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Reviewed, UniProtKB/Swiss-Prot Q14738 (2A5D_HUMAN)

Last modified February 9, 2010. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Alternative name(s):
    PP2A B subunit isoform B'-delta
    PP2A B subunit isoform B56-delta
    PP2A B subunit isoform PR61-delta
    PP2A B subunit isoform R5-delta
Gene names
Name: PPP2R5D
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. Ref.9

Subcellular location

Cytoplasm. Nucleus. Note: Nuclear in interphase, nuclear during mitosis.

Tissue specificity

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

Induction

By retinoic acid; in neuroblastoma cell lines.

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.11 Ref.12 Ref.13

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit B56 family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC25CP303071EBI-396563,EBI-974439
PPFIA1Q131361EBI-396563,EBI-745426
PPP2R1BP301541EBI-396563,EBI-357094

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Delta-1 (identifier: Q14738-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Delta-2 (identifier: Q14738-2)

The sequence of this isoform differs from the canonical sequence as follows:
     85-116: Missing.
Isoform Delta-3 (identifier: Q14738-3)

The sequence of this isoform differs from the canonical sequence as follows:
     11-116: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
PRO_0000071452

Regions

Repeat37 – 3821
Repeat39 – 4022
Repeat41 – 4223
Repeat43 – 4424
Repeat45 – 4625
Repeat47 – 4826; approximate
Repeat49 – 5027; approximate
Repeat51 – 5228
Region37 – 52168 X 2 AA approximate tandem repeats of Q-P
Motif523 – 5308SH3-binding; class I Potential
Motif548 – 56518Nuclear localization signal Potential

Amino acid modifications

Modified residue5531Phosphothreonine Ref.12
Modified residue5731Phosphoserine Ref.13
Modified residue5981Phosphoserine Ref.10 Ref.11

Natural variations

Alternative sequence11 – 116106Missing in isoform Delta-3.
VSP_005110
Alternative sequence85 – 11632Missing in isoform Delta-2.
VSP_005111

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Sequences

Sequence LengthMass (Da)Tools
Isoform Delta-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F15F71AF4E565387

FASTA60269,992
        10         20         30         40         50         60 
MPYKLKKEKE PPKVAKCTAK PSSSGKDGGG ENTEEAQPQP QPQPQPQAQS QPPSSNKRPS 

        70         80         90        100        110        120 
NSTPPPTQLS KIKYSGGPQI VKKERRQSSS RFNLSKNREL QKLPALKDSP TQEREELFIQ 

       130        140        150        160        170        180 
KLRQCCVLFD FVSDPLSDLK FKEVKRAGLN EMVEYITHSR DVVTEAIYPE AVTMFSVNLF 

       190        200        210        220        230        240 
RTLPPSSNPT GAEFDPEEDE PTLEAAWPHL QLVYEFFLRF LESPDFQPNI AKKYIDQKFV 

       250        260        270        280        290        300 
LALLDLFDSE DPRERDFLKT ILHRIYGKFL GLRAYIRRQI NHIFYRFIYE TEHHNGIAEL 

       310        320        330        340        350        360 
LEILGSIING FALPLKEEHK MFLIRVLLPL HKVKSLSVYH PQLAYCVVQF LEKESSLTEP 

       370        380        390        400        410        420 
VIVGLLKFWP KTHSPKEVMF LNELEEILDV IEPSEFSKVM EPLFRQLAKC VSSPHFQVAE 

       430        440        450        460        470        480 
RALYYWNNEY IMSLISDNAA RVLPIMFPAL YRNSKSHWNK TIHGLIYNAL KLFMEMNQKL 

       490        500        510        520        530        540 
FDDCTQQYKA EKQKGRFRMK EREEMWQKIE ELARLNPQYP MFRAPPPLPP VYSMETETPT 

       550        560        570        580        590        600 
AEDIQLLKRT VETEAVQMLK DIKKEKVLLR RKSELPQDVY TIKALEAHKR AEEFLTASQE 


AL

« Hide

Isoform Delta-2.

Checksum: 978213A7CD1B21A5
Show »

FASTA57066,181
Isoform Delta-3.

Checksum: A57661F8B20731CB
Show »

FASTA49658,453

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References

« Hide 'large scale' references
[1]"The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm."
McCright B., Rivers A.M., Audlin S., Virshup D.M.
J. Biol. Chem. 271:22081-22089(1996) [PubMed: 8703017] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA-1).
Tissue: Fetal brain.
[2]"Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B'' or delta) of human protein phosphatase 2A."
Tanabe O., Gomez G.A., Nishito Y., Usui H., Takeda M.
FEBS Lett. 408:52-56(1997) [PubMed: 9180267] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS DELTA-1 AND DELTA-3).
Tissue: Brain cortex.
[3]"Molecular cloning of a 74-kDa regulatory subunit (B'' or delta) of human protein phosphatase 2A."
Tanabe O., Nagase T., Murakami T., Nozaki H., Usui H., Nishito Y., Hayashi H., Kagamiyama H., Takeda M.
FEBS Lett. 379:107-111(1996) [PubMed: 8566219] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA-2), PROTEIN SEQUENCE OF 501-508; 550-559; 573-580 AND 584-601 (DELTA-1).
Tissue: Bone marrow and Brain cortex.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM DELTA-2).
Tissue: Heart.
[5]"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. expand/collapse author list , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
Nat. Methods 5:1011-1017(2008) [PubMed: 19054851] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM DELTA-1).
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed: 14574404] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS DELTA-1 AND DELTA-2).
Tissue: Colon, Eye and Kidney.
[9]"Shugoshin collaborates with protein phosphatase 2A to protect cohesin."
Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y.
Nature 441:46-52(2006) [PubMed: 16541025] [Abstract]
Cited for: INTERACTION WITH SGOL1.
[10]"Phosphoproteome analysis of the human mitotic spindle."
Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.
Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-598, MASS SPECTROMETRY.
Tissue: Epithelium.
[11]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-598, MASS SPECTROMETRY.
[12]"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.
Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-553, MASS SPECTROMETRY.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-573, MASS SPECTROMETRY.
[14]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L76702 mRNA. Translation: AAB69751.1.
AB000634 mRNA. Translation: BAA20381.1.
AB000635 mRNA. Translation: BAA20382.1.
D78360 mRNA. Translation: BAA11372.1.
AK290604 mRNA. Translation: BAF83293.1.
AB451342 mRNA. Translation: BAG70156.1.
AB451357 mRNA. Translation: BAG70171.1.
AL136304 Genomic DNA. Translation: CAI19791.1.
CH471081 Genomic DNA. Translation: EAX04133.1.
BC010692 mRNA. Translation: AAH10692.1.
BC001095 mRNA. Translation: AAH01095.1.
BC001175 mRNA. Translation: AAH01175.1.
IPIIPI00000030.
IPI00219543.
IPI00219544.
PIRS68686.
RefSeqNP_006236.1.
NP_851307.1.
NP_851308.1.
UniGeneHs.533308

3D structure databases

SMRQ14738. Positions 114-488.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29961N.
IntActQ14738. 15 interactions.
STRINGQ14738.

PTM databases

PhosphoSiteQ14738.

Proteomic databases

PRIDEQ14738.

Genome annotation databases

EnsemblENST00000394110; ENSP00000377669; ENSG00000112640; Homo sapiens. [Genome view]
ENST00000485511; ENSP00000417963; ENSG00000112640; Homo sapiens. [Genome view]
GeneID5528.
KEGGhsa:5528.
UCSCuc003oth.1. human.
uc010jyd.1. human.

Organism-specific databases

CTD5528.
GeneCardsGC06P043060.
H-InvDBHIX0005889.
HGNCHGNC:9312. PPP2R5D.
MIM601646. gene.
PharmGKBPA33676.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG12764.
HOGENOMHBG602178.
HOVERGENQ14738.
InParanoidQ14738.
OMAAVIMFSV.
OrthoDBEOG970X2R.
PhylomeDBQ14738.

Enzyme and pathway databases

Pathway_Interaction_DBalphasynuclein_pathway. Alpha-synuclein signaling.
aurora_a_pathway. Aurora A signaling.
aurora_b_pathway. Aurora B signaling.
wnt_canonical_pathway. Canonical Wnt signaling pathway.
il2_1pathway. IL2-mediated signaling events.
lis1pathway. Lissencephaly gene (LIS1) in neuronal migration and development.
mtor_4pathway. mTOR signaling pathway.
ps1pathway. Presenilin action in Notch and Wnt signaling.
ReactomeREACT_11045. Signaling by Wnt.
REACT_11061. Signalling by NGF.
REACT_1505. Integration of energy metabolism.
REACT_152. Cell Cycle, Mitotic.
REACT_15295. Opioid Signalling.
REACT_6900. Signaling in Immune system.

Gene expression databases

ArrayExpressQ14738.
BgeeQ14738.
CleanExHS_PPP2R5D.
GenevestigatorQ14738.
GermOnlineENSG00000112640. Homo sapiens.

Family and domain databases

InterProIPR002554. PP2A_B56.
[Graphical view]
PANTHERPTHR10257. B56. 1 hit.
PfamPF01603. B56. 1 hit.
[Graphical view]
PIRSFPIRSF028043. PP2A_B56. 1 hit.
ProtoNetSearch...

Other Resources

NextBio21410.
SOURCESearch...

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Entry information

Entry name2A5D_HUMAN
AccessionPrimary (citable) accession number: Q14738
Secondary accession number(s): A8K3I9 expand/collapse secondary AC list , B5BUA6, O00494, O00696, Q15171, Q5TC39
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: February 9, 2010
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents