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Q14721

- KCNB1_HUMAN

UniProt

Q14721 - KCNB1_HUMAN

Protein

Potassium voltage-gated channel subfamily B member 1

Gene

KCNB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (25 Oct 2002)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome
    2. outward rectifier potassium channel activity Source: Ensembl
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. energy reserve metabolic process Source: Reactome
    2. protein homooligomerization Source: InterPro
    3. regulation of insulin secretion Source: Reactome
    4. small molecule metabolic process Source: Reactome
    5. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_75770. Voltage gated Potassium channels.

    Protein family/group databases

    TCDBi1.A.1.2.11. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily B member 1
    Alternative name(s):
    Delayed rectifier potassium channel 1
    Short name:
    DRK1
    Short name:
    h-DRK1
    Voltage-gated potassium channel subunit Kv2.1
    Gene namesi
    Name:KCNB1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:6231. KCNB1.

    Subcellular locationi

    GO - Cellular componenti

    1. dendrite Source: Ensembl
    2. dendrite membrane Source: Ensembl
    3. neuronal cell body membrane Source: Ensembl
    4. plasma membrane Source: Reactome
    5. postsynaptic membrane Source: Ensembl
    6. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA209.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 858858Potassium voltage-gated channel subfamily B member 1PRO_0000054042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151PhosphoserineBy similarity
    Modified residuei128 – 1281Phosphotyrosine; by SrcBy similarity
    Modified residuei457 – 4571PhosphoserineBy similarity
    Modified residuei484 – 4841PhosphoserineBy similarity
    Modified residuei496 – 4961PhosphoserineBy similarity
    Modified residuei503 – 5031PhosphoserineBy similarity
    Modified residuei520 – 5201PhosphoserineBy similarity
    Modified residuei541 – 5411PhosphoserineBy similarity
    Modified residuei567 – 5671PhosphoserineBy similarity
    Modified residuei590 – 5901PhosphoserineBy similarity
    Modified residuei607 – 6071PhosphoserineBy similarity
    Modified residuei656 – 6561PhosphoserineBy similarity
    Modified residuei720 – 7201PhosphoserineBy similarity
    Modified residuei772 – 7721PhosphoserineBy similarity
    Modified residuei800 – 8001PhosphoserineBy similarity
    Modified residuei805 – 8051PhosphoserineBy similarity

    Post-translational modificationi

    Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ14721.
    PRIDEiQ14721.

    2D gel databases

    OGPiQ14721.

    PTM databases

    PhosphoSiteiQ14721.

    Expressioni

    Gene expression databases

    BgeeiQ14721.
    CleanExiHS_KCNB1.
    GenevestigatoriQ14721.

    Organism-specific databases

    HPAiCAB001979.

    Interactioni

    Subunit structurei

    Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2.By similarity

    Protein-protein interaction databases

    BioGridi109947. 16 interactions.
    IntActiQ14721. 1 interaction.
    STRINGi9606.ENSP00000360806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ14721.
    SMRiQ14721. Positions 30-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 186186CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini251 – 26010CytoplasmicSequence Analysis
    Topological domaini317 – 33014CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini415 – 858444CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei365 – 38521Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei187 – 20822Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei229 – 25022Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei261 – 28222Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei295 – 31622Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei331 – 35222Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei393 – 41422Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi377 – 3826Selectivity filterBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi517 – 5204Poly-Ser
    Compositional biasi701 – 7066Poly-Ala

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    HOGENOMiHOG000113206.
    HOVERGENiHBG052225.
    InParanoidiQ14721.
    KOiK04885.
    OMAiCATDFPE.
    OrthoDBiEOG7CRTPP.
    PhylomeDBiQ14721.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003973. K_chnl_volt-dep_Kv2.
    IPR004350. K_chnl_volt-dep_Kv2.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF03521. Kv2channel. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01514. KV21CHANNEL.
    PR01491. KVCHANNEL.
    PR01495. SHABCHANNEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q14721-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAGMTKHGS RSTSSLPPEP MEIVRSKACS RRVRLNVGGL AHEVLWRTLD    50
    RLPRTRLGKL RDCNTHDSLL EVCDDYSLDD NEYFFDRHPG AFTSILNFYR 100
    TGRLHMMEEM CALSFSQELD YWGIDEIYLE SCCQARYHQK KEQMNEELKR 150
    EAETLREREG EEFDNTCCAE KRKKLWDLLE KPNSSVAAKI LAIISIMFIV 200
    LSTIALSLNT LPELQSLDEF GQSTDNPQLA HVEAVCIAWF TMEYLLRFLS 250
    SPKKWKFFKG PLNAIDLLAI LPYYVTIFLT ESNKSVLQFQ NVRRVVQIFR 300
    IMRILRILKL ARHSTGLQSL GFTLRRSYNE LGLLILFLAM GIMIFSSLVF 350
    FAEKDEDDTK FKSIPASFWW ATITMTTVGY GDIYPKTLLG KIVGGLCCIA 400
    GVLVIALPIP IIVNNFSEFY KEQKRQEKAI KRREALERAK RNGSIVSMNM 450
    KDAFARSIEM MDIVVEKNGE NMGKKDKVQD NHLSPNKWKW TKRTLSETSS 500
    SKSFETKEQG SPEKARSSSS PQHLNVQQLE DMYNKMAKTQ SQPILNTKES 550
    AAQSKPKEEL EMESIPSPVA PLPTRTEGVI DMRSMSSIDS FISCATDFPE 600
    ATRFSHSPLT SLPSKTGGST APEVGWRGAL GASGGRFVEA NPSPDASQHS 650
    SFFIESPKSS MKTNNPLKLR ALKVNFMEGD PSPLLPVLGM YHDPLRNRGS 700
    AAAAVAGLEC ATLLDKAVLS PESSIYTTAS AKTPPRSPEK HTAIAFNFEA 750
    GVHQYIDADT DDEGQLLYSV DSSPPKSLPG STSPKFSTGT RSEKNHFESS 800
    PLPTSPKFLR QNCIYSTEAL TGKGPSGQEK CKLENHISPD VRVLPGGGAH 850
    GSTRDQSI 858
    Length:858
    Mass (Da):95,878
    Last modified:October 25, 2002 - v2
    Checksum:iC4B426174ED0DEE4
    GO

    Sequence cautioni

    The sequence AAA36156.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti616 – 6161T → N.
    Corresponds to variant rs2229006 [ dbSNP | Ensembl ].
    VAR_062182
    Natural varianti616 – 6161T → S.
    Corresponds to variant rs2229006 [ dbSNP | Ensembl ].
    VAR_062183
    Natural varianti825 – 8251P → S.
    Corresponds to variant rs34467662 [ dbSNP | Ensembl ].
    VAR_034049
    Natural varianti857 – 8571S → N.
    Corresponds to variant rs34280195 [ dbSNP | Ensembl ].
    VAR_062184

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68302 Genomic DNA. Translation: CAA48374.1.
    L02840 mRNA. Translation: AAA36156.1. Different initiation.
    AF026005 mRNA. Translation: AAB88808.1.
    AL035685 Genomic DNA. Translation: CAB89417.1.
    CCDSiCCDS13418.1.
    PIRiS31761.
    RefSeqiNP_004966.1. NM_004975.2.
    XP_006723847.1. XM_006723784.1.
    UniGeneiHs.84244.

    Genome annotation databases

    EnsembliENST00000371741; ENSP00000360806; ENSG00000158445.
    GeneIDi3745.
    KEGGihsa:3745.
    UCSCiuc002xur.1. human.

    Polymorphism databases

    DMDMi24418854.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68302 Genomic DNA. Translation: CAA48374.1 .
    L02840 mRNA. Translation: AAA36156.1 . Different initiation.
    AF026005 mRNA. Translation: AAB88808.1 .
    AL035685 Genomic DNA. Translation: CAB89417.1 .
    CCDSi CCDS13418.1.
    PIRi S31761.
    RefSeqi NP_004966.1. NM_004975.2.
    XP_006723847.1. XM_006723784.1.
    UniGenei Hs.84244.

    3D structure databases

    ProteinModelPortali Q14721.
    SMRi Q14721. Positions 30-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109947. 16 interactions.
    IntActi Q14721. 1 interaction.
    STRINGi 9606.ENSP00000360806.

    Chemistry

    ChEMBLi CHEMBL2362996.
    GuidetoPHARMACOLOGYi 546.

    Protein family/group databases

    TCDBi 1.A.1.2.11. the voltage-gated ion channel (vic) superfamily.

    PTM databases

    PhosphoSitei Q14721.

    Polymorphism databases

    DMDMi 24418854.

    2D gel databases

    OGPi Q14721.

    Proteomic databases

    PaxDbi Q14721.
    PRIDEi Q14721.

    Protocols and materials databases

    DNASUi 3745.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000371741 ; ENSP00000360806 ; ENSG00000158445 .
    GeneIDi 3745.
    KEGGi hsa:3745.
    UCSCi uc002xur.1. human.

    Organism-specific databases

    CTDi 3745.
    GeneCardsi GC20M047984.
    HGNCi HGNC:6231. KCNB1.
    HPAi CAB001979.
    MIMi 600397. gene.
    neXtProti NX_Q14721.
    PharmGKBi PA209.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1226.
    HOGENOMi HOG000113206.
    HOVERGENi HBG052225.
    InParanoidi Q14721.
    KOi K04885.
    OMAi CATDFPE.
    OrthoDBi EOG7CRTPP.
    PhylomeDBi Q14721.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
    REACT_75770. Voltage gated Potassium channels.

    Miscellaneous databases

    GeneWikii KCNB1.
    GenomeRNAii 3745.
    NextBioi 14655.
    PROi Q14721.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q14721.
    CleanExi HS_KCNB1.
    Genevestigatori Q14721.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003973. K_chnl_volt-dep_Kv2.
    IPR004350. K_chnl_volt-dep_Kv2.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF03521. Kv2channel. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01514. KV21CHANNEL.
    PR01491. KVCHANNEL.
    PR01495. SHABCHANNEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a human delayed rectifier potassium channel gene."
      Albrecht B., Lorra C., Stocker K., Pongs O.
      Recept. Channels 1:99-110(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Heterologous expression of the human potassium channel Kv2.1 in clonal mammalian cells by direct cytoplasmic microinjection of cRNA."
      Ikeda S.R., Soler F., Zuhlke R.D., Joho R.H., Lewis D.L.
      Pflugers Arch. 422:201-203(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain cortex.
    3. "Identification of potassium channels in human lens epithelium."
      Rae J.L., Shepard A.R.
      (In) Civan M.M. (eds.); The eye's aqueous humor-from secretion to glaucoma, pp.69-104, Academic Press, San Diego (1998)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lens epithelium.
    4. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiKCNB1_HUMAN
    AccessioniPrimary (citable) accession number: Q14721
    Secondary accession number(s): Q14193
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: October 25, 2002
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3