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Q14582 (MAD4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Max dimerization protein 4

Short name=Max dimerizer 4
Alternative name(s):
Class C basic helix-loop-helix protein 12
Short name=bHLHc12
Max-associated protein 4
Max-interacting transcriptional repressor MAD4
Gene names
Name:MXD4
Synonyms:BHLHC12, MAD4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation By similarity.

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Max dimerization protein 4
PRO_0000127266

Regions

Domain53 – 10553bHLH
Region6 – 2318Interaction with SIN3A and SIN3B By similarity

Sequences

Sequence LengthMass (Da)Tools
Q14582 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: ACFAFB44EB0FE7A2

FASTA20923,528
        10         20         30         40         50         60 
MELNSLLILL EAAEYLERRD REAEHGYASV LPFDGDFARE KTKAAGLVRK APNNRSSHNE 

        70         80         90        100        110        120 
LEKHRRAKLR LYLEQLKQLV PLGPDSTRHT TLSLLKRAKV HIKKLEEQDR RALSIKEQLQ 

       130        140        150        160        170        180 
QEHRFLKRRL EQLSVQSVER VRTDSTGSAV STDDSEQEVD IEGMEFGPGE LDSVGSSSDA 

       190        200 
DDHYSLQSGT GGDSGFGPHC RRLGRPALS 

« Hide

References

« Hide 'large scale' references
[1]Pribill I., Barnes G.T., Chen J., Church D., Buckler A., Baxendale S., Bates G.P., Lehrach H., Gusella M.J., Duyao M.P., Ambrose C.M., Macdonald M.E., Gusella J.F.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and sequencing a novel human Max-associated protein, hMad4 and characterizing its transcriptional up-regulation in quiescent and senescent human fibroblasts."
Qian J.-F., Wang X.-L., Wang E.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF040963 mRNA. Translation: AAB97009.1.
AF082740 mRNA. Translation: AAF98553.1.
BT019969 mRNA. Translation: AAV38772.1.
BT019970 mRNA. Translation: AAV38773.1.
Z49250 Genomic DNA. Translation: CAA89217.1.
AL158068 Genomic DNA. Translation: CAM20460.1.
BC068060 mRNA. Translation: AAH68060.1.
CCDSCCDS3361.1.
RefSeqNP_006445.1. NM_006454.2.
UniGeneHs.655020.

3D structure databases

ProteinModelPortalQ14582.
SMRQ14582. Positions 54-132.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115854. 6 interactions.
IntActQ14582. 1 interaction.
STRING9606.ENSP00000337889.

PTM databases

PhosphoSiteQ14582.

Polymorphism databases

DMDM6016524.

Proteomic databases

PaxDbQ14582.
PRIDEQ14582.

Protocols and materials databases

DNASU10608.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000337190; ENSP00000337889; ENSG00000123933.
GeneID10608.
KEGGhsa:10608.
UCSCuc003geu.1. human.

Organism-specific databases

CTD10608.
GeneCardsGC04M002249.
HGNCHGNC:13906. MXD4.
HPAHPA047538.
neXtProtNX_Q14582.
PharmGKBPA134914966.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG244990.
HOVERGENHBG006314.
InParanoidQ14582.
KOK09114.
OMAXSSHNEL.
OrthoDBEOG76T9V0.
PhylomeDBQ14582.
TreeFamTF315654.

Gene expression databases

ArrayExpressQ14582.
BgeeQ14582.
CleanExHS_MXD4.
GenevestigatorQ14582.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMXD4. human.
GeneWikiMXD4.
GenomeRNAi10608.
NextBio40292.
PROQ14582.

Entry information

Entry nameMAD4_HUMAN
AccessionPrimary (citable) accession number: Q14582
Secondary accession number(s): A2A335, Q5TZX4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 15, 1999
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM