Reviewed, UniProtKB/Swiss-Prot Q14582 (MAD4_HUMAN)

Names and origin

Protein names

Recommended name:
    Max-interacting transcriptional repressor MAD4
Alternative name(s):
    Max-associated protein 4
    MAX dimerization protein 4

Gene names

Name:	MXD4
Synonyms:	MAD4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation By similarity.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Traceable author statement. Source: ProtInc negative regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription corepressor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Max-interacting transcriptional repressor MAD4		PRO_0000127266
Regions
	Domain	67 – 106	40	Helix-loop-helix motif
	DNA binding	55 – 66	12	Basic motif
	Region	6 – 23	18	Interaction with SIN3A and SIN3B By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q14582-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: ACFAFB44EB0FE7A2	FASTA	209	23,528
	10 20 30 40 50 60 MELNSLLILL EAAEYLERRD REAEHGYASV LPFDGDFARE KTKAAGLVRK APNNRSSHNE 70 80 90 100 110 120 LEKHRRAKLR LYLEQLKQLV PLGPDSTRHT TLSLLKRAKV HIKKLEEQDR RALSIKEQLQ 130 140 150 160 170 180 QEHRFLKRRL EQLSVQSVER VRTDSTGSAV STDDSEQEVD IEGMEFGPGE LDSVGSSSDA 190 200 DDHYSLQSGT GGDSGFGPHC RRLGRPALS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pribill I., Barnes G.T., Chen J., Church D., Buckler A., Baxendale S., Bates G.P., Lehrach H., Gusella M.J., Duyao M.P., Ambrose C.M., Macdonald M.E., Gusella J.F. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and sequencing a novel human Max-associated protein, hMad4 and characterizing its transcriptional up-regulation in quiescent and senescent human fibroblasts." Qian J.-F., Wang X.-L., Wang E. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF040963 mRNA. Translation: AAB97009.1. AF082740 mRNA. Translation: AAF98553.1. BT019969 mRNA. Translation: AAV38772.1. BT019970 mRNA. Translation: AAV38773.1. Z49250 Genomic DNA. Translation: CAA89217.1. AL158068 Genomic DNA. Translation: CAM20460.1. BC068060 mRNA. Translation: AAH68060.1.
IPI	IPI00031584.
RefSeq	NP_006445.1.
UniGene	Hs.655020
3D structure databases
HSSP	HSSP built from PDB template 1NLW based on UniProtKB Q05195.
ModBase	Search...
Proteomic databases
PRIDE	Q14582.
Genome annotation databases
Ensembl	ENSG00000123933. Homo sapiens. [Contig view]
GeneID	10608.
KEGG	hsa:10608.
Organism-specific databases
GeneCards	GC04M002216.
HGNC	HGNC:13906. MXD4.
PharmGKB	PA134914966.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q14582.
HOVERGEN	Q14582.
OMA	Q14582. KAPNNRS.
Gene expression databases
ArrayExpress	Q14582.
Bgee	Q14582.
CleanEx	HS_MXD4.
GermOnline	ENSG00000123933. Homo sapiens.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR011598. HLH_DNA_bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40292.

Entry information

Entry name

MAD4_HUMAN

Accession

Primary (citable) accession number: Q14582
Secondary accession number(s): A2A335, Q5TZX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 15, 1999
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)