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Protein

Equilibrative nucleoside transporter 2

Gene

SLC29A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine.1 Publication

Caution

Kineticsi

Vmax for adenosine uptake is about the same for SLC29A1 and SLC29A2.
  1. KM=0.2 mM for uridine2 Publications
  2. KM=0.75 mM for adenosine2 Publications

    GO - Molecular functioni

    GO - Biological processi

    • cell proliferation Source: ProtInc
    • nucleobase-containing compound metabolic process Source: ProtInc
    • nucleoside transport Source: UniProtKB

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    ReactomeiR-HSA-83936 Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane

    Protein family/group databases

    TCDBi2.A.57.1.8 the equilibrative nucleoside transporter (ent) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Equilibrative nucleoside transporter 2
    Alternative name(s):
    36 kDa nucleolar protein HNP36
    Delayed-early response protein 12
    Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter
    Short name:
    Equilibrative NBMPR-insensitive nucleoside transporter
    Hydrophobic nucleolar protein, 36 kDa
    Nucleoside transporter, ei-type
    Solute carrier family 29 member 2
    Gene namesi
    Name:SLC29A2
    Synonyms:DER12, ENT2, HNP36
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 11

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000174669.11
    HGNCiHGNC:11004 SLC29A2
    MIMi602110 gene
    neXtProtiNX_Q14542

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
    Transmembranei70 – 90HelicalSequence analysisAdd BLAST21
    Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
    Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
    Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
    Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
    Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
    Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
    Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
    Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
    Transmembranei432 – 452HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi455L → R: Reduces drastically localization at the cell surface. No effect on uptake of adenosine and thymidine. Reduces drastically localization at the cell surface and induces an significant reduction of adenosine or thymidine uptake; when associated with R-456. 1 Publication1
    Mutagenesisi456L → R: Reduces drastically localization at the cell surface and induces an significant reduction of adenosine or thymidine uptake; when associated with R-455. 1 Publication1

    Organism-specific databases

    DisGeNETi3177
    OpenTargetsiENSG00000174669
    PharmGKBiPA191

    Chemistry databases

    ChEMBLiCHEMBL3509606
    DrugBankiDB00900 Didanosine
    DB00898 Ethanol
    DB00441 Gemcitabine
    DB01033 Mercaptopurine
    DB00943 Zalcitabine
    DB00495 Zidovudine

    Polymorphism and mutation databases

    BioMutaiSLC29A2
    DMDMi116242781

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002093401 – 456Equilibrative nucleoside transporter 2Add BLAST456

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi48N-linked (GlcNAc...) asparagine1 Publication1
    Glycosylationi57N-linked (GlcNAc...) asparagine1 Publication1
    Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1
    Modified residuei252PhosphoserineCombined sources1

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    EPDiQ14542
    MaxQBiQ14542
    PaxDbiQ14542
    PeptideAtlasiQ14542
    PRIDEiQ14542

    PTM databases

    iPTMnetiQ14542
    PhosphoSitePlusiQ14542

    Expressioni

    Tissue specificityi

    Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues.2 Publications

    Inductioni

    By PDGF/platelet derived growth factor and fibroblast growth factor (FGF).

    Gene expression databases

    BgeeiENSG00000174669
    CleanExiHS_SLC29A2
    ExpressionAtlasiQ14542 baseline and differential
    GenevisibleiQ14542 HS

    Organism-specific databases

    HPAiHPA018168

    Interactioni

    Protein-protein interaction databases

    BioGridi1094195 interactors.
    IntActiQ14542 1 interactor.
    MINTiQ14542
    STRINGi9606.ENSP00000350024

    Chemistry databases

    BindingDBiQ14542

    Structurei

    3D structure databases

    ProteinModelPortaliQ14542
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1479 Eukaryota
    ENOG410Y3MT LUCA
    GeneTreeiENSGT00390000002232
    HOGENOMiHOG000007684
    HOVERGENiHBG074626
    InParanoidiQ14542
    KOiK15014
    OMAiVWFINSF
    OrthoDBiEOG091G09WB
    PhylomeDBiQ14542
    TreeFamiTF313950

    Family and domain databases

    InterProiView protein in InterPro
    IPR034764 ENT1/ENT2
    IPR030197 ENT2
    IPR002259 Eqnu_transpt
    IPR036259 MFS_trans_sf
    PANTHERiPTHR10332 PTHR10332, 1 hit
    PTHR10332:SF8 PTHR10332:SF8, 1 hit
    PfamiView protein in Pfam
    PF01733 Nucleoside_tran, 1 hit
    PIRSFiPIRSF016379 ENT, 1 hit
    PRINTSiPR01130 DERENTRNSPRT
    SUPFAMiSSF103473 SSF103473, 2 hits
    TIGRFAMsiTIGR00939 2a57, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q14542-1) [UniParc]FASTAAdd to basket
    Also known as: Long

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MARGDAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ ARLAGAGNST
    60 70 80 90 100
    ARILSTNHTG PEDAFNFNNW VTLLSQLPLL LFTLLNSFLY QCVPETVRIL
    110 120 130 140 150
    GSLLAILLLF ALTAALVKVD MSPGPFFSIT MASVCFINSF SAVLQGSLFG
    160 170 180 190 200
    QLGTMPSTYS TLFLSGQGLA GIFAALAMLL SMASGVDAET SALGYFITPC
    210 220 230 240 250
    VGILMSIVCY LSLPHLKFAR YYLANKSSQA QAQELETKAE LLQSDENGIP
    260 270 280 290 300
    SSPQKVALTL DLDLEKEPES EPDEPQKPGK PSVFTVFQKI WLTALCLVLV
    310 320 330 340 350
    FTVTLSVFPA ITAMVTSSTS PGKWSQFFNP ICCFLLFNIM DWLGRSLTSY
    360 370 380 390 400
    FLWPDEDSRL LPLLVCLRFL FVPLFMLCHV PQRSRLPILF PQDAYFITFM
    410 420 430 440 450
    LLFAVSNGYL VSLTMCLAPR QVLPHEREVA GALMTFFLAL GLSCGASLSF

    LFKALL
    Length:456
    Mass (Da):50,113
    Last modified:October 17, 2006 - v3
    Checksum:iABCBD244306708E1
    GO
    Isoform 2 (identifier: Q14542-2) [UniParc]FASTAAdd to basket
    Also known as: Short, HNP36

    The sequence of this isoform differs from the canonical sequence as follows:
         93-202: VPETVRILGS...LGYFITPCVG → AGQGGHEPRT...PEVCPLLPGQ
         203-456: Missing.

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
    Show »
    Length:202
    Mass (Da):22,276
    Checksum:iE1E980B5CCC29586
    GO
    Isoform 3 (identifier: Q14542-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         290-301: IWLTALCLVLVF → SPCPSSPPSQPW
         302-456: Missing.

    Show »
    Length:301
    Mass (Da):32,507
    Checksum:i5DFD77C4226D4F15
    GO

    Sequence cautioni

    The sequence CAA60380 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti200C → Y in CAA60380 (PubMed:7639753).Curated1
    Sequence conflicti200C → Y in AAC39526 (PubMed:9396714).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_0292895D → Y1 PublicationCorresponds to variant dbSNP:rs8187643Ensembl.1
    Natural variantiVAR_02929068N → K1 PublicationCorresponds to variant dbSNP:rs8187644Ensembl.1
    Natural variantiVAR_02929194P → L1 PublicationCorresponds to variant dbSNP:rs8187648Ensembl.1
    Natural variantiVAR_036822184 – 186SGV → M1 Publication3

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_04072893 – 202VPETV…TPCVG → AGQGGHEPRTLLLHHHGLRL LHQLLQCSPTGQPLRAAGHH ALHLQHPLPQRPGPGWDLCC PCHAPVHGQWRGRRDLCPGV LYHALCGHPHVHRVLPEPAS PEVCPLLPGQ in isoform 2. 1 PublicationAdd BLAST110
    Alternative sequenceiVSP_040729203 – 456Missing in isoform 2. 1 PublicationAdd BLAST254
    Alternative sequenceiVSP_040730290 – 301IWLTA…LVLVF → SPCPSSPPSQPW in isoform 3. 1 PublicationAdd BLAST12
    Alternative sequenceiVSP_040731302 – 456Missing in isoform 3. 1 PublicationAdd BLAST155

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X86681 mRNA Translation: CAA60380.1 Sequence problems.
    AF029358 mRNA Translation: AAC39526.1
    AF034102 mRNA Translation: AAB97834.1
    AK057041 mRNA Translation: BAG51849.1
    AF401235 mRNA Translation: AAK92533.1
    AP001107 Genomic DNA No translation available.
    CH471076 Genomic DNA Translation: EAW74521.1
    CH471076 Genomic DNA Translation: EAW74519.1
    BC093634 mRNA Translation: AAH93634.1
    CCDSiCCDS8137.1 [Q14542-1]
    PIRiJC4196
    RefSeqiNP_001287797.1, NM_001300868.1 [Q14542-1]
    NP_001523.2, NM_001532.2 [Q14542-1]
    XP_016873121.1, XM_017017632.1 [Q14542-1]
    UniGeneiHs.569017
    Hs.736345

    Genome annotation databases

    EnsembliENST00000357440; ENSP00000350024; ENSG00000174669 [Q14542-1]
    ENST00000540386; ENSP00000444870; ENSG00000174669 [Q14542-3]
    ENST00000541567; ENSP00000442116; ENSG00000174669 [Q14542-2]
    ENST00000544554; ENSP00000439456; ENSG00000174669 [Q14542-1]
    ENST00000546034; ENSP00000440329; ENSG00000174669 [Q14542-1]
    GeneIDi3177
    KEGGihsa:3177
    UCSCiuc001oht.4 human [Q14542-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiS29A2_HUMAN
    AccessioniPrimary (citable) accession number: Q14542
    Secondary accession number(s): B3KPY7
    , O43530, Q52M84, Q96R00, Q9UPE0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 17, 2006
    Last modified: February 28, 2018
    This is version 159 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome