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Protein

Protein disulfide-isomerase A5

Gene

PDIA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

  • oxidoreductase activity Source: UniProtKB
  • protein disulfide isomerase activity Source: UniProtKB

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • IRE1-mediated unfolded protein response Source: Reactome
  • oxidation-reduction process Source: UniProtKB
  • protein folding Source: UniProtKB
  • response to endoplasmic reticulum stress Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciZFISH:HS00842-MONOMER.
ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A5 (EC:5.3.4.1)
Alternative name(s):
Protein disulfide isomerase-related protein
Gene namesi
Name:PDIA5
Synonyms:PDIR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24811. PDIA5.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  • endoplasmic reticulum lumen Source: UniProtKB
  • endoplasmic reticulum membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi10954.
OpenTargetsiENSG00000065485.
PharmGKBiPA142671191.

Polymorphism and mutation databases

BioMutaiPDIA5.
DMDMi2501208.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000003423322 – 519Protein disulfide-isomerase A5Add BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi182 ↔ 185Redox-activePROSITE-ProRule annotation
Disulfide bondi305 ↔ 308Redox-activePROSITE-ProRule annotation
Disulfide bondi426 ↔ 429Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ14554.
MaxQBiQ14554.
PaxDbiQ14554.
PeptideAtlasiQ14554.
PRIDEiQ14554.

PTM databases

iPTMnetiQ14554.
PhosphoSitePlusiQ14554.
SwissPalmiQ14554.

Expressioni

Gene expression databases

BgeeiENSG00000065485.
CleanExiHS_PDIA5.
ExpressionAtlasiQ14554. baseline and differential.
GenevisibleiQ14554. HS.

Organism-specific databases

HPAiHPA030353.
HPA030354.
HPA030355.
HPA030356.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-7P604093EBI-953879,EBI-10172290
MDFIQ997503EBI-953879,EBI-724076

Protein-protein interaction databases

BioGridi116154. 35 interactors.
IntActiQ14554. 14 interactors.
MINTiMINT-2868874.
STRINGi9606.ENSP00000323313.

Structurei

Secondary structure

1519
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 45Combined sources10
Beta strandi47 – 56Combined sources10
Helixi57 – 73Combined sources17
Turni74 – 77Combined sources4
Beta strandi79 – 84Combined sources6
Helixi88 – 96Combined sources9
Beta strandi108 – 114Combined sources7
Beta strandi117 – 121Combined sources5
Helixi128 – 136Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I6XX-ray1.50A29-150[»]
ProteinModelPortaliQ14554.
SMRiQ14554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 261Thioredoxin 1PROSITE-ProRule annotationAdd BLAST128
Domaini270 – 384Thioredoxin 2PROSITE-ProRule annotationAdd BLAST115
Domaini378 – 506Thioredoxin 3PROSITE-ProRule annotationAdd BLAST129

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi516 – 519Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 3 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133723.
HOGENOMiHOG000039967.
HOVERGENiHBG053547.
InParanoidiQ14554.
KOiK09583.
OMAiHKDYDRQ.
OrthoDBiEOG091G04ZS.
PhylomeDBiQ14554.
TreeFamiTF106379.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 3 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q14554-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAGPAWLL LAIWVVLPSW LSSAKVSSLI ERISDPKDLK KLLRTRNNVL
60 70 80 90 100
VLYSKSEVAA ENHLRLLSTV AQAVKGQGTI CWVDCGDAES RKLCKKMKVD
110 120 130 140 150
LSPKDKKVEL FHYQDGAFHT EYNRAVTFKS IVAFLKDPKG PPLWEEDPGA
160 170 180 190 200
KDVVHLDSEK DFRRLLKKEE KPLLIMFYAP WCSMCKRMMP HFQKAATQLR
210 220 230 240 250
GHAVLAGMNV YSSEFENIKE EYSVRGFPTI CYFEKGRFLF QYDNYGSTAE
260 270 280 290 300
DIVEWLKNPQ PPQPQVPETP WADEGGSVYH LTDEDFDQFV KEHSSVLVMF
310 320 330 340 350
HAPWCGHCKK MKPEFEKAAE ALHGEADSSG VLAAVDATVN KALAERFHIS
360 370 380 390 400
EFPTLKYFKN GEKYAVPVLR TKKKFLEWMQ NPEAPPPPEP TWEEQQTSVL
410 420 430 440 450
HLVGDNFRET LKKKKHTLVM FYAPWCPHCK KVIPHFTATA DAFKDDRKIA
460 470 480 490 500
CAAVDCVKDK NQDLCQQEAV KGYPTFHYYH YGKFAEKYDS DRTELGFTNY
510
IRALREGDHE RLGKKKEEL
Length:519
Mass (Da):59,594
Last modified:November 1, 1997 - v1
Checksum:i6083FBEB8C019658
GO
Isoform 2 (identifier: Q14554-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     258-261: NPQP → KVWP
     262-518: Missing.

Note: No experimental confirmation available.
Show »
Length:262
Mass (Da):30,124
Checksum:i5F1F4564D9DB3955
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052581391T → M.Corresponds to variant rs2292661dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056536258 – 261NPQP → KVWP in isoform 2. 1 Publication4
Alternative sequenceiVSP_056537262 – 518Missing in isoform 2. 1 PublicationAdd BLAST257

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49490 mRNA. Translation: BAA08451.1.
AC063922 Genomic DNA. No translation available.
AC083797 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79452.1.
CH471052 Genomic DNA. Translation: EAW79453.1.
CH471052 Genomic DNA. Translation: EAW79454.1.
CH471052 Genomic DNA. Translation: EAW79456.1.
BC001625 mRNA. Translation: AAH01625.1.
CCDSiCCDS3020.1. [Q14554-1]
PIRiS66673.
RefSeqiNP_006801.1. NM_006810.3. [Q14554-1]
UniGeneiHs.477352.

Genome annotation databases

EnsembliENST00000316218; ENSP00000323313; ENSG00000065485. [Q14554-1]
ENST00000489923; ENSP00000417520; ENSG00000065485. [Q14554-2]
GeneIDi10954.
KEGGihsa:10954.
UCSCiuc003egc.3. human. [Q14554-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49490 mRNA. Translation: BAA08451.1.
AC063922 Genomic DNA. No translation available.
AC083797 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79452.1.
CH471052 Genomic DNA. Translation: EAW79453.1.
CH471052 Genomic DNA. Translation: EAW79454.1.
CH471052 Genomic DNA. Translation: EAW79456.1.
BC001625 mRNA. Translation: AAH01625.1.
CCDSiCCDS3020.1. [Q14554-1]
PIRiS66673.
RefSeqiNP_006801.1. NM_006810.3. [Q14554-1]
UniGeneiHs.477352.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I6XX-ray1.50A29-150[»]
ProteinModelPortaliQ14554.
SMRiQ14554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116154. 35 interactors.
IntActiQ14554. 14 interactors.
MINTiMINT-2868874.
STRINGi9606.ENSP00000323313.

PTM databases

iPTMnetiQ14554.
PhosphoSitePlusiQ14554.
SwissPalmiQ14554.

Polymorphism and mutation databases

BioMutaiPDIA5.
DMDMi2501208.

Proteomic databases

EPDiQ14554.
MaxQBiQ14554.
PaxDbiQ14554.
PeptideAtlasiQ14554.
PRIDEiQ14554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316218; ENSP00000323313; ENSG00000065485. [Q14554-1]
ENST00000489923; ENSP00000417520; ENSG00000065485. [Q14554-2]
GeneIDi10954.
KEGGihsa:10954.
UCSCiuc003egc.3. human. [Q14554-1]

Organism-specific databases

CTDi10954.
DisGeNETi10954.
GeneCardsiPDIA5.
HGNCiHGNC:24811. PDIA5.
HPAiHPA030353.
HPA030354.
HPA030355.
HPA030356.
neXtProtiNX_Q14554.
OpenTargetsiENSG00000065485.
PharmGKBiPA142671191.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133723.
HOGENOMiHOG000039967.
HOVERGENiHBG053547.
InParanoidiQ14554.
KOiK09583.
OMAiHKDYDRQ.
OrthoDBiEOG091G04ZS.
PhylomeDBiQ14554.
TreeFamiTF106379.

Enzyme and pathway databases

BioCyciZFISH:HS00842-MONOMER.
ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.

Miscellaneous databases

ChiTaRSiPDIA5. human.
GenomeRNAii10954.
PROiQ14554.

Gene expression databases

BgeeiENSG00000065485.
CleanExiHS_PDIA5.
ExpressionAtlasiQ14554. baseline and differential.
GenevisibleiQ14554. HS.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 3 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA5_HUMAN
AccessioniPrimary (citable) accession number: Q14554
Secondary accession number(s): D3DN95, Q9BV43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.