Q14562 (DHX8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DHX8
EC=3.6.4.13

Alternative name(s):
DEAH box protein 8
RNA helicase HRH1

Gene names

Name:	DHX8
Synonyms:	DDX8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Ref.2
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Identified in the spliceosome C complex. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Ref.3 Ref.4
Subcellular location	Nucleus.
Domain	The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred by curator Ref.3. Source: UniProtKB
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent RNA helicase activity Traceable author statement Ref.1. Source: ProtInc RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1220

1220

ATP-dependent RNA helicase DHX8

PRO_0000055131

Regions

Domain

265 – 336

72

S1 motif

Domain

575 – 738

164

Helicase ATP-binding

Domain

756 – 936

181

Helicase C-terminal

Nucleotide binding

588 – 595

8

ATP By similarity

Motif

685 – 688

4

DEAH box

Compositional bias

172 – 175

4

Poly-Lys

Compositional bias

176 – 228

53

Arg/Ser-rich (RS domain)

Amino acid modifications

Modified residue

456

1

Phosphoserine Ref.8

Modified residue

460

1

Phosphoserine Ref.6 Ref.8

Natural variations

Natural variant

1069

1

A → G.
Corresponds to variant rs34285079 [ dbSNP | Ensembl ].

VAR_052174

Experimental info

Mutagenesis

594

1

K → E in GET; inhibition of pre-mRNA splicing and nuclear export of unspliced RNA.

Mutagenesis

717

1

S → L in LAT; inhibition of pre-mRNA splicing and nuclear export of unspliced RNA.

Secondary structure

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1220

Helix Strand Turn

Details...

Beta strand

267 – 275

9

Beta strand

280 – 284

5

Beta strand

286 – 289

4

Beta strand

292 – 295

4

Helix

297 – 299

3

Beta strand

301 – 304

4

Helix

309 – 312

4

Beta strand

318 – 327

10

Beta strand

330 – 335

6

Turn

340 – 342

3

Helix

950 – 963

14

Helix

975 – 980

6

Helix

987 – 998

12

Helix

1002 – 1012

11

Helix

1022 – 1024

3

Helix

1025 – 1033

9

Helix

1041 – 1054

14

Turn

1055 – 1057

3

Helix

1059 – 1064

6

Helix

1069 – 1088

20

Helix

1101 – 1111

11

Helix

1112 – 1114

3

Beta strand

1115 – 1118

4

Beta strand

1120 – 1126

7

Turn

1127 – 1129

3

Beta strand

1132 – 1135

4

Turn

1140 – 1143

4

Beta strand

1147 – 1167

21

Helix

1170 – 1176

7

Turn

1178 – 1180

3

Sequences

	Sequence		Length	Mass (Da)
	Q14562 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 17C1602A73A0EF24	FASTA	1,220	139,315
	10 20 30 40 50 60 MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN 70 80 90 100 110 120 TTFDTFKASL VKNGAEFTDS LISNLLRLIQ TMRPPAKPST SKDPVVKPKT EKEKLKELFP 130 140 150 160 170 180 VLCQPDNPSV RTMLDEDDVK VAVDVLKELE ALMPSAAGQE KQRDAEHRDR TKKKKRSRSR 190 200 210 220 230 240 DRNRDRDRDR ERNRDRDHKR RHRSRSRSRS RTRERNKVKS RYRSRSRSQS PPKDRKDRDK 250 260 270 280 290 300 YGERNLDRWR DKHVDRPPPE EPTIGDIYNG KVTSIMQFGC FVQLEGLRKR WEGLVHISEL 310 320 330 340 350 360 RREGRVANVA DVVSKGQRVK VKVLSFTGTK TSLSMKDVDQ ETGEDLNPNR RRNLVGETNE 370 380 390 400 410 420 ETSMRNPDRP THLSLVSAPE VEDDSLERKR LTRISDPEKW EIKQMIAANV LSKEEFPDFD 430 440 450 460 470 480 EETGILPKVD DEEDEDLEIE LVEEEPPFLR GHTKQSMDMS PIKIVKNPDG SLSQAAMMQS 490 500 510 520 530 540 ALAKERRELK QAQREAEMDS IPMGLNKHWV DPLPDAEGRQ IAANMRGIGM MPNDIPEWKK 550 560 570 580 590 600 HAFGGNKASY GKKTQMSILE QRESLPIYKL KEQLVQAVHD NQILIVIGET GSGKTTQITQ 610 620 630 640 650 660 YLAEAGYTSR GKIGCTQPRR VAAMSVAKRV SEEFGCCLGQ EVGYTIRFED CTSPETVIKY 670 680 690 700 710 720 MTDGMLLREC LIDPDLTQYA IIMLDEAHER TIHTDVLFGL LKKTVQKRQD MKLIVTSATL 730 740 750 760 770 780 DAVKFSQYFY EAPIFTIPGR TYPVEILYTK EPETDYLDAS LITVMQIHLT EPPGDILVFL 790 800 810 820 830 840 TGQEEIDTAC EILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN 850 860 870 880 890 900 IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAGR AGRTGPGKCY 910 920 930 940 950 960 RLYTERAYRD EMLTTNVPEI QRTNLASTVL SLKAMGINDL LSFDFMDAPP METLITAMEQ 970 980 990 1000 1010 1020 LYTLGALDDE GLLTRLGRRM AEFPLEPMLC KMLIMSVHLG CSEEMLTIVS MLSVQNVFYR 1030 1040 1050 1060 1070 1080 PKDKQALADQ KKAKFHQTEG DHLTLLAVYN SWKNNKFSNP WCYENFIQAR SLRRAQDIRK 1090 1100 1110 1120 1130 1140 QMLGIMDRHK LDVVSCGKST VRVQKAICSG FFRNAAKKDP QEGYRTLIDQ QVVYIHPSSA 1150 1160 1170 1180 1190 1200 LFNRQPEWVV YHELVLTTKE YMREVTTIDP RWLVEFAPAF FKVSDPTKLS KQKKQQRLEP 1210 1220 LYNRYEEPNA WRISRAFRRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22." Ono Y., Ohno M., Shimura Y. Mol. Cell. Biol. 14:7611-7620(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome." Ohno M., Shimura Y. Genes Dev. 10:997-1007(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[4]	"Novel function of beta-arrestin2 in the nucleus of mature spermatozoa." Neuhaus E.M., Mashukova A., Barbour J., Wolters D., Hatt H. J. Cell Sci. 119:3047-3056(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARRB2.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-460, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456 AND SER-460, MASS SPECTROMETRY.
[9]	"Solution structure of the S1 RNA binding domain of human ATP-dependent RNA helicase DHX8." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 260-355.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50487 mRNA. Translation: BAA09078.1.

IPI

IPI00031508.

PIR

A56236.

RefSeq

NP_004932.1. NM_004941.1.

UniGene

Hs.463105.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EQS	NMR	-	A	260-355	[»]
3I4U	X-ray	2.10	A	950-1183	[»]

ProteinModelPortal

Q14562.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14562. 4 interactions.

STRING

9606.ENSP00000262415.

PTM databases

PhosphoSite

Q14562.

Polymorphism databases

DMDM

3023637.

Proteomic databases

PaxDb

Q14562.

PeptideAtlas

Q14562.

PRIDE

Q14562.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000262415; ENSP00000262415; ENSG00000067596.

GeneID

1659.

KEGG

hsa:1659.

UCSC

uc002idu.1. human.

Organism-specific databases

CTD

1659.

GeneCards

GC17P041572.

HGNC

HGNC:2749. DHX8.

HPA

HPA049285.

MIM

600396. gene.

neXtProt

NX_Q14562.

PharmGKB

PA27231.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1643.

HOGENOM

HOG000175261.

HOVERGEN

HBG039428.

InParanoid

Q14562.

KO

K12818.

OMA

NAWCYEN.

OrthoDB

EOG4D26P2.

PhylomeDB

Q14562.

Gene expression databases

ArrayExpress

Q14562.

Bgee

Q14562.

CleanEx

HS_DHX8.

Genevestigator

Q14562.

GermOnline

ENSG00000067596. Homo sapiens.

Family and domain databases

Gene3D

2.40.50.140. 1 hit.

InterPro

IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR022967. RNA-binding_domain_S1.
[Graphical view]

Pfam

PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

PROSITE

PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q14562.

GenomeRNAi

1659.

NextBio

6828.

SOURCE

Search...

Entry information

Entry name

DHX8_HUMAN

Accession

Primary (citable) accession number: Q14562

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.