Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q14534

- ERG1_HUMAN

UniProt

Q14534 - ERG1_HUMAN

Protein

Squalene monooxygenase

Gene

SQLE

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

    Catalytic activityi

    Squalene + NADPH + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + NADP+ + H2O.

    Cofactori

    FAD.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi126 – 15328FADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. squalene monooxygenase activity Source: UniProtKB

    GO - Biological processi

    1. cellular aromatic compound metabolic process Source: Ensembl
    2. cholesterol biosynthetic process Source: Reactome
    3. response to organic substance Source: Ensembl
    4. small molecule metabolic process Source: Reactome
    5. sterol biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:HS02595-MONOMER.
    ReactomeiREACT_147904. Activation of gene expression by SREBF (SREBP).
    REACT_9405. Cholesterol biosynthesis.
    SABIO-RKQ14534.
    UniPathwayiUPA00767; UER00752.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Squalene monooxygenase (EC:1.14.13.132)
    Alternative name(s):
    Squalene epoxidase
    Short name:
    SE
    Gene namesi
    Name:SQLE
    Synonyms:ERG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:11279. SQLE.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36108.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Squalene monooxygenasePRO_0000209838Add
    BLAST

    Proteomic databases

    MaxQBiQ14534.
    PaxDbiQ14534.
    PRIDEiQ14534.

    PTM databases

    PhosphoSiteiQ14534.

    Expressioni

    Gene expression databases

    ArrayExpressiQ14534.
    BgeeiQ14534.
    CleanExiHS_SQLE.
    GenevestigatoriQ14534.

    Organism-specific databases

    HPAiHPA018038.
    HPA020762.

    Interactioni

    Subunit structurei

    May form a complex with squalene synthase.

    Protein-protein interaction databases

    BioGridi112591. 5 interactions.
    IntActiQ14534. 3 interactions.
    STRINGi9606.ENSP00000265896.

    Structurei

    3D structure databases

    ProteinModelPortaliQ14534.
    SMRiQ14534. Positions 116-182.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei61 – 8121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei546 – 56621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the squalene monooxygenase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0654.
    HOGENOMiHOG000174713.
    HOVERGENiHBG005603.
    InParanoidiQ14534.
    KOiK00511.
    OMAiFITLANR.
    OrthoDBiEOG7J446G.
    PhylomeDBiQ14534.
    TreeFamiTF331056.

    Family and domain databases

    InterProiIPR001327. Pyr_OxRdtase_NAD-bd_dom.
    IPR003042. Rng_hydrolase-like.
    IPR013698. Squalene_epoxidase.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF08491. SE. 1 hit.
    [Graphical view]
    PRINTSiPR00420. RNGMNOXGNASE.

    Sequencei

    Sequence statusi: Complete.

    Q14534-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWTFLGIATF TYFYKKFGDF ITLANREVLL CVLVFLSLGL VLSYRCRHRN    50
    GGLLGRQQSG SQFALFSDIL SGLPFIGFFW AKSPPESENK EQLEARRRRK 100
    GTNISETSLI GTAACTSTSS QNDPEVIIVG AGVLGSALAA VLSRDGRKVT 150
    VIERDLKEPD RIVGEFLQPG GYHVLKDLGL GDTVEGLDAQ VVNGYMIHDQ 200
    ESKSEVQIPY PLSENNQVQS GRAFHHGRFI MSLRKAAMAE PNAKFIEGVV 250
    LQLLEEDDVV MGVQYKDKET GDIKELHAPL TVVADGLFSK FRKSLVSNKV 300
    SVSSHFVGFL MKNAPQFKAN HAELILANPS PVLIYQISSS ETRVLVDIRG 350
    EMPRNLREYM VEKIYPQIPD HLKEPFLEAT DNSHLRSMPA SFLPPSSVKK 400
    RGVLLLGDAY NMRHPLTGGG MTVAFKDIKL WRKLLKGIPD LYDDAAIFEA 450
    KKSFYWARKT SHSFVVNILA QALYELFSAT DDSLHQLRKA CFLYFKLGGE 500
    CVAGPVGLLS VLSPNPLVLI GHFFAVAIYA VYFCFKSEPW ITKPRALLSS 550
    GAVLYKACSV IFPLIYSEMK YMVH 574
    Length:574
    Mass (Da):63,923
    Last modified:May 18, 2010 - v3
    Checksum:i6957F5CF1F1624C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti43 – 431S → F in BX647400. (PubMed:17974005)Curated
    Sequence conflicti58 – 581Q → R in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti94 – 941E → G in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti139 – 1391A → V in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti247 – 2471E → G in BAA11209. (PubMed:8626488)Curated
    Sequence conflicti334 – 3341I → T in BX647400. (PubMed:17974005)Curated
    Sequence conflicti336 – 3361Q → R in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti379 – 3802AT → VA in BX647400. (PubMed:17974005)Curated
    Sequence conflicti389 – 3891P → L in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti451 – 4511K → N in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti518 – 5181V → A in BAA22372. (PubMed:9286711)Curated
    Sequence conflicti551 – 5511G → S in BAA22372. (PubMed:9286711)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78130 mRNA. Translation: BAA22372.1.
    BX647400 mRNA. No translation available.
    AC009908 Genomic DNA. No translation available.
    FJ695197 Genomic DNA. No translation available.
    D78129 mRNA. Translation: BAA11209.1.
    CCDSiCCDS47918.1.
    RefSeqiNP_003120.2. NM_003129.3.
    UniGeneiHs.71465.

    Genome annotation databases

    EnsembliENST00000265896; ENSP00000265896; ENSG00000104549.
    GeneIDi6713.
    KEGGihsa:6713.
    UCSCiuc011liq.2. human.

    Polymorphism databases

    DMDMi296439362.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78130 mRNA. Translation: BAA22372.1 .
    BX647400 mRNA. No translation available.
    AC009908 Genomic DNA. No translation available.
    FJ695197 Genomic DNA. No translation available.
    D78129 mRNA. Translation: BAA11209.1 .
    CCDSi CCDS47918.1.
    RefSeqi NP_003120.2. NM_003129.3.
    UniGenei Hs.71465.

    3D structure databases

    ProteinModelPortali Q14534.
    SMRi Q14534. Positions 116-182.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112591. 5 interactions.
    IntActi Q14534. 3 interactions.
    STRINGi 9606.ENSP00000265896.

    Chemistry

    ChEMBLi CHEMBL3592.
    DrugBanki DB01091. Butenafine.
    DB00735. Naftifine.
    DB00857. Terbinafine.

    PTM databases

    PhosphoSitei Q14534.

    Polymorphism databases

    DMDMi 296439362.

    Proteomic databases

    MaxQBi Q14534.
    PaxDbi Q14534.
    PRIDEi Q14534.

    Protocols and materials databases

    DNASUi 6713.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000265896 ; ENSP00000265896 ; ENSG00000104549 .
    GeneIDi 6713.
    KEGGi hsa:6713.
    UCSCi uc011liq.2. human.

    Organism-specific databases

    CTDi 6713.
    GeneCardsi GC08P126010.
    H-InvDB HIX0007779.
    HGNCi HGNC:11279. SQLE.
    HPAi HPA018038.
    HPA020762.
    MIMi 602019. gene.
    neXtProti NX_Q14534.
    PharmGKBi PA36108.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0654.
    HOGENOMi HOG000174713.
    HOVERGENi HBG005603.
    InParanoidi Q14534.
    KOi K00511.
    OMAi FITLANR.
    OrthoDBi EOG7J446G.
    PhylomeDBi Q14534.
    TreeFami TF331056.

    Enzyme and pathway databases

    UniPathwayi UPA00767 ; UER00752 .
    BioCyci MetaCyc:HS02595-MONOMER.
    Reactomei REACT_147904. Activation of gene expression by SREBF (SREBP).
    REACT_9405. Cholesterol biosynthesis.
    SABIO-RK Q14534.

    Miscellaneous databases

    GeneWikii Squalene_monooxygenase.
    GenomeRNAii 6713.
    NextBioi 26182.
    PROi Q14534.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q14534.
    Bgeei Q14534.
    CleanExi HS_SQLE.
    Genevestigatori Q14534.

    Family and domain databases

    InterProi IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    IPR003042. Rng_hydrolase-like.
    IPR013698. Squalene_epoxidase.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF08491. SE. 1 hit.
    [Graphical view ]
    PRINTSi PR00420. RNGMNOXGNASE.
    ProtoNeti Search...

    Publicationsi

    1. "Localization of the squalene epoxidase gene (SQLE) to human chromosome region 8q24.1."
      Nagai M., Sakakibara J., Wakui K., Fukushima Y., Igarashi S., Tsuji S., Arakawa M., Ono T.
      Genomics 44:141-143(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon endothelium.
    3. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Transcriptional regulation of squalene epoxidase by sterols and inhibitors in HeLa cells."
      Nakamura Y., Sakakibara J., Izumi T., Shibata A., Ono T.
      J. Biol. Chem. 271:8053-8056(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 187-535.
      Tissue: Liver.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiERG1_HUMAN
    AccessioniPrimary (citable) accession number: Q14534
    Secondary accession number(s): Q9UEK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3