Q14721 (KCNB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily B member 1

Alternative name(s):
Delayed rectifier potassium channel 1
Short name=DRK1
Short name=h-DRK1
Voltage-gated potassium channel subunit Kv2.1

Gene names

Name:

KCNB1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
Subunit structure	Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Post-translational modification	Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively By similarity.
Sequence similarities	Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily. [View classification]
Sequence caution	The sequence AAA36156.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	energy reserve metabolic process Traceable author statement. Source: Reactome protein homooligomerization Inferred from electronic annotation. Source: InterPro regulation of insulin secretion Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	dendrite Inferred from electronic annotation. Source: Compara dendrite membrane Inferred from electronic annotation. Source: Compara neuronal cell body membrane Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement. Source: Reactome postsynaptic membrane Inferred from electronic annotation. Source: Compara voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome outward rectifier potassium channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	Potassium voltage-gated channel subfamily B member 1		PRO_0000054042
Regions
	Topological domain	1 – 186	186	Cytoplasmic Potential
	Transmembrane	187 – 208	22	Helical; Name=Segment S1; Potential
	Transmembrane	229 – 250	22	Helical; Name=Segment S2; Potential
	Topological domain	251 – 260	10	Cytoplasmic Potential
	Transmembrane	261 – 282	22	Helical; Name=Segment S3; Potential
	Transmembrane	295 – 316	22	Helical; Voltage-sensor; Name=Segment S4; Potential
	Topological domain	317 – 330	14	Cytoplasmic Potential
	Transmembrane	331 – 352	22	Helical; Name=Segment S5; Potential
	Intramembrane	365 – 385	21	Pore-forming; Name=Segment H5; Potential
	Transmembrane	393 – 414	22	Helical; Name=Segment S6; Potential
	Topological domain	415 – 858	444	Cytoplasmic Potential
	Motif	377 – 382	6	Selectivity filter By similarity
	Compositional bias	517 – 520	4	Poly-Ser
	Compositional bias	701 – 706	6	Poly-Ala
Amino acid modifications
	Modified residue	12	1	Phosphoserine By similarity
	Modified residue	15	1	Phosphoserine By similarity
	Modified residue	128	1	Phosphotyrosine; by Src By similarity
	Modified residue	457	1	Phosphoserine By similarity
	Modified residue	484	1	Phosphoserine By similarity
	Modified residue	496	1	Phosphoserine By similarity
	Modified residue	503	1	Phosphoserine By similarity
	Modified residue	520	1	Phosphoserine By similarity
	Modified residue	541	1	Phosphoserine By similarity
	Modified residue	567	1	Phosphoserine By similarity
	Modified residue	590	1	Phosphoserine By similarity
	Modified residue	607	1	Phosphoserine By similarity
	Modified residue	656	1	Phosphoserine By similarity
	Modified residue	720	1	Phosphoserine By similarity
	Modified residue	772	1	Phosphoserine By similarity
	Modified residue	800	1	Phosphoserine By similarity
	Modified residue	805	1	Phosphoserine By similarity
Natural variations
	Natural variant	616	1	T → N. Corresponds to variant rs2229006 [ dbSNP | Ensembl ].		VAR_062182
	Natural variant	616	1	T → S. Corresponds to variant rs2229006 [ dbSNP | Ensembl ].		VAR_062183
	Natural variant	825	1	P → S. Corresponds to variant rs34467662 [ dbSNP | Ensembl ].		VAR_034049
	Natural variant	857	1	S → N. Corresponds to variant rs34280195 [ dbSNP | Ensembl ].		VAR_062184

Sequences

	Sequence		Length	Mass (Da)
	Q14721 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: C4B426174ED0DEE4	FASTA	858	95,878
	10 20 30 40 50 60 MPAGMTKHGS RSTSSLPPEP MEIVRSKACS RRVRLNVGGL AHEVLWRTLD RLPRTRLGKL 70 80 90 100 110 120 RDCNTHDSLL EVCDDYSLDD NEYFFDRHPG AFTSILNFYR TGRLHMMEEM CALSFSQELD 130 140 150 160 170 180 YWGIDEIYLE SCCQARYHQK KEQMNEELKR EAETLREREG EEFDNTCCAE KRKKLWDLLE 190 200 210 220 230 240 KPNSSVAAKI LAIISIMFIV LSTIALSLNT LPELQSLDEF GQSTDNPQLA HVEAVCIAWF 250 260 270 280 290 300 TMEYLLRFLS SPKKWKFFKG PLNAIDLLAI LPYYVTIFLT ESNKSVLQFQ NVRRVVQIFR 310 320 330 340 350 360 IMRILRILKL ARHSTGLQSL GFTLRRSYNE LGLLILFLAM GIMIFSSLVF FAEKDEDDTK 370 380 390 400 410 420 FKSIPASFWW ATITMTTVGY GDIYPKTLLG KIVGGLCCIA GVLVIALPIP IIVNNFSEFY 430 440 450 460 470 480 KEQKRQEKAI KRREALERAK RNGSIVSMNM KDAFARSIEM MDIVVEKNGE NMGKKDKVQD 490 500 510 520 530 540 NHLSPNKWKW TKRTLSETSS SKSFETKEQG SPEKARSSSS PQHLNVQQLE DMYNKMAKTQ 550 560 570 580 590 600 SQPILNTKES AAQSKPKEEL EMESIPSPVA PLPTRTEGVI DMRSMSSIDS FISCATDFPE 610 620 630 640 650 660 ATRFSHSPLT SLPSKTGGST APEVGWRGAL GASGGRFVEA NPSPDASQHS SFFIESPKSS 670 680 690 700 710 720 MKTNNPLKLR ALKVNFMEGD PSPLLPVLGM YHDPLRNRGS AAAAVAGLEC ATLLDKAVLS 730 740 750 760 770 780 PESSIYTTAS AKTPPRSPEK HTAIAFNFEA GVHQYIDADT DDEGQLLYSV DSSPPKSLPG 790 800 810 820 830 840 STSPKFSTGT RSEKNHFESS PLPTSPKFLR QNCIYSTEAL TGKGPSGQEK CKLENHISPD 850 VRVLPGGGAH GSTRDQSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a human delayed rectifier potassium channel gene." Albrecht B., Lorra C., Stocker K., Pongs O. Recept. Channels 1:99-110(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Heterologous expression of the human potassium channel Kv2.1 in clonal mammalian cells by direct cytoplasmic microinjection of cRNA." Ikeda S.R., Soler F., Zuhlke R.D., Joho R.H., Lewis D.L. Pflugers Arch. 422:201-203(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain cortex.
[3]	"Identification of potassium channels in human lens epithelium." Rae J.L., Shepard A.R. (In) Civan M.M. (eds.); The eye's aqueous humor-from secretion to glaucoma, pp.69-104, Academic Press, San Diego (1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens epithelium.
[4]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68302 Genomic DNA. Translation: CAA48374.1. L02840 mRNA. Translation: AAA36156.1. Different initiation. AF026005 mRNA. Translation: AAB88808.1. AL035685 Genomic DNA. Translation: CAB89417.1.
IPI	IPI00033019.
PIR	S31761.
RefSeq	NP_004966.1. NM_004975.2.
UniGene	Hs.84244.
3D structure databases
ProteinModelPortal	Q14721.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14721. 1 interaction.
STRING	9606.ENSP00000360806.
Protein family/group databases
TCDB	1.A.1.2.11. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	Q14721.
Polymorphism databases
DMDM	24418854.
2D gel databases
OGP	Q14721.
Proteomic databases
PaxDb	Q14721.
PRIDE	Q14721.
Protocols and materials databases
DNASU	3745.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371741; ENSP00000360806; ENSG00000158445.
GeneID	3745.
KEGG	hsa:3745.
UCSC	uc002xur.1. human.
Organism-specific databases
CTD	3745.
GeneCards	GC20M047984.
HGNC	HGNC:6231. KCNB1.
HPA	CAB001979.
MIM	600397. gene.
neXtProt	NX_Q14721.
PharmGKB	PA209.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1226.
HOGENOM	HOG000113206.
HOVERGEN	HBG052225.
InParanoid	Q14721.
KO	K04885.
OMA	QLEDMYN.
OrthoDB	EOG4X6C7Q.
PhylomeDB	Q14721.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_13685. Neuronal System.
Gene expression databases
Bgee	Q14721.
CleanEx	HS_KCNB1.
Genevestigator	Q14721.
GermOnline	ENSG00000158445. Homo sapiens.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003973. K_chnl_volt-dep_Kv2. IPR004350. K_chnl_volt-dep_Kv2.1. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. PF03521. Kv2channel. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01514. KV21CHANNEL. PR01491. KVCHANNEL. PR01495. SHABCHANNEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	3745.
NextBio	14655.
SOURCE	Search...

Entry information

Entry name

KCNB1_HUMAN

Accession

Primary (citable) accession number: Q14721
Secondary accession number(s): Q14193

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 25, 2002
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.