Q14558 (KPRA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosyl pyrophosphate synthase-associated protein 1
Short name=PRPP synthase-associated protein 1

Alternative name(s):
39 kDa phosphoribosypyrophosphate synthase-associated protein
Short name=PAP39

Gene names

Name:

PRPSAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis.
Subunit structure	Binds to PRPS1 and PRPS2.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence BAG35521.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enzyme inhibitor activity Traceable author statement. Source: ProtInc magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q14558-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q14558-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPKKLLLLPPPSASSAFRVPRARPVPPPAM

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 356

356

Phosphoribosyl pyrophosphate synthase-associated protein 1

PRO_0000141079

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.7

Modified residue

215

1

Phosphoserine Ref.6

Natural variations

Alternative sequence

1

1

M → MPKKLLLLPPPSASSAFRVP RARPVPPPAM in isoform 2.

VSP_039062

Experimental info

Sequence conflict

12

1

S → L in BAA09612. Ref.1

Sequence conflict

54

1

S → F in BAA09612. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

356

Helix Strand Turn

Details...

Beta strand

9 – 12

4

Turn

17 – 20

4

Helix

21 – 29

9

Beta strand

37 – 41

5

Beta strand

47 – 51

5

Beta strand

60 – 63

4

Helix

70 – 86

17

Beta strand

92 – 98

7

Turn

100 – 105

6

Beta strand

109 – 111

3

Helix

114 – 124

11

Beta strand

129 – 134

6

Helix

138 – 143

6

Beta strand

148 – 151

4

Helix

154 – 164

11

Helix

168 – 170

3

Beta strand

172 – 177

6

Helix

178 – 180

3

Helix

181 – 191

11

Beta strand

194 – 198

5

Beta strand

243 – 245

3

Beta strand

250 – 255

6

Beta strand

257 – 261

5

Helix

263 – 273

11

Turn

274 – 276

3

Beta strand

277 – 287

11

Helix

293 – 299

7

Beta strand

304 – 308

5

Helix

314 – 319

6

Beta strand

323 – 326

4

Helix

329 – 341

13

Helix

346 – 349

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2002. Version 2. Checksum: 38CC87AB2C555717	FASTA	356	39,394
	10 20 30 40 50 60 MNAARTGYRV FSANSTAACT ELAKRITERL GAELGKSVVY QETNGETRVE IKESVRGQDI 70 80 90 100 110 120 FIIQTIPRDV NTAVMELLIM AYALKTACAR NIIGVIPYFP YSKQSKMRKR GSIVCKLLAS 130 140 150 160 170 180 MLAKAGLTHI ITMDLHQKEI QGFFSFPVDN LRASPFLLQY IQEEIPNYRN AVIVAKSPDA 190 200 210 220 230 240 AKRAQSYAER LRLGLAVIHG EAQCTELDMD DGRHSPPMVK NATVHPGLEL PLMMAKEKPP 250 260 270 280 290 300 ITVVGDVGGR IAIIVDDIID DVESFVAAAE ILKERGAYKI YVMATHGILS AEAPRLIEES 310 320 330 340 350 SVDEVVVTNT VPHEVQKLQC PKIKTVDISL ILSEAIRRIH NGESMAYLFR NITVDD
	Isoform 2 [UniParc]. Checksum: 3B9521CCB154F05D	FASTA	385	42,467
	10 20 30 40 50 60 MPKKLLLLPP PSASSAFRVP RARPVPPPAM NAARTGYRVF SANSTAACTE LAKRITERLG 70 80 90 100 110 120 AELGKSVVYQ ETNGETRVEI KESVRGQDIF IIQTIPRDVN TAVMELLIMA YALKTACARN 130 140 150 160 170 180 IIGVIPYFPY SKQSKMRKRG SIVCKLLASM LAKAGLTHII TMDLHQKEIQ GFFSFPVDNL 190 200 210 220 230 240 RASPFLLQYI QEEIPNYRNA VIVAKSPDAA KRAQSYAERL RLGLAVIHGE AQCTELDMDD 250 260 270 280 290 300 GRHSPPMVKN ATVHPGLELP LMMAKEKPPI TVVGDVGGRI AIIVDDIIDD VESFVAAAEI 310 320 330 340 350 360 LKERGAYKIY VMATHGILSA EAPRLIEESS VDEVVVTNTV PHEVQKLQCP KIKTVDISLI 370 380 LSEAIRRIHN GESMAYLFRN ITVDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of human complementary DNA for the phosphoribosylpyrophosphate synthetase-associated protein 39." Ishizuka T., Kita K., Sonoda T., Ishijima S., Sawa K., Suzuki N., Tatibana M. Biochim. Biophys. Acta 1306:27-30(1996) [PubMed: 8611620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hepatoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1/2). Tissue: Brain.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Crystal structure of human phosphoribosylpyrophosphate synthetase-associated protein 39 (Pap39)." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 5-361.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D61391 mRNA. Translation: BAA09612.1.
AK312637 mRNA. Translation: BAG35521.1. Different initiation.
AC090699 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89386.1.
BC009012 mRNA. Translation: AAH09012.1.

IPI

IPI00291578.
IPI00797603.

PIR

S71460.

RefSeq

NP_002757.2. NM_002766.2.

UniGene

Hs.77498.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C4K	X-ray	2.65	A/B/C/D/E/F	5-351	[»]

ProteinModelPortal

Q14558.

SMR

Q14558. Positions 7-350.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14558. 6 interactions.

MINT

MINT-1387668.

STRING

Q14558.

PTM databases

PhosphoSite

Q14558.

Polymorphism databases

DMDM

24418495.

Proteomic databases

PRIDE

Q14558.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000446526; ENSP00000414624; ENSG00000161542.
ENST00000448426; ENSP00000399690; ENSG00000161542.

GeneID

5635.

KEGG

hsa:5635.

Organism-specific databases

CTD

5635.

GeneCards

GC17M074306.

H-InvDB

HIX0014196.

HGNC

HGNC:9466. PRPSAP1.

MIM

601249. gene.

neXtProt

NX_Q14558.

PharmGKB

PA33821.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16660.

GeneTree

ENSGT00550000074583.

HOGENOM

HBG519284.

HOVERGEN

HBG001520.

InParanoid

Q14558.

OMA

CAKNIIG.

OrthoDB

EOG4C87SQ.

PhylomeDB

Q14558.

Gene expression databases

ArrayExpress

Q14558.

Bgee

Q14558.

CleanEx

HS_PRPSAP1.

Genevestigator

Q14558.

GermOnline

ENSG00000161542. Homo sapiens.

Family and domain databases

InterPro

IPR005946. Rib-P_diPkinase.
[Graphical view]

TIGRFAMs

TIGR01251. RibP_PPkin. 1 hit.

ProtoNet

Search...

Other

NextBio

21898.

SOURCE

Search...

Entry information

Entry name

KPRA_HUMAN

Accession

Primary (citable) accession number: Q14558
Secondary accession number(s): B2R6M4, Q96H06

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.