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Q14691

- PSF1_HUMAN

UniProt

Q14691 - PSF1_HUMAN

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Protein
DNA replication complex GINS protein PSF1
Gene
GINS1, KIAA0186, PSF1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS1 is essential for function.1 Publication

GO - Biological processi

  1. DNA strand elongation involved in DNA replication Source: Reactome
  2. inner cell mass cell proliferation Source: Ensembl
  3. mitotic cell cycle Source: Reactome
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_6776. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein PSF1
Alternative name(s):
GINS complex subunit 1
Gene namesi
Name:GINS1
Synonyms:KIAA0186, PSF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:28980. GINS1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleoplasm Source: Reactome
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA145008291.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196DNA replication complex GINS protein PSF1
PRO_0000219035Add
BLAST

Proteomic databases

MaxQBiQ14691.
PaxDbiQ14691.
PeptideAtlasiQ14691.
PRIDEiQ14691.

PTM databases

PhosphoSiteiQ14691.

Expressioni

Inductioni

Significantly up-regulated in aggressive melanomas.1 Publication

Gene expression databases

ArrayExpressiQ14691.
BgeeiQ14691.
CleanExiHS_GINS1.
GenevestigatoriQ14691.

Organism-specific databases

HPAiHPA051185.
HPA054486.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS4. GINS complex interacts with DNA primase in vitro.4 Publications

Protein-protein interaction databases

BioGridi115174. 5 interactions.
DIPiDIP-29331N.
STRINGi9606.ENSP00000262460.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1512
Beta strandi16 – 194
Helixi26 – 4924
Helixi56 – 583
Helixi59 – 9436
Helixi100 – 1045
Helixi108 – 12720
Beta strandi128 – 1325

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30A/E1-149[»]
2EHOX-ray3.00B/F/J1-151[»]
2Q9QX-ray2.36C/G1-196[»]
ProteinModelPortaliQ14691.
SMRiQ14691. Positions 1-144.

Miscellaneous databases

EvolutionaryTraceiQ14691.

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS1/PSF1 family.

Phylogenomic databases

eggNOGiCOG5230.
HOGENOMiHOG000192102.
HOVERGENiHBG053743.
InParanoidiQ14691.
KOiK10732.
OMAiLQRNKRC.
OrthoDBiEOG7M6D8F.
PhylomeDBiQ14691.
TreeFamiTF312848.

Family and domain databases

InterProiIPR005339. GINS_Psf1.
[Graphical view]
PANTHERiPTHR12914. PTHR12914. 1 hit.

Sequencei

Sequence statusi: Complete.

Q14691-1 [UniParc]FASTAAdd to Basket

« Hide

MFCEKAMELI RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA    50
KSGGRSDLIP TIKFRHCSLL RNRRCTVAYL YDRLLRIRAL RWEYGSVLPN 100
ALRFHMAAEE MEWFNNYKRS LATYMRSLGG DEGLDITQDM KPPKSLYIEV 150
RCLKDYGEFE VDDGTSVLLK KNSQHFLPRW KCEQLIRQGV LEHILS 196
Length:196
Mass (Da):22,988
Last modified:November 1, 1996 - v1
Checksum:i2270B5CE6C43BFE5
GO

Sequence cautioni

The sequence BAA11503.2 differs from that shown. Reason: Erroneous initiation.

Mass spectrometryi

Molecular mass is 98373±13 Da from positions 1 - 196. Determined by ESI. This is the measured mass for the GINS complex.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti97 – 971V → I.1 Publication
Corresponds to variant rs6076347 [ dbSNP | Ensembl ].
VAR_051606

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D80008 mRNA. Translation: BAA11503.2. Different initiation.
AL353812, AL031672 Genomic DNA. Translation: CAI13764.1.
AL031672, AL353812 Genomic DNA. Translation: CAI43019.1.
BC012542 mRNA. Translation: AAH12542.1.
CCDSiCCDS33451.1.
RefSeqiNP_066545.3. NM_021067.3.
UniGeneiHs.658464.

Genome annotation databases

EnsembliENST00000262460; ENSP00000262460; ENSG00000101003.
GeneIDi9837.
KEGGihsa:9837.
UCSCiuc002wuv.1. human.

Polymorphism databases

DMDMi6226339.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D80008 mRNA. Translation: BAA11503.2 . Different initiation.
AL353812 , AL031672 Genomic DNA. Translation: CAI13764.1 .
AL031672 , AL353812 Genomic DNA. Translation: CAI43019.1 .
BC012542 mRNA. Translation: AAH12542.1 .
CCDSi CCDS33451.1.
RefSeqi NP_066545.3. NM_021067.3.
UniGenei Hs.658464.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E9X X-ray 2.30 A/E 1-149 [» ]
2EHO X-ray 3.00 B/F/J 1-151 [» ]
2Q9Q X-ray 2.36 C/G 1-196 [» ]
ProteinModelPortali Q14691.
SMRi Q14691. Positions 1-144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115174. 5 interactions.
DIPi DIP-29331N.
STRINGi 9606.ENSP00000262460.

PTM databases

PhosphoSitei Q14691.

Polymorphism databases

DMDMi 6226339.

Proteomic databases

MaxQBi Q14691.
PaxDbi Q14691.
PeptideAtlasi Q14691.
PRIDEi Q14691.

Protocols and materials databases

DNASUi 9837.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000262460 ; ENSP00000262460 ; ENSG00000101003 .
GeneIDi 9837.
KEGGi hsa:9837.
UCSCi uc002wuv.1. human.

Organism-specific databases

CTDi 9837.
GeneCardsi GC20P025389.
HGNCi HGNC:28980. GINS1.
HPAi HPA051185.
HPA054486.
MIMi 610608. gene.
neXtProti NX_Q14691.
PharmGKBi PA145008291.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5230.
HOGENOMi HOG000192102.
HOVERGENi HBG053743.
InParanoidi Q14691.
KOi K10732.
OMAi LQRNKRC.
OrthoDBi EOG7M6D8F.
PhylomeDBi Q14691.
TreeFami TF312848.

Enzyme and pathway databases

Reactomei REACT_6776. Unwinding of DNA.

Miscellaneous databases

ChiTaRSi GINS1. human.
EvolutionaryTracei Q14691.
GeneWikii GINS1.
GenomeRNAii 9837.
NextBioi 37066.
PROi Q14691.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q14691.
Bgeei Q14691.
CleanExi HS_GINS1.
Genevestigatori Q14691.

Family and domain databases

InterProi IPR005339. GINS_Psf1.
[Graphical view ]
PANTHERi PTHR12914. PTHR12914. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."
    Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N.
    DNA Res. 3:17-24(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-97.
    Tissue: Skin.
  4. "Comprehensive expression profiling of tumor cell lines identifies molecular signatures of melanoma progression."
    Ryu B., Kim D.S., Deluca A.M., Alani R.M.
    PLoS ONE 2:E594-E594(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (33 ANGSTROMS) IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT, MASS SPECTROMETRY OF GINS COMPLEX.
  8. "Crystal structure of the human GINS complex."
    Choi J.M., Lim H.S., Kim J.J., Song O.K., Cho Y.
    Genes Dev. 21:1316-1321(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-151 IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT.
  9. "Structure of the human GINS complex and its assembly and functional interface in replication initiation."
    Kamada K., Kubota Y., Arata T., Shindo Y., Hanaoka F.
    Nat. Struct. Mol. Biol. 14:388-396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-149 IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT.
  10. "Crystal structure of the GINS complex and functional insights into its role in DNA replication."
    Chang Y.P., Wang G., Bermudez V., Hurwitz J., Chen X.S.
    Proc. Natl. Acad. Sci. U.S.A. 104:12685-12690(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.36 ANGSTROMS) IN COMPLEX WITH GINS2; GINS3 AND GINS4, SUBUNIT, REGION.

Entry informationi

Entry nameiPSF1_HUMAN
AccessioniPrimary (citable) accession number: Q14691
Secondary accession number(s): Q9NQE2, Q9NQI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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