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Q146K5

- BIOB_BURXL

UniProt

Q146K5 - BIOB_BURXL

Protein

Biotin synthase

Gene

bioB

Organism
Burkholderia xenovorans (strain LB400)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi84 – 841Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi121 – 1211Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi152 – 1521Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi212 – 2121Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi284 – 2841Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBXEN266265:GJII-201-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Bxeno_A0196
    ORF Names:Bxe_A4266
    OrganismiBurkholderia xenovorans (strain LB400)
    Taxonomic identifieri266265 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
    ProteomesiUP000001817: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Biotin synthasePRO_0000381278Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi266265.Bxe_A4266.

    Structurei

    3D structure databases

    ProteinModelPortaliQ146K5.
    SMRiQ146K5. Positions 30-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q146K5-1 [UniParc]FASTAAdd to Basket

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    MTQLNIAPAS TDTAAASNNN AAAGTKQLAR WRVADIVSLY ELPFNDLMFQ    50
    AQQTHREHFD ANTVQLSTLL SIKTGGCEED CAYCPQSVHH DTGLQADKLM 100
    PVDEVLAAAK VAKENGATRF CMGAAWRNPK DRHLEPIKDM IRGVKAMGLE 150
    TCVTLGMLET HQAQGLREAG LDYYNHNLDT SPEFYGQIIS TRTYQDRLDT 200
    LERVRDAGIN VCCGGIVGLG ESRRERAGLI AQLANMDPYP ESVPINNLVQ 250
    VEGTPLTGTE AIDPFEFVRT IAIARITMPR AMVRLSAGRE QMNEALQAMC 300
    FLAGANSIFY GDQLLTTSNP QAEADRKLLE RLGIRAEAAQ QMPLDQSGCE 350
    HGCDKHAAPN 360
    Length:360
    Mass (Da):39,393
    Last modified:August 22, 2006 - v1
    Checksum:i30FC4AFECEF3C575
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000270 Genomic DNA. Translation: ABE28734.1.
    RefSeqiWP_011486579.1. NC_007951.1.
    YP_556786.1. NC_007951.1.

    Genome annotation databases

    EnsemblBacteriaiABE28734; ABE28734; Bxe_A4266.
    GeneIDi4003941.
    KEGGibxe:Bxe_A4266.
    PATRICi19325731. VBIBurXen52548_0207.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000270 Genomic DNA. Translation: ABE28734.1 .
    RefSeqi WP_011486579.1. NC_007951.1.
    YP_556786.1. NC_007951.1.

    3D structure databases

    ProteinModelPortali Q146K5.
    SMRi Q146K5. Positions 30-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266265.Bxe_A4266.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE28734 ; ABE28734 ; Bxe_A4266 .
    GeneIDi 4003941.
    KEGGi bxe:Bxe_A4266.
    PATRICi 19325731. VBIBurXen52548_0207.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci BXEN266265:GJII-201-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LB400.

    Entry informationi

    Entry nameiBIOB_BURXL
    AccessioniPrimary (citable) accession number: Q146K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3