Reviewed, UniProtKB/Swiss-Prot Q14684 (RRP1B_HUMAN)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA processing protein 1 homolog B
Alternative name(s):
    RRP1-like protein B

Gene names

Name:	RRP1B
Synonyms:	KIAA0179

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Nucleus › nucleolus.
Sequence similarities	Belongs to the RRP1 family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB nucleus Inferred from direct assay. Source: UniProtKB preribosome, small subunit precursor Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSM1	O15116	1	EBI-372051,EBI-347619

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q14684-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14684-2)

The sequence of this isoform differs from the canonical sequence as follows:
     51-68: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	Ribosomal RNA processing protein 1 homolog B		PRO_0000050729
Regions
	Compositional bias	402 – 406	5	Poly-Lys
Amino acid modifications
	Modified residue	245	1	Phosphoserine
	Modified residue	350	1	Phosphoserine
	Modified residue	392	1	Phosphoserine
	Modified residue	394	1	Phosphoserine
	Modified residue	395	1	Phosphoserine
	Modified residue	452	1	Phosphoserine
	Modified residue	453	1	Phosphoserine
	Modified residue	458	1	Phosphoserine
	Modified residue	513	1	Phosphoserine
	Modified residue	702	1	Phosphoserine
	Modified residue	706	1	Phosphoserine
	Modified residue	732	1	Phosphoserine
	Modified residue	736	1	Phosphoserine
	Modified residue	747	1	Phosphothreonine
Natural variations
	Alternative sequence	51 – 68	18	Missing in isoform 2.		VSP_007801

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2003. Version 2. Checksum: 22CE71CF47641AD4	FASTA	758	84,411
	10 20 30 40 50 60 MAPAMQPAEI QFAQRLASSE KGIRDRAVKK LRQYISVKTQ RETGGFSQEE LLKIWKGLFY 70 80 90 100 110 120 CMWVQDEPLL QEELANTIAQ LVHAVNNSAA QHLFIQTFWQ TMNREWKGID RLRLDKYYML 130 140 150 160 170 180 IRLVLRQSFE VLKRNGWEES RIKVFLDVLM KEVLCPESQS PNGVRFHFID IYLDELSKVG 190 200 210 220 230 240 GKELLADQNL KFIDPFCKIA AKTKDHTLVQ TIARGVFEAI VDQSPFVPEE TMEEQKTKVG 250 260 270 280 290 300 DGDLSAEEIP ENEVSLRRAV SKKKTALGKN HSRKDGLSDE RGRDDCGTFE DTGPLLQFDY 310 320 330 340 350 360 KAVADRLLEM TSRKNTPHFN RKRLSKLIKK FQDLSEGSSI SQLSFAEDIS ADEDDQILSQ 370 380 390 400 410 420 GKHKKKGNKL LEKTNLEKEK GSRVFCVEEE DSESSLQKRR RKKKKKHHLQ PENPGPGGAA 430 440 450 460 470 480 PSLEQNRGRE PEASGPKALK ARVAEPGAEA TSSTGEESGS EHPPAVPMHN KRKRPRKKSP 490 500 510 520 530 540 RAHREMLESA VLPPEDMSQS GPSGSHPQGP RGSPTGGAQL LKRKRKLGVV PVNGSGLSTP 550 560 570 580 590 600 AWPPLQQEGP PTGPAEGANS HTTLPQRRRL QKKKAGPGSL ELCGLPSQKT ASLKKRKKMR 610 620 630 640 650 660 VMSNLVEHNG VLESEAGQPQ ALGSSGTCSS LKKQKLRAES DFVKFDTPFL PKPLFFRRAK 670 680 690 700 710 720 SSTATHPPGP AVQLNKTPSS SKKVTFGLNR NMTAEFKKTD KSILVSPTGP SRVAFDPEQK 730 740 750 PLHGVLKTPT SSPASSPLVA KKPLTTTPRR RPRAMDFF
	Isoform 2 [UniParc]. Checksum: 2C76AD1FEB0FAC73		740	82,160
	10 20 30 40 50 60 MAPAMQPAEI QFAQRLASSE KGIRDRAVKK LRQYISVKTQ RETGGFSQEE LLQEELANTI 70 80 90 100 110 120 AQLVHAVNNS AAQHLFIQTF WQTMNREWKG IDRLRLDKYY MLIRLVLRQS FEVLKRNGWE 130 140 150 160 170 180 ESRIKVFLDV LMKEVLCPES QSPNGVRFHF IDIYLDELSK VGGKELLADQ NLKFIDPFCK 190 200 210 220 230 240 IAAKTKDHTL VQTIARGVFE AIVDQSPFVP EETMEEQKTK VGDGDLSAEE IPENEVSLRR 250 260 270 280 290 300 AVSKKKTALG KNHSRKDGLS DERGRDDCGT FEDTGPLLQF DYKAVADRLL EMTSRKNTPH 310 320 330 340 350 360 FNRKRLSKLI KKFQDLSEGS SISQLSFAED ISADEDDQIL SQGKHKKKGN KLLEKTNLEK 370 380 390 400 410 420 EKGSRVFCVE EEDSESSLQK RRRKKKKKHH LQPENPGPGG AAPSLEQNRG REPEASGPKA 430 440 450 460 470 480 LKARVAEPGA EATSSTGEES GSEHPPAVPM HNKRKRPRKK SPRAHREMLE SAVLPPEDMS 490 500 510 520 530 540 QSGPSGSHPQ GPRGSPTGGA QLLKRKRKLG VVPVNGSGLS TPAWPPLQQE GPPTGPAEGA 550 560 570 580 590 600 NSHTTLPQRR RLQKKKAGPG SLELCGLPSQ KTASLKKRKK MRVMSNLVEH NGVLESEAGQ 610 620 630 640 650 660 PQALGSSGTC SSLKKQKLRA ESDFVKFDTP FLPKPLFFRR AKSSTATHPP GPAVQLNKTP 670 680 690 700 710 720 SSSKKVTFGL NRNMTAEFKK TDKSILVSPT GPSRVAFDPE QKPLHGVLKT PTSSPASSPL 730 740 VAKKPLTTTP RRRPRAMDFF

References

[1]	"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N. DNA Res. 3:17-24(1996) [PubMed: 8724849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Bone marrow.
[2]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed: 10830953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed: 12429849] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392; SER-513 AND SER-706, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-732, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513 AND THR-747, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245; SER-350; SER-392; SER-394; SER-395; SER-452; SER-453; SER-458; SER-702; SER-706; SER-732 AND SER-736, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D80001 mRNA. Translation: BAA11496.1. Different initiation. AP001052 Genomic DNA. No translation available. BC028386 mRNA. Translation: AAH28386.1.
RefSeq	NP_055871.1.
UniGene	Hs.654727
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14684.
PTM databases
PhosphoSite	Q14684.
2-D gel databases
SWISS-2DPAGE	Q14684.
Genome annotation databases
Ensembl	ENSG00000160208. Homo sapiens. [Contig view]
GeneID	23076.
KEGG	hsa:23076.
Organism-specific databases
HGNC	HGNC:23818. RRP1B.
MIM	610654. gene.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q14684.
Gene expression databases
ArrayExpress	Q14684.
CleanEx	HS_RRP1B.
GermOnline	ENSG00000160208. Homo sapiens.
Family and domain databases
InterPro	IPR010301. Nop52. [Graphical view]
PANTHER	PTHR13026. Nop52. 1 hit.
Pfam	PF05997. Nop52. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44187.
SOURCE	Search...

Entry information

Entry name

RRP1B_HUMAN

Accession

Primary (citable) accession number: Q14684
Secondary accession number(s): Q8TBZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 19, 2003
Last modified:	November 25, 2008
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)