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Q146K5

- BIOB_BURXL

UniProt

Q146K5 - BIOB_BURXL

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Protein

Biotin synthase

Gene
bioB, Bxeno_A0196, Bxe_A4266
Organism
Burkholderia xenovorans (strain LB400)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi84 – 841Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi121 – 1211Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi212 – 2121Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi284 – 2841Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBXEN266265:GJII-201-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Bxeno_A0196
ORF Names:Bxe_A4266
OrganismiBurkholderia xenovorans (strain LB400)
Taxonomic identifieri266265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001817: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Biotin synthaseUniRule annotationPRO_0000381278Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi266265.Bxe_A4266.

Structurei

3D structure databases

ProteinModelPortaliQ146K5.
SMRiQ146K5. Positions 30-337.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q146K5-1 [UniParc]FASTAAdd to Basket

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MTQLNIAPAS TDTAAASNNN AAAGTKQLAR WRVADIVSLY ELPFNDLMFQ    50
AQQTHREHFD ANTVQLSTLL SIKTGGCEED CAYCPQSVHH DTGLQADKLM 100
PVDEVLAAAK VAKENGATRF CMGAAWRNPK DRHLEPIKDM IRGVKAMGLE 150
TCVTLGMLET HQAQGLREAG LDYYNHNLDT SPEFYGQIIS TRTYQDRLDT 200
LERVRDAGIN VCCGGIVGLG ESRRERAGLI AQLANMDPYP ESVPINNLVQ 250
VEGTPLTGTE AIDPFEFVRT IAIARITMPR AMVRLSAGRE QMNEALQAMC 300
FLAGANSIFY GDQLLTTSNP QAEADRKLLE RLGIRAEAAQ QMPLDQSGCE 350
HGCDKHAAPN 360
Length:360
Mass (Da):39,393
Last modified:August 22, 2006 - v1
Checksum:i30FC4AFECEF3C575
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000270 Genomic DNA. Translation: ABE28734.1.
RefSeqiWP_011486579.1. NC_007951.1.
YP_556786.1. NC_007951.1.

Genome annotation databases

EnsemblBacteriaiABE28734; ABE28734; Bxe_A4266.
GeneIDi4003941.
KEGGibxe:Bxe_A4266.
PATRICi19325731. VBIBurXen52548_0207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000270 Genomic DNA. Translation: ABE28734.1 .
RefSeqi WP_011486579.1. NC_007951.1.
YP_556786.1. NC_007951.1.

3D structure databases

ProteinModelPortali Q146K5.
SMRi Q146K5. Positions 30-337.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266265.Bxe_A4266.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE28734 ; ABE28734 ; Bxe_A4266 .
GeneIDi 4003941.
KEGGi bxe:Bxe_A4266.
PATRICi 19325731. VBIBurXen52548_0207.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci BXEN266265:GJII-201-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LB400.

Entry informationi

Entry nameiBIOB_BURXL
AccessioniPrimary (citable) accession number: Q146K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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