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Protein

N-alpha-acetyltransferase 30

Gene

NAA30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate.1 Publication

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-isoleucyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-isoleucyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-tryptophyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-tryptophyl-[protein] + CoA.1 Publication
Acetyl-CoA + an N-terminal-L-methionyl-L-tyrosyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA.1 Publication

GO - Molecular functioni

  • peptide alpha-N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

  • N-terminal peptidyl-methionine acetylation Source: WormBase

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 30 (EC:2.3.1.2561 Publication)
Alternative name(s):
N-acetyltransferase 12
N-acetyltransferase MAK3 homolog
NatC catalytic subunit
Gene namesi
Name:NAA30
Synonyms:C14orf35, MAK3, NAT12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000139977.13.
HGNCiHGNC:19844. NAA30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi122830.
OpenTargetsiENSG00000139977.
PharmGKBiPA165479187.

Polymorphism and mutation databases

BioMutaiNAA30.
DMDMi121948171.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200321 – 362N-alpha-acetyltransferase 30Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1
Modified residuei117PhosphothreonineCombined sources1
Modified residuei152PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei199PhosphoserineCombined sources1
Modified residuei233N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ147X3.
MaxQBiQ147X3.
PaxDbiQ147X3.
PeptideAtlasiQ147X3.
PRIDEiQ147X3.

PTM databases

iPTMnetiQ147X3.
PhosphoSitePlusiQ147X3.

Expressioni

Gene expression databases

BgeeiENSG00000139977.
CleanExiHS_NAT12.
ExpressionAtlasiQ147X3. baseline and differential.
GenevisibleiQ147X3. HS.

Organism-specific databases

HPAiHPA057824.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of NAA35, NAA38 and NAA30.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NAA35Q5VZE52EBI-9106461,EBI-9106478

Protein-protein interaction databases

BioGridi125797. 19 interactors.
IntActiQ147X3. 3 interactors.
MINTiMINT-5004590.
STRINGi9606.ENSP00000452521.

Structurei

3D structure databases

ProteinModelPortaliQ147X3.
SMRiQ147X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 362N-acetyltransferasePROSITE-ProRule annotationAdd BLAST149

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 30Pro-richAdd BLAST26

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3139. Eukaryota.
COG0456. LUCA.
GeneTreeiENSGT00390000005665.
HOGENOMiHOG000196601.
HOVERGENiHBG082671.
InParanoidiQ147X3.
KOiK00670.
OMAiCLRCPQP.
OrthoDBiEOG091G0BAG.
PhylomeDBiQ147X3.
TreeFamiTF105925.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q147X3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVPPGPSS LLPPPAPPAP AAVEPRCPFP AGAALACCSE DEEDDEEHEG
60 70 80 90 100
GGSRSPAGGE SATVAAKGHP CLRCPQPPQE QQQLNGLISP ELRHLRAAAS
110 120 130 140 150
LKSKVLSVAE VAATTATPDG GPRATATKGA GVHSGERPPH SLSSNARTAV
160 170 180 190 200
PSPVEAAAAS DPAAARNGLA EGTEQEEEEE DEQVRLLSSS LTADCSLRSP
210 220 230 240 250
SGREVEPGED RTIRYVRYES ELQMPDIMRL ITKDLSEPYS IYTYRYFIHN
260 270 280 290 300
WPQLCFLAMV GEECVGAIVC KLDMHKKMFR RGYIAMLAVD SKYRRNGIGT
310 320 330 340 350
NLVKKAIYAM VEGDCDEVVL ETEITNKSAL KLYENLGFVR DKRLFRYYLN
360
GVDALRLKLW LR
Length:362
Mass (Da):39,320
Last modified:August 22, 2006 - v1
Checksum:i6C9D69C980C090C0
GO
Isoform 2 (identifier: Q147X3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-79: Missing.

Note: No experimental confirmation available.
Show »
Length:325
Mass (Da):35,580
Checksum:i9A1E7AF84F24143D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03158143 – 79Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH471061 Genomic DNA. Translation: EAW80705.1.
BC118589 mRNA. Translation: AAI18590.1.
BC122557 mRNA. Translation: AAI22558.1.
CCDSiCCDS32088.1. [Q147X3-1]
RefSeqiNP_001011713.2. NM_001011713.2. [Q147X3-1]
UniGeneiHs.165465.

Genome annotation databases

EnsembliENST00000556492; ENSP00000452521; ENSG00000139977. [Q147X3-1]
GeneIDi122830.
KEGGihsa:122830.
UCSCiuc001xcx.5. human. [Q147X3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNAA30_HUMAN
AccessioniPrimary (citable) accession number: Q147X3
Secondary accession number(s): Q0IIN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 22, 2006
Last modified: September 27, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families