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Q14691

- PSF1_HUMAN

UniProt

Q14691 - PSF1_HUMAN

Protein

DNA replication complex GINS protein PSF1

Gene

GINS1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.1 Publication

    GO - Biological processi

    1. DNA strand elongation involved in DNA replication Source: Reactome
    2. inner cell mass cell proliferation Source: Ensembl
    3. mitotic cell cycle Source: Reactome

    Keywords - Biological processi

    DNA replication

    Enzyme and pathway databases

    ReactomeiREACT_6776. Unwinding of DNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication complex GINS protein PSF1
    Alternative name(s):
    GINS complex subunit 1
    Gene namesi
    Name:GINS1
    Synonyms:KIAA0186, PSF1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 20

    Organism-specific databases

    HGNCiHGNC:28980. GINS1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA145008291.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196DNA replication complex GINS protein PSF1PRO_0000219035Add
    BLAST

    Proteomic databases

    MaxQBiQ14691.
    PaxDbiQ14691.
    PeptideAtlasiQ14691.
    PRIDEiQ14691.

    PTM databases

    PhosphoSiteiQ14691.

    Expressioni

    Inductioni

    Significantly up-regulated in aggressive melanomas.1 Publication

    Gene expression databases

    ArrayExpressiQ14691.
    BgeeiQ14691.
    CleanExiHS_GINS1.
    GenevestigatoriQ14691.

    Organism-specific databases

    HPAiHPA051185.
    HPA054486.

    Interactioni

    Subunit structurei

    Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS4. GINS complex interacts with DNA primase in vitro.4 Publications

    Protein-protein interaction databases

    BioGridi115174. 5 interactions.
    DIPiDIP-29331N.
    STRINGi9606.ENSP00000262460.

    Structurei

    Secondary structure

    1
    196
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1512
    Beta strandi16 – 194
    Helixi26 – 4924
    Helixi56 – 583
    Helixi59 – 9436
    Helixi100 – 1045
    Helixi108 – 12720
    Beta strandi128 – 1325

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E9XX-ray2.30A/E1-149[»]
    2EHOX-ray3.00B/F/J1-151[»]
    2Q9QX-ray2.36C/G1-196[»]
    ProteinModelPortaliQ14691.
    SMRiQ14691. Positions 1-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ14691.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GINS1/PSF1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5230.
    HOGENOMiHOG000192102.
    HOVERGENiHBG053743.
    InParanoidiQ14691.
    KOiK10732.
    OMAiLQRNKRC.
    OrthoDBiEOG7M6D8F.
    PhylomeDBiQ14691.
    TreeFamiTF312848.

    Family and domain databases

    InterProiIPR005339. GINS_Psf1.
    [Graphical view]
    PANTHERiPTHR12914. PTHR12914. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q14691-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA    50
    KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRAL RWEYGSVLPN 100
    ALRFHMAAEE MEWFNNYKRS LATYMRSLGG DEGLDITQDM KPPKSLYIEV 150
    RCLKDYGEFE VDDGTSVLLK KNSQHFLPRW KCEQLIRQGV LEHILS 196
    Length:196
    Mass (Da):22,988
    Last modified:November 1, 1996 - v1
    Checksum:i2270B5CE6C43BFE5
    GO

    Sequence cautioni

    The sequence BAA11503.2 differs from that shown. Reason: Erroneous initiation.

    Mass spectrometryi

    Molecular mass is 98373±13 Da from positions 1 - 196. Determined by ESI. This is the measured mass for the GINS complex.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti97 – 971V → I.1 Publication
    Corresponds to variant rs6076347 [ dbSNP | Ensembl ].
    VAR_051606

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D80008 mRNA. Translation: BAA11503.2. Different initiation.
    AL353812, AL031672 Genomic DNA. Translation: CAI13764.1.
    AL031672, AL353812 Genomic DNA. Translation: CAI43019.1.
    BC012542 mRNA. Translation: AAH12542.1.
    CCDSiCCDS33451.1.
    RefSeqiNP_066545.3. NM_021067.3.
    UniGeneiHs.658464.

    Genome annotation databases

    EnsembliENST00000262460; ENSP00000262460; ENSG00000101003.
    GeneIDi9837.
    KEGGihsa:9837.
    UCSCiuc002wuv.1. human.

    Polymorphism databases

    DMDMi6226339.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D80008 mRNA. Translation: BAA11503.2 . Different initiation.
    AL353812 , AL031672 Genomic DNA. Translation: CAI13764.1 .
    AL031672 , AL353812 Genomic DNA. Translation: CAI43019.1 .
    BC012542 mRNA. Translation: AAH12542.1 .
    CCDSi CCDS33451.1.
    RefSeqi NP_066545.3. NM_021067.3.
    UniGenei Hs.658464.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E9X X-ray 2.30 A/E 1-149 [» ]
    2EHO X-ray 3.00 B/F/J 1-151 [» ]
    2Q9Q X-ray 2.36 C/G 1-196 [» ]
    ProteinModelPortali Q14691.
    SMRi Q14691. Positions 1-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115174. 5 interactions.
    DIPi DIP-29331N.
    STRINGi 9606.ENSP00000262460.

    PTM databases

    PhosphoSitei Q14691.

    Polymorphism databases

    DMDMi 6226339.

    Proteomic databases

    MaxQBi Q14691.
    PaxDbi Q14691.
    PeptideAtlasi Q14691.
    PRIDEi Q14691.

    Protocols and materials databases

    DNASUi 9837.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262460 ; ENSP00000262460 ; ENSG00000101003 .
    GeneIDi 9837.
    KEGGi hsa:9837.
    UCSCi uc002wuv.1. human.

    Organism-specific databases

    CTDi 9837.
    GeneCardsi GC20P025389.
    HGNCi HGNC:28980. GINS1.
    HPAi HPA051185.
    HPA054486.
    MIMi 610608. gene.
    neXtProti NX_Q14691.
    PharmGKBi PA145008291.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5230.
    HOGENOMi HOG000192102.
    HOVERGENi HBG053743.
    InParanoidi Q14691.
    KOi K10732.
    OMAi LQRNKRC.
    OrthoDBi EOG7M6D8F.
    PhylomeDBi Q14691.
    TreeFami TF312848.

    Enzyme and pathway databases

    Reactomei REACT_6776. Unwinding of DNA.

    Miscellaneous databases

    ChiTaRSi GINS1. human.
    EvolutionaryTracei Q14691.
    GeneWikii GINS1.
    GenomeRNAii 9837.
    NextBioi 37066.
    PROi Q14691.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14691.
    Bgeei Q14691.
    CleanExi HS_GINS1.
    Genevestigatori Q14691.

    Family and domain databases

    InterProi IPR005339. GINS_Psf1.
    [Graphical view ]
    PANTHERi PTHR12914. PTHR12914. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."
      Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N.
      DNA Res. 3:17-24(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow.
    2. "The DNA sequence and comparative analysis of human chromosome 20."
      Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
      , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
      Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-97.
      Tissue: Skin.
    4. "Comprehensive expression profiling of tumor cell lines identifies molecular signatures of melanoma progression."
      Ryu B., Kim D.S., Deluca A.M., Alani R.M.
      PLoS ONE 2:E594-E594(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (33 ANGSTROMS) IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT, MASS SPECTROMETRY OF GINS COMPLEX.
    8. "Crystal structure of the human GINS complex."
      Choi J.M., Lim H.S., Kim J.J., Song O.K., Cho Y.
      Genes Dev. 21:1316-1321(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-151 IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT.
    9. "Structure of the human GINS complex and its assembly and functional interface in replication initiation."
      Kamada K., Kubota Y., Arata T., Shindo Y., Hanaoka F.
      Nat. Struct. Mol. Biol. 14:388-396(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-149 IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT.
    10. "Crystal structure of the GINS complex and functional insights into its role in DNA replication."
      Chang Y.P., Wang G., Bermudez V., Hurwitz J., Chen X.S.
      Proc. Natl. Acad. Sci. U.S.A. 104:12685-12690(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.36 ANGSTROMS) IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT, REGION.

    Entry informationi

    Entry nameiPSF1_HUMAN
    AccessioniPrimary (citable) accession number: Q14691
    Secondary accession number(s): Q9NQE2, Q9NQI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 20
      Human chromosome 20: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3