Reviewed, UniProtKB/Swiss-Prot Q146J2 (ACEK_BURXL)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Bxeno_A0209
ORF Names:	Bxe_A4253

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000259149
Regions
	Nucleotide binding	327 – 333	7	ATP By similarity
Sites
	Active site	383	1	By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q146J2-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 00164CFD1609E5D8	FASTA	612	70,542
	10 20 30 40 50 60 MNHFPKLLSS QIGFDVAQTM LEGFDRHYRI FRDAAIHAKT LFEQADWHGL QKLARDRITS 70 80 90 100 110 120 YDERVEECVE LLEDEYDAEN IDDEVWQQIK LHYIGLLTTH RQPECAETFF NSVCCKILHR 130 140 150 160 170 180 SYFSNDFIFV RPAISTEYIE NDEPAAKPTY RAYYPGKDGL AATLERIVTN FQLEPPFEDL 190 200 210 220 230 240 TRDVGCVMQA IQDAFGVFDE APNFQIHVLS SLFYRNKSAY IIGRIINGDL LMPFAVPLRH 250 260 270 280 290 300 VKPGVLALDT VLLKRDQLLI IFSFSHSYFL VDMEVPSAYV EFLGTIMQGK PKAEIYTSVG 310 320 330 340 350 360 LQKQGKNLFY RDLLHHLSHS SDQFIIAPGI KGLVMLVFTL PSFPYVFKLI KDSFPPPKET 370 380 390 400 410 420 TRAQIKEKYQ LVKRHDRLGR MADTLEYSSV ALPVSRLDEA LVRELEKEVP SLIEYDGDSL 430 440 450 460 470 480 VIRHMYIERR MVPLNLFLQN GNDEDIEHGI KEYGNAIKEL MQANIFPGDM LYKNFGVTRH 490 500 510 520 530 540 GRVVFYDYDE IEYLTDCNVR AVPAPRNEED EMSGEPWYSV GPHDIFPETY GTFLLGDPRV 550 560 570 580 590 600 RRSFMQHHAD FFDPALWQRH KDHLLKGELA DFFPYDGSVR FCMRYPERFA DAAGAASNEQ 610 DAPDAGRSVR AA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000270 Genomic DNA. Translation: ABE28747.1.
RefSeq	YP_556799.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4004013.
GenomeReviews	Gene locus Bxeno_A0209 in contig CP000270_GR.
KEGG	bxe:Bxe_A4253.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q146J2.
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_BURXL

Accession

Primary (citable) accession number: Q146J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)