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Protein

Helicase-like transcription factor

Gene

HLTF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.By similarity6 Publications

Miscellaneous

Subject to frequent epigenetic inactivation by promoter methylation in colon cancer.

Caution

In contrast with other SMARC proteins, there is currently no evidence that it associates with actin or actin-related proteins. It may rather act as a sequence-specific DNA binding ATPase, whose precise function remains to be fully characterized.Curated

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi38 – 287Add BLAST250
Nucleotide bindingi294 – 301ATPPROSITE-ProRule annotation8
Zinc fingeri760 – 801RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • protein ubiquitination Source: Reactome
  • regulation of transcription, DNA-templated Source: ProtInc

Keywordsi

Molecular functionActivator, Chromatin regulator, DNA-binding, Helicase, Hydrolase, Multifunctional enzyme, Transferase
Biological processTranscription, Ubl conjugation pathway
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
SIGNORiQ14527
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase-like transcription factor (EC:2.3.2.27, EC:3.6.4.-)
Alternative name(s):
DNA-binding protein/plasminogen activator inhibitor 1 regulator
HIP116
RING finger protein 80
RING-type E3 ubiquitin transferase HLTFCurated
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3
Sucrose nonfermenting protein 2-like 3
Gene namesi
Name:HLTF
Synonyms:HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000071794.15
HGNCiHGNC:11099 HLTF
MIMi603257 gene
neXtProtiNX_Q14527

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6596
OpenTargetsiENSG00000071794
PharmGKBiPA35949

Polymorphism and mutation databases

BioMutaiHLTF
DMDMi60390864

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000307221 – 1009Helicase-like transcription factorAdd BLAST1009

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27Omega-N-methylarginineCombined sources1
Cross-linki112Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei195Phosphotyrosine; by JAK21 Publication1
Cross-linki211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei397PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei736PhosphothreonineCombined sources1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ14527
MaxQBiQ14527
PaxDbiQ14527
PeptideAtlasiQ14527
PRIDEiQ14527

PTM databases

iPTMnetiQ14527
PhosphoSitePlusiQ14527

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSG00000071794
CleanExiHS_HLTF
ExpressionAtlasiQ14527 baseline and differential
GenevisibleiQ14527 HS

Organism-specific databases

HPAiHPA015284
HPA049001

Interactioni

Subunit structurei

Interacts with SP1 and SP3 independently of DNA; the interaction with these transcriptional factors may be required for basal transcription of target genes. Interacts with EGR1; the interaction requires prior binding to DNA and represses c-Rel via a DNA looping mechanism (By similarity). Interacts with GATA4 (By similarity). Interacts with PCNA; the interaction promotes polyubiquitination of PCNA through association with the UBE2B-RAD18 and UBE2V2-UBE2N ubiquitin ligase complexes. Interacts with RAD18, SHPRH, UBE2V2 and UBE2N.By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: MGI

Protein-protein interaction databases

BioGridi112480, 61 interactors
DIPiDIP-29828N
IntActiQ14527, 32 interactors
MINTiQ14527
STRINGi9606.ENSP00000308944

Structurei

Secondary structure

11009
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 54Combined sources4
Beta strandi58 – 68Combined sources11
Helixi70 – 72Combined sources3
Beta strandi81 – 87Combined sources7
Beta strandi98 – 101Combined sources4
Beta strandi107 – 111Combined sources5
Helixi113 – 124Combined sources12
Beta strandi129 – 134Combined sources6
Beta strandi141 – 152Combined sources12
Helixi154 – 156Combined sources3
Helixi157 – 166Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MZNNMR-A51-171[»]
4HREX-ray2.79G/H/K/L26-39[»]
4HRHX-ray3.00C/D26-39[»]
4S0NX-ray1.50A/B/C/D55-180[»]
4XZFX-ray1.38A58-174[»]
4XZGX-ray2.40A/B/C/D/E/F/G/H/I57-174[»]
5BNHX-ray1.70A/D55-175[»]
5K5FNMR-A51-171[»]
ProteinModelPortaliQ14527
SMRiQ14527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini435 – 606Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini837 – 996Helicase C-terminalPROSITE-ProRule annotationAdd BLAST160

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni925 – 1009Interaction with SP1 and SP31 PublicationAdd BLAST85

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi557 – 560DEGH box4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri760 – 801RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1001 Eukaryota
COG0553 LUCA
GeneTreeiENSGT00910000144305
HOVERGENiHBG079192
InParanoidiQ14527
KOiK15711
OMAiIMDNKLA
OrthoDBiEOG091G013T
PhylomeDBiQ14527
TreeFamiTF332703

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.30.40.10, 1 hit
3.40.50.10810, 2 hits
InterProiView protein in InterPro
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR014905 HIRAN
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF08797 HIRAN, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00910 HIRAN, 1 hit
SM00184 RING, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q14527-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWMFKRDPV WKYLQTVQYG VHGNFPRLSY PTFFPRFEFQ DVIPPDDFLT
60 70 80 90 100
SDEEVDSVLF GSLRGHVVGL RYYTGVVNNN EMVALQRDPN NPYDKNAIKV
110 120 130 140 150
NNVNGNQVGH LKKELAGALA YIMDNKLAQI EGVVPFGANN AFTMPLHMTF
160 170 180 190 200
WGKEENRKAV SDQLKKHGFK LGPAPKTLGF NLESGWGSGR AGPSYSMPVH
210 220 230 240 250
AAVQMTTEQL KTEFDKLFED LKEDDKTHEM EPAEAIETPL LPHQKQALAW
260 270 280 290 300
MVSRENSKEL PPFWEQRNDL YYNTITNFSE KDRPENVHGG ILADDMGLGK
310 320 330 340 350
TLTAIAVILT NFHDGRPLPI ERVKKNLLKK EYNVNDDSMK LGGNNTSEKA
360 370 380 390 400
DGLSKDASRC SEQPSISDIK EKSKFRMSEL SSSRPKRRKT AVQYIESSDS
410 420 430 440 450
EEIETSELPQ KMKGKLKNVQ SETKGRAKAG SSKVIEDVAF ACALTSSVPT
460 470 480 490 500
TKKKMLKKGA CAVEGSKKTD VEERPRTTLI ICPLSVLSNW IDQFGQHIKS
510 520 530 540 550
DVHLNFYVYY GPDRIREPAL LSKQDIVLTT YNILTHDYGT KGDSPLHSIR
560 570 580 590 600
WLRVILDEGH AIRNPNAQQT KAVLDLESER RWVLTGTPIQ NSLKDLWSLL
610 620 630 640 650
SFLKLKPFID REWWHRTIQR PVTMGDEGGL RRLQSLIKNI TLRRTKTSKI
660 670 680 690 700
KGKPVLELPE RKVFIQHITL SDEERKIYQS VKNEGRATIG RYFNEGTVLA
710 720 730 740 750
HYADVLGLLL RLRQICCHTY LLTNAVSSNG PSGNDTPEEL RKKLIRKMKL
760 770 780 790 800
ILSSGSDEEC AICLDSLTVP VITHCAHVFC KPCICQVIQN EQPHAKCPLC
810 820 830 840 850
RNDIHEDNLL ECPPEELARD SEKKSDMEWT SSSKINALMH ALTDLRKKNP
860 870 880 890 900
NIKSLVVSQF TTFLSLIEIP LKASGFVFTR LDGSMAQKKR VESIQCFQNT
910 920 930 940 950
EAGSPTIMLL SLKAGGVGLN LSAASRVFLM DPAWNPAAED QCFDRCHRLG
960 970 980 990 1000
QKQEVIITKF IVKDSVEENM LKIQNKKREL AAGAFGTKKP NADEMKQAKI

NEIRTLIDL
Length:1,009
Mass (Da):113,929
Last modified:March 1, 2005 - v2
Checksum:i0AB96F6C8484FD15
GO
Isoform 2 (identifier: Q14527-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.

Note: No experimental confirmation available.
Show »
Length:887
Mass (Da):99,919
Checksum:i7E4C330C2CF9DA4E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35P → L in CAA86571 (PubMed:8672239).Curated1
Sequence conflicti211 – 213KTE → PEF in AAB27691 (PubMed:8342330).Curated3
Sequence conflicti337D → G in CAA86571 (PubMed:8672239).Curated1
Sequence conflicti337D → G in CAA86572 (PubMed:8672239).Curated1
Sequence conflicti337D → G in CAD10805 (Ref. 3) Curated1
Sequence conflicti382S → T in AAA67436 (PubMed:7876228).Curated1
Sequence conflicti429Missing in AAH44659 (PubMed:15489334).Curated1
Sequence conflicti913K → R in CAA86571 (PubMed:8672239).Curated1
Sequence conflicti913K → R in CAA86572 (PubMed:8672239).Curated1
Sequence conflicti913K → R in CAD10805 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052121311N → S. Corresponds to variant dbSNP:rs2305868Ensembl.1
Natural variantiVAR_052122362E → Q. Corresponds to variant dbSNP:rs2228257Ensembl.1
Natural variantiVAR_029265819R → H. Corresponds to variant dbSNP:rs2229361Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188731 – 122Missing in isoform 2. 1 PublicationAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34673 mRNA Translation: AAA67436.1
Z46606 mRNA Translation: CAA86571.1
Z46606 mRNA Translation: CAA86572.1
AJ418064 Genomic DNA Translation: CAD10805.1
CH471052 Genomic DNA Translation: EAW78889.1
CH471052 Genomic DNA Translation: EAW78892.1
CH471052 Genomic DNA Translation: EAW78893.1
BC044659 mRNA Translation: AAH44659.1
S64671 mRNA Translation: AAB27691.1
CCDSiCCDS33875.1 [Q14527-1]
PIRiS49618
RefSeqiNP_001305863.1, NM_001318934.1
NP_001305864.1, NM_001318935.1 [Q14527-1]
NP_003062.2, NM_003071.3 [Q14527-1]
NP_620636.1, NM_139048.2 [Q14527-1]
XP_016862568.1, XM_017007079.1
UniGeneiHs.3068

Genome annotation databases

EnsembliENST00000310053; ENSP00000308944; ENSG00000071794 [Q14527-1]
ENST00000392912; ENSP00000376644; ENSG00000071794 [Q14527-1]
ENST00000494055; ENSP00000420429; ENSG00000071794 [Q14527-1]
GeneIDi6596
KEGGihsa:6596
UCSCiuc003ewq.2 human [Q14527-1]

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHLTF_HUMAN
AccessioniPrimary (citable) accession number: Q14527
Secondary accession number(s): D3DNH3
, Q14536, Q16051, Q7KYJ6, Q86YA5, Q92652, Q96KM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

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