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Q14596 (NBR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Next to BRCA1 gene 1 protein
Alternative name(s):
Cell migration-inducing gene 19 protein
Membrane component chromosome 17 surface marker 2
Neighbor of BRCA1 gene 1 protein
Protein 1A1-3B
Gene names
Name:NBR1
Synonyms:1A13B, KIAA0049, M17S2
ORF Names:MIG19
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length966 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. Ref.12

Subunit structure

Homooligomer and heterooligomer. Interacts with TRIM55 By similarity. Interacts with SQSTM1, titin/TTN, RNF29, USP8, SQSTM1, MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2. Binds to ubiquitin and ubiquitinated proteins. Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location

Cytoplasm. Cytoplasmic vesicleautophagosome. Lysosome. CytoplasmmyofibrilsarcomereM line By similarity. Note: In cardiac muscles localizes to the sarcomeric M line By similarity. Is targeted to lysosomes for degradation. Ref.11 Ref.12

Domain

The OPR domain mediates interaction with SQSTM1. Ref.9 Ref.12

Sequence similarities

Contains 1 OPR domain.

Contains 1 UBA domain.

Contains 1 ZZ-type zinc finger.

Caution

Was originally (Ref.1) thought to be the ovarian carcinoma antigen CA125.

Sequence caution

The sequence BAA06417.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoplasmic vesicle
Lysosome
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmacroautophagy

Inferred from direct assay Ref.12. Source: UniProtKB

negative regulation of osteoblast differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

protein oligomerization

Inferred from direct assay Ref.12. Source: UniProtKB

regulation of bone mineralization

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of stress-activated MAPK cascade

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentM band

Inferred from electronic annotation. Source: UniProtKB-SubCell

autophagic vacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

cytosol

Inferred from direct assay Ref.12. Source: UniProtKB

late endosome

Inferred from sequence or structural similarity. Source: UniProtKB

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

pre-autophagosomal structure

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionmitogen-activated protein kinase binding

Inferred from sequence or structural similarity. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 19822672. Source: UniProtKB

ubiquitin binding

Inferred from direct assay Ref.11. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q14596-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q14596-2)

The sequence of this isoform differs from the canonical sequence as follows:
     910-966: PIISEDQTAA...NNNDWYSQRY → GLWGLLSFLHLAKKCFFLKAPSEAFSWF
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 966966Next to BRCA1 gene 1 protein
PRO_0000096746

Regions

Domain43 – 7129OPR
Domain913 – 95745UBA
Zinc finger211 – 25747ZZ-type
Region542 – 63695ATG8 family protein-binding
Region727 – 73812ATG8 family protein-binding
Compositional bias703 – 71412Poly-Glu

Natural variations

Alternative sequence910 – 96657PIISE…YSQRY → GLWGLLSFLHLAKKCFFLKA PSEAFSWF in isoform 2.
VSP_004314
Natural variant9231H → R. Ref.1 Ref.6 Ref.7
Corresponds to variant rs8482 [ dbSNP | Ensembl ].
VAR_016106

Experimental info

Mutagenesis121K → A: No effect on interaction with SQSTM1. Ref.9
Mutagenesis501D → R: Loss of interaction with SQSTM1. Ref.9 Ref.12
Mutagenesis7321Y → A: Loss of interaction ATG8 family proteins. Ref.12
Sequence conflict746 – 77025LGDSM…GKPGV → WGILCTALRSHSQAWSEVLK ASLGF in CAA54274. Ref.1

Secondary structure

............................................ 966
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 6A057E67947838EF

FASTA966107,413
        10         20         30         40         50         60 
MEPQVTLNVT FKNEIQSFLV SDPENTTWAD IEAMVKVSFD LNTIQIKYLD EENEEVSINS 

        70         80         90        100        110        120 
QGEYEEALKM AVKQGNQLQM QVHEGHHVVD EAPPPVVGAK RLAARAGKKP LAHYSSLVRV 

       130        140        150        160        170        180 
LGSDMKTPED PAVQSFPLVP CDTDQPQDKP PDWFTSYLET FREQVVNETV EKLEQKLHEK 

       190        200        210        220        230        240 
LVLQNPSLGS CPSEVSMPTS EETLFLPENQ FSWHIACNNC QRRIVGVRYQ CSLCPSYNIC 

       250        260        270        280        290        300 
EDCEAGPYGH DTNHVLLKLR RPVVGSSEPF CHSKYSTPRL PAALEQVRLQ KQVDKNFLKA 

       310        320        330        340        350        360 
EKQRLRAEKK QRKAEVKELK KQLKLHRKIH LWNSIHGLQS PKSPLGRPES LLQSNTLMLP 

       370        380        390        400        410        420 
LQPCTSVMPM LSAAFVDENL PDGTHLQPGT KFIKHWRMKN TGNVKWSADT KLKFMWGNLT 

       430        440        450        460        470        480 
LASTEKKDVL VPCLKAGHVG VVSVEFIAPA LEGTYTSHWR LSHKGQQFGP RVWCSIIVDP 

       490        500        510        520        530        540 
FPSEESPDNI EKGMISSSKT DDLTCQQEET FLLAKEERQL GEVTEQTEGT AACIPQKAKN 

       550        560        570        580        590        600 
VASERELYIP SVDLLTAQDL LSFELLDINI VQELERVPHN TPVDVTPCMS PLPHDSPLIE 

       610        620        630        640        650        660 
KPGLGQIEEE NEGAGFKALP DSMVSVKRKA ENIASVEEAE EDLSGTQFVC ETVIRSLTLD 

       670        680        690        700        710        720 
AAPDHNPPCR QKSLQMTFAL PEGPLGNEKE EIIHIAEEEA VMEEEEDEED EEEEDELKDE 

       730        740        750        760        770        780 
VQSQSSASSE DYIIILPECF DTSRPLGDSM YSSALSQPGL ERGAEGKPGV EAGQEPAEAG 

       790        800        810        820        830        840 
ERLPGGENQP QEHSISDILT TSQTLETVPL IPEVVELPPS LPRSSPCVHH HGSPGVDLPV 

       850        860        870        880        890        900 
TIPEVSSVPD QIRGEPRGSS GLVNSRQKSY DHSRHHHGSS IAGGLVKGAL SVAASAYKAL 

       910        920        930        940        950        960 
FAGPPVTAQP IISEDQTAAL MAHLFEMGFC DRQLNLRLLK KHNYNILQVV TELLQLNNND 


WYSQRY 

« Hide

Isoform 2 [UniParc].

Checksum: 0228655991281A88
Show »

FASTA937103,834

References

« Hide 'large scale' references
[1]"A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1."
Campbell I.G., Nicolai H.M., Foulkes W.D., Senger G., Stamp G.W., Allan G., Boyer C., Jones K., Bast R.C. Jr., Solomon E., Trowsdale J., Black D.M.
Hum. Mol. Genet. 3:589-594(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-923.
Tissue: Ovary.
[2]"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone marrow.
[3]"Identification of a human migration inducing gene."
Kim J.W.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ARG-923.
Tissue: Lymph.
[7]"Expression profiles and intergenic structure of head-to-head oriented Brca1 and Nbr1 genes."
Dimitrov S., Brennerova M., Forejt J.
Gene 262:89-98(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-42, VARIANT ARG-923.
Tissue: Testis.
[8]"Comparison of the positional cloning methods used to isolate the BRCA1 gene."
Harshman K., Bell R., Rosenthal J., Katcher H., Miki Y., Swenson J., Gholami Z., Frye C., Ding W., Dayananth P., Eddington K., Norris F.H., Bristow P.K., Phelps R., Hattier T., Stone S., Shaffer D., Bayer S. expand/collapse author list , Hussey C., Tran T., Lai M., Rosteck P.R. Jr., Skolnick M.H., Shattuck-Eidens D., Kamb A.
Hum. Mol. Genet. 4:1259-1266(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 412-508.
[9]"Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins."
Lamark T., Perander M., Outzen H., Kristiansen K., Oevervatn A., Michaelsen E., Bjoerkoey G., Johansen T.
J. Biol. Chem. 278:34568-34581(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SQSTM1, OLIGOMERIZATION, DOMAIN, MUTAGENESIS OF LYS-12 AND ASP-50.
[10]"The kinase domain of titin controls muscle gene expression and protein turnover."
Lange S., Xiang F., Yakovenko A., Vihola A., Hackman P., Rostkova E., Kristensen J., Brandmeier B., Franzen G., Hedberg B., Gunnarsson L.G., Hughes S.M., Marchand S., Sejersen T., Richard I., Edstroem L., Ehler E., Udd B., Gautel M.
Science 308:1599-1603(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SQSTM1; TTN AND RNF29.
[11]"Interactions with LC3 and polyubiquitin chains link nbr1 to autophagic protein turnover."
Waters S., Marchbank K., Solomon E., Whitehouse C., Gautel M.
FEBS Lett. 583:1846-1852(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH USP8, UBIQUITIN-BINDING, SUBCELLULAR LOCATION.
[12]"A role for NBR1 in autophagosomal degradation of ubiquitinated substrates."
Kirkin V., Lamark T., Sou Y.S., Bjorkoy G., Nunn J.L., Bruun J.A., Shvets E., McEwan D.G., Clausen T.H., Wild P., Bilusic I., Theurillat J.P., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T.
Mol. Cell 33:505-516(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH SQSTM1; MAP1LC3A; MAP1LC3B; MAP1LC3C; GABARAP; GABARAPL1 AND GABARAPL2, MUTAGENESIS OF ASP-50 AND TYR-732.
[13]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Solution structure of RSGI RUH-024, a PB1 domain, and of RSGI RUH-046, a UBA domain from human next to BRCA1 gene 1 protein (KIAA0049 protein) R923H variant."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 1-85 AND 916-956.
[15]"Crystal structure of the PB1 domain of NBR1."
Mueller S., Kursula I., Zou P., Wilmanns M.
FEBS Lett. 580:341-344(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) OF 1-85.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76952 mRNA. Translation: CAA54274.1.
D30756 mRNA. Translation: BAA06417.2. Different initiation.
AY450308 mRNA. Translation: AAS15047.1.
AC060780 Genomic DNA. No translation available.
AC109326 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60946.1.
BC009808 mRNA. Translation: AAH09808.1.
AF227189 mRNA. Translation: AAF74119.1.
U25764 Genomic DNA. Translation: AAA93228.1.
CCDSCCDS45694.1. [Q14596-1]
RefSeqNP_001278500.1. NM_001291571.1.
NP_001278501.1. NM_001291572.1.
NP_005890.2. NM_005899.4. [Q14596-1]
NP_114068.1. NM_031862.3. [Q14596-1]
UniGeneHs.277721.
Hs.708158.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJ6NMR-A1-85[»]
2BKFX-ray1.56A1-85[»]
2CP8NMR-A916-956[»]
2G4SX-ray2.15A1-85[»]
2L8JNMR-B726-738[»]
2MGWNMR-A913-959[»]
2MJ5NMR-B913-959[»]
4OLEX-ray2.52A/B/C/D365-485[»]
ProteinModelPortalQ14596.
SMRQ14596. Positions 1-85, 365-485, 913-957.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110253. 176 interactions.
IntActQ14596. 21 interactions.
MINTMINT-2806845.
STRING9606.ENSP00000343479.

PTM databases

PhosphoSiteQ14596.

Polymorphism databases

DMDM296439290.

Proteomic databases

MaxQBQ14596.
PaxDbQ14596.
PRIDEQ14596.

Protocols and materials databases

DNASU4077.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000341165; ENSP00000343479; ENSG00000188554. [Q14596-1]
ENST00000422280; ENSP00000411250; ENSG00000188554. [Q14596-1]
ENST00000586650; ENSP00000466071; ENSG00000188554.
ENST00000589872; ENSP00000467816; ENSG00000188554. [Q14596-2]
ENST00000590996; ENSP00000466667; ENSG00000188554. [Q14596-1]
GeneID4077.
KEGGhsa:4077.
UCSCuc010czd.3. human. [Q14596-1]
uc010whu.2. human. [Q14596-2]

Organism-specific databases

CTD4077.
GeneCardsGC17P041322.
HGNCHGNC:6746. NBR1.
HPAHPA022999.
HPA023999.
MIM166945. gene.
neXtProtNX_Q14596.
PharmGKBPA30510.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG239857.
HOGENOMHOG000220861.
HOVERGENHBG052578.
InParanoidQ14596.
KOK17987.
OMAAYKALFA.
OrthoDBEOG7FNC6V.
PhylomeDBQ14596.
TreeFamTF328428.

Gene expression databases

ArrayExpressQ14596.
BgeeQ14596.
CleanExHS_NBR1.
GenevestigatorQ14596.

Family and domain databases

InterProIPR000270. OPR_PB1.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000433. Znf_ZZ.
[Graphical view]
PfamPF00564. PB1. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTSM00666. PB1. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS50030. UBA. 1 hit.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNBR1. human.
EvolutionaryTraceQ14596.
GeneWikiNBR1.
GenomeRNAi4077.
NextBio15978.
PROQ14596.
SOURCESearch...

Entry information

Entry nameNBR1_HUMAN
AccessionPrimary (citable) accession number: Q14596
Secondary accession number(s): Q13173 expand/collapse secondary AC list , Q15026, Q5J7Q8, Q96GB6, Q9NRF7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM