Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Clathrin interactor 1

Gene

CLINT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei67Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • clathrin binding Source: BHF-UCL
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000113282-MONOMER.
ReactomeiR-HSA-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin interactor 1
Alternative name(s):
Clathrin-interacting protein localized in the trans-Golgi region
Short name:
Clint
Enthoprotin
Epsin-4
Epsin-related protein
Short name:
EpsinR
Gene namesi
Name:CLINT1
Synonyms:ENTH, EPN4, EPNR, KIAA0171
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:23186. CLINT1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • clathrin-coated vesicle Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • Golgi apparatus Source: UniProtKB
  • intracellular membrane-bounded organelle Source: HPA
  • membrane Source: UniProtKB
  • nucleoplasm Source: HPA
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi29R → L: Reduces lipid binding. Abolishes lipid binding; when associated with G-34. 1
Mutagenesisi34D → G: Abolishes lipid binding; when associated with L-29. 1 Publication1
Mutagenesisi349D → R: Decreases AP-1 and AP-2 binding. 1 Publication1
Mutagenesisi371D → R: Slightly decreases AP-1 binding. 1 Publication1
Mutagenesisi422D → R: Strongly decreases clathrin binding. 1 Publication1
Mutagenesisi423 – 426LFDL → AFAA: Strongly reduces clathrin binding. 1 Publication4

Organism-specific databases

DisGeNETi9685.
OpenTargetsiENSG00000113282.
Orphaneti3140. Schizophrenia.
PharmGKBiPA145149115.

Polymorphism and mutation databases

BioMutaiCLINT1.
DMDMi41016993.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745211 – 625Clathrin interactor 1Add BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei163PhosphoserineBy similarity1
Modified residuei166PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei210PhosphoserineCombined sources1
Modified residuei227PhosphoserineCombined sources1
Modified residuei245PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei624PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14677.
MaxQBiQ14677.
PaxDbiQ14677.
PeptideAtlasiQ14677.
PRIDEiQ14677.

PTM databases

iPTMnetiQ14677.
PhosphoSitePlusiQ14677.

Expressioni

Tissue specificityi

Ubiquitously expressed at low to intermediate levels.2 Publications

Gene expression databases

BgeeiENSG00000113282.
CleanExiHS_CLINT1.
ExpressionAtlasiQ14677. baseline and differential.
GenevisibleiQ14677. HS.

Organism-specific databases

HPAiHPA043280.
HPA056947.

Interactioni

Subunit structurei

Binds clathrin heavy chain, GGA2, AP-2 and AP1G1.

Binary interactionsi

WithEntry#Exp.IntActNotes
Ap1g1P2289210EBI-1171113,EBI-1040262From a different organism.
GABARAPL1Q9H0R82EBI-1171113,EBI-746969
MAP1LC3BQ9GZQ82EBI-1171113,EBI-373144

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • clathrin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi115038. 97 interactors.
DIPiDIP-45604N.
IntActiQ14677. 64 interactors.
MINTiMINT-4999242.
STRINGi9606.ENSP00000429824.

Structurei

Secondary structure

1625
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 31Combined sources9
Beta strandi33 – 37Combined sources5
Helixi41 – 50Combined sources10
Turni54 – 56Combined sources3
Helixi57 – 69Combined sources13
Helixi76 – 92Combined sources17
Helixi95 – 103Combined sources9
Helixi105 – 109Combined sources5
Helixi110 – 113Combined sources4
Helixi125 – 139Combined sources15
Helixi142 – 151Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XGWX-ray1.90A1-165[»]
2QY7X-ray2.00A/B/C20-166[»]
2V8SX-ray2.22E20-166[»]
ProteinModelPortaliQ14677.
SMRiQ14677.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14677.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 149ENTHPROSITE-ProRule annotationAdd BLAST134

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi549 – 605Met-richAdd BLAST57

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000082415.
HOVERGENiHBG048921.
InParanoidiQ14677.
OMAiNMLPVRP.
OrthoDBiEOG091G0FWU.
PhylomeDBiQ14677.
TreeFamiTF313361.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR030544. CLINT1.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR12276:SF53. PTHR12276:SF53. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14677-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLNMWKVREL VDKATNVVMN YSEIESKVRE ATNDDPWGPS GQLMGEIAKA
60 70 80 90 100
TFMYEQFPEL MNMLWSRMLK DNKKNWRRVY KSLLLLAYLI RNGSERVVTS
110 120 130 140 150
AREHIYDLRS LENYHFVDEH GKDQGINIRQ KVKELVEFAQ DDDRLREERK
160 170 180 190 200
KAKKNKDKYV GVSSDSVGGF RYSERYDPEP KSKWDEEWDK NKSAFPFSDK
210 220 230 240 250
LGELSDKIGS TIDDTISKFR RKDREDSPER CSDSDEEKKA RRGRSPKGEF
260 270 280 290 300
KDEEETVTTK HIHITQATET TTTRHKRTAN PSKTIDLGAA AHYTGDKASP
310 320 330 340 350
DQNASTHTPQ SSVKTSVPSS KSSGDLVDLF DGTSQSTGGS ADLFGGFADF
360 370 380 390 400
GSAAASGSFP SQVTATSGNG DFGDWSAFNQ APSGPVASSG EFFGSASQPA
410 420 430 440 450
VELVSGSQSA LGPPPAASNS SDLFDLMGSS QATMTSSQSM NFSMMSTNTV
460 470 480 490 500
GLGLPMSRSQ NTDMVQKSVS KTLPSTWSDP SVNISLDNLL PGMQPSKPQQ
510 520 530 540 550
PSLNTMIQQQ NMQQPMNVMT QSFGAVNLSS PSNMLPVRPQ TNALIGGPMP
560 570 580 590 600
MSMPNVMTGT MGMAPLGNTP MMNQSMMGMN MNIGMSAAGM GLTGTMGMGM
610 620
PNIAMTSGTV QPKQDAFANF ANFSK
Length:625
Mass (Da):68,259
Last modified:November 1, 1996 - v1
Checksum:i6C8C6689861E9F0D
GO
Isoform 2 (identifier: Q14677-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: Missing.
     460-460: Q → QPLQNVSTVLQKPNPLYNQ

Show »
Length:625
Mass (Da):68,166
Checksum:i5081B36E2F4F85F2
GO
Isoform 3 (identifier: Q14677-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-459: S → SQPLQNVSTVLQKPNPLYN

Note: No experimental confirmation available.
Show »
Length:643
Mass (Da):70,295
Checksum:iFD6381C2E1957E9C
GO

Sequence cautioni

The sequence BAA11488 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti476T → A in BAC03971 (PubMed:14702039).Curated1

Polymorphismi

Genetic variations in CLINT1 may contribute to susceptibility to schizophrenia (SCZD1) and psychotic disorders in some populations.1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0091601 – 18Missing in isoform 2. 2 PublicationsAdd BLAST18
Alternative sequenceiVSP_043302459S → SQPLQNVSTVLQKPNPLYN in isoform 3. 1 Publication1
Alternative sequenceiVSP_009161460Q → QPLQNVSTVLQKPNPLYNQ in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK000414 Genomic DNA. Translation: DAA00062.1.
AF434813 mRNA. Translation: AAL30768.1.
D79993 mRNA. Translation: BAA11488.2. Different initiation.
AK092765 mRNA. Translation: BAC03971.1.
AK300257 mRNA. Translation: BAH13244.1.
AC011394 Genomic DNA. No translation available.
AC026407 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61585.1.
CH471062 Genomic DNA. Translation: EAW61588.1.
BC004467 mRNA. Translation: AAH04467.1.
BC013091 mRNA. Translation: AAH13091.1.
CCDSiCCDS47330.1. [Q14677-1]
CCDS56388.1. [Q14677-2]
CCDS56389.1. [Q14677-3]
RefSeqiNP_001182484.1. NM_001195555.1. [Q14677-3]
NP_001182485.1. NM_001195556.1. [Q14677-2]
NP_055481.1. NM_014666.3. [Q14677-1]
UniGeneiHs.644000.

Genome annotation databases

EnsembliENST00000411809; ENSP00000388340; ENSG00000113282. [Q14677-1]
ENST00000523094; ENSP00000429345; ENSG00000113282. [Q14677-2]
ENST00000523908; ENSP00000429824; ENSG00000113282. [Q14677-3]
ENST00000530742; ENSP00000433419; ENSG00000113282. [Q14677-2]
GeneIDi9685.
KEGGihsa:9685.
UCSCiuc003lxi.3. human. [Q14677-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK000414 Genomic DNA. Translation: DAA00062.1.
AF434813 mRNA. Translation: AAL30768.1.
D79993 mRNA. Translation: BAA11488.2. Different initiation.
AK092765 mRNA. Translation: BAC03971.1.
AK300257 mRNA. Translation: BAH13244.1.
AC011394 Genomic DNA. No translation available.
AC026407 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61585.1.
CH471062 Genomic DNA. Translation: EAW61588.1.
BC004467 mRNA. Translation: AAH04467.1.
BC013091 mRNA. Translation: AAH13091.1.
CCDSiCCDS47330.1. [Q14677-1]
CCDS56388.1. [Q14677-2]
CCDS56389.1. [Q14677-3]
RefSeqiNP_001182484.1. NM_001195555.1. [Q14677-3]
NP_001182485.1. NM_001195556.1. [Q14677-2]
NP_055481.1. NM_014666.3. [Q14677-1]
UniGeneiHs.644000.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XGWX-ray1.90A1-165[»]
2QY7X-ray2.00A/B/C20-166[»]
2V8SX-ray2.22E20-166[»]
ProteinModelPortaliQ14677.
SMRiQ14677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115038. 97 interactors.
DIPiDIP-45604N.
IntActiQ14677. 64 interactors.
MINTiMINT-4999242.
STRINGi9606.ENSP00000429824.

PTM databases

iPTMnetiQ14677.
PhosphoSitePlusiQ14677.

Polymorphism and mutation databases

BioMutaiCLINT1.
DMDMi41016993.

Proteomic databases

EPDiQ14677.
MaxQBiQ14677.
PaxDbiQ14677.
PeptideAtlasiQ14677.
PRIDEiQ14677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000411809; ENSP00000388340; ENSG00000113282. [Q14677-1]
ENST00000523094; ENSP00000429345; ENSG00000113282. [Q14677-2]
ENST00000523908; ENSP00000429824; ENSG00000113282. [Q14677-3]
ENST00000530742; ENSP00000433419; ENSG00000113282. [Q14677-2]
GeneIDi9685.
KEGGihsa:9685.
UCSCiuc003lxi.3. human. [Q14677-1]

Organism-specific databases

CTDi9685.
DisGeNETi9685.
GeneCardsiCLINT1.
HGNCiHGNC:23186. CLINT1.
HPAiHPA043280.
HPA056947.
MIMi607265. gene.
neXtProtiNX_Q14677.
OpenTargetsiENSG00000113282.
Orphaneti3140. Schizophrenia.
PharmGKBiPA145149115.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000082415.
HOVERGENiHBG048921.
InParanoidiQ14677.
OMAiNMLPVRP.
OrthoDBiEOG091G0FWU.
PhylomeDBiQ14677.
TreeFamiTF313361.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000113282-MONOMER.
ReactomeiR-HSA-432722. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiCLINT1. human.
EvolutionaryTraceiQ14677.
GeneWikiiCLINT1.
GenomeRNAii9685.
PROiQ14677.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113282.
CleanExiHS_CLINT1.
ExpressionAtlasiQ14677. baseline and differential.
GenevisibleiQ14677. HS.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR030544. CLINT1.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR12276:SF53. PTHR12276:SF53. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPN4_HUMAN
AccessioniPrimary (citable) accession number: Q14677
Secondary accession number(s): B7Z6F8
, D3DQJ6, Q8NAF1, Q96E05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.