Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q14562

- DHX8_HUMAN

UniProt

Q14562 - DHX8_HUMAN

Protein

ATP-dependent RNA helicase DHX8

Gene

DHX8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi588 – 5958ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: ProtInc
    3. identical protein binding Source: IntAct
    4. poly(A) RNA binding Source: UniProtKB
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. mRNA splicing, via spliceosome Source: UniProtKB
    3. RNA processing Source: ProtInc
    4. RNA splicing Source: ProtInc

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent RNA helicase DHX8 (EC:3.6.4.13)
    Alternative name(s):
    DEAH box protein 8
    RNA helicase HRH1
    Gene namesi
    Name:DHX8
    Synonyms:DDX8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:2749. DHX8.

    Subcellular locationi

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nucleus Source: HPA
    3. spliceosomal complex Source: ProtInc

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi594 – 5941K → E in GET; inhibition of pre-mRNA splicing and nuclear export of unspliced RNA.
    Mutagenesisi717 – 7171S → L in LAT; inhibition of pre-mRNA splicing and nuclear export of unspliced RNA.

    Organism-specific databases

    PharmGKBiPA27231.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12201220ATP-dependent RNA helicase DHX8PRO_0000055131Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei456 – 4561Phosphoserine1 Publication
    Modified residuei460 – 4601Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ14562.
    PaxDbiQ14562.
    PeptideAtlasiQ14562.
    PRIDEiQ14562.

    PTM databases

    PhosphoSiteiQ14562.

    Expressioni

    Gene expression databases

    ArrayExpressiQ14562.
    BgeeiQ14562.
    CleanExiHS_DHX8.
    GenevestigatoriQ14562.

    Organism-specific databases

    HPAiHPA049285.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-2511477,EBI-2511477
    CHERPQ8IWX82EBI-2511477,EBI-2555370
    ISY1Q9ULR02EBI-2511477,EBI-2557660
    NKAPQ8N5F72EBI-2511477,EBI-721539

    Protein-protein interaction databases

    BioGridi108024. 31 interactions.
    IntActiQ14562. 20 interactions.
    MINTiMINT-3029791.
    STRINGi9606.ENSP00000262415.

    Structurei

    Secondary structure

    1
    1220
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi267 – 2759
    Beta strandi280 – 2845
    Beta strandi286 – 2894
    Beta strandi292 – 2954
    Helixi297 – 2993
    Beta strandi301 – 3044
    Helixi309 – 3124
    Beta strandi318 – 32710
    Beta strandi330 – 3356
    Turni340 – 3423
    Helixi950 – 96314
    Helixi975 – 9806
    Helixi987 – 99812
    Helixi1002 – 101211
    Helixi1022 – 10243
    Helixi1025 – 10339
    Helixi1041 – 105414
    Turni1055 – 10573
    Helixi1059 – 10646
    Helixi1069 – 108820
    Helixi1101 – 111111
    Helixi1112 – 11143
    Beta strandi1115 – 11184
    Beta strandi1120 – 11267
    Turni1127 – 11293
    Beta strandi1132 – 11354
    Turni1140 – 11434
    Beta strandi1147 – 116721
    Helixi1170 – 11767
    Turni1178 – 11803

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EQSNMR-A260-355[»]
    3I4UX-ray2.10A950-1183[»]
    ProteinModelPortaliQ14562.
    SMRiQ14562. Positions 260-355, 556-1198.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ14562.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini265 – 33672S1 motifPROSITE-ProRule annotationAdd
    BLAST
    Domaini575 – 738164Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini756 – 936181Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi685 – 6884DEAH box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi172 – 1754Poly-Lys
    Compositional biasi176 – 22853Arg/Ser-rich (RS domain)Add
    BLAST

    Domaini

    The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 S1 motif domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    HOGENOMiHOG000175261.
    HOVERGENiHBG039428.
    InParanoidiQ14562.
    KOiK12818.
    OMAiFVQIRTL.
    PhylomeDBiQ14562.
    TreeFamiTF300509.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q14562-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA     50
    EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST 100
    SKDPVVKPKT EKEKLKELFP VLCQPDNPSV RTMLDEDDVK VAVDVLKELE 150
    ALMPSAAGQE KQRDAEHRDR TKKKKRSRSR DRNRDRDRDR ERNRDRDHKR 200
    RHRSRSRSRS RTRERNKVKS RYRSRSRSQS PPKDRKDRDK YGERNLDRWR 250
    DKHVDRPPPE EPTIGDIYNG KVTSIMQFGC FVQLEGLRKR WEGLVHISEL 300
    RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR 350
    RRNLVGETNE ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW 400
    EIKQMIAANV LSKEEFPDFD EETGILPKVD DEEDEDLEIE LVEEEPPFLR 450
    GHTKQSMDMS PIKIVKNPDG SLSQAAMMQS ALAKERRELK QAQREAEMDS 500
    IPMGLNKHWV DPLPDAEGRQ IAANMRGIGM MPNDIPEWKK HAFGGNKASY 550
    GKKTQMSILE QRESLPIYKL KEQLVQAVHD NQILIVIGET GSGKTTQITQ 600
    YLAEAGYTSR GKIGCTQPRR VAAMSVAKRV SEEFGCCLGQ EVGYTIRFED 650
    CTSPETVIKY MTDGMLLREC LIDPDLTQYA IIMLDEAHER TIHTDVLFGL 700
    LKKTVQKRQD MKLIVTSATL DAVKFSQYFY EAPIFTIPGR TYPVEILYTK 750
    EPETDYLDAS LITVMQIHLT EPPGDILVFL TGQEEIDTAC EILYERMKSL 800
    GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG 850
    IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAGR AGRTGPGKCY 900
    RLYTERAYRD EMLTTNVPEI QRTNLASTVL SLKAMGINDL LSFDFMDAPP 950
    METLITAMEQ LYTLGALDDE GLLTRLGRRM AEFPLEPMLC KMLIMSVHLG 1000
    CSEEMLTIVS MLSVQNVFYR PKDKQALADQ KKAKFHQTEG DHLTLLAVYN 1050
    SWKNNKFSNP WCYENFIQAR SLRRAQDIRK QMLGIMDRHK LDVVSCGKST 1100
    VRVQKAICSG FFRNAAKKDP QEGYRTLIDQ QVVYIHPSSA LFNRQPEWVV 1150
    YHELVLTTKE YMREVTTIDP RWLVEFAPAF FKVSDPTKLS KQKKQQRLEP 1200
    LYNRYEEPNA WRISRAFRRR 1220
    Length:1,220
    Mass (Da):139,315
    Last modified:November 1, 1996 - v1
    Checksum:i17C1602A73A0EF24
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1069 – 10691A → G.
    Corresponds to variant rs34285079 [ dbSNP | Ensembl ].
    VAR_052174

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50487 mRNA. Translation: BAA09078.1.
    CCDSiCCDS11464.1.
    PIRiA56236.
    RefSeqiNP_004932.1. NM_004941.1.
    UniGeneiHs.463105.

    Genome annotation databases

    EnsembliENST00000262415; ENSP00000262415; ENSG00000067596.
    GeneIDi1659.
    KEGGihsa:1659.
    UCSCiuc002idu.1. human.

    Polymorphism databases

    DMDMi3023637.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50487 mRNA. Translation: BAA09078.1 .
    CCDSi CCDS11464.1.
    PIRi A56236.
    RefSeqi NP_004932.1. NM_004941.1.
    UniGenei Hs.463105.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EQS NMR - A 260-355 [» ]
    3I4U X-ray 2.10 A 950-1183 [» ]
    ProteinModelPortali Q14562.
    SMRi Q14562. Positions 260-355, 556-1198.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108024. 31 interactions.
    IntActi Q14562. 20 interactions.
    MINTi MINT-3029791.
    STRINGi 9606.ENSP00000262415.

    PTM databases

    PhosphoSitei Q14562.

    Polymorphism databases

    DMDMi 3023637.

    Proteomic databases

    MaxQBi Q14562.
    PaxDbi Q14562.
    PeptideAtlasi Q14562.
    PRIDEi Q14562.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262415 ; ENSP00000262415 ; ENSG00000067596 .
    GeneIDi 1659.
    KEGGi hsa:1659.
    UCSCi uc002idu.1. human.

    Organism-specific databases

    CTDi 1659.
    GeneCardsi GC17P041572.
    HGNCi HGNC:2749. DHX8.
    HPAi HPA049285.
    MIMi 600396. gene.
    neXtProti NX_Q14562.
    PharmGKBi PA27231.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1643.
    HOGENOMi HOG000175261.
    HOVERGENi HBG039428.
    InParanoidi Q14562.
    KOi K12818.
    OMAi FVQIRTL.
    PhylomeDBi Q14562.
    TreeFami TF300509.

    Miscellaneous databases

    EvolutionaryTracei Q14562.
    GenomeRNAii 1659.
    NextBioi 6828.
    PROi Q14562.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14562.
    Bgeei Q14562.
    CleanExi HS_DHX8.
    Genevestigatori Q14562.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22."
      Ono Y., Ohno M., Shimura Y.
      Mol. Cell. Biol. 14:7611-7620(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome."
      Ohno M., Shimura Y.
      Genes Dev. 10:997-1007(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    4. "Novel function of beta-arrestin2 in the nucleus of mature spermatozoa."
      Neuhaus E.M., Mashukova A., Barbour J., Wolters D., Hatt H.
      J. Cell Sci. 119:3047-3056(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARRB2.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456 AND SER-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Solution structure of the S1 RNA binding domain of human ATP-dependent RNA helicase DHX8."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2007) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 260-355.

    Entry informationi

    Entry nameiDHX8_HUMAN
    AccessioniPrimary (citable) accession number: Q14562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 143 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3