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Protein

Ras GTPase-activating protein 3

Gene

RASA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri679 – 715Btk-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

  • calcium-release channel activity Source: BHF-UCL
  • GTPase activator activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • MAPK cascade Source: Reactome
  • negative regulation of Ras protein signal transduction Source: GO_Central
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5658442 Regulation of RAS by GAPs
R-HSA-6802949 Signaling by RAS mutants

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 3
Alternative name(s):
GAP1(IP4BP)
Ins P4-binding protein
Gene namesi
Name:RASA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000185989.10
HGNCiHGNC:20331 RASA3
MIMi605182 gene
neXtProtiNX_Q14644

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi599K → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication1
Mutagenesisi600K → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication1
Mutagenesisi601R → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication1

Organism-specific databases

DisGeNETi22821
OpenTargetsiENSG00000185989
PharmGKBiPA134905447

Polymorphism and mutation databases

BioMutaiRASA3
DMDMi206729910

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000566422 – 834Ras GTPase-activating protein 3Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei66PhosphotyrosineBy similarity1
Modified residuei77PhosphoserineBy similarity1
Modified residuei110PhosphothreonineCombined sources1
Modified residuei809PhosphoserineCombined sources1
Modified residuei833PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ14644
MaxQBiQ14644
PaxDbiQ14644
PeptideAtlasiQ14644
PRIDEiQ14644

PTM databases

iPTMnetiQ14644
PhosphoSitePlusiQ14644

Expressioni

Gene expression databases

BgeeiENSG00000185989
CleanExiHS_RASA3
GenevisibleiQ14644 HS

Organism-specific databases

HPAiHPA046759

Interactioni

Protein-protein interaction databases

BioGridi116497, 10 interactors
IntActiQ14644, 2 interactors
STRINGi9606.ENSP00000335029

Structurei

3D structure databases

ProteinModelPortaliQ14644
SMRiQ14644
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 96C2 1PROSITE-ProRule annotationAdd BLAST96
Domaini132 – 247C2 2PROSITE-ProRule annotationAdd BLAST116
Domaini330 – 524Ras-GAPPROSITE-ProRule annotationAdd BLAST195
Domaini576 – 677PHPROSITE-ProRule annotationAdd BLAST102

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri679 – 715Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2059 Eukaryota
ENOG410Y128 LUCA
GeneTreeiENSGT00760000119092
HOGENOMiHOG000007183
HOVERGENiHBG055643
InParanoidiQ14644
KOiK12380
OMAiHKSCEID
OrthoDBiEOG091G04T8
PhylomeDBiQ14644
TreeFamiTF105302

Family and domain databases

CDDicd13371 PH_GAP1_mammal-like, 1 hit
Gene3Di2.30.29.30, 1 hit
2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR037774 RASA3_PH
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
IPR001562 Znf_Btk_motif
PfamiView protein in Pfam
PF00779 BTK, 1 hit
PF00168 C2, 2 hits
PF00169 PH, 1 hit
PF00616 RasGAP, 2 hits
PRINTSiPR00402 TECBTKDOMAIN
SMARTiView protein in SMART
SM00107 BTK, 1 hit
SM00239 C2, 2 hits
SM00233 PH, 1 hit
SM00323 RasGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit
PS51113 ZF_BTK, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14644-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT
60 70 80 90 100
KIVEKSLCPF YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE
110 120 130 140 150
DLQKYHNRDT WFQLQHVDAD SEVQGKVHLE LRLSEVITDT GVVCHKLATR
160 170 180 190 200
IVECQGLPIV NGQCDPYATV TLAGPFRSEA KKTKVKRKTN NPQFDEVFYF
210 220 230 240 250
EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD EFLGELRIPL
260 270 280 290 300
KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY
310 320 330 340 350
SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP
360 370 380 390 400
FISAIASAEV KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI
410 420 430 440 450
EEICQSHKPC EIDPVKLKDG ENLENNMENL RQYVDRVFHA ITESGVSCPT
460 470 480 490 500
VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS FIFLRFFAPA ILSPNLFQLT
510 520 530 540 550
PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT FYEFFNEQKY
560 570 580 590 600
ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK
610 620 630 640 650
RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ
660 670 680 690 700
PERALYIQAN NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR
710 720 730 740 750
APSDSAPGCS PCTGGLPANI QLDIDGDRET ERIYSLFNLY MSKLEKMQEA
760 770 780 790 800
CGSKSVYDGP EQEEYSTFVI DDPQETYKTL KQVIAGVGAL EQEHAQYKRD
810 820 830
KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI
Length:834
Mass (Da):95,699
Last modified:September 23, 2008 - v3
Checksum:i97C3467C957DA8A1
GO
Isoform 2 (identifier: Q14644-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: No experimental confirmation available.
Show »
Length:802
Mass (Da):92,228
Checksum:iA694D90C28A1F18D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti785 – 786AG → RW in CAA61580 (PubMed:7637787).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561411 – 32Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89399 mRNA Translation: CAA61580.1
AK289746 mRNA Translation: BAF82435.1
AK294913 mRNA Translation: BAH11922.1
AL161774, BX537329 Genomic DNA Translation: CAI12864.2
BX537329, AL161774 Genomic DNA Translation: CAM28365.1
BC038456 mRNA Translation: AAH38456.1
BC047242 mRNA Translation: AAH47242.1
CCDSiCCDS32016.1 [Q14644-1]
RefSeqiNP_001307750.1, NM_001320821.1
NP_001307751.1, NM_001320822.1 [Q14644-2]
NP_031394.2, NM_007368.3 [Q14644-1]
XP_011533143.1, XM_011534841.2 [Q14644-2]
XP_011533144.1, XM_011534842.2 [Q14644-2]
UniGeneiHs.593075

Genome annotation databases

EnsembliENST00000334062; ENSP00000335029; ENSG00000185989 [Q14644-1]
ENST00000630618; ENSP00000486064; ENSG00000280477 [Q14644-1]
GeneIDi22821
KEGGihsa:22821
UCSCiuc001vui.4 human [Q14644-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRASA3_HUMAN
AccessioniPrimary (citable) accession number: Q14644
Secondary accession number(s): A6NL15, F8W6X8, Q8IUY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: September 23, 2008
Last modified: April 25, 2018
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health