Q14773 (ICAM4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intercellular adhesion molecule 4
Short name=ICAM-4

Alternative name(s):
Landsteiner-Wiener blood group glycoprotein
Short name=LW blood group protein
CD_antigen=CD242

Gene names

Name:	ICAM4
Synonyms:	LW

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. Ref.9
Subcellular location	Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted.
Tissue specificity	Erythrocytes.
Post-translational modification	N- and O-glycosylated.
Polymorphism	Responsible for the Landsteiner-Wiener blood group system [MIM:111250]. The molecular basis of the LW(A)=LW5/LW(B)=LW7 blood group antigens is a single variation in position 100; Gln-100 corresponds to LW(A) and Arg-100 to LW(B).
Sequence similarities	Belongs to the immunoglobulin superfamily. ICAM family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Blood group antigen
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell adhesion Inferred from electronic annotation. Source: InterPro regulation of immune response Traceable author statement. Source: Reactome
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement PubMed 6431896. Source: ProtInc plasma membrane Traceable author statement. Source: Reactome
Molecular_function	integrin binding Traceable author statement PubMed 8566017. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q14773-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q14773-2)

The sequence of this isoform differs from the canonical sequence as follows:
     233-271: AWSPAPTALASGSIAALVGILLTVGAAYLCKCLAMKSQA → GEAPL

Isoform 3 (identifier: Q14773-3)

The sequence of this isoform differs from the canonical sequence as follows:
     132-271: KPPHSVILEP...CKCLAMKSQA → SVPGGLLGGD...QSGEMAIHGG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 271	249	Intercellular adhesion molecule 4		PRO_0000014796
Regions
	Topological domain	23 – 240	218	Extracellular Potential
	Transmembrane	241 – 261	21	Helical; Potential
	Topological domain	262 – 271	10	Cytoplasmic Potential
	Domain	62 – 124	63	Ig-like C2-type 1
	Domain	146 – 217	72	Ig-like C2-type 2
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Disulfide bond	69 ↔ 117		By similarity
	Disulfide bond	153 ↔ 210		By similarity
Natural variations
	Alternative sequence	132 – 271	140	KPPHS…MKSQA → SVPGGLLGGDPEAWKPGHLF RKPGALHRPGSGQRDLDRVC CWTPRLLAARDLPRAPQSRR PGGPQQLGTHYTDARLEPRA HSFGLRFHRCPCRDPPHCGR CVPMQVPSYEVPGVKGDVLC RLSEKKRNMKQSGEMAIHGG in isoform 3.		VSP_043229
	Alternative sequence	233 – 271	39	AWSPA…MKSQA → GEAPL in isoform Short.		VSP_002519
	Natural variant	100	1	Q → R in LW(B). Ref.8		VAR_003912
	Natural variant	208	1	V → L. Ref.4		VAR_038721
Experimental info
	Sequence conflict	14 – 29	16	AAAYP…GRRTK → RPPTRELGARWDAGL in AAA59538. Ref.1
	Sequence conflict	14 – 29	16	AAAYP…GRRTK → RPPTRELGARWDAGL in AAA59537. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2F6BC8BABD79E615	FASTA	271	29,265
	10 20 30 40 50 60 MGSLFPLSLL FFLAAAYPGV GSALGRRTKR AQSPKGSPLA PSGTSVPFWV RMSPEFVAVQ 70 80 90 100 110 120 PGKSVQLNCS NSCPQPQNSS LRTPLRQGKT LRGPGWVSYQ LLDVRAWSSL AHCLVTCAGK 130 140 150 160 170 180 TRWATSRITA YKPPHSVILE PPVLKGRKYT LRCHVTQVFP VGYLVVTLRH GSRVIYSESL 190 200 210 220 230 240 ERFTGLDLAN VTLTYEFAAG PRDFWQPVIC HARLNLDGLV VRNSSAPITL MLAWSPAPTA 250 260 270 LASGSIAALV GILLTVGAAY LCKCLAMKSQ A
	Isoform Short [UniParc]. Checksum: F74416D3FB52ADB2	FASTA	237	25,931
	10 20 30 40 50 60 MGSLFPLSLL FFLAAAYPGV GSALGRRTKR AQSPKGSPLA PSGTSVPFWV RMSPEFVAVQ 70 80 90 100 110 120 PGKSVQLNCS NSCPQPQNSS LRTPLRQGKT LRGPGWVSYQ LLDVRAWSSL AHCLVTCAGK 130 140 150 160 170 180 TRWATSRITA YKPPHSVILE PPVLKGRKYT LRCHVTQVFP VGYLVVTLRH GSRVIYSESL 190 200 210 220 230 ERFTGLDLAN VTLTYEFAAG PRDFWQPVIC HARLNLDGLV VRNSSAPITL MLGEAPL
	Isoform 3 [UniParc]. Checksum: 5C19BC5545F0D26E	FASTA	271	29,536
	10 20 30 40 50 60 MGSLFPLSLL FFLAAAYPGV GSALGRRTKR AQSPKGSPLA PSGTSVPFWV RMSPEFVAVQ 70 80 90 100 110 120 PGKSVQLNCS NSCPQPQNSS LRTPLRQGKT LRGPGWVSYQ LLDVRAWSSL AHCLVTCAGK 130 140 150 160 170 180 TRWATSRITA YSVPGGLLGG DPEAWKPGHL FRKPGALHRP GSGQRDLDRV CCWTPRLLAA 190 200 210 220 230 240 RDLPRAPQSR RPGGPQQLGT HYTDARLEPR AHSFGLRFHR CPCRDPPHCG RCVPMQVPSY 250 260 270 EVPGVKGDVL CRLSEKKRNM KQSGEMAIHG G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The LW blood group glycoprotein is homologous to intercellular adhesion molecules." Bailly P., Hermand P., Callebaut I., Sonneborn H.H., Khamlichi S., Mornon J.-P., Cartron J.-P. Proc. Natl. Acad. Sci. U.S.A. 91:5306-5310(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]	"Characterization of the gene encoding the human LW blood group protein in LW+ and LW- phenotypes." Hermand P., le Pennec P.Y., Rouger P., Cartron J.-P., Bailly P. Blood 87:2962-2967(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG).
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	SeattleSNPs variation discovery resource Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-208.
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND 3). Tissue: Lung and Placenta.
[8]	"Molecular basis and expression of the LWa/LWb blood group polymorphism." Hermand P., Gane P., Mattei M.-G., Sistonen P., Cartron J.-P., Bailly P. Blood 86:1590-1594(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-130, VARIANT BLOOD GROUP LW(B) ARG-100.
[9]	"Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms." Spring F.A., Parsons S.F., Ortlepp S., Olsson M.L., Sessions R., Brady R.L., Anstee D.J. Blood 98:458-466(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

dbRBC/BGMUT

Blood group antigen gene mutation database

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27671 mRNA. Translation: AAA59538.1. L27670 mRNA. Translation: AAA59537.1. X93093 Genomic DNA. Translation: CAA63646.1. BT009816 mRNA. Translation: AAP88818.1. DQ011692 Genomic DNA. Translation: AAY16986.1. AC011511 Genomic DNA. No translation available. CH471106 Genomic DNA. Translation: EAW84089.1. BC000046 mRNA. Translation: AAH00046.1. BC029364 mRNA. Translation: AAH29364.1. S78852 mRNA. Translation: AAB35046.1.
IPI	IPI00000118. IPI00386904. IPI00396335.
PIR	I52612. I59300. I80159.
RefSeq	NP_001034221.1. NM_001039132.2. NP_001535.1. NM_001544.4. NP_071772.1. NM_022377.3.
UniGene	Hs.706750.
3D structure databases
ProteinModelPortal	Q14773.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14773. 1 interaction.
STRING	9606.ENSP00000342114.
Polymorphism databases
DMDM	2497309.
Proteomic databases
PaxDb	Q14773.
PRIDE	Q14773.
Protocols and materials databases
DNASU	3386.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000340992; ENSP00000342114; ENSG00000105371. ENST00000380770; ENSP00000370147; ENSG00000105371. ENST00000393717; ENSP00000377320; ENSG00000105371.
GeneID	3386.
KEGG	hsa:3386.
UCSC	uc002mns.2. human. uc002mnt.2. human.
Organism-specific databases
CTD	3386.
GeneCards	GC19P010397.
HGNC	HGNC:5347. ICAM4.
MIM	111250. phenotype. 614088. gene.
neXtProt	NX_Q14773.
PharmGKB	PA29595.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47696.
HOGENOM	HOG000112993.
HOVERGEN	HBG052076.
KO	K06581.
OMA	VIYSESL.
PhylomeDB	Q14773.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_6900. Immune System.
Gene expression databases
Bgee	Q14773.
CleanEx	HS_ICAM4.
Genevestigator	Q14773.
GermOnline	ENSG00000105371. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 2 hits.
InterPro	IPR013768. ICAM_N. IPR003987. ICAM_VCAM_N. IPR013783. Ig-like_fold. [Graphical view]
Pfam	PF03921. ICAM_N. 1 hit. [Graphical view]
PRINTS	PR01472. ICAMVCAM1.
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3386.
NextBio	13396.
SOURCE	Search...

Entry information

Entry name

ICAM4_HUMAN

Accession

Primary (citable) accession number: Q14773
Secondary accession number(s): A0M8X2 , Q14771, Q14772, Q16375, Q9BWR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.