Q14644 (RASA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating protein 3

Alternative name(s):
GAP1(IP4BP)
Ins P4-binding protein

Gene names

Name:

RASA3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.
Subcellular location	Cell membrane.
Sequence similarities	Contains 1 Btk-type zinc finger. Contains 2 C2 domains. Contains 1 PH domain. Contains 1 Ras-GAP domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro negative regulation of Ras protein signal transduction Inferred from Biological aspect of Ancestor. Source: RefGenome signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome intrinsic to internal side of plasma membrane Inferred from direct assay PubMed 10828023. Source: BHF-UCL
Molecular_function	Ras GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome calcium-release channel activity Inferred from direct assay PubMed 10828023. Source: BHF-UCL metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Ras GTPase-activating protein 3		PRO_0000056642
Regions
	Domain	1 – 96	96	C2 1
	Domain	132 – 247	116	C2 2
	Domain	330 – 524	195	Ras-GAP
	Domain	576 – 677	102	PH
	Zinc finger	679 – 715	37	Btk-type
Amino acid modifications
	Modified residue	66	1	Phosphotyrosine By similarity
	Modified residue	110	1	Phosphothreonine Ref.6
	Modified residue	809	1	Phosphoserine Ref.8
Experimental info
	Mutagenesis	599	1	K → Q: No binding to IP4 and loss of plasma membrane localization.
	Mutagenesis	600	1	K → Q: No binding to IP4 and loss of plasma membrane localization.
	Mutagenesis	601	1	R → Q: No binding to IP4 and loss of plasma membrane localization.
	Sequence conflict	785 – 786	2	AG → RW in CAA61580. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q14644 [UniParc]. Last modified September 23, 2008. Version 3. Checksum: 97C3467C957DA8A1	FASTA	834	95,699
	10 20 30 40 50 60 MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT KIVEKSLCPF 70 80 90 100 110 120 YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE DLQKYHNRDT WFQLQHVDAD 130 140 150 160 170 180 SEVQGKVHLE LRLSEVITDT GVVCHKLATR IVECQGLPIV NGQCDPYATV TLAGPFRSEA 190 200 210 220 230 240 KKTKVKRKTN NPQFDEVFYF EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD 250 260 270 280 290 300 EFLGELRIPL KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY 310 320 330 340 350 360 SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP FISAIASAEV 370 380 390 400 410 420 KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI EEICQSHKPC EIDPVKLKDG 430 440 450 460 470 480 ENLENNMENL RQYVDRVFHA ITESGVSCPT VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS 490 500 510 520 530 540 FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT 550 560 570 580 590 600 FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK 610 620 630 640 650 660 RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ PERALYIQAN 670 680 690 700 710 720 NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR APSDSAPGCS PCTGGLPANI 730 740 750 760 770 780 QLDIDGDRET ERIYSLFNLY MSKLEKMQEA CGSKSVYDGP EQEEYSTFVI DDPQETYKTL 790 800 810 820 830 KQVIAGVGAL EQEHAQYKRD KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a specific Ins(1,3,4,5)P4-binding protein as a member of the GAP1 family." Cullen P.J., Hsuan J.J., Truong O., Letcher A.J., Jackson T.J., Dawson A.P., Irvine R.F. Nature 376:527-530(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Testis.
[5]	"Distinct subcellular localisations of the putative inositol 1,3,4,5-tetrakisphosphate receptors GAP1(IP4BP) and GAP1m result from the GAP1(IP4BP) PH domain directing plasma membrane targeting." Lockyer P.J., Bottomley J.R., Reynolds J.S., McNulty T.J., Venkateswarlu K., Potter B.V.L., Dempsey C.E., Cullen P.J. Curr. Biol. 7:1007-1010(1997) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS. Tissue: Blood.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-110, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Platelet.
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89399 mRNA. Translation: CAA61580.1. AK289746 mRNA. Translation: BAF82435.1. AL161774, BX537329 Genomic DNA. Translation: CAI12864.2. BX537329, AL161774 Genomic DNA. Translation: CAM28365.1. BC038456 mRNA. Translation: AAH38456.1. BC047242 mRNA. Translation: AAH47242.1.
IPI	IPI01018718.
RefSeq	NP_031394.2. NM_007368.2.
UniGene	Hs.593075.
3D structure databases
ProteinModelPortal	Q14644.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000335029.
PTM databases
PhosphoSite	Q14644.
Polymorphism databases
DMDM	206729910.
Proteomic databases
PaxDb	Q14644.
PRIDE	Q14644.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334062; ENSP00000335029; ENSG00000185989.
GeneID	22821.
KEGG	hsa:22821.
UCSC	uc001vui.3. human.
Organism-specific databases
CTD	22821.
GeneCards	GC13M114747.
HGNC	HGNC:20331. RASA3.
HPA	HPA046759.
MIM	605182. gene.
neXtProt	NX_Q14644.
PharmGKB	PA134905447.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG323905.
HOGENOM	HOG000007183.
HOVERGEN	HBG055643.
KO	K12380.
OMA	APIGDDN.
OrthoDB	EOG4D7Z50.
Gene expression databases
ArrayExpress	Q14644.
Bgee	Q14644.
CleanEx	HS_RASA3.
Genevestigator	Q14644.
GermOnline	ENSG00000185989. Homo sapiens.
Family and domain databases
Gene3D	1.10.506.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. IPR001562. Znf_Btk_motif. [Graphical view]
Pfam	PF00779. BTK. 1 hit. PF00168. C2. 2 hits. PF00169. PH. 1 hit. PF00616. RasGAP. 1 hit. [Graphical view]
PRINTS	PR00402. TECBTKDOMAIN.
SMART	SM00107. BTK. 1 hit. SM00239. C2. 2 hits. SM00233. PH. 1 hit. SM00323. RasGAP. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 2 hits. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50004. C2. 2 hits. PS50003. PH_DOMAIN. 1 hit. PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. PS51113. ZF_BTK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RASA3. human.
GenomeRNAi	22821.
NextBio	43216.
SOURCE	Search...

Entry information

Entry name

RASA3_HUMAN

Accession

Primary (citable) accession number: Q14644
Secondary accession number(s): A6NL15, Q8IUY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.