Reviewed, UniProtKB/Swiss-Prot Q14457 (BECN1_HUMAN)

Names and origin

Protein names

Recommended name:
    Beclin-1
Alternative name(s):
    Coiled-coil myosin-like BCL2-interacting protein
    Protein GT197

Gene names

Name:	BECN1
Synonyms:	GT197

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a central role in autophagy By similarity. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Subunit structure	Interacts with GOPC and GRID2. Interacts with AMBRA1. Probably forms a complex with AMBRA1 and PIK3C3 By similarity. Interacts with BCL2 and BCL2L1.
Subcellular location	Cytoplasm Potential. Golgi apparatus membrane; Peripheral membrane protein By similarity. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells By similarity.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the beclin family.

Ontologies

Keywords
Biological process	Antiviral defense Autophagy
Cellular component	Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	anti-apoptosis Ref.1 Traceable author statement. Source: ProtInc autophagy Inferred from electronic annotation. Source: UniProtKB-KW cellular defense response Ref.1 Traceable author statement. Source: ProtInc response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 450

450

Beclin-1

PRO_0000218555

Regions

Coiled coil

142 – 270

129

Potential

Amino acid modifications

Modified residue

57

1

Phosphothreonine Ref.5

Modified residue

64

1

Phosphoserine Ref.5

Natural variations

Natural variant

103

1

A → V

VAR_010384

Natural variant

403

1

I → T

VAR_005236

Experimental info

Sequence conflict

150

1

T → A in AAB59573. Ref.4

Secondary structure

1

.

.

.

450

Helix Strand Turn

Details...

Helix

107 – 125

19

Sequences

	Sequence		Length	Mass (Da)
	Q14457-1 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: ABF0C2DD7087473C	FASTA	450	51,896
	10 20 30 40 50 60 MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE 70 80 90 100 110 120 ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG 130 140 150 160 170 180 DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL 190 200 210 220 230 240 QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ 250 260 270 280 290 300 LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW 310 320 330 340 350 360 NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF 370 380 390 400 410 420 WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN 430 440 450 SEEQWTKALK FMLTNLKWGL AWVSSQFYNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." Liang X.H., Kleeman L.K., Jiang H.H., Gordon G., Goldman J.E., Berry G., Herman B., Levine B. J. Virol. 72:8586-8596(1998) [PubMed: 9765397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH BCL-2. Tissue: Brain.
[2]	"Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." Aita V.M., Liang X.H., Murty V.V.V.S., Pincus D.L., Yu W., Cayanis E., Kalachikov S., Gilliam T.C., Levine B. Genomics 59:59-65(1999) [PubMed: 10395800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[4]	"Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21." Rommens J.M., Durocher F., McArthur J., Tonin P., Leblanc J.-F., Allen T., Samson C., Ferri L., Narod S., Morgan K., Simard J. Genomics 28:530-542(1995) [PubMed: 7490091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 150-450. Tissue: Mammary gland.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-57 AND SER-64, MASS SPECTROMETRY.
[6]	"Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein." Oberstein A., Jeffrey P.D., Shi Y. J. Biol. Chem. 282:13123-13132(2007) [PubMed: 17337444] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 107-135 IN COMPLEX WITH BCL2L1.
[7]	"Molecular basis of Bcl-xL's target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of beclin-1." Feng W., Huang S., Wu H., Zhang M. J. Mol. Biol. 372:223-235(2007) [PubMed: 17659302] [Abstract] Cited for: STRUCTURE BY NMR OF 106-128 IN COMPLEX WITH BCL2L1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF077301 mRNA. Translation: AAC68653.1.
AF139131 mRNA. Translation: AAD27650.1.
BC010276 mRNA. Translation: AAH10276.1.
L38932 mRNA. Translation: AAB59573.1.

IPI

IPI00748342.

PIR

I54209.

RefSeq

NP_003757.1.

UniGene

Hs.12272

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P1L	X-ray	2.50	B/D/F/H	107-135	[»]
2PON	NMR	-	A	106-128	[»]
3DVU	X-ray	2.50	C/D	105-130	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q14457. 1 interaction.

PTM databases

PhosphoSite

Q14457.

Proteomic databases

PRIDE

Q14457.

Genome annotation databases

Ensembl

ENSG00000126581. Homo sapiens. [Contig view]

GeneID

8678.

KEGG

hsa:8678.

Organism-specific databases

GeneCards

GC17M038215.

H-InvDB

HIX0018211.

HGNC

HGNC:1034. BECN1.

HPA

CAB010143.

MIM

604378. gene.

PharmGKB

PA25337.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q14457.

OMA

Q14457. GTMENLS.

Gene expression databases

ArrayExpress

Q14457.

Bgee

Q14457.

CleanEx

HS_BECN1.

GermOnline

ENSG00000126581. Homo sapiens.

Family and domain databases

InterPro

IPR007243. Autophagy-rel_prot_6.
[Graphical view]

PANTHER

PTHR12768. APG6. 1 hit.

Pfam

PF04111. APG6. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

32555.

SOURCE

Search...

Entry information

Entry name

BECN1_HUMAN

Accession

Primary (citable) accession number: Q14457
Secondary accession number(s): O75595, Q9UNA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 21, 2001
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)