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Q14657 (LAGE3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
EKC/KEOPS complex subunit LAGE3
Alternative name(s):
L antigen family member 3
Protein ESO-3
Protein ITBA2
Gene names
Name:LAGE3
Synonyms:DXS9879E, ESO3, ITBA2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length143 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. LAGE3 functions as a dimerization module for the complex By similarity.

Subunit structure

Component of the EKC/KEOPS complex composed of at least TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes. Ref.6

Subcellular location

Nucleus Ref.6.

Tissue specificity

Ubiquitous. Ref.3 Ref.4

Sequence similarities

Belongs to the CTAG/PCC1 family.

Sequence caution

The sequence CAA63489.1 differs from that shown. Reason: Frameshift at position 54.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 143143EKC/KEOPS complex subunit LAGE3
PRO_0000218924

Sequences

Sequence LengthMass (Da)Tools
Q14657 [UniParc].

Last modified October 11, 2004. Version 2.
Checksum: AD164559371449F8

FASTA14314,804
        10         20         30         40         50         60 
MRDADADAGG GADGGDGRGG HSCRGGVDTA AAPAGGAPPA HAPGPGRDAA SAARGSRMRP 

        70         80         90        100        110        120 
HIFTLSVPFP TPLEAEIAHG SLAPDAEPHQ RVVGKDLTVS GRILVVRWKA EDCRLLRISV 

       130        140 
INFLDQLSLV VRTMQRFGPP VSR 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Blood and Pancreas.
[3]"Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach."
Faranda S., Frattini A., Zucchi I., Patrosso C., Milanesi L., Montagna C., Vezzoni P.
Genomics 34:323-327(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-143, TISSUE SPECIFICITY.
Tissue: Liver.
[4]"A new member of the NY-ESO-1 gene family is ubiquitously expressed in somatic tissues and evolutionarily conserved."
Alpen B., Guere A.O., Scanlan M.J., Old L.J., Chen Y.-T.
Gene 297:141-149(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"The human EKC/KEOPS complex is recruited to Cullin2 ubiquitin ligases by the human tumour antigen PRAME."
Costessi A., Mahrour N., Sharma V., Stunnenberg R., Stoel M.A., Tijchon E., Conaway J.W., Conaway R.C., Stunnenberg H.G.
PLoS ONE 7:E42822-E42822(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE EKC/KEOPS COMPLEX, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX936365 Genomic DNA. Translation: CAI43195.1.
BC015744 mRNA. Translation: AAH15744.2.
BC062330 mRNA. Translation: AAH62330.1.
X92896 mRNA. Translation: CAA63489.1. Frameshift.
CCDSCCDS14753.1.
RefSeqNP_006005.2. NM_006014.4.
UniGeneHs.444619.

3D structure databases

ProteinModelPortalQ14657.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113888. 35 interactions.
IntActQ14657. 4 interactions.
MINTMINT-4719865.
STRING9606.ENSP00000349923.

PTM databases

PhosphoSiteQ14657.

Polymorphism databases

DMDM54041570.

Proteomic databases

MaxQBQ14657.
PaxDbQ14657.
PeptideAtlasQ14657.
PRIDEQ14657.

Protocols and materials databases

DNASU8270.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000357360; ENSP00000349923; ENSG00000196976.
GeneID8270.
KEGGhsa:8270.
UCSCuc004fln.1. human.

Organism-specific databases

CTD8270.
GeneCardsGC0XM153707.
HGNCHGNC:26058. LAGE3.
HPAHPA036122.
HPA036123.
MIM300060. gene.
neXtProtNX_Q14657.
PharmGKBPA128394540.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40896.
HOGENOMHOG000040003.
HOVERGENHBG052154.
InParanoidQ14657.
KOK15902.
OMAWTAKDPR.
PhylomeDBQ14657.
TreeFamTF337064.

Gene expression databases

ArrayExpressQ14657.
BgeeQ14657.
CleanExHS_LAGE3.
GenevestigatorQ14657.

Family and domain databases

InterProIPR015419. EKC/KEOPS_Pcc1.
[Graphical view]
PfamPF09341. Pcc1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi8270.
NextBio31037.
PMAP-CutDBQ14657.
PROQ14657.
SOURCESearch...

Entry information

Entry nameLAGE3_HUMAN
AccessionPrimary (citable) accession number: Q14657
Secondary accession number(s): Q5HY39, Q8IZ78
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM