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Q14644

- RASA3_HUMAN

UniProt

Q14644 - RASA3_HUMAN

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Protein

Ras GTPase-activating protein 3

Gene

RASA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri679 – 71537Btk-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-release channel activity Source: BHF-UCL
  2. GTPase activator activity Source: ProtInc
  3. metal ion binding Source: UniProtKB-KW
  4. Ras GTPase activator activity Source: RefGenome

GO - Biological processi

  1. calcium ion transmembrane transport Source: GOC
  2. intracellular signal transduction Source: InterPro
  3. negative regulation of Ras protein signal transduction Source: RefGenome
  4. positive regulation of Ras GTPase activity Source: RefGenome
  5. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 3
Alternative name(s):
GAP1(IP4BP)
Ins P4-binding protein
Gene namesi
Name:RASA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 13

Organism-specific databases

HGNCiHGNC:20331. RASA3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. intrinsic component of the cytoplasmic side of the plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi599 – 5991K → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication
Mutagenesisi600 – 6001K → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication
Mutagenesisi601 – 6011R → Q: No binding to IP4 and loss of plasma membrane localization. 1 Publication

Organism-specific databases

PharmGKBiPA134905447.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834Ras GTPase-activating protein 3PRO_0000056642Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphotyrosineBy similarity
Modified residuei110 – 1101Phosphothreonine1 Publication
Modified residuei809 – 8091Phosphoserine1 Publication
Modified residuei833 – 8331PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ14644.
PaxDbiQ14644.
PRIDEiQ14644.

PTM databases

PhosphoSiteiQ14644.

Expressioni

Gene expression databases

BgeeiQ14644.
CleanExiHS_RASA3.
ExpressionAtlasiQ14644. baseline and differential.
GenevestigatoriQ14644.

Organism-specific databases

HPAiHPA046759.

Interactioni

Protein-protein interaction databases

BioGridi116497. 7 interactions.
STRINGi9606.ENSP00000335029.

Structurei

3D structure databases

ProteinModelPortaliQ14644.
SMRiQ14644. Positions 11-275, 300-568, 577-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9696C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini132 – 247116C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini330 – 524195Ras-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini576 – 677102PHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri679 – 71537Btk-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG323905.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ14644.
KOiK12380.
OMAiPICDIPL.
OrthoDBiEOG7J17Z6.
PhylomeDBiQ14644.
TreeFamiTF105302.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
PRINTSiPR00402. TECBTKDOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q14644-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT
60 70 80 90 100
KIVEKSLCPF YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE
110 120 130 140 150
DLQKYHNRDT WFQLQHVDAD SEVQGKVHLE LRLSEVITDT GVVCHKLATR
160 170 180 190 200
IVECQGLPIV NGQCDPYATV TLAGPFRSEA KKTKVKRKTN NPQFDEVFYF
210 220 230 240 250
EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD EFLGELRIPL
260 270 280 290 300
KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY
310 320 330 340 350
SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP
360 370 380 390 400
FISAIASAEV KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI
410 420 430 440 450
EEICQSHKPC EIDPVKLKDG ENLENNMENL RQYVDRVFHA ITESGVSCPT
460 470 480 490 500
VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS FIFLRFFAPA ILSPNLFQLT
510 520 530 540 550
PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT FYEFFNEQKY
560 570 580 590 600
ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK
610 620 630 640 650
RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ
660 670 680 690 700
PERALYIQAN NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR
710 720 730 740 750
APSDSAPGCS PCTGGLPANI QLDIDGDRET ERIYSLFNLY MSKLEKMQEA
760 770 780 790 800
CGSKSVYDGP EQEEYSTFVI DDPQETYKTL KQVIAGVGAL EQEHAQYKRD
810 820 830
KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI
Length:834
Mass (Da):95,699
Last modified:September 23, 2008 - v3
Checksum:i97C3467C957DA8A1
GO
Isoform 2 (identifier: Q14644-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: No experimental confirmation available.

Show »
Length:802
Mass (Da):92,228
Checksum:iA694D90C28A1F18D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti785 – 7862AG → RW in CAA61580. (PubMed:7637787)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3232Missing in isoform 2. 1 PublicationVSP_056141Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89399 mRNA. Translation: CAA61580.1.
AK289746 mRNA. Translation: BAF82435.1.
AK294913 mRNA. Translation: BAH11922.1.
AL161774, BX537329 Genomic DNA. Translation: CAI12864.2.
BX537329, AL161774 Genomic DNA. Translation: CAM28365.1.
BC038456 mRNA. Translation: AAH38456.1.
BC047242 mRNA. Translation: AAH47242.1.
CCDSiCCDS32016.1. [Q14644-1]
RefSeqiNP_031394.2. NM_007368.2. [Q14644-1]
UniGeneiHs.593075.

Genome annotation databases

EnsembliENST00000334062; ENSP00000335029; ENSG00000185989. [Q14644-1]
GeneIDi22821.
KEGGihsa:22821.
UCSCiuc001vui.3. human. [Q14644-1]

Polymorphism databases

DMDMi206729910.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89399 mRNA. Translation: CAA61580.1 .
AK289746 mRNA. Translation: BAF82435.1 .
AK294913 mRNA. Translation: BAH11922.1 .
AL161774 , BX537329 Genomic DNA. Translation: CAI12864.2 .
BX537329 , AL161774 Genomic DNA. Translation: CAM28365.1 .
BC038456 mRNA. Translation: AAH38456.1 .
BC047242 mRNA. Translation: AAH47242.1 .
CCDSi CCDS32016.1. [Q14644-1 ]
RefSeqi NP_031394.2. NM_007368.2. [Q14644-1 ]
UniGenei Hs.593075.

3D structure databases

ProteinModelPortali Q14644.
SMRi Q14644. Positions 11-275, 300-568, 577-709.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116497. 7 interactions.
STRINGi 9606.ENSP00000335029.

PTM databases

PhosphoSitei Q14644.

Polymorphism databases

DMDMi 206729910.

Proteomic databases

MaxQBi Q14644.
PaxDbi Q14644.
PRIDEi Q14644.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000334062 ; ENSP00000335029 ; ENSG00000185989 . [Q14644-1 ]
GeneIDi 22821.
KEGGi hsa:22821.
UCSCi uc001vui.3. human. [Q14644-1 ]

Organism-specific databases

CTDi 22821.
GeneCardsi GC13M114747.
HGNCi HGNC:20331. RASA3.
HPAi HPA046759.
MIMi 605182. gene.
neXtProti NX_Q14644.
PharmGKBi PA134905447.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG323905.
GeneTreei ENSGT00760000119092.
HOGENOMi HOG000007183.
HOVERGENi HBG055643.
InParanoidi Q14644.
KOi K12380.
OMAi PICDIPL.
OrthoDBi EOG7J17Z6.
PhylomeDBi Q14644.
TreeFami TF105302.

Miscellaneous databases

ChiTaRSi RASA3. human.
GeneWikii RASA3.
GenomeRNAii 22821.
NextBioi 35512738.
PROi Q14644.
SOURCEi Search...

Gene expression databases

Bgeei Q14644.
CleanExi HS_RASA3.
ExpressionAtlasi Q14644. baseline and differential.
Genevestigatori Q14644.

Family and domain databases

Gene3Di 1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProi IPR000008. C2_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view ]
Pfami PF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view ]
PRINTSi PR00402. TECBTKDOMAIN.
SMARTi SM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
PROSITEi PS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a specific Ins(1,3,4,5)P4-binding protein as a member of the GAP1 family."
    Cullen P.J., Hsuan J.J., Truong O., Letcher A.J., Jackson T.J., Dawson A.P., Irvine R.F.
    Nature 376:527-530(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Peripheral blood.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  3. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Testis.
  5. "Distinct subcellular localisations of the putative inositol 1,3,4,5-tetrakisphosphate receptors GAP1(IP4BP) and GAP1m result from the GAP1(IP4BP) PH domain directing plasma membrane targeting."
    Lockyer P.J., Bottomley J.R., Reynolds J.S., McNulty T.J., Venkateswarlu K., Potter B.V.L., Dempsey C.E., Cullen P.J.
    Curr. Biol. 7:1007-1010(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
    Tissue: Blood.
  6. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-110, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRASA3_HUMAN
AccessioniPrimary (citable) accession number: Q14644
Secondary accession number(s): A6NL15, F8W6X8, Q8IUY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: September 23, 2008
Last modified: November 26, 2014
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3