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Protein

Next to BRCA1 gene 1 protein

Gene

NBR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri211 – 257ZZ-typePROSITE-ProRule annotationAdd BLAST47

GO - Molecular functioni

  • mitogen-activated protein kinase binding Source: BHF-UCL
  • ubiquitin binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-33579-MONOMER.
SIGNORiQ14596.

Names & Taxonomyi

Protein namesi
Recommended name:
Next to BRCA1 gene 1 protein
Alternative name(s):
Cell migration-inducing gene 19 protein
Membrane component chromosome 17 surface marker 2
Neighbor of BRCA1 gene 1 protein
Protein 1A1-3B
Gene namesi
Name:NBR1
Synonyms:1A13B, KIAA0049, M17S2
ORF Names:MIG19
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:6746. NBR1.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: GO_Central
  • cytoplasmic vesicle Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • late endosome Source: UniProtKB
  • lysosome Source: UniProtKB-SubCell
  • M band Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • pre-autophagosomal structure Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Lysosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12K → A: No effect on interaction with SQSTM1. 1 Publication1
Mutagenesisi50D → R: Loss of interaction with SQSTM1. 2 Publications1
Mutagenesisi732Y → A: Loss of interaction ATG8 family proteins. 1 Publication1

Organism-specific databases

DisGeNETi4077.
OpenTargetsiENSG00000188554.
PharmGKBiPA30510.

Polymorphism and mutation databases

BioMutaiNBR1.
DMDMi296439290.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967461 – 966Next to BRCA1 gene 1 proteinAdd BLAST966

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116PhosphoserineCombined sources1
Modified residuei590PhosphoserineBy similarity1
Modified residuei596PhosphoserineBy similarity1
Modified residuei625PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14596.
MaxQBiQ14596.
PaxDbiQ14596.
PeptideAtlasiQ14596.
PRIDEiQ14596.

PTM databases

iPTMnetiQ14596.
PhosphoSitePlusiQ14596.
UniCarbKBiQ14596.

Expressioni

Gene expression databases

BgeeiENSG00000188554.
CleanExiHS_NBR1.
ExpressionAtlasiQ14596. baseline and differential.
GenevisibleiQ14596. HS.

Organism-specific databases

HPAiHPA022999.
HPA023999.

Interactioni

Subunit structurei

Homooligomer and heterooligomer. Interacts with TRIM55 (By similarity). Interacts with SQSTM1, titin/TTN, RNF29, USP8, SQSTM1, MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2. Binds to ubiquitin and ubiquitinated proteins.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG8P381827EBI-742698,EBI-2684From a different organism.
GABARAPO951665EBI-742698,EBI-712001
GABARAPL1Q9H0R84EBI-742698,EBI-746969
GABARAPL2P6052011EBI-742698,EBI-720116
HTTP428583EBI-742698,EBI-466029
MAP1LC3AQ9H4925EBI-742698,EBI-720768
MAP1LC3BQ9GZQ88EBI-742698,EBI-373144
MAP1LC3CQ9BXW43EBI-742698,EBI-2603996
SQSTM1Q135016EBI-742698,EBI-307104
SYT4Q9H2B23EBI-742698,EBI-751132
USP8P408183EBI-742698,EBI-1050865

GO - Molecular functioni

  • mitogen-activated protein kinase binding Source: BHF-UCL
  • ubiquitin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110253. 42 interactors.
IntActiQ14596. 27 interactors.
MINTiMINT-2806845.
STRINGi9606.ENSP00000343479.

Structurei

Secondary structure

1966
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 11Combined sources7
Beta strandi14 – 21Combined sources8
Helixi23 – 25Combined sources3
Helixi28 – 39Combined sources12
Beta strandi42 – 49Combined sources8
Beta strandi55 – 58Combined sources4
Helixi61 – 73Combined sources13
Turni74 – 76Combined sources3
Beta strandi77 – 84Combined sources8
Beta strandi373 – 380Combined sources8
Beta strandi384 – 386Combined sources3
Beta strandi391 – 400Combined sources10
Beta strandi402 – 404Combined sources3
Beta strandi410 – 418Combined sources9
Beta strandi420 – 423Combined sources4
Helixi425 – 428Combined sources4
Beta strandi439 – 447Combined sources9
Beta strandi453 – 463Combined sources11
Beta strandi466 – 478Combined sources13
Helixi917 – 927Combined sources11
Helixi932 – 939Combined sources8
Turni940 – 944Combined sources5
Helixi946 – 956Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJ6NMR-A1-85[»]
2BKFX-ray1.56A1-85[»]
2CP8NMR-A916-956[»]
2G4SX-ray2.15A1-85[»]
2L8JNMR-B726-738[»]
2MGWNMR-A913-959[»]
2MJ5NMR-B913-959[»]
4OLEX-ray2.52A/B/C/D365-485[»]
ProteinModelPortaliQ14596.
SMRiQ14596.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14596.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 85PB1PROSITE-ProRule annotationAdd BLAST82
Domaini913 – 957UBAPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni542 – 636ATG8 family protein-bindingAdd BLAST95
Regioni727 – 738ATG8 family protein-bindingAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi703 – 714Poly-GluAdd BLAST12

Domaini

The PB1 domain mediates interaction with SQSTM1.2 Publications

Sequence similaritiesi

Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation
Contains 1 ZZ-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri211 – 257ZZ-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4351. Eukaryota.
KOG4582. Eukaryota.
ENOG4111TKN. LUCA.
GeneTreeiENSGT00390000016335.
HOGENOMiHOG000220861.
HOVERGENiHBG052578.
InParanoidiQ14596.
KOiK17987.
OMAiAHLFEMG.
OrthoDBiEOG091G0RGJ.
PhylomeDBiQ14596.
TreeFamiTF328428.

Family and domain databases

CDDicd14947. NBR1_like. 1 hit.
InterProiIPR032350. N_BRCA1_central.
IPR033513. NBR1.
IPR000270. PB1_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR000433. Znf_ZZ.
[Graphical view]
PANTHERiPTHR20930:SF2. PTHR20930:SF2. 3 hits.
PfamiPF16158. N_BRCA1_IG. 1 hit.
PF00564. PB1. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50030. UBA. 1 hit.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14596-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPQVTLNVT FKNEIQSFLV SDPENTTWAD IEAMVKVSFD LNTIQIKYLD
60 70 80 90 100
EENEEVSINS QGEYEEALKM AVKQGNQLQM QVHEGHHVVD EAPPPVVGAK
110 120 130 140 150
RLAARAGKKP LAHYSSLVRV LGSDMKTPED PAVQSFPLVP CDTDQPQDKP
160 170 180 190 200
PDWFTSYLET FREQVVNETV EKLEQKLHEK LVLQNPSLGS CPSEVSMPTS
210 220 230 240 250
EETLFLPENQ FSWHIACNNC QRRIVGVRYQ CSLCPSYNIC EDCEAGPYGH
260 270 280 290 300
DTNHVLLKLR RPVVGSSEPF CHSKYSTPRL PAALEQVRLQ KQVDKNFLKA
310 320 330 340 350
EKQRLRAEKK QRKAEVKELK KQLKLHRKIH LWNSIHGLQS PKSPLGRPES
360 370 380 390 400
LLQSNTLMLP LQPCTSVMPM LSAAFVDENL PDGTHLQPGT KFIKHWRMKN
410 420 430 440 450
TGNVKWSADT KLKFMWGNLT LASTEKKDVL VPCLKAGHVG VVSVEFIAPA
460 470 480 490 500
LEGTYTSHWR LSHKGQQFGP RVWCSIIVDP FPSEESPDNI EKGMISSSKT
510 520 530 540 550
DDLTCQQEET FLLAKEERQL GEVTEQTEGT AACIPQKAKN VASERELYIP
560 570 580 590 600
SVDLLTAQDL LSFELLDINI VQELERVPHN TPVDVTPCMS PLPHDSPLIE
610 620 630 640 650
KPGLGQIEEE NEGAGFKALP DSMVSVKRKA ENIASVEEAE EDLSGTQFVC
660 670 680 690 700
ETVIRSLTLD AAPDHNPPCR QKSLQMTFAL PEGPLGNEKE EIIHIAEEEA
710 720 730 740 750
VMEEEEDEED EEEEDELKDE VQSQSSASSE DYIIILPECF DTSRPLGDSM
760 770 780 790 800
YSSALSQPGL ERGAEGKPGV EAGQEPAEAG ERLPGGENQP QEHSISDILT
810 820 830 840 850
TSQTLETVPL IPEVVELPPS LPRSSPCVHH HGSPGVDLPV TIPEVSSVPD
860 870 880 890 900
QIRGEPRGSS GLVNSRQKSY DHSRHHHGSS IAGGLVKGAL SVAASAYKAL
910 920 930 940 950
FAGPPVTAQP IISEDQTAAL MAHLFEMGFC DRQLNLRLLK KHNYNILQVV
960
TELLQLNNND WYSQRY
Length:966
Mass (Da):107,413
Last modified:May 18, 2010 - v3
Checksum:i6A057E67947838EF
GO
Isoform 2 (identifier: Q14596-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     910-966: PIISEDQTAA...NNNDWYSQRY → GLWGLLSFLHLAKKCFFLKAPSEAFSWF

Note: No experimental confirmation available.
Show »
Length:937
Mass (Da):103,834
Checksum:i0228655991281A88
GO

Sequence cautioni

The sequence BAA06417 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti746 – 770LGDSM…GKPGV → WGILCTALRSHSQAWSEVLK ASLGF in CAA54274 (PubMed:8069304).CuratedAdd BLAST25

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_016106923H → R.3 PublicationsCorresponds to variant rs8482dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004314910 – 966PIISE…YSQRY → GLWGLLSFLHLAKKCFFLKA PSEAFSWF in isoform 2. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76952 mRNA. Translation: CAA54274.1.
D30756 mRNA. Translation: BAA06417.2. Different initiation.
AY450308 mRNA. Translation: AAS15047.1.
AC060780 Genomic DNA. No translation available.
AC109326 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60946.1.
BC009808 mRNA. Translation: AAH09808.1.
AF227189 mRNA. Translation: AAF74119.1.
U25764 Genomic DNA. Translation: AAA93228.1.
CCDSiCCDS45694.1. [Q14596-1]
CCDS77037.1. [Q14596-2]
RefSeqiNP_001278500.1. NM_001291571.1. [Q14596-2]
NP_001278501.1. NM_001291572.1.
NP_005890.2. NM_005899.4. [Q14596-1]
NP_114068.1. NM_031862.3. [Q14596-1]
XP_011523115.1. XM_011524813.1. [Q14596-1]
XP_016880131.1. XM_017024642.1. [Q14596-1]
UniGeneiHs.277721.
Hs.708158.

Genome annotation databases

EnsembliENST00000341165; ENSP00000343479; ENSG00000188554. [Q14596-1]
ENST00000589872; ENSP00000467816; ENSG00000188554. [Q14596-2]
ENST00000590996; ENSP00000466667; ENSG00000188554. [Q14596-1]
GeneIDi4077.
KEGGihsa:4077.
UCSCiuc010czd.4. human. [Q14596-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76952 mRNA. Translation: CAA54274.1.
D30756 mRNA. Translation: BAA06417.2. Different initiation.
AY450308 mRNA. Translation: AAS15047.1.
AC060780 Genomic DNA. No translation available.
AC109326 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60946.1.
BC009808 mRNA. Translation: AAH09808.1.
AF227189 mRNA. Translation: AAF74119.1.
U25764 Genomic DNA. Translation: AAA93228.1.
CCDSiCCDS45694.1. [Q14596-1]
CCDS77037.1. [Q14596-2]
RefSeqiNP_001278500.1. NM_001291571.1. [Q14596-2]
NP_001278501.1. NM_001291572.1.
NP_005890.2. NM_005899.4. [Q14596-1]
NP_114068.1. NM_031862.3. [Q14596-1]
XP_011523115.1. XM_011524813.1. [Q14596-1]
XP_016880131.1. XM_017024642.1. [Q14596-1]
UniGeneiHs.277721.
Hs.708158.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJ6NMR-A1-85[»]
2BKFX-ray1.56A1-85[»]
2CP8NMR-A916-956[»]
2G4SX-ray2.15A1-85[»]
2L8JNMR-B726-738[»]
2MGWNMR-A913-959[»]
2MJ5NMR-B913-959[»]
4OLEX-ray2.52A/B/C/D365-485[»]
ProteinModelPortaliQ14596.
SMRiQ14596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110253. 42 interactors.
IntActiQ14596. 27 interactors.
MINTiMINT-2806845.
STRINGi9606.ENSP00000343479.

PTM databases

iPTMnetiQ14596.
PhosphoSitePlusiQ14596.
UniCarbKBiQ14596.

Polymorphism and mutation databases

BioMutaiNBR1.
DMDMi296439290.

Proteomic databases

EPDiQ14596.
MaxQBiQ14596.
PaxDbiQ14596.
PeptideAtlasiQ14596.
PRIDEiQ14596.

Protocols and materials databases

DNASUi4077.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341165; ENSP00000343479; ENSG00000188554. [Q14596-1]
ENST00000589872; ENSP00000467816; ENSG00000188554. [Q14596-2]
ENST00000590996; ENSP00000466667; ENSG00000188554. [Q14596-1]
GeneIDi4077.
KEGGihsa:4077.
UCSCiuc010czd.4. human. [Q14596-1]

Organism-specific databases

CTDi4077.
DisGeNETi4077.
GeneCardsiNBR1.
HGNCiHGNC:6746. NBR1.
HPAiHPA022999.
HPA023999.
MIMi166945. gene.
neXtProtiNX_Q14596.
OpenTargetsiENSG00000188554.
PharmGKBiPA30510.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4351. Eukaryota.
KOG4582. Eukaryota.
ENOG4111TKN. LUCA.
GeneTreeiENSGT00390000016335.
HOGENOMiHOG000220861.
HOVERGENiHBG052578.
InParanoidiQ14596.
KOiK17987.
OMAiAHLFEMG.
OrthoDBiEOG091G0RGJ.
PhylomeDBiQ14596.
TreeFamiTF328428.

Enzyme and pathway databases

BioCyciZFISH:G66-33579-MONOMER.
SIGNORiQ14596.

Miscellaneous databases

EvolutionaryTraceiQ14596.
GeneWikiiNBR1.
GenomeRNAii4077.
PROiQ14596.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188554.
CleanExiHS_NBR1.
ExpressionAtlasiQ14596. baseline and differential.
GenevisibleiQ14596. HS.

Family and domain databases

CDDicd14947. NBR1_like. 1 hit.
InterProiIPR032350. N_BRCA1_central.
IPR033513. NBR1.
IPR000270. PB1_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR000433. Znf_ZZ.
[Graphical view]
PANTHERiPTHR20930:SF2. PTHR20930:SF2. 3 hits.
PfamiPF16158. N_BRCA1_IG. 1 hit.
PF00564. PB1. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50030. UBA. 1 hit.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNBR1_HUMAN
AccessioniPrimary (citable) accession number: Q14596
Secondary accession number(s): Q13173
, Q15026, Q5J7Q8, Q96GB6, Q9NRF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 170 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to be the ovarian carcinoma antigen CA125.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.