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Protein

Ras GTPase-activating protein 3

Gene

RASA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri679 – 715Btk-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

  • calcium-release channel activity Source: BHF-UCL
  • GTPase activator activity Source: ProtInc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • MAPK cascade Source: Reactome
  • negative regulation of Ras protein signal transduction Source: GO_Central
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-32254-MONOMER.
ReactomeiR-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 3
Alternative name(s):
GAP1(IP4BP)
Ins P4-binding protein
Gene namesi
Name:RASA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:20331. RASA3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi599K → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication1
Mutagenesisi600K → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication1
Mutagenesisi601R → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication1

Organism-specific databases

DisGeNETi22821.
OpenTargetsiENSG00000185989.
ENSG00000280477.
PharmGKBiPA134905447.

Polymorphism and mutation databases

BioMutaiRASA3.
DMDMi206729910.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566421 – 834Ras GTPase-activating protein 3Add BLAST834

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphotyrosineBy similarity1
Modified residuei77PhosphoserineBy similarity1
Modified residuei110PhosphothreonineCombined sources1
Modified residuei809PhosphoserineCombined sources1
Modified residuei833PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14644.
MaxQBiQ14644.
PaxDbiQ14644.
PeptideAtlasiQ14644.
PRIDEiQ14644.

PTM databases

iPTMnetiQ14644.
PhosphoSitePlusiQ14644.

Expressioni

Gene expression databases

BgeeiENSG00000185989.
CleanExiHS_RASA3.
GenevisibleiQ14644. HS.

Organism-specific databases

HPAiHPA046759.

Interactioni

Protein-protein interaction databases

BioGridi116497. 8 interactors.
IntActiQ14644. 1 interactor.
STRINGi9606.ENSP00000335029.

Structurei

3D structure databases

ProteinModelPortaliQ14644.
SMRiQ14644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 96C2 1PROSITE-ProRule annotationAdd BLAST96
Domaini132 – 247C2 2PROSITE-ProRule annotationAdd BLAST116
Domaini330 – 524Ras-GAPPROSITE-ProRule annotationAdd BLAST195
Domaini576 – 677PHPROSITE-ProRule annotationAdd BLAST102

Sequence similaritiesi

Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri679 – 715Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2059. Eukaryota.
ENOG410Y128. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ14644.
KOiK12380.
OMAiSHKSCEI.
OrthoDBiEOG091G04T8.
PhylomeDBiQ14644.
TreeFamiTF105302.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 2 hits.
[Graphical view]
PRINTSiPR00402. TECBTKDOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14644-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT
60 70 80 90 100
KIVEKSLCPF YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE
110 120 130 140 150
DLQKYHNRDT WFQLQHVDAD SEVQGKVHLE LRLSEVITDT GVVCHKLATR
160 170 180 190 200
IVECQGLPIV NGQCDPYATV TLAGPFRSEA KKTKVKRKTN NPQFDEVFYF
210 220 230 240 250
EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD EFLGELRIPL
260 270 280 290 300
KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY
310 320 330 340 350
SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP
360 370 380 390 400
FISAIASAEV KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI
410 420 430 440 450
EEICQSHKPC EIDPVKLKDG ENLENNMENL RQYVDRVFHA ITESGVSCPT
460 470 480 490 500
VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS FIFLRFFAPA ILSPNLFQLT
510 520 530 540 550
PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT FYEFFNEQKY
560 570 580 590 600
ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK
610 620 630 640 650
RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ
660 670 680 690 700
PERALYIQAN NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR
710 720 730 740 750
APSDSAPGCS PCTGGLPANI QLDIDGDRET ERIYSLFNLY MSKLEKMQEA
760 770 780 790 800
CGSKSVYDGP EQEEYSTFVI DDPQETYKTL KQVIAGVGAL EQEHAQYKRD
810 820 830
KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI
Length:834
Mass (Da):95,699
Last modified:September 23, 2008 - v3
Checksum:i97C3467C957DA8A1
GO
Isoform 2 (identifier: Q14644-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: No experimental confirmation available.
Show »
Length:802
Mass (Da):92,228
Checksum:iA694D90C28A1F18D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti785 – 786AG → RW in CAA61580 (PubMed:7637787).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561411 – 32Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89399 mRNA. Translation: CAA61580.1.
AK289746 mRNA. Translation: BAF82435.1.
AK294913 mRNA. Translation: BAH11922.1.
AL161774, BX537329 Genomic DNA. Translation: CAI12864.2.
BX537329, AL161774 Genomic DNA. Translation: CAM28365.1.
BC038456 mRNA. Translation: AAH38456.1.
BC047242 mRNA. Translation: AAH47242.1.
CCDSiCCDS32016.1. [Q14644-1]
RefSeqiNP_001307750.1. NM_001320821.1.
NP_001307751.1. NM_001320822.1. [Q14644-2]
NP_031394.2. NM_007368.3. [Q14644-1]
XP_011533143.1. XM_011534841.2. [Q14644-2]
XP_011533144.1. XM_011534842.2. [Q14644-2]
UniGeneiHs.593075.

Genome annotation databases

EnsembliENST00000334062; ENSP00000335029; ENSG00000185989. [Q14644-1]
ENST00000630618; ENSP00000486064; ENSG00000280477. [Q14644-1]
GeneIDi22821.
KEGGihsa:22821.
UCSCiuc001vui.4. human. [Q14644-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89399 mRNA. Translation: CAA61580.1.
AK289746 mRNA. Translation: BAF82435.1.
AK294913 mRNA. Translation: BAH11922.1.
AL161774, BX537329 Genomic DNA. Translation: CAI12864.2.
BX537329, AL161774 Genomic DNA. Translation: CAM28365.1.
BC038456 mRNA. Translation: AAH38456.1.
BC047242 mRNA. Translation: AAH47242.1.
CCDSiCCDS32016.1. [Q14644-1]
RefSeqiNP_001307750.1. NM_001320821.1.
NP_001307751.1. NM_001320822.1. [Q14644-2]
NP_031394.2. NM_007368.3. [Q14644-1]
XP_011533143.1. XM_011534841.2. [Q14644-2]
XP_011533144.1. XM_011534842.2. [Q14644-2]
UniGeneiHs.593075.

3D structure databases

ProteinModelPortaliQ14644.
SMRiQ14644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116497. 8 interactors.
IntActiQ14644. 1 interactor.
STRINGi9606.ENSP00000335029.

PTM databases

iPTMnetiQ14644.
PhosphoSitePlusiQ14644.

Polymorphism and mutation databases

BioMutaiRASA3.
DMDMi206729910.

Proteomic databases

EPDiQ14644.
MaxQBiQ14644.
PaxDbiQ14644.
PeptideAtlasiQ14644.
PRIDEiQ14644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334062; ENSP00000335029; ENSG00000185989. [Q14644-1]
ENST00000630618; ENSP00000486064; ENSG00000280477. [Q14644-1]
GeneIDi22821.
KEGGihsa:22821.
UCSCiuc001vui.4. human. [Q14644-1]

Organism-specific databases

CTDi22821.
DisGeNETi22821.
GeneCardsiRASA3.
HGNCiHGNC:20331. RASA3.
HPAiHPA046759.
MIMi605182. gene.
neXtProtiNX_Q14644.
OpenTargetsiENSG00000185989.
ENSG00000280477.
PharmGKBiPA134905447.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2059. Eukaryota.
ENOG410Y128. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ14644.
KOiK12380.
OMAiSHKSCEI.
OrthoDBiEOG091G04T8.
PhylomeDBiQ14644.
TreeFamiTF105302.

Enzyme and pathway databases

BioCyciZFISH:G66-32254-MONOMER.
ReactomeiR-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

ChiTaRSiRASA3. human.
GeneWikiiRASA3.
GenomeRNAii22821.
PROiQ14644.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000185989.
CleanExiHS_RASA3.
GenevisibleiQ14644. HS.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 2 hits.
[Graphical view]
PRINTSiPR00402. TECBTKDOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASA3_HUMAN
AccessioniPrimary (citable) accession number: Q14644
Secondary accession number(s): A6NL15, F8W6X8, Q8IUY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.