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Q14534 (ERG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Squalene monooxygenase

EC=1.14.13.132
Alternative name(s):
Squalene epoxidase
Short name=SE
Gene names
Name:SQLE
Synonyms:ERG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Catalytic activity

Squalene + NADPH + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + NADP+ + H2O.

Cofactor

FAD.

Pathway

Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.

Subunit structure

May form a complex with squalene synthase.

Subcellular location

Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the squalene monooxygenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Squalene monooxygenase
PRO_0000209838

Regions

Transmembrane20 – 4021Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane546 – 56621Helical; Potential
Nucleotide binding126 – 15328FAD Potential

Experimental info

Sequence conflict431S → F in BX647400. Ref.2
Sequence conflict581Q → R in BAA22372. Ref.1
Sequence conflict941E → G in BAA22372. Ref.1
Sequence conflict1391A → V in BAA22372. Ref.1
Sequence conflict2471E → G in BAA11209. Ref.4
Sequence conflict3341I → T in BX647400. Ref.2
Sequence conflict3361Q → R in BAA22372. Ref.1
Sequence conflict379 – 3802AT → VA in BX647400. Ref.2
Sequence conflict3891P → L in BAA22372. Ref.1
Sequence conflict4511K → N in BAA22372. Ref.1
Sequence conflict5181V → A in BAA22372. Ref.1
Sequence conflict5511G → S in BAA22372. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q14534 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 6957F5CF1F1624C8

FASTA57463,923
        10         20         30         40         50         60 
MWTFLGIATF TYFYKKFGDF ITLANREVLL CVLVFLSLGL VLSYRCRHRN GGLLGRQQSG 

        70         80         90        100        110        120 
SQFALFSDIL SGLPFIGFFW AKSPPESENK EQLEARRRRK GTNISETSLI GTAACTSTSS 

       130        140        150        160        170        180 
QNDPEVIIVG AGVLGSALAA VLSRDGRKVT VIERDLKEPD RIVGEFLQPG GYHVLKDLGL 

       190        200        210        220        230        240 
GDTVEGLDAQ VVNGYMIHDQ ESKSEVQIPY PLSENNQVQS GRAFHHGRFI MSLRKAAMAE 

       250        260        270        280        290        300 
PNAKFIEGVV LQLLEEDDVV MGVQYKDKET GDIKELHAPL TVVADGLFSK FRKSLVSNKV 

       310        320        330        340        350        360 
SVSSHFVGFL MKNAPQFKAN HAELILANPS PVLIYQISSS ETRVLVDIRG EMPRNLREYM 

       370        380        390        400        410        420 
VEKIYPQIPD HLKEPFLEAT DNSHLRSMPA SFLPPSSVKK RGVLLLGDAY NMRHPLTGGG 

       430        440        450        460        470        480 
MTVAFKDIKL WRKLLKGIPD LYDDAAIFEA KKSFYWARKT SHSFVVNILA QALYELFSAT 

       490        500        510        520        530        540 
DDSLHQLRKA CFLYFKLGGE CVAGPVGLLS VLSPNPLVLI GHFFAVAIYA VYFCFKSEPW 

       550        560        570 
ITKPRALLSS GAVLYKACSV IFPLIYSEMK YMVH 

« Hide

References

« Hide 'large scale' references
[1]"Localization of the squalene epoxidase gene (SQLE) to human chromosome region 8q24.1."
Nagai M., Sakakibara J., Wakui K., Fukushima Y., Igarashi S., Tsuji S., Arakawa M., Ono T.
Genomics 44:141-143(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon endothelium.
[3]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Transcriptional regulation of squalene epoxidase by sterols and inhibitors in HeLa cells."
Nakamura Y., Sakakibara J., Izumi T., Shibata A., Ono T.
J. Biol. Chem. 271:8053-8056(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 187-535.
Tissue: Liver.
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78130 mRNA. Translation: BAA22372.1.
BX647400 mRNA. No translation available.
AC009908 Genomic DNA. No translation available.
FJ695197 Genomic DNA. No translation available.
D78129 mRNA. Translation: BAA11209.1.
RefSeqNP_003120.2. NM_003129.3.
UniGeneHs.71465.

3D structure databases

ProteinModelPortalQ14534.
SMRQ14534. Positions 118-181, 227-293.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112591. 4 interactions.
IntActQ14534. 3 interactions.
STRING9606.ENSP00000265896.

Chemistry

ChEMBLCHEMBL3592.
DrugBankDB01091. Butenafine.
DB00735. Naftifine.
DB00857. Terbinafine.

PTM databases

PhosphoSiteQ14534.

Polymorphism databases

DMDM296439362.

Proteomic databases

PaxDbQ14534.
PRIDEQ14534.

Protocols and materials databases

DNASU6713.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265896; ENSP00000265896; ENSG00000104549.
GeneID6713.
KEGGhsa:6713.
UCSCuc011liq.2. human.

Organism-specific databases

CTD6713.
GeneCardsGC08P126010.
H-InvDBHIX0007779.
HGNCHGNC:11279. SQLE.
HPAHPA018038.
HPA020762.
MIM602019. gene.
neXtProtNX_Q14534.
PharmGKBPA36108.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0654.
HOGENOMHOG000174713.
HOVERGENHBG005603.
InParanoidQ14534.
KOK00511.
OMAVMGVQYK.
OrthoDBEOG7J446G.
PhylomeDBQ14534.
TreeFamTF331056.

Enzyme and pathway databases

BioCycMetaCyc:HS02595-MONOMER.
ReactomeREACT_111217. Metabolism.
SABIO-RKQ14534.
UniPathwayUPA00767; UER00752.

Gene expression databases

ArrayExpressQ14534.
BgeeQ14534.
CleanExHS_SQLE.
GenevestigatorQ14534.

Family and domain databases

InterProIPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR003042. Rng_hydrolase-like.
IPR013698. Squalene_epoxidase.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF08491. SE. 1 hit.
[Graphical view]
PRINTSPR00420. RNGMNOXGNASE.
ProtoNetSearch...

Other

GeneWikiSqualene_monooxygenase.
GenomeRNAi6713.
NextBio26182.
PROQ14534.
SOURCESearch...

Entry information

Entry nameERG1_HUMAN
AccessionPrimary (citable) accession number: Q14534
Secondary accession number(s): Q9UEK6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM