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Q146K5 (BIOB_BURXL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Bxeno_A0196
ORF Names:Bxe_A4266
OrganismBurkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]
Taxonomic identifier266265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Biotin synthase HAMAP-Rule MF_01694
PRO_0000381278

Sites

Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding841Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1211Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1521Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2121Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2841Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q146K5 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 30FC4AFECEF3C575

FASTA36039,393
        10         20         30         40         50         60 
MTQLNIAPAS TDTAAASNNN AAAGTKQLAR WRVADIVSLY ELPFNDLMFQ AQQTHREHFD 

        70         80         90        100        110        120 
ANTVQLSTLL SIKTGGCEED CAYCPQSVHH DTGLQADKLM PVDEVLAAAK VAKENGATRF 

       130        140        150        160        170        180 
CMGAAWRNPK DRHLEPIKDM IRGVKAMGLE TCVTLGMLET HQAQGLREAG LDYYNHNLDT 

       190        200        210        220        230        240 
SPEFYGQIIS TRTYQDRLDT LERVRDAGIN VCCGGIVGLG ESRRERAGLI AQLANMDPYP 

       250        260        270        280        290        300 
ESVPINNLVQ VEGTPLTGTE AIDPFEFVRT IAIARITMPR AMVRLSAGRE QMNEALQAMC 

       310        320        330        340        350        360 
FLAGANSIFY GDQLLTTSNP QAEADRKLLE RLGIRAEAAQ QMPLDQSGCE HGCDKHAAPN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000270 Genomic DNA. Translation: ABE28734.1.
RefSeqYP_556786.1. NC_007951.1.

3D structure databases

ProteinModelPortalQ146K5.
SMRQ146K5. Positions 30-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266265.Bxe_A4266.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE28734; ABE28734; Bxe_A4266.
GeneID4003941.
KEGGbxe:Bxe_A4266.
PATRIC19325731. VBIBurXen52548_0207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2313987.

Enzyme and pathway databases

BioCycBXEN266265:GJII-201-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_BURXL
AccessionPrimary (citable) accession number: Q146K5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways