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Q14624

- ITIH4_HUMAN

UniProt

Q14624 - ITIH4_HUMAN

Protein

Inter-alpha-trypsin inhibitor heavy chain H4

Gene

ITIH4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 4 (05 May 2009)
      Previous versions | rss
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    Functioni

    Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei688 – 6892Cleavage; by kallikrein

    GO - Molecular functioni

    1. endopeptidase inhibitor activity Source: ProtInc
    2. protein binding Source: UniProtKB
    3. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. acute-phase response Source: UniProtKB
    2. hyaluronan metabolic process Source: InterPro
    3. negative regulation of endopeptidase activity Source: GOC
    4. response to cytokine Source: UniProtKB

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Keywords - Biological processi

    Acute phase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inter-alpha-trypsin inhibitor heavy chain H4
    Short name:
    ITI heavy chain H4
    Short name:
    ITI-HC4
    Short name:
    Inter-alpha-inhibitor heavy chain 4
    Alternative name(s):
    Inter-alpha-trypsin inhibitor family heavy chain-related protein
    Short name:
    IHRP
    Plasma kallikrein sensitive glycoprotein 120
    Short name:
    Gp120
    Short name:
    PK-120
    Cleaved into the following 2 chains:
    Gene namesi
    Name:ITIH4
    Synonyms:IHRP, ITIHL1, PK120
    ORF Names:PRO1851
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:6169. ITIH4.

    Subcellular locationi

    GO - Cellular componenti

    1. blood microparticle Source: UniProt
    2. cytoplasm Source: Ensembl
    3. extracellular region Source: UniProtKB
    4. extracellular vesicular exosome Source: UniProt
    5. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29967.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 66163370 kDa inter-alpha-trypsin inhibitor heavy chain H4PRO_0000016541Add
    BLAST
    Propeptidei662 – 68827Potentially active peptidePRO_0000016542Add
    BLAST
    Chaini689 – 93024235 kDa inter-alpha-trypsin inhibitor heavy chain H4PRO_0000016543Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi81 – 811N-linked (GlcNAc...)3 Publications
    Glycosylationi207 – 2071N-linked (GlcNAc...)4 Publications
    Glycosylationi517 – 5171N-linked (GlcNAc...)4 Publications
    Glycosylationi577 – 5771N-linked (GlcNAc...)2 Publications
    Glycosylationi720 – 7201O-linked (GalNAc...)2 Publications
    Disulfide bondi747 ↔ 925Curated

    Post-translational modificationi

    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    N- and O-glycosylated. In urine, O-linked glycosylation on threonine residues in the region from Thr-719 to Thr-725 consists of core 1 or possibly core 8 glycans. Mainly Hex(HexNAc)2, but also some Hex3(HexNAc)3. N-glycosylated but not O-glycosylated in plasma.6 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ14624.
    PaxDbiQ14624.
    PRIDEiQ14624.

    PTM databases

    PhosphoSiteiQ14624.

    Expressioni

    Tissue specificityi

    Liver specific.2 Publications

    Inductioni

    Levels increase from 1.4 to 3-fold in acute-phase processes such as in acute ischemia stroke (AIS), unstable angina and programmed surgery. In hepatocytes, induced by IL6 but not by other cytokines such as IL1B.2 Publications

    Gene expression databases

    ArrayExpressiQ14624.
    BgeeiQ14624.
    CleanExiHS_ITIH4.
    GenevestigatoriQ14624.

    Organism-specific databases

    HPAiCAB016676.
    HPA001835.
    HPA003948.

    Interactioni

    Subunit structurei

    Interacts (via C-terminus) with DNAJC1 (via SANT 2 domain); this interaction protects ITIH4 against cleavage by kallikrein in vitro.1 Publication

    Protein-protein interaction databases

    BioGridi109906. 3 interactions.
    IntActiQ14624. 2 interactions.
    STRINGi9606.ENSP00000266041.

    Structurei

    3D structure databases

    ProteinModelPortaliQ14624.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 148120VITPROSITE-ProRule annotationAdd
    BLAST
    Domaini272 – 432161VWFAPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni658 – 68831Proline-rich (PRR) potential bioactive peptideAdd
    BLAST
    Regioni719 – 7257O-glycosylated at three sites

    Sequence similaritiesi

    Belongs to the ITIH family.Curated
    Contains 1 VIT domain.PROSITE-ProRule annotation
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2304.
    HOGENOMiHOG000239381.
    HOVERGENiHBG057734.
    InParanoidiQ14624.
    OrthoDBiEOG7CRTP5.
    PhylomeDBiQ14624.
    TreeFamiTF328982.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR010600. ITI_HC_C.
    IPR013694. VIT.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF06668. ITI_HC_C. 1 hit.
    PF08487. VIT. 1 hit.
    PF00092. VWA. 1 hit.
    [Graphical view]
    SMARTiSM00609. VIT. 1 hit.
    SM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    PROSITEiPS51468. VIT. 1 hit.
    PS50234. VWFA. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q14624-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKPPRPVRTC SKVLVLLSLL AIHQTTTAEK NGIDIYSLTV DSRVSSRFAH    50
    TVVTSRVVNR ANTVQEATFQ MELPKKAFIT NFSMIIDGMT YPGIIKEKAE 100
    AQAQYSAAVA KGKSAGLVKA TGRNMEQFQV SVSVAPNAKI TFELVYEELL 150
    KRRLGVYELL LKVRPQQLVK HLQMDIHIFE PQGISFLETE STFMTNQLVD 200
    ALTTWQNKTK AHIRFKPTLS QQQKSPEQQE TVLDGNLIIR YDVDRAISGG 250
    SIQIENGYFV HYFAPEGLTT MPKNVVFVID KSGSMSGRKI QQTREALIKI 300
    LDDLSPRDQF NLIVFSTEAT QWRPSLVPAS AENVNKARSF AAGIQALGGT 350
    NINDAMLMAV QLLDSSNQEE RLPEGSVSLI ILLTDGDPTV GETNPRSIQN 400
    NVREAVSGRY SLFCLGFGFD VSYAFLEKLA LDNGGLARRI HEDSDSALQL 450
    QDFYQEVANP LLTAVTFEYP SNAVEEVTQN NFRLLFKGSE MVVAGKLQDR 500
    GPDVLTATVS GKLPTQNITF QTESSVAEQE AEFQSPKYIF HNFMERLWAY 550
    LTIQQLLEQT VSASDADQQA LRNQALNLSL AYSFVTPLTS MVVTKPDDQE 600
    QSQVAEKPME GESRNRNVHS GSTFFKYYLQ GAKIPKPEAS FSPRRGWNRQ 650
    AGAAGSRMNF RPGVLSSRQL GLPGPPDVPD HAAYHPFRRL AILPASAPPA 700
    TSNPDPAVSR VMNMKIEETT MTTQTPAPIQ APSAILPLPG QSVERLCVDP 750
    RHRQGPVNLL SDPEQGVEVT GQYEREKAGF SWIEVTFKNP LVWVHASPEH 800
    VVVTRNRRSS AYKWKETLFS VMPGLKMTMD KTGLLLLSDP DKVTIGLLFW 850
    DGRGEGLRLL LRDTDRFSSH VGGTLGQFYQ EVLWGSPAAS DDGRRTLRVQ 900
    GNDHSATRER RLDYQEGPPG VEISCWSVEL 930
    Length:930
    Mass (Da):103,357
    Last modified:May 5, 2009 - v4
    Checksum:i847A9A5CB886C5A4
    GO
    Isoform 2 (identifier: Q14624-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         621-650: Missing.
         727-727: A → ACPSCSRSRAPAVPA

    Note: No experimental confirmation available.

    Show »
    Length:914
    Mass (Da):101,241
    Checksum:i79F2D903E65026CD
    GO
    Isoform 3 (identifier: Q14624-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         621-650: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:900
    Mass (Da):99,857
    Checksum:iB18BE8E1C3BF634C
    GO
    Isoform 4 (identifier: Q14624-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         621-650: Missing.
         727-765: Missing.
         851-866: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:845
    Mass (Da):93,852
    Checksum:i60436CD8050B7D46
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851I → K in AAD05198. (PubMed:8797089)Curated
    Sequence conflicti114 – 1141S → N in BAA07602. (PubMed:7775381)Curated
    Sequence conflicti207 – 2071N → F AA sequence (PubMed:7541790)Curated
    Sequence conflicti221 – 2211Q → E AA sequence (PubMed:7541790)Curated
    Sequence conflicti307 – 3071R → V AA sequence (PubMed:7541790)Curated
    Sequence conflicti322 – 3221W → Y AA sequence (PubMed:7541790)Curated
    Sequence conflicti370 – 3701E → G in BAH12781. (PubMed:16641997)Curated
    Sequence conflicti816 – 8172ET → QR AA sequence (PubMed:7541790)Curated
    Sequence conflicti905 – 9051S → F no nucleotide entry (PubMed:8797089)Curated
    Sequence conflicti927 – 9271S → T no nucleotide entry (PubMed:8797089)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti85 – 851I → N.1 Publication
    Corresponds to variant rs13072536 [ dbSNP | Ensembl ].
    VAR_027869
    Natural varianti86 – 861I → N.1 Publication
    VAR_013836
    Natural varianti669 – 6691Q → L.
    Corresponds to variant rs2276814 [ dbSNP | Ensembl ].
    VAR_027870
    Natural varianti698 – 6981P → T.
    Corresponds to variant rs4687657 [ dbSNP | Ensembl ].
    VAR_027871
    Natural varianti714 – 7141M → I.
    Corresponds to variant rs2256734 [ dbSNP | Ensembl ].
    VAR_027872
    Natural varianti791 – 7911L → P.
    Corresponds to variant rs2535621 [ dbSNP | Ensembl ].
    VAR_027873

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei621 – 65030Missing in isoform 2, isoform 3 and isoform 4. 3 PublicationsVSP_002761Add
    BLAST
    Alternative sequencei727 – 76539Missing in isoform 4. 1 PublicationVSP_044764Add
    BLAST
    Alternative sequencei727 – 7271A → ACPSCSRSRAPAVPA in isoform 2. 1 PublicationVSP_002762
    Alternative sequencei851 – 86616Missing in isoform 4. 1 PublicationVSP_044765Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38535 mRNA. Translation: BAA07536.1.
    D38595 mRNA. Translation: BAA07602.1.
    U43163
    , U42015, U42016, U43155, U43156, U43157, U43158, U43159, U43160, U43161, U43162 Genomic DNA. Translation: AAD05198.1.
    AK298412 mRNA. Translation: BAH12781.1.
    AC006254 Genomic DNA. No translation available.
    AC099667 Genomic DNA. No translation available.
    AF119856 mRNA. Translation: AAF69610.1.
    CCDSiCCDS2865.1. [Q14624-1]
    CCDS54596.1. [Q14624-3]
    PIRiJX0368.
    RefSeqiNP_001159921.1. NM_001166449.1. [Q14624-3]
    NP_002209.2. NM_002218.4. [Q14624-1]
    UniGeneiHs.709406.

    Genome annotation databases

    EnsembliENST00000266041; ENSP00000266041; ENSG00000055955. [Q14624-1]
    ENST00000406595; ENSP00000384425; ENSG00000055955. [Q14624-3]
    GeneIDi3700.
    KEGGihsa:3700.
    UCSCiuc003dfz.3. human. [Q14624-1]
    uc011bel.2. human. [Q14624-2]

    Polymorphism databases

    DMDMi229463048.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38535 mRNA. Translation: BAA07536.1 .
    D38595 mRNA. Translation: BAA07602.1 .
    U43163
    , U42015 , U42016 , U43155 , U43156 , U43157 , U43158 , U43159 , U43160 , U43161 , U43162 Genomic DNA. Translation: AAD05198.1 .
    AK298412 mRNA. Translation: BAH12781.1 .
    AC006254 Genomic DNA. No translation available.
    AC099667 Genomic DNA. No translation available.
    AF119856 mRNA. Translation: AAF69610.1 .
    CCDSi CCDS2865.1. [Q14624-1 ]
    CCDS54596.1. [Q14624-3 ]
    PIRi JX0368.
    RefSeqi NP_001159921.1. NM_001166449.1. [Q14624-3 ]
    NP_002209.2. NM_002218.4. [Q14624-1 ]
    UniGenei Hs.709406.

    3D structure databases

    ProteinModelPortali Q14624.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109906. 3 interactions.
    IntActi Q14624. 2 interactions.
    STRINGi 9606.ENSP00000266041.

    PTM databases

    PhosphoSitei Q14624.

    Polymorphism databases

    DMDMi 229463048.

    Proteomic databases

    MaxQBi Q14624.
    PaxDbi Q14624.
    PRIDEi Q14624.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000266041 ; ENSP00000266041 ; ENSG00000055955 . [Q14624-1 ]
    ENST00000406595 ; ENSP00000384425 ; ENSG00000055955 . [Q14624-3 ]
    GeneIDi 3700.
    KEGGi hsa:3700.
    UCSCi uc003dfz.3. human. [Q14624-1 ]
    uc011bel.2. human. [Q14624-2 ]

    Organism-specific databases

    CTDi 3700.
    GeneCardsi GC03M052846.
    HGNCi HGNC:6169. ITIH4.
    HPAi CAB016676.
    HPA001835.
    HPA003948.
    MIMi 600564. gene.
    neXtProti NX_Q14624.
    PharmGKBi PA29967.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2304.
    HOGENOMi HOG000239381.
    HOVERGENi HBG057734.
    InParanoidi Q14624.
    OrthoDBi EOG7CRTP5.
    PhylomeDBi Q14624.
    TreeFami TF328982.

    Miscellaneous databases

    ChiTaRSi ITIH4. human.
    GeneWikii ITIH4.
    GenomeRNAii 3700.
    NextBioi 14503.
    PROi Q14624.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14624.
    Bgeei Q14624.
    CleanExi HS_ITIH4.
    Genevestigatori Q14624.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR010600. ITI_HC_C.
    IPR013694. VIT.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF06668. ITI_HC_C. 1 hit.
    PF08487. VIT. 1 hit.
    PF00092. VWA. 1 hit.
    [Graphical view ]
    SMARTi SM00609. VIT. 1 hit.
    SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    PROSITEi PS51468. VIT. 1 hit.
    PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA and deduced amino acid sequence of human PK-120, a plasma kallikrein-sensitive glycoprotein."
      Nishimura H., Kakizaki I., Muta T., Sasaki N., Pu P.X., Yamashita T., Nagasawa S.
      FEBS Lett. 357:207-211(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "Cloning and characterization of cDNA for inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP), a novel human plasma glycoprotein."
      Saguchi K., Tobe T., Hashimoto K., Sano Y., Nakano Y., Miura N.-H., Tomita M.
      J. Biochem. 117:14-18(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, IDENTIFICATION, TISSUE SPECIFICITY, VARIANT ASN-85.
      Tissue: Liver.
    3. "Isolation and characterization of the human inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) gene (ITIHL1)."
      Saguchi K., Tobe T., Hashimoto K., Nagasaki Y., Oda E., Nakano Y., Miura N.H., Tomita M.
      J. Biochem. 119:898-905(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Liver.
    5. "BIP co-chaperone MTJ1/ERDJ1 interacts with inter-alpha-trypsin inhibitor heavy chain 4."
      Kroczynska B., King-Simmons L., Alloza L., Alava M.A., Elguindi E.C., Blond S.Y.
      Biochem. Biophys. Res. Commun. 338:1467-1477(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), INTERACTION WITH DNAJC1.
    6. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "Functional prediction of the coding sequences of 79 new genes deduced by analysis of cDNA clones from human fetal liver."
      Zhang C., Yu Y., Zhang S., Wei H., Zhang Y., Zhou G., Bi J., Liu M., He F.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 271-930 (ISOFORM 2).
      Tissue: Fetal liver.
    8. "Purification and characterization of a novel glycoprotein which has significant homology to heavy chains of inter-alpha-trypsin inhibitor family from human plasma."
      Choi-Miura N.-H., Sano Y., Oda E., Nakano Y., Tobe T., Yanagishita T., Taniyama M., Katagiri T., Tomita M.
      J. Biochem. 117:400-407(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-44; 99-119; 140-151; 163-170; 194-208; 211-243; 274-281; 290-329; 429-487; 497-567; 608-626 AND 816-831, PROTEOLYTIC PROCESSING, GLYCOSYLATION.
      Tissue: Plasma.
    9. "Purification and characterization of a novel substrate for plasma kallikrein (PK-120) in human plasma."
      Pu X.P., Iwamoto A., Nishimura H., Nagasawa S.
      Biochim. Biophys. Acta 1208:338-343(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, PROTEOLYTIC PROCESSING.
    10. "ITIH4 serum concentration increases during acute-phase processes in human patients and is up-regulated by interleukin-6 in hepatocarcinoma HepG2 cells."
      Pineiro M., Alava M.A., Gonzalez-Ramon N., Osada J., Lasierra P., Larrad L., Pineiro A., Lampreave F.
      Biochem. Biophys. Res. Commun. 263:224-229(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, INDUCTION.
    11. "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
      Zhang H., Li X.-J., Martin D.B., Aebersold R.
      Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-207.
    12. "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
      Bunkenborg J., Pilch B.J., Podtelejnikov A.V., Wisniewski J.R.
      Proteomics 4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-517.
      Tissue: Plasma.
    13. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-207; ASN-517 AND ASN-577.
      Tissue: Plasma.
    14. "Inter-alpha-trypsin inhibitor heavy chain 4 is a novel marker of acute ischemic stroke."
      Kashyap R.S., Nayak A.R., Deshpande P.S., Kabra D., Purohit H.J., Taori G.M., Daginawala H.F.
      Clin. Chim. Acta 402:160-163(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    15. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-81; ASN-207 AND ASN-517.
      Tissue: Liver.
    16. "The absolute quantification of eight inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4)-derived peptides in serum from breast cancer patients."
      van den Broek I., Sparidans R.W., van Winden A.W., Gast M.C., van Dulken E.J., Schellens J.H., Beijnen J.H.
      Proteomics Clin. Appl. 4:931-939(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE ROLE OF ACTIVE PEPTIDE AS A BIOMARKER FOR BREAST CANCER.
    17. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
      Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G.
      Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT THR-720, STRUCTURE OF CARBOHYDRATES, IDENTIFICATION BY MASS SPECTROMETRY.
    19. "SNP analysis of the inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) gene by a fluorescence-adapted SSCP method."
      Tozaki T., Choi-Miura N.-H., Taniyama M., Kurosawa M., Tomita M.
      BMC Med. Genet. 3:6-6(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT ASN-86.

    Entry informationi

    Entry nameiITIH4_HUMAN
    AccessioniPrimary (citable) accession number: Q14624
    Secondary accession number(s): B7Z545
    , E9PGN5, Q15135, Q9P190, Q9UQ54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 136 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Possible biomarker for acute ischemic stroke (PubMed:19263524). Peptides derived from the proline-rich potentially active peptide (PRO_0000016542) may be biomarkers for a variety of disease states including breast cancer (PubMed:21137033).2 Publications

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3