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Q14644 (RASA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras GTPase-activating protein 3
Alternative name(s):
GAP1(IP4BP)
Ins P4-binding protein
Gene names
Name:RASA3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.

Subcellular location

Cell membrane.

Sequence similarities

Contains 1 Btk-type zinc finger.

Contains 2 C2 domains.

Contains 1 PH domain.

Contains 1 Ras-GAP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 834834Ras GTPase-activating protein 3
PRO_0000056642

Regions

Domain1 – 9696C2 1
Domain132 – 247116C2 2
Domain330 – 524195Ras-GAP
Domain576 – 677102PH
Zinc finger679 – 71537Btk-type

Amino acid modifications

Modified residue661Phosphotyrosine By similarity
Modified residue1101Phosphothreonine Ref.6
Modified residue8091Phosphoserine Ref.8
Modified residue8331Phosphoserine By similarity

Experimental info

Mutagenesis5991K → Q: No binding to IP4 and loss of plasma membrane localization.
Mutagenesis6001K → Q: No binding to IP4 and loss of plasma membrane localization.
Mutagenesis6011R → Q: No binding to IP4 and loss of plasma membrane localization.
Sequence conflict785 – 7862AG → RW in CAA61580. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q14644 [UniParc].

Last modified September 23, 2008. Version 3.
Checksum: 97C3467C957DA8A1

FASTA83495,699
        10         20         30         40         50         60 
MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT KIVEKSLCPF 

        70         80         90        100        110        120 
YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE DLQKYHNRDT WFQLQHVDAD 

       130        140        150        160        170        180 
SEVQGKVHLE LRLSEVITDT GVVCHKLATR IVECQGLPIV NGQCDPYATV TLAGPFRSEA 

       190        200        210        220        230        240 
KKTKVKRKTN NPQFDEVFYF EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD 

       250        260        270        280        290        300 
EFLGELRIPL KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY 

       310        320        330        340        350        360 
SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP FISAIASAEV 

       370        380        390        400        410        420 
KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI EEICQSHKPC EIDPVKLKDG 

       430        440        450        460        470        480 
ENLENNMENL RQYVDRVFHA ITESGVSCPT VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS 

       490        500        510        520        530        540 
FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT 

       550        560        570        580        590        600 
FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK 

       610        620        630        640        650        660 
RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ PERALYIQAN 

       670        680        690        700        710        720 
NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR APSDSAPGCS PCTGGLPANI 

       730        740        750        760        770        780 
QLDIDGDRET ERIYSLFNLY MSKLEKMQEA CGSKSVYDGP EQEEYSTFVI DDPQETYKTL 

       790        800        810        820        830 
KQVIAGVGAL EQEHAQYKRD KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a specific Ins(1,3,4,5)P4-binding protein as a member of the GAP1 family."
Cullen P.J., Hsuan J.J., Truong O., Letcher A.J., Jackson T.J., Dawson A.P., Irvine R.F.
Nature 376:527-530(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Peripheral blood.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Testis.
[5]"Distinct subcellular localisations of the putative inositol 1,3,4,5-tetrakisphosphate receptors GAP1(IP4BP) and GAP1m result from the GAP1(IP4BP) PH domain directing plasma membrane targeting."
Lockyer P.J., Bottomley J.R., Reynolds J.S., McNulty T.J., Venkateswarlu K., Potter B.V.L., Dempsey C.E., Cullen P.J.
Curr. Biol. 7:1007-1010(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
Tissue: Blood.
[6]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89399 mRNA. Translation: CAA61580.1.
AK289746 mRNA. Translation: BAF82435.1.
AL161774, BX537329 Genomic DNA. Translation: CAI12864.2.
BX537329, AL161774 Genomic DNA. Translation: CAM28365.1.
BC038456 mRNA. Translation: AAH38456.1.
BC047242 mRNA. Translation: AAH47242.1.
RefSeqNP_031394.2. NM_007368.2.
UniGeneHs.593075.

3D structure databases

ProteinModelPortalQ14644.
SMRQ14644. Positions 11-568, 577-713.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116497. 7 interactions.
STRING9606.ENSP00000335029.

PTM databases

PhosphoSiteQ14644.

Polymorphism databases

DMDM206729910.

Proteomic databases

PaxDbQ14644.
PRIDEQ14644.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000334062; ENSP00000335029; ENSG00000185989.
GeneID22821.
KEGGhsa:22821.
UCSCuc001vui.3. human.

Organism-specific databases

CTD22821.
GeneCardsGC13M114747.
HGNCHGNC:20331. RASA3.
HPAHPA046759.
MIM605182. gene.
neXtProtNX_Q14644.
PharmGKBPA134905447.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG323905.
HOGENOMHOG000007183.
HOVERGENHBG055643.
KOK12380.
OMAPICDIPL.
OrthoDBEOG7J17Z6.
PhylomeDBQ14644.
TreeFamTF105302.

Gene expression databases

ArrayExpressQ14644.
BgeeQ14644.
CleanExHS_RASA3.
GenevestigatorQ14644.

Family and domain databases

Gene3D1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProIPR000008. C2_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
PRINTSPR00402. TECBTKDOMAIN.
SMARTSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
PROSITEPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRASA3. human.
GeneWikiRASA3.
GenomeRNAi22821.
NextBio43216.
PROQ14644.
SOURCESearch...

Entry information

Entry nameRASA3_HUMAN
AccessionPrimary (citable) accession number: Q14644
Secondary accession number(s): A6NL15, Q8IUY2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM