLCLPETQPPTEAKAAWT RPCGAPPQLGPSACLPGEP in BAA33063 (PubMed:9790757).CuratedAdd BLAST39
Sequence conflicti505 – 513ENHCPTMLR → RTIVPQCSG in BAA33063 (PubMed:9790757).Curated9

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_067345148P → L in SEMDJL2. 2 PublicationsCorresponds to variant rs193922921dbSNPEnsembl.1
Natural variantiVAR_067346148P → S in SEMDJL2. 1 PublicationCorresponds to variant rs193922920dbSNPEnsembl.1
Natural variantiVAR_067347149R → L in SEMDJL2. 1 PublicationCorresponds to variant rs193922922dbSNPEnsembl.1
Natural variantiVAR_067348149R → Q in SEMDJL2. 2 PublicationsCorresponds to variant rs193922922dbSNPEnsembl.1
Natural variantiVAR_067349232R → Q.1 PublicationCorresponds to variant rs201659270dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0464281 – 68Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017430 mRNA. Translation: BAA33019.2.
AB017335 Genomic DNA. Translation: BAA33063.1.
BT007259 mRNA. Translation: AAP35923.1.
AK294380 mRNA. Translation: BAH11751.1.
AK312234 mRNA. Translation: BAG35167.1.
AK316389 mRNA. Translation: BAH14760.1.
AK223431 mRNA. Translation: BAD97151.1.
AC002301 Genomic DNA. Translation: AAC08709.1. Sequence problems.
AC009133 Genomic DNA. No translation available.
CH471238 Genomic DNA. Translation: EAW80005.1.
CH471238 Genomic DNA. Translation: EAW80007.1. Sequence problems.
BC004352 mRNA. Translation: AAH04352.1.
BC028155 mRNA. Translation: AAH28155.1.
CCDSiCCDS10653.1. [Q14807-1]
CCDS58444.1. [Q14807-2]
RefSeqiNP_001243198.1. NM_001256269.1. [Q14807-2]
NP_001243199.1. NM_001256270.1. [Q14807-2]
NP_015556.1. NM_007317.2. [Q14807-1]
UniGeneiHs.612151.

Genome annotation databases

EnsembliENST00000160827; ENSP00000160827; ENSG00000079616. [Q14807-1]
ENST00000400751; ENSP00000383562; ENSG00000079616. [Q14807-2]
ENST00000561482; ENSP00000454957; ENSG00000079616. [Q14807-2]
GeneIDi3835.
KEGGihsa:3835.
UCSCiuc002dts.5. human. [Q14807-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017430 mRNA. Translation: BAA33019.2.
AB017335 Genomic DNA. Translation: BAA33063.1.
BT007259 mRNA. Translation: AAP35923.1.
AK294380 mRNA. Translation: BAH11751.1.
AK312234 mRNA. Translation: BAG35167.1.
AK316389 mRNA. Translation: BAH14760.1.
AK223431 mRNA. Translation: BAD97151.1.
AC002301 Genomic DNA. Translation: AAC08709.1. Sequence problems.
AC009133 Genomic DNA. No translation available.
CH471238 Genomic DNA. Translation: EAW80005.1.
CH471238 Genomic DNA. Translation: EAW80007.1. Sequence problems.
BC004352 mRNA. Translation: AAH04352.1.
BC028155 mRNA. Translation: AAH28155.1.
CCDSiCCDS10653.1. [Q14807-1]
CCDS58444.1. [Q14807-2]
RefSeqiNP_001243198.1. NM_001256269.1. [Q14807-2]
NP_001243199.1. NM_001256270.1. [Q14807-2]
NP_015556.1. NM_007317.2. [Q14807-1]
UniGeneiHs.612151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EDUNMR-A570-660[»]
3BFNX-ray2.30A40-400[»]
ProteinModelPortaliQ14807.
SMRiQ14807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110033. 59 interactors.
IntActiQ14807. 46 interactors.
MINTiMINT-156095.
STRINGi9606.ENSP00000160827.

Chemistry databases

ChEMBLiCHEMBL5470.

PTM databases

iPTMnetiQ14807.
PhosphoSitePlusiQ14807.

Polymorphism and mutation databases

BioMutaiKIF22.
DMDMi19863381.

Proteomic databases

EPDiQ14807.
MaxQBiQ14807.
PaxDbiQ14807.
PeptideAtlasiQ14807.
PRIDEiQ14807.

Protocols and materials databases

DNASUi3835.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000160827; ENSP00000160827; ENSG00000079616. [Q14807-1]
ENST00000400751; ENSP00000383562; ENSG00000079616. [Q14807-2]
ENST00000561482; ENSP00000454957; ENSG00000079616. [Q14807-2]
GeneIDi3835.
KEGGihsa:3835.
UCSCiuc002dts.5. human. [Q14807-1]

Organism-specific databases

CTDi3835.
DisGeNETi3835.
GeneCardsiKIF22.
HGNCiHGNC:6391. KIF22.
HPAiHPA041076.
MalaCardsiKIF22.
MIMi603213. gene.
603546. phenotype.
neXtProtiNX_Q14807.
OpenTargetsiENSG00000079616.
Orphaneti93360. Spondyloepimetaphyseal dysplasia with multiple dislocations.
PharmGKBiPA30180.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0242. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOGENOMiHOG000007569.
HOVERGENiHBG052252.
InParanoidiQ14807.
KOiK10403.
OMAiAGQRCGP.
OrthoDBiEOG091G0FFB.
PhylomeDBiQ14807.
TreeFamiTF105233.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000079616-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-983189. Kinesins.
SignaLinkiQ14807.
SIGNORiQ14807.

Miscellaneous databases

EvolutionaryTraceiQ14807.
GeneWikiiKIF22.
GenomeRNAii3835.
PROiQ14807.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000079616.
CleanExiHS_KIF22.
ExpressionAtlasiQ14807. baseline and differential.
GenevisibleiQ14807. HS.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR010994. RuvA_2-like.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00278. HhH1. 2 hits.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF22_HUMAN
AccessioniPrimary (citable) accession number: Q14807
Secondary accession number(s): B2R5M0
, B7Z265, O60845, O94814, Q53F58, Q9BT46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 26, 2001
Last modified: November 30, 2016
This is version 177 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.