true1997-11-012024-01-24193S29A2_HUMANA mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein.Williams J.B.Lanahan A.A.doi:10.1006/bbrc.1995.21331995Biochem. Biophys. Res. Commun.213325-333NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)HeartMolecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta.Griffiths M.Yao S.Y.Abidi F.Phillips S.E.Cass C.E.Young J.D.Baldwin S.A.doi:10.1042/bj32807391997Biochem. J.328739-743NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)FUNCTIONTRANSPORTER ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESMISCELLANEOUSPlacentaCloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transport-deficient cell line.Crawford C.R.Patel D.H.Naeve C.Belt J.A.doi:10.1074/jbc.273.9.52881998J. Biol. Chem.2735288-5293NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)FUNCTIONTRANSPORTER ACTIVITYTISSUE SPECIFICITYMISCELLANEOUSLocalization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells.Mangravite L.M.Xiao G.Giacomini K.M.doi:10.1152/ajprenal.00215.20022003Am. J. Physiol.284F902-F910NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3)FUNCTIONTRANSPORTER ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESSUBCELLULAR LOCATIONMUTAGENESIS OF LEU-455 AND LEU-456Complete sequencing and characterization of 21,243 full-length human cDNAs.Ota T.Suzuki Y.Nishikawa T.Otsuki T.Sugiyama T.Irie R.Wakamatsu A.Hayashi K.Sato H.Nagai K.Kimura K.Makita H.Sekine M.Obayashi M.Nishi T.Shibahara T.Tanaka T.Ishii S.Yamamoto J.Saito K.Kawai Y.Isono Y.Nakamura Y.Nagahari K.Murakami K.Yasuda T.Iwayanagi T.Wagatsuma M.Shiratori A.Sudo H.Hosoiri T.Kaku Y.Kodaira H.Kondo H.Sugawara M.Takahashi M.Kanda K.Yokoi T.Furuya T.Kikkawa E.Omura Y.Abe K.Kamihara K.Katsuta N.Sato K.Tanikawa M.Yamazaki M.Ninomiya K.Ishibashi T.Yamashita H.Murakawa K.Fujimori K.Tanai H.Kimata M.Watanabe M.Hiraoka S.Chiba Y.Ishida S.Ono Y.Takiguchi S.Watanabe S.Yosida M.Hotuta T.Kusano J.Kanehori K.Takahashi-Fujii A.Hara H.Tanase T.-O.Nomura Y.Togiya S.Komai F.Hara R.Takeuchi K.Arita M.Imose N.Musashino K.Yuuki H.Oshima A.Sasaki N.Aotsuka S.Yoshikawa Y.Matsunawa H.Ichihara T.Shiohata N.Sano S.Moriya S.Momiyama H.Satoh N.Takami S.Terashima Y.Suzuki O.Nakagawa S.Senoh A.Mizoguchi H.Goto Y.Shimizu F.Wakebe H.Hishigaki H.Watanabe T.Sugiyama A.Takemoto M.Kawakami B.Yamazaki M.'Watanabe K.Kumagai A.Itakura S.Fukuzumi Y.Fujimori Y.Komiyama M.Tashiro H.Tanigami A.Fujiwara T.Ono T.Yamada K.Fujii Y.Ozaki K.Hirao M.Ohmori Y.Kawabata A.Hikiji T.Kobatake N.Inagaki H.Ikema Y.Okamoto S.Okitani R.Kawakami T.Noguchi S.Itoh T.Shigeta K.Senba T.Matsumura K.Nakajima Y.Mizuno T.Morinaga M.Sasaki M.Togashi T.Oyama M.Hata H.Watanabe M.'Komatsu T.Mizushima-Sugano J.Satoh T.Shirai Y.Takahashi Y.Nakagawa K.Okumura K.Nagase T.Nomura N.Kikuchi H.Masuho Y.Yamashita R.Nakai K.Yada T.Nakamura Y.'Ohara O.Isogai T.Sugano S.doi:10.1038/ng12852004Nat. Genet.3640-45NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)Human chromosome 11 DNA sequence and analysis including novel gene identification.Taylor T.D.Noguchi H.Totoki Y.Toyoda A.Kuroki Y.Dewar K.Lloyd C.Itoh T.Takeda T.Kim D.-W.She X.Barlow K.F.Bloom T.Bruford E.Chang J.L.Cuomo C.A.Eichler E.FitzGerald M.G.Jaffe D.B.LaButti K.Nicol R.Park H.-S.Seaman C.Sougnez C.Yang X.Zimmer A.R.Zody M.C.Birren B.W.Nusbaum C.Fujiyama A.Hattori M.Rogers J.Lander E.S.Sakaki Y.doi:10.1038/nature046322006Nature440497-500NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Mural R.J.Istrail S.Sutton G.G.Florea L.Halpern A.L.Mobarry C.M.Lippert R.Walenz B.Shatkay H.Dew I.Miller J.R.Flanigan M.J.Edwards N.J.Bolanos R.Fasulo D.Halldorsson B.V.Hannenhalli S.Turner R.Yooseph S.Lu F.Nusskern D.R.Shue B.C.Zheng X.H.Zhong F.Delcher A.L.Huson D.H.Kravitz S.A.Mouchard L.Reinert K.Remington K.A.Clark A.G.Waterman M.S.Eichler E.E.Adams M.D.Hunkapiller M.W.Myers E.W.Venter J.C.2005-07EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).The MGC Project Teamdoi:10.1101/gr.25965042004Genome Res.142121-2127NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]LiverKinetic and pharmacological properties of cloned human equilibrative nucleoside transporters, ENT1 and ENT2, stably expressed in nucleoside transporter-deficient PK15 cells. Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine.Ward J.L.Sherali A.Mo Z.P.Tse C.M.doi:10.1074/jbc.275.12.83752000J. Biol. Chem.2758375-8381FUNCTIONTRANSPORTER ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESGLYCOSYLATIONMISCELLANEOUSFunctional analysis of site-directed glycosylation mutants of the human equilibrative nucleoside transporter-2.Ward J.L.Leung G.P.Toan S.V.Tse C.-M.doi:10.1016/s0003-9861(02)00718-x2003Arch. Biochem. Biophys.41119-26FUNCTIONTRANSPORTER ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESGLYCOSYLATION AT ASN-48 AND ASN-57MUTAGENESIS OF ASN-48 AND ASN-57MISCELLANEOUSLys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.Gauci S.Helbig A.O.Slijper M.Krijgsveld J.Heck A.J.Mohammed S.doi:10.1021/ac90043092009Anal. Chem.814493-4501IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.Olsen J.V.Vermeulen M.Santamaria A.Kumar C.Miller M.L.Jensen L.J.Gnad F.Cox J.Jensen T.S.Nigg E.A.Brunak S.Mann M.doi:10.1126/scisignal.20004752010Sci. Signal.3RA3PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Cervix carcinomaNucleobase transport by human equilibrative nucleoside transporter 1 (hENT1).Yao S.Y.Ng A.M.Cass C.E.Baldwin S.A.Young J.D.doi:10.1074/jbc.m111.2361172011J. Biol. Chem.28632552-32562FUNCTIONTRANSPORTER ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESMISCELLANEOUSSystem-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.Rigbolt K.T.Prokhorova T.A.Akimov V.Henningsen J.Johansen P.T.Kratchmarova I.Kassem M.Mann M.Olsen J.V.Blagoev B.doi:10.1126/scisignal.20015702011Sci. Signal.4RS3PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Basolateral uptake of nucleosides by Sertoli cells is mediated primarily by equilibrative nucleoside transporter 1.Klein D.M.Evans K.K.Hardwick R.N.Dantzler W.H.Wright S.H.Cherrington N.J.doi:10.1124/jpet.113.2032652013J. Pharmacol. Exp. Ther.346121-129FUNCTIONSUBCELLULAR LOCATIONTISSUE SPECIFICITYToward a comprehensive characterization of a human cancer cell phosphoproteome.Zhou H.Di Palma S.Preisinger C.Peng M.Polat A.N.Heck A.J.Mohammed S.doi:10.1021/pr300630k2013J. Proteome Res.12260-271IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]ErythroleukemiaNovel nuclear hENT2 isoforms regulate cell cycle progression via controlling nucleoside transport and nuclear reservoir.Grane-Boladeras N.Spring C.M.Hanna W.J.Pastor-Anglada M.Coe I.R.doi:10.1007/s00018-016-2288-92016Cell. Mol. Life Sci.734559-4575CAUTIONFunctional characterization and haplotype analysis of polymorphisms in the human equilibrative nucleoside transporter, ENT2.Owen R.P.Lagpacan L.L.Taylor T.R.De La Cruz M.Huang C.C.Kawamoto M.Johns S.J.Stryke D.Ferrin T.E.Giacomini K.M.doi:10.1124/dmd.105.0062702006Drug Metab. Dispos.3412-15FUNCTIONTRANSPORTER ACTIVITYVARIANTS TYR-5; LYS-68; LEU-94 AND 184-SER--VAL-186 DELINS METCHARACTERIZATION OF VARIANTS TYR-5; LYS-68; LEU-94 AND 184-SER--VAL-186 DELINS METSequence problems.262AdenosineAzathioprineDidanosineEthanolGemcitabineMercaptopurineTegafur-uracilTrifluridineZalcitabineZidovudinethe equilibrative nucleoside transporter (ent) family3 sites, No reported glycans3 sites175 antibodies from 23 providershumanSLC29A2Group enriched (skeletal muscle, tongue)geneEukaryotaTransport of nucleosides and free purine and pyrimidine bases across the plasma membraneAzathioprine ADME16 hits in 1155 CRISPR screenshumanTbioProteinExpressed in gastrocnemius and 149 other cell types or tissuesbaseline and differentialMFS general substrate transporter like domainsENT1/ENT2Eqnu_transptMFS_trans_sf2a57EQUILIBRATIVE NUCLEOSIDE TRANSPORTEREQUILIBRATIVE NUCLEOSIDE TRANSPORTER 2Nucleoside_tranENTDERENTRNSPRTMFS general substrate transporterHSEquilibrative nucleoside transporter 2hENT236 kDa nucleolar protein HNP36Delayed-early response protein 12Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporterEquilibrative NBMPR-insensitive nucleoside transporterHydrophobic nucleolar protein, 36 kDaNucleoside transporter, ei-typeSolute carrier family 29 member 2SLC29A2DER12ENT2HNP36Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed:9396714, PubMed:9478986, PubMed:12527552, PubMed:10722669, PubMed:12590919, PubMed:21795683, PubMed:16214850). Functions as a Na(+)-independent, passive transporter (PubMed:9478986). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine (PubMed:9396714, PubMed:9478986, PubMed:12527552, PubMed:10722669, PubMed:12590919, PubMed:21795683, PubMed:16214850). Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed:21795683, PubMed:16214850). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (PubMed:12527552). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier (PubMed:23639800). Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity).Non functional nucleoside transporter protein for adenosine or thymidine transport. Does not express on cell membrane.50 uM for inosine75.9 uM for adenosine140 uM for adenosine200 uM for uridine250 uM for uridine330 uM for uridine500 uM for uridine710 uM for thymidine1500 uM for hypoxanthine1800 uM for adenine3900 uM for cytidine5610 uM for cytidine6000 uM for thymine72 pmol/min/mg enzyme for inosine uptake1147 pmol/min/mg enzyme for adenosine uptake2055 pmol/min/mg enzyme for uridine uptake2486 pmol/min/mg enzyme for thymidine uptake9740 pmol/min/mg enzyme for cytidine uptakeLocalized to the apical membrane of Sertoli cells.Highly expressed in skeletal muscle (PubMed:9478986). Expressed in liver, lung, placenta, brain, heart, kidney and ovarian tissues (PubMed:9478986). Expressed in testis at the blood-brain-barrier (PubMed:23639800).Glycosylated.Transport activity is insensitive to nanomolar concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) (PubMed:9396714, PubMed:9478986, PubMed:10722669, PubMed:12590919, PubMed:21795683). Inhibited by higher concentrations of NBMPR (1uM-10uM) (PubMed:9396714, PubMed:9478986, PubMed:10722669, PubMed:12590919).May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.The nuclear function of SLC29A2/ENT2 is unclear. A study reported that under proliferative conditions, two SLC29A2/ENT2 nuclear isoforms recruited SLC29A2/ENT2 to the nuclear envelope in order to translocate nucleosides into the nucleus for incorporation into DNA during replication (PubMed:27271752). However, the physiological existence of these isoforms has yet to be proven.Wrong choice of CDS.Equilibrative nucleoside transporter 2501131456Helical1333Helical7090Helical99119Helical124144Helical162182Helical193213Helical291311Helical324344Helical360380Helical386406Helical432452Phosphoserine252N-linked (GlcNAc...) asparagine48N-linked (GlcNAc...) asparagine57N-linked (GlcNAc...) asparagine225In isoform 2.AGQGGHEPRTLLLHHHGLRLLHQLLQCSPTGQPLRAAGHHALHLQHPLPQRPGPGWDLCCPCHAPVHGQWRGRRDLCPGVLYHALCGHPHVHRVLPEPASPEVCPLLPGQ93202In isoform 2.203In isoform 4.LADSAVPCVGLHSHPVRLPRHHSHGDQLHQSWEVESVLQPHLLLPPLQHHGLAGTEPDLLLPVARRGQPAAAPAGLPAVPVRAPLHAVPRAPEVPAAHPLPTGCLLHHLHAALCRF246In isoform 3.SPCPSSPPSQPW290301In isoform 3.302Decreased nucleoside and nucleobase transport.Y5No change in nucleoside and nucleobase transport.K68No change in nucleoside and nucleobase transport.L94Decreased inosine and guanosine transport; no change in uridine and hypoxanthine transport.M184186No difference in uridine or cytidine uptake. No differences in Km values and lower Vmax values for either uridine or cytidine uptake; when associated with D-57.DNo difference in uridine or cytidine uptake. No differences in Km values and lower Vmax values for either uridine or cytidine uptake; when associated with D-48.DReduces drastically localization at the cell surface. No effect on uptake of adenosine and thymidine. Reduces drastically localization at the cell surface and induces an significant reduction of adenosine or thymidine uptake; when associated with R-456.R455Reduces drastically localization at the cell surface and induces an significant reduction of adenosine or thymidine uptake; when associated with R-455.RY200Y2006-10-17350113a35574e4a459a088da36a4ec54438cbe1LongMARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNNWVTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVDMSPGPFFSITMASVCFINSFSAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLLSMASGVDAETSALGYFITPCVGILMSIVCYLSLPHLKFARYYLANKSSQAQAQELETKAELLQSDENGIPSSPQKVALTLDLDLEKEPESEPDEPQKPGKPSVFTVFQKIWLTALCLVLVFTVTLSVFPAITAMVTSSTSPGKWSQFFNPICCFLLFNIMDWLGRSLTSYFLWPDEDSRLLPLLVCLRFLFVPLFMLCHVPQRSRLPILFPQDAYFITFMLLFAVSNGYLVSLTMCLAPRQVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL2ShortHNP36MARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNNWVTLLSQLPLLLFTLLNSFLYQCAGQGGHEPRTLLLHHHGLRLLHQLLQCSPTGQPLRAAGHHALHLQHPLPQRPGPGWDLCCPCHAPVHGQWRGRRDLCPGVLYHALCGHPHVHRVLPEPASPEVCPLLPGQ3hENT2AMARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNNWVTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVDMSPGPFFSITMASVCFINSFSAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLLSMASGVDAETSALGYFITPCVGILMSIVCYLSLPHLKFARYYLANKSSQAQAQELETKAELLQSDENGIPSSPQKVALTLDLDLEKEPESEPDEPQKPGKPSVFTVFQKSPCPSSPPSQPW4MARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNNWVTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVDMSPGPFFSITMASVCFINSFSAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLLSMASGVDAETSALGYFITPCVGILMSIVCYLSLPHLKFARYYLANKSSQAQAQELETKAELLQSDLADSAVPCVGLHSHPVRLPRHHSHGDQLHQSWEVESVLQPHLLLPPLQHHGLAGTEPDLLLPVARRGQPAAAPAGLPAVPVRAPLHAVPRAPEVPAAHPLPTGCLLHHLHAALCRFtruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetrue