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Q14582

- MAD4_HUMAN

UniProt

Q14582 - MAD4_HUMAN

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Protein

Max dimerization protein 4

Gene

MXD4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity).By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. transcription corepressor activity Source: ProtInc

GO - Biological processi

  1. negative regulation of cell proliferation Source: ProtInc
  2. negative regulation of transcription from RNA polymerase II promoter Source: ProtInc
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Max dimerization protein 4
Short name:
Max dimerizer 4
Alternative name(s):
Class C basic helix-loop-helix protein 12
Short name:
bHLHc12
Max-associated protein 4
Max-interacting transcriptional repressor MAD4
Gene namesi
Name:MXD4
Synonyms:BHLHC12, MAD4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:13906. MXD4.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134914966.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Max dimerization protein 4PRO_0000127266Add
BLAST

Proteomic databases

PaxDbiQ14582.
PRIDEiQ14582.

PTM databases

PhosphoSiteiQ14582.

Expressioni

Gene expression databases

BgeeiQ14582.
CleanExiHS_MXD4.
ExpressionAtlasiQ14582. baseline and differential.
GenevestigatoriQ14582.

Organism-specific databases

HPAiHPA047538.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with SIN3A AND SIN3B. Interacts with RNF17 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TCP10LQ8TDR46EBI-3943670,EBI-3923210

Protein-protein interaction databases

BioGridi115854. 6 interactions.
IntActiQ14582. 1 interaction.
STRINGi9606.ENSP00000337889.

Structurei

3D structure databases

ProteinModelPortaliQ14582.
SMRiQ14582. Positions 54-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 10553bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 2318Interaction with SIN3A and SIN3BBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG244990.
GeneTreeiENSGT00510000046360.
HOVERGENiHBG006314.
InParanoidiQ14582.
KOiK09114.
OMAiXSSHNEL.
OrthoDBiEOG76T9V0.
PhylomeDBiQ14582.
TreeFamiTF315654.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q14582-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELNSLLILL EAAEYLERRD REAEHGYASV LPFDGDFARE KTKAAGLVRK
60 70 80 90 100
APNNRSSHNE LEKHRRAKLR LYLEQLKQLV PLGPDSTRHT TLSLLKRAKV
110 120 130 140 150
HIKKLEEQDR RALSIKEQLQ QEHRFLKRRL EQLSVQSVER VRTDSTGSAV
160 170 180 190 200
STDDSEQEVD IEGMEFGPGE LDSVGSSSDA DDHYSLQSGT GGDSGFGPHC

RRLGRPALS
Length:209
Mass (Da):23,528
Last modified:July 15, 1999 - v2
Checksum:iACFAFB44EB0FE7A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF040963 mRNA. Translation: AAB97009.1.
AF082740 mRNA. Translation: AAF98553.1.
BT019969 mRNA. Translation: AAV38772.1.
BT019970 mRNA. Translation: AAV38773.1.
Z49250 Genomic DNA. Translation: CAA89217.1.
AL158068 Genomic DNA. Translation: CAM20460.1.
BC068060 mRNA. Translation: AAH68060.1.
CCDSiCCDS3361.1.
RefSeqiNP_006445.1. NM_006454.2.
UniGeneiHs.655020.

Genome annotation databases

EnsembliENST00000337190; ENSP00000337889; ENSG00000123933.
GeneIDi10608.
KEGGihsa:10608.
UCSCiuc003geu.1. human.

Polymorphism databases

DMDMi6016524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF040963 mRNA. Translation: AAB97009.1 .
AF082740 mRNA. Translation: AAF98553.1 .
BT019969 mRNA. Translation: AAV38772.1 .
BT019970 mRNA. Translation: AAV38773.1 .
Z49250 Genomic DNA. Translation: CAA89217.1 .
AL158068 Genomic DNA. Translation: CAM20460.1 .
BC068060 mRNA. Translation: AAH68060.1 .
CCDSi CCDS3361.1.
RefSeqi NP_006445.1. NM_006454.2.
UniGenei Hs.655020.

3D structure databases

ProteinModelPortali Q14582.
SMRi Q14582. Positions 54-132.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115854. 6 interactions.
IntActi Q14582. 1 interaction.
STRINGi 9606.ENSP00000337889.

PTM databases

PhosphoSitei Q14582.

Polymorphism databases

DMDMi 6016524.

Proteomic databases

PaxDbi Q14582.
PRIDEi Q14582.

Protocols and materials databases

DNASUi 10608.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000337190 ; ENSP00000337889 ; ENSG00000123933 .
GeneIDi 10608.
KEGGi hsa:10608.
UCSCi uc003geu.1. human.

Organism-specific databases

CTDi 10608.
GeneCardsi GC04M002249.
HGNCi HGNC:13906. MXD4.
HPAi HPA047538.
neXtProti NX_Q14582.
PharmGKBi PA134914966.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG244990.
GeneTreei ENSGT00510000046360.
HOVERGENi HBG006314.
InParanoidi Q14582.
KOi K09114.
OMAi XSSHNEL.
OrthoDBi EOG76T9V0.
PhylomeDBi Q14582.
TreeFami TF315654.

Miscellaneous databases

ChiTaRSi MXD4. human.
GeneWikii MXD4.
GenomeRNAii 10608.
NextBioi 40292.
PROi Q14582.

Gene expression databases

Bgeei Q14582.
CleanExi HS_MXD4.
ExpressionAtlasi Q14582. baseline and differential.
Genevestigatori Q14582.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and sequencing a novel human Max-associated protein, hMad4 and characterizing its transcriptional up-regulation in quiescent and senescent human fibroblasts."
    Qian J.-F., Wang X.-L., Wang E.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiMAD4_HUMAN
AccessioniPrimary (citable) accession number: Q14582
Secondary accession number(s): A2A335, Q5TZX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3