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Protein

ATP-dependent RNA helicase DHX8

Gene

DHX8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi588 – 595ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: ProtInc
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA processing Source: ProtInc
  • RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00911-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX8 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 8
RNA helicase HRH1
Gene namesi
Name:DHX8
Synonyms:DDX8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:2749. DHX8.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cytoplasm Source: GO_Central
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
  • spliceosomal complex Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi594K → E in GET; inhibition of pre-mRNA splicing and nuclear export of unspliced RNA. 1
Mutagenesisi717S → L in LAT; inhibition of pre-mRNA splicing and nuclear export of unspliced RNA. 1

Organism-specific databases

DisGeNETi1659.
OpenTargetsiENSG00000067596.
PharmGKBiPA27231.

Polymorphism and mutation databases

BioMutaiDHX8.
DMDMi3023637.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551311 – 1220ATP-dependent RNA helicase DHX8Add BLAST1220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphoserineCombined sources1
Modified residuei460PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ14562.
MaxQBiQ14562.
PaxDbiQ14562.
PeptideAtlasiQ14562.
PRIDEiQ14562.

PTM databases

iPTMnetiQ14562.
PhosphoSitePlusiQ14562.

Expressioni

Gene expression databases

BgeeiENSG00000067596.
CleanExiHS_DHX8.
ExpressionAtlasiQ14562. baseline and differential.
GenevisibleiQ14562. HS.

Organism-specific databases

HPAiHPA049285.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2511477,EBI-2511477
CHERPQ8IWX82EBI-2511477,EBI-2555370
ISY1Q9ULR02EBI-2511477,EBI-2557660
NKAPQ8N5F72EBI-2511477,EBI-721539

Protein-protein interaction databases

BioGridi108024. 75 interactors.
IntActiQ14562. 27 interactors.
MINTiMINT-3029791.
STRINGi9606.ENSP00000262415.

Structurei

Secondary structure

11220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi267 – 275Combined sources9
Beta strandi280 – 284Combined sources5
Beta strandi286 – 289Combined sources4
Beta strandi292 – 295Combined sources4
Helixi297 – 299Combined sources3
Beta strandi301 – 304Combined sources4
Helixi309 – 312Combined sources4
Beta strandi318 – 327Combined sources10
Beta strandi330 – 335Combined sources6
Turni340 – 342Combined sources3
Helixi950 – 963Combined sources14
Helixi975 – 980Combined sources6
Helixi987 – 998Combined sources12
Helixi1002 – 1012Combined sources11
Helixi1022 – 1024Combined sources3
Helixi1025 – 1033Combined sources9
Helixi1041 – 1054Combined sources14
Turni1055 – 1057Combined sources3
Helixi1059 – 1064Combined sources6
Helixi1069 – 1088Combined sources20
Helixi1101 – 1111Combined sources11
Helixi1112 – 1114Combined sources3
Beta strandi1115 – 1118Combined sources4
Beta strandi1120 – 1126Combined sources7
Turni1127 – 1129Combined sources3
Beta strandi1132 – 1135Combined sources4
Turni1140 – 1143Combined sources4
Beta strandi1147 – 1167Combined sources21
Helixi1170 – 1176Combined sources7
Turni1178 – 1180Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EQSNMR-A260-355[»]
3I4UX-ray2.10A950-1183[»]
ProteinModelPortaliQ14562.
SMRiQ14562.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ14562.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini265 – 336S1 motifPROSITE-ProRule annotationAdd BLAST72
Domaini575 – 738Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST164
Domaini756 – 936Helicase C-terminalPROSITE-ProRule annotationAdd BLAST181

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi685 – 688DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi172 – 175Poly-Lys4
Compositional biasi176 – 228Arg/Ser-rich (RS domain)Add BLAST53

Domaini

The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0922. Eukaryota.
COG1185. LUCA.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ14562.
KOiK12818.
OMAiPRPMANP.
OrthoDBiEOG091G030Q.
PhylomeDBiQ14562.
TreeFamiTF300509.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q14562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA
60 70 80 90 100
EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST
110 120 130 140 150
SKDPVVKPKT EKEKLKELFP VLCQPDNPSV RTMLDEDDVK VAVDVLKELE
160 170 180 190 200
ALMPSAAGQE KQRDAEHRDR TKKKKRSRSR DRNRDRDRDR ERNRDRDHKR
210 220 230 240 250
RHRSRSRSRS RTRERNKVKS RYRSRSRSQS PPKDRKDRDK YGERNLDRWR
260 270 280 290 300
DKHVDRPPPE EPTIGDIYNG KVTSIMQFGC FVQLEGLRKR WEGLVHISEL
310 320 330 340 350
RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR
360 370 380 390 400
RRNLVGETNE ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW
410 420 430 440 450
EIKQMIAANV LSKEEFPDFD EETGILPKVD DEEDEDLEIE LVEEEPPFLR
460 470 480 490 500
GHTKQSMDMS PIKIVKNPDG SLSQAAMMQS ALAKERRELK QAQREAEMDS
510 520 530 540 550
IPMGLNKHWV DPLPDAEGRQ IAANMRGIGM MPNDIPEWKK HAFGGNKASY
560 570 580 590 600
GKKTQMSILE QRESLPIYKL KEQLVQAVHD NQILIVIGET GSGKTTQITQ
610 620 630 640 650
YLAEAGYTSR GKIGCTQPRR VAAMSVAKRV SEEFGCCLGQ EVGYTIRFED
660 670 680 690 700
CTSPETVIKY MTDGMLLREC LIDPDLTQYA IIMLDEAHER TIHTDVLFGL
710 720 730 740 750
LKKTVQKRQD MKLIVTSATL DAVKFSQYFY EAPIFTIPGR TYPVEILYTK
760 770 780 790 800
EPETDYLDAS LITVMQIHLT EPPGDILVFL TGQEEIDTAC EILYERMKSL
810 820 830 840 850
GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG
860 870 880 890 900
IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAGR AGRTGPGKCY
910 920 930 940 950
RLYTERAYRD EMLTTNVPEI QRTNLASTVL SLKAMGINDL LSFDFMDAPP
960 970 980 990 1000
METLITAMEQ LYTLGALDDE GLLTRLGRRM AEFPLEPMLC KMLIMSVHLG
1010 1020 1030 1040 1050
CSEEMLTIVS MLSVQNVFYR PKDKQALADQ KKAKFHQTEG DHLTLLAVYN
1060 1070 1080 1090 1100
SWKNNKFSNP WCYENFIQAR SLRRAQDIRK QMLGIMDRHK LDVVSCGKST
1110 1120 1130 1140 1150
VRVQKAICSG FFRNAAKKDP QEGYRTLIDQ QVVYIHPSSA LFNRQPEWVV
1160 1170 1180 1190 1200
YHELVLTTKE YMREVTTIDP RWLVEFAPAF FKVSDPTKLS KQKKQQRLEP
1210 1220
LYNRYEEPNA WRISRAFRRR
Length:1,220
Mass (Da):139,315
Last modified:November 1, 1996 - v1
Checksum:i17C1602A73A0EF24
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0521741069A → G.Corresponds to variant rs34285079dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50487 mRNA. Translation: BAA09078.1.
CCDSiCCDS11464.1.
PIRiA56236.
RefSeqiNP_001289552.1. NM_001302623.1.
NP_004932.1. NM_004941.2.
UniGeneiHs.463105.

Genome annotation databases

EnsembliENST00000262415; ENSP00000262415; ENSG00000067596.
GeneIDi1659.
KEGGihsa:1659.
UCSCiuc002idu.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50487 mRNA. Translation: BAA09078.1.
CCDSiCCDS11464.1.
PIRiA56236.
RefSeqiNP_001289552.1. NM_001302623.1.
NP_004932.1. NM_004941.2.
UniGeneiHs.463105.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EQSNMR-A260-355[»]
3I4UX-ray2.10A950-1183[»]
ProteinModelPortaliQ14562.
SMRiQ14562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108024. 75 interactors.
IntActiQ14562. 27 interactors.
MINTiMINT-3029791.
STRINGi9606.ENSP00000262415.

PTM databases

iPTMnetiQ14562.
PhosphoSitePlusiQ14562.

Polymorphism and mutation databases

BioMutaiDHX8.
DMDMi3023637.

Proteomic databases

EPDiQ14562.
MaxQBiQ14562.
PaxDbiQ14562.
PeptideAtlasiQ14562.
PRIDEiQ14562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262415; ENSP00000262415; ENSG00000067596.
GeneIDi1659.
KEGGihsa:1659.
UCSCiuc002idu.2. human.

Organism-specific databases

CTDi1659.
DisGeNETi1659.
GeneCardsiDHX8.
HGNCiHGNC:2749. DHX8.
HPAiHPA049285.
MIMi600396. gene.
neXtProtiNX_Q14562.
OpenTargetsiENSG00000067596.
PharmGKBiPA27231.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0922. Eukaryota.
COG1185. LUCA.
COG1643. LUCA.
GeneTreeiENSGT00840000129715.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ14562.
KOiK12818.
OMAiPRPMANP.
OrthoDBiEOG091G030Q.
PhylomeDBiQ14562.
TreeFamiTF300509.

Enzyme and pathway databases

BioCyciZFISH:HS00911-MONOMER.

Miscellaneous databases

ChiTaRSiDHX8. human.
EvolutionaryTraceiQ14562.
GenomeRNAii1659.
PROiQ14562.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000067596.
CleanExiHS_DHX8.
ExpressionAtlasiQ14562. baseline and differential.
GenevisibleiQ14562. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX8_HUMAN
AccessioniPrimary (citable) accession number: Q14562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.