Q145F5 (HEMH_BURXL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	Bxeno_A0496
ORF Names:	Bxe_A3965

Organism

Burkholderia xenovorans (strain LB400) [Complete proteome] [HAMAP]

Taxonomic identifier

266265 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Ferrochelatase HAMAP MF_00323		PRO_1000059477
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q145F5 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 66345BF35244923A	FASTA	356	39,782
	10 20 30 40 50 60 MRFDLERPSQ NATSHRVAVL LINLGTPDAP TPRAVRRYLA QFLSDPRVVE IPALLWQIIL 70 80 90 100 110 120 RLLILPFRGS ASAKKYAAVW MPEGSPLRVY TEKQVEGLRH LLQLNDYTVL VDYAMRYGTP 130 140 150 160 170 180 GIPAMLNQLK LAGAERVLLM PMYPQYSSST TATAFDDAFS ALKRMRNQPE IRTVRQYADH 190 200 210 220 230 240 PAYIAALAAQ VHQYWHQHGR PDFAAGDKLV LSFHGVPKRT LDLGDPYHEQ CQQTGALLMQ 250 260 270 280 290 300 ALELTPVECR ITFQSRFGKA EWLQPYTAPT LKELGAAGVR RADVFCPGFT ADCLETIEEI 310 320 330 340 350 GMEVRDEFLH AGGKDFHRIP CLNASQAWIA ALGEIVAQNL QGWPVQALPV PHTTGA

References

[1]

"Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility." Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V., Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J., Parnell J.J. , Ramette A., Richardson P., Seeger M., Smith D., Spilker T., Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M. Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006) [PubMed: 17030797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LB400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000270 Genomic DNA. Translation: ABE29034.1.
RefSeq	YP_557086.1. NC_007951.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q145F5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4004628.
GenomeReviews	Gene locus Bxeno_A0496 in contig CP000270_GR.
KEGG	bxe:Bxe_A3965.
PATRIC	19326373. VBIBurXen52548_0527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697135.
OMA	TIEEIGM.
PhylomeDB	Q145F5.
ProtClustDB	PRK00035.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURXL

Accession

Primary (citable) accession number: Q145F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program