ID   ELAV3_HUMAN             Reviewed;         367 AA.
AC   Q14576; Q16135; Q96CL8; Q96QS9;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2002, sequence version 3.
DT   03-NOV-2009, entry version 89.
DE   RecName: Full=ELAV-like protein 3;
DE   AltName: Full=Hu-antigen C;
DE            Short=HuC;
DE   AltName: Full=Paraneoplastic cerebellar degeneration-associated antigen;
DE   AltName: Full=Paraneoplastic limbic encephalitis antigen 21;
GN   Name=ELAVL3; Synonyms=HUC, PLE21;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC   TISSUE=Brain;
RA   Manley T., Furneaux H.M.;
RL   Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Zhang B., Yuan J.G., Qiang B.Q.;
RT   "Isolation of HuC cDNA.";
RL   Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Brain;
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J.,
RA   Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M.,
RA   Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E.,
RA   Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M.,
RA   Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C.,
RA   Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M.,
RA   Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T.,
RA   Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H.,
RA   Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S.,
RA   Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J.,
RA   Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M.,
RA   Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J.,
RA   Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D.,
RA   Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A.,
RA   Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I.,
RA   Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 9-367 (ISOFORM 2).
RC   TISSUE=Hippocampus;
RX   MEDLINE=94197705; PubMed=7511893; DOI=10.1006/bbrc.1994.1358;
RA   Sakai K., Gofuku M., Kitagawa Y., Ogasawara T., Hirose G.,
RA   Yamazaki M., Koh C.S., Yanagisawa N., Steinman L.;
RT   "A hippocampal protein associated with paraneoplastic neurologic
RT   syndrome and small cell lung carcinoma.";
RL   Biochem. Biophys. Res. Commun. 199:1200-1208(1994).
RN   [6]
RP   FUNCTION.
RX   PubMed=10710437; DOI=10.1093/nar/28.7.e20;
RA   King P.H.;
RT   "RNA-binding analyses of HuC and HuD with the VEGF and c-myc 3'-
RT   untranslated regions using a novel ELISA-based assay.";
RL   Nucleic Acids Res. 28:E20-E20(2000).
CC   -!- FUNCTION: Binds to AU-rich sequences (AREs) of target mRNAs,
CC       including VEGF mRNA. May also bind poly-A tracts via RRM 3 (By
CC       similarity). May be involved in neuronal differentiation and
CC       maintenance.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q14576-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q14576-2; Sequence=VSP_005789;
CC   -!- TISSUE SPECIFICITY: Brain specific.
CC   -!- DOMAIN: RRM 1 and RRM 2 bind cooperatively to AU-rich sequences in
CC       target mRNAs. RRM 3 binds to poly-A mRNA sequences (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the RRM elav family.
CC   -!- SIMILARITY: Contains 3 RRM (RNA recognition motif) domains.
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DR   EMBL; L26405; AAA58677.1; -; mRNA.
DR   EMBL; AY036909; AAK67714.1; -; mRNA.
DR   EMBL; AC008481; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC014144; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; D26158; BAA21838.1; -; mRNA.
DR   IPI; IPI00031552; -.
DR   IPI; IPI00140449; -.
DR   RefSeq; NP_001411.2; -.
DR   RefSeq; NP_115657.2; -.
DR   UniGene; Hs.1701; -.
DR   HSSP; Q60900; 1D8Z.
DR   SMR; Q14576; 36-208.
DR   IntAct; Q14576; 1.
DR   STRING; Q14576; -.
DR   PhosphoSite; Q14576; -.
DR   PRIDE; Q14576; -.
DR   Ensembl; ENST00000359227; ENSP00000352162; ENSG00000196361; Homo sapiens.
DR   Ensembl; ENST00000438662; ENSP00000390878; ENSG00000196361; Homo sapiens.
DR   GeneID; 1995; -.
DR   KEGG; hsa:1995; -.
DR   UCSC; uc002mrx.1; human.
DR   UCSC; uc002mry.1; human.
DR   CTD; 1995; -.
DR   GeneCards; GC19M011426; -.
DR   H-InvDB; HIX0014771; -.
DR   HGNC; HGNC:3314; ELAVL3.
DR   HPA; CAB013066; -.
DR   MIM; 603458; gene.
DR   PharmGKB; PA27742; -.
DR   HOGENOM; Q14576; -.
DR   HOVERGEN; Q14576; -.
DR   OMA; ISTMETQ; -.
DR   NextBio; 8067; -.
DR   ArrayExpress; Q14576; -.
DR   Bgee; Q14576; -.
DR   CleanEx; HS_ELAVL3; -.
DR   Genevestigator; Q14576; -.
DR   GermOnline; ENSG00000196361; Homo sapiens.
DR   GO; GO:0017091; F:AU-rich element binding; IDA:UniProtKB.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   InterPro; IPR012677; a_b_plait_nuc_bd.
DR   InterPro; IPR006548; ELAD_HUD_SF.
DR   InterPro; IPR002343; Hud_Sxl_RNA.
DR   InterPro; IPR000504; RRM_RNP1.
DR   Gene3D; G3DSA:3.30.70.330; a_b_plait_nuc_bd; 3.
DR   Pfam; PF00076; RRM_1; 3.
DR   PRINTS; PR00961; HUDSXLRNA.
DR   SMART; SM00360; RRM; 3.
DR   TIGRFAMs; TIGR01661; ELAV_HUD_SF; 1.
DR   PROSITE; PS50102; RRM; 3.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Developmental protein;
KW   Differentiation; Neurogenesis; Repeat; RNA-binding.
FT   CHAIN         1    367       ELAV-like protein 3.
FT                                /FTId=PRO_0000081581.
FT   DOMAIN       39    117       RRM 1.
FT   DOMAIN      125    205       RRM 2.
FT   DOMAIN      284    362       RRM 3.
FT   VAR_SEQ     251    257       Missing (in isoform 2).
FT                                /FTId=VSP_005789.
FT   CONFLICT     20     25       PALPNG -> RPAQR (in Ref. 4; BAA21838).
FT   CONFLICT     78     79       QS -> RD (in Ref. 1; AAA58677 and 2;
FT                                AAK67714).
FT   CONFLICT     88     88       S -> P (in Ref. 1; AAA58677 and 2;
FT                                AAK67714).
FT   CONFLICT    195    195       A -> R (in Ref. 4; BAA21838).
FT   CONFLICT    248    248       G -> A (in Ref. 4; BAA21838).
FT   CONFLICT    282    282       Missing (in Ref. 1; AAA58677 and 4;
FT                                BAA21838).
FT   CONFLICT    295    297       ADE -> PDQ (in Ref. 4; BAA21838).
FT   CONFLICT    330    330       V -> M (in Ref. 4; BAA21838).
FT   CONFLICT    351    351       G -> A (in Ref. 1; AAA58677).
FT   CONFLICT    352    352       E -> Q (in Ref. 4; BAA21838).
SQ   SEQUENCE   367 AA;  39547 MW;  03FB3BD4D112B326 CRC64;
     MVTQILGAME SQVGGGPAGP ALPNGPLLGT NGATDDSKTN LIVNYLPQNM TQDEFKSLFG
     SIGDIESCKL VRDKITGQSL GYGFVNYSDP NDADKAINTL NGLKLQTKTI KVSYARPSSA
     SIRDANLYVS GLPKTMSQKE MEQLFSQYGR IITSRILVDQ VTGVSRGVGF IRFDKRIEAE
     EAIKGLNGQK PLGAAEPITV KFANNPSQKT GQALLTHLYQ SSARRYAGPL HHQTQRFRLD
     NLLNMAYGVK SPLSLIARFS PIAIDGMSGL AGVGLSGGAA GAGWCIFVYN LSPEADESVL
     WQLFGPFGAV TNVKVIRDFT TNKCKGFGFV TMTNYDEAAM AIASLNGYRL GERVLQVSFK
     TSKQHKA
//